THE SONOGRAPHIC DIAGNOSIS OF CHRONIC PROLIFERATIVE SYNOVITIS IN THE METACARPOPHALANGEAL JOINTS OF A HORSE     

Phillip F.  Steyn  BSc  BVSc  MRCVS  David  Schmitz  DVM  MS  Jeffrey  Watkins  DVM  MS  Jay  Hoffman  BS  DVM 《Veterinary radiology & ultrasound》1989,30(3):125-127

Chronic proliferative synovitis is an insidious condition affecting the metacarpophalangeal joints of horses. It results in proliferation of the synovium in the dorsal pouch of the joint in question. Treatment involves surgical excision, thus confirmation of the lesion is important. Classically this has been achieved by means of positive-contrast arthrography, but this article addresses the attributes of ultrasonography, a less invasive imaging technique.  相似文献   

Evaluation of esophageal transit of tablets and capsules in 30 cats     

Westfall DS  Twedt DC  Steyn PF  Oberhauser EB  VanCleave JW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(5):467-470

We have reported tablet-induced focal esophagitis and esophageal stricture formation in cats. The proposed mechanism is thought to be abnormal esophageal tablet retention resulting in focal esophagitis with subsequent stricture formation. The objective of this study was to evaluate the passage of tablets and capsules when given alone (dry swallow) and when followed by a water bolus (wet swallow) to determine if this could, in part, explain the esophageal stricture formation we have observed in cats. Fluoroscopy was used to evaluate tablet or capsule passage after administration. The percentage of dry tablet swallows that successfully passed into the stomach was 0.0% at 30 and 60 seconds, 6.7% at 90 seconds, 13.3% at 120 seconds, 26.7% at 180 and 240 seconds, and 36.7% at 300 seconds. Wet tablet swallows successfully passed 90.0% of the time at 30 seconds, 93.3% of the time at 60 seconds, and 100.0% of the time thereafter. The percentage of dry capsule swallows that successfully passed was 16.7% at each time interval. Wet capsule swallows successfully passed 96.7% of the time at 30 seconds and 100% of the time thereafter. For each time interval, wet swallows achieved significantly greater percentage passage into the stomach when compared to dry swallows (P < .05). This study shows that tablets or capsules given by dry swallow have prolonged retention in the esophagus compared to those given by wet swallow. On the basis of this study, we recommend the routine administration of a water bolus to cats receiving tablets or capsules PO to facilitate esophageal clearance. This practice may help prevent medication-associated esophagitis or stricture formation.  相似文献   

Toe grabs     

HC 《Journal of Equine Veterinary Science》1989,9(6):334

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A field screening technique for drought tolerance studies in potatoes     

J. M. Steyn  H. F. Du Plessis  P. S. Hammes 《Potato Research》1998,41(3):295-303

Summary A major objective of plant breeding programmes in semi-arid conditions is the selection of more drought-tolerant plant material. An irrigation system has been developed to assist in water use and drought tolerance screening studies of potato genotypes in the confined space of a rain shelter. The line-source principle was used as a departure point for the design of an irrigation boom, attached to the roof structure of a rain shelter. Five water regimes were achieved by using nozzles that differed in discharge rate and three genotypes per rain shelter were evaluated simultaneously. Crop production functions could be established for the different genotypes. The system shares some disadvantages of the typical line-source, such as the simultaneous irrigation of all plots and water treatments are not randomised. The main advantage above traditional plot systems, which would use drip or micro irrigation under rain shelters, is the ease of management.  相似文献   

Phase-matched generation of coherent soft X-rays     

A Rundquist  CG Durfee  Z Chang  C Herne  S Backus  MM Murnane  HC Kapteyn 《Science (New York, N.Y.)》1998,280(5368):1412-1415

Phase-matched harmonic conversion of visible laser light into soft x-rays was demonstrated. The recently developed technique of guided-wave frequency conversion was used to upshift light from 800 nanometers to the range from 17 to 32 nanometers. This process increased the coherent x-ray output by factors of 10(2) to 10(3) compared to the non-phase-matched case. This source uses a small-scale (sub-millijoule) high repetition-rate laser and will enable a wide variety of new experimental investigations in linear and nonlinear x-ray science.  相似文献   

Overweight among children decreased, but obesity prevalence remained high among women in South Africa, 1999-2005     

Kruger HS  Steyn NP  Swart EC  Maunder EM  Nel JH  Moeng L  Labadarios D 《Public health nutrition》2012,15(4):594-599

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Effects of Prolonged in vitro Culture and Cryopreservation on Viability,DNA Fragmentation,Chromosome Stability and Ultrastructure of Bovine Cells from Amniotic Fluid and Umbilical Cord     

ER da Cunha  CF Martins  CG Silva  HC Bessler  SN Báo 《Reproduction in domestic animals》2014,49(5):806-812

The objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long‐term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that bovine AFCs and UCCs can be isolated, cultured in vitro and cryopreserved in 10% DMSO, not causing damage to DNA and chromosomes. The UCCs were more resistant than AFCs in all aspects.  相似文献   

Effect of Long-Term in Vitro Sub-culturing on Quality Degeneration of Sweet Potato Varieties： Morpho- Anatomic Assessment and Simple Sequence Repeats Analysis     

Mihiretu Cherinet Hundayehu  ;Elsa Du Toit  ;Sunette Laurie  ;Martin Steyn  ;Ria Greyling  ;Nokuthula Myeza 《农业科学与技术》2014,(10):811-821

The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog （MS）, 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat （SSR） primers （IB-242, IB-318, IB-255F, 1B-248 and IB-255）. Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties.  相似文献   

Nuclear scanning with ^99mTc-HDP for the initial evaluation of osseous metastasis in canine osteosarcoma     

M. K. Jankowski  P. F. Steyn  S. E. Lana  W. S. Dernell  C. M. Blom  J. L. Uhrig  M. Lafferty  S. J. Withrow 《Veterinary and comparative oncology》2003,1(3):152-158

The purpose of this retrospective analysis was to evaluate the use of nuclear scintigraphy in determining the rate of secondary sites of osseous malignancy at initial presentation in dogs with osteosarcoma. Radiographs of suspicious secondary lesions were reviewed and placed into four separate categories: benign lesions; no lesion seen on radiographs; subtle radiographic changes suggestive of, but not conclusive for, metastasis; and metastatic lesions highly suspected on radiographs. Three hundred and ninety‐nine dogs were evaluated by technetium nuclear scanning for suspected osteosarcoma. Three hundred and twenty‐six of 399 dogs (82%) had only one apparent site on the nuclear scan, whereas 72 dogs (18%) had more than one suspicious site on the nuclear scans. Highly suspected secondary metastatic lesions were detected by nuclear scans in 7.8% of cases. Although interpretation of nuclear scans is subjective, this study showed a 7.8% chance of detecting unsuspected osseous metastasis with nuclear scans in canine osteosarcoma patients on initial presentation.  相似文献   

Prevalence and determinants of stunting and overweight in 3-year-old black South African children residing in the Central Region of Limpopo Province, South Africa     

Mamabolo RL  Alberts M  Steyn NP  Delemarre-van de Waal HA  Levitt NS 《Public health nutrition》2005,8(5):501-508

OBJECTIVES: To determine the prevalence of stunting, wasting and overweight and their determinants in 3-year-old children in the Central Region of Limpopo Province, South Africa. DESIGN: Prospective cohort study. SETTING: Rural villages in the Central Region of the Limpopo Province, South Africa. SUBJECTS: One hundred and sixty-two children who were followed from birth were included in the study. Anthropometric measurements and sociodemographic characteristics of the children were recorded. RESULTS: Height-for-age Z-scores were low, with a high prevalence of stunting (48%). The children also exhibited a high prevalence of overweight (22%) and obesity (24%). Thirty-one (19%) children were both stunted and overweight. Gaining more weight within the first year of life increased the risk of being overweight at 3 years by 2.39 times (95% confidence interval (CI) 1.96-4.18) while having a greater length at 1 year was protective against stunting (odds ratio (OR) 0.41; 95% CI 0.17-0.97). Having a mother as a student increased the risk for stunting at 3 years by 18.21 times (95% CI 9.46-34.74) while having a working mother increased the risk for overweight by 17.87 times (95% CI 8.24-38.78). All these factors also appeared as risks or as being protective in children who were both overweight and stunted, as did living in a household having nine or more persons (OR 5.72; 95% CI 2.7-12.10). CONCLUSION: The results of this study highlight the importance of evaluating anthropometric status in terms of both stunting and overweight. Furthermore, it is important to realise the importance of normal length and weight being attained at 1 year of age, since these in turn predict nutritional status at 3 years of age.  相似文献