Epidemiological studies of equine herpesvirus 1 (EHV-1) in Thoroughbred foals: a review of studies conducted in the Hunter Valley of New South Wales between 1995 and 1997.     

J R Gilkerson  J M Whalley  H E Drummer  M J Studdert  D N Love 《Veterinary microbiology》1999,68(1-2):15-25

Sero-epidemiological studies conducted between 1995 and 1997 on two large Thoroughbred stud farms in the Hunter Valley of NSW showed clear evidence of EHV-1 infection in foals as young as 30 days of age. Similarly, serological evidence suggested that these foals were infected with EHV-1 from their dams or from other lactating mares in the group, with subsequent foal to foal spread of infection prior to weaning. These studies also provided evidence of EHV-1 infection of foals at and subsequent to weaning, with foal to foal spread of EHV-1 amongst the weanlings. These data indicated that the mare and foal population was a reservoir of EHV-1, from which new cases of infection propagated through the foal population both before and after weaning. The results of these studies support the long standing management practices of separating pregnant mares from other groups of horses to reduce the incidence of EHV-1 abortion. Also, these results have important implications for currently recommended vaccination regimens, as the efficacy of vaccination in already latently infected horses is unknown.  相似文献   

Sites of Persistence of Lumpy Skin Disease Virus in the Genital Tract of Experimentally Infected Bulls     

CH Annandale  PC Irons  VP Bagla  UI Osuagwuh  EH Venter 《Reproduction in domestic animals》2010,45(2):250-255

The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate.  相似文献   

Soil organic carbon in cropping sequences with predominance of soya bean in the argentinean humid Pampas     

Juan P. Martinez  Cecilia Crespo  Hernán Sainz Rozas  Hernán Echeverría  Guillermo Studdert  Fernado Martinez  Graciela Cordone  Pablo Barbieri 《Soil Use and Management》2020,36(1):173-183

Soya bean (Glycine max (L.) Merr.) monoculture can lead to a decrease in labile fractions of soil organic carbon (SOC). This study sought to evaluate the effects of cover crops (CC), application of fertilizer, and crop rotation on SOC, particulate organic carbon (POC), and soil carbon input in soya bean-based crop sequences under a no-till cropping system in the Argentinean Humid Pampas. Five crop sequences at two sites differing in initial SOC were evaluated: continuous soya bean (Sb), continuous soya bean fertilized with phosphorus (P) and sulphur (S) (Sbf), grass CC / PS-fertilized soya bean (CC/Sbf), nitrogen (N)-fertilized CC / PS-fertilized soya bean (CCf/Sbf) and NPS-fertilized crop rotation with high intensification sequence index (ISI) (Rot). At 0–5 cm, SOC and POC were higher (p < .05) in the sequences with higher residue-C supply (CC/Sbf; CCf/Sbf and Rot) at both sites. Changes in SOC at 0–20 cm simulated by AMG model closely tracked measured results at 0–20 cm. Findings from this study suggest that the inclusion of CC or crop rotation with high ISI improved C balance in soils under crop sequences with soya bean predominance.  相似文献   

Restriction enzyme maps for equine adenovirus 1 genome.     

M Sheppard  S M Drysdale  M J Studdert 《Veterinary microbiology》1992,31(4):343-349

Physical maps were constructed for the genome of equine adenovirus 1 (EAV1) using the restriction enzymes; DraI, EcoRV, NotI and SfiI. The total size of the EAV1 genome was 34.4 kb estimated by comparison with known DNA standards and the polarity of the fragment order, with respect to the left and right molecular ends, was determined by hybridization with known regions of the human adenovirus 2 (HAV2) genome.  相似文献   

Equine herpesviruses causing respiratory disease     

Studdert MJ 《New Zealand veterinary journal》1986,34(7):118

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Diagnosis of feline leukemia virus and feline immunodeficiency virus infections     

B W Parry  S A Holloway  M J Studdert 《Veterinary Clinics of North America: Small Animal Practice》1989,19(4):719-727

Feline leukemia virus is an oncogenic retrovirus that can result in a wide variety of neoplastic and non-neoplastic diseases, including immunosuppression. Diagnosis of FeLV infection can be achieved by several methods, including virus isolation; IFA assay of a peripheral blood smear; and detection of a viral protein (called p27) by ELISA testing of whole blood, plasma, serum, saliva, or tears. Commercially available ELISA kits have revolutionized FeLV testing and have become very popular as "in-house" procedures. This article discusses the interpretation of ELISA results and compares them with IFA assay findings. Feline immunodeficiency virus is a lentivirus that causes immunosuppression, but not neoplasia, in cats. It originally was called feline T-lymphotropic lentivirus. Differentiating FIV infection from the immunosuppressive type of FeLV infection requires virus isolation or serology. The most rapid method for diagnosis of FIV infection is ELISA testing for antiviral antibody.  相似文献   

The history of arboviral infections of horses in Australia is being rewritten     

Michael J Studdert 《Australian veterinary journal》2013,91(1-2):4-4

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West Nile virus revisited and other mosquito borne viruses of horses in Australia     

Studdert MJ 《Australian veterinary journal》2003,81(1-2):56-57

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David Ogilvie White     

MJ Studdert  R. Robins‐Browne 《Australian veterinary journal》2005,83(5):304-304

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Pulsatile Secretory Pattern of Gonadotrophins and Ovarian Steroids during the Periovulatory Phase of the Oestrous Cycle in the Buffalo     

Baljit Singh  VD Dixit  VP Dixit  IS Lohan  GC Georgie 《Reproduction in domestic animals》1998,33(5):363-366

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