Cis-acting elements analysis and function detection of xylem specific promoter from <Emphasis Type="Italic">Populus</Emphasis>     

Hui Wu  Shuang Zhang  Zhigang Wang  Jinhao Su  Kejiu Du 《Frontiers of Agriculture in China》2010,4(2):226-231

The cis-acting elements of JCesAP, YCesAP, and MDCesAP were analyzed by the PLACE database, the results showed that three sequences all had a core element and upstream promoter element containing TATA-box and CAAT-box. The sequences related xylem-specific and wound-defense functions were found in YCesAP and MDCesAP; and the “core xylem gene set” in JCesAP, indicating that three sequences were xylem-specific and provided with wound-defense functions. Meanwhile, some other elements were also detected, including some tissue-specific elements and some regulative ones that were induced by light, temperature, and water. For investigating their functions, three sequences of JCesAP, YCesAP, and MDCesAP were respectively combined with the report gene GFP, and those reconstructed GFP genes were respectively transferred into the genome of Populus tomentose by Agrobacterium-mediated transformation method. The detected results of GFP expressions in hygromycin B resistant calli of P. tomentosa showed that three sequences all possessed the promoter functions with tissue-specific characteristics.  相似文献   

不同裸燕麦品种的淀粉特性   总被引：4，自引：1，他引：3  

王燕  易翠平  王强  周素梅 《麦类作物学报》2010,30(3):560-563

为给燕麦淀粉的应用以及适宜加工品种的筛选提供依据,从国内主栽燕麦中选取10个品种的11个样品(其中坝莜1号有河北和山西产两种样品)作为供试材料,通过湿法提取燕麦淀粉,并对其组成、黏度及凝胶特性进行了对比分析.结果显示,不同裸燕麦品种的总淀粉含量和直链淀粉含量变幅分别为91.06%～95.98%和21.06%～31.26%,品种间总淀粉变异较小,而直链淀粉变异较大.淀粉糊化特征中,样品间淀粉糊化温度变异最小,平均为82.0℃,反映淀粉糊热稳定性的破损值差异最大(变异系数23.8%).在淀粉的凝胶特性指标中,黏附性差异最为显著(变幅0.00～139.89 g·s),其次为硬度(9.87～43.76 g),弹性、黏聚性差别不显著.11个材料中,坝莜1号(河北产)淀粉糊黏度大.热稳定性好;晋八属于易回生、强凝胶型.  相似文献   

鸡传染性法氏囊病病毒SYBR Green Ⅰ实时荧光定量RT-PCR检测方法的建立     

朱春华  林甦  程龙飞  陈红梅  陈珍  刘斌琼  蔡国漳  林羽  黄瑜 《福建农业学报》2011,26(2):175-179

R-PCR扩增IBDV的VP5基因保守片段,将其克隆到pMD18-T载体,经测序鉴定得到阳性质粒.以阳性质粒为标准品,建立SYBR Green Ⅰ实时荧光定量RT-PCR的标准曲线和溶解曲线.结果表明,IBDV荧光定量PCR的标准曲线Ct值与3.29×10～3.29×108之间的病毒基因拷贝数呈现良好的线性关系.该方法...  相似文献   

福建省蛋鸡血管瘤型J亚群白血病的分子生物学诊断     

林甦  陈珍  朱春华  刘斌琼  施少华  蔡国漳  程龙飞  黄瑜 《福建农业学报》2011,26(1):6-9

针对福建省某鸡场的蛋鸡群发生产蛋下降和部分死亡,病鸡脚趾明显呈现血管瘤特征,运用实时荧光定量PCR方法对病鸡组织和血液样品进行检测,检出了J亚群白血病病毒(ALV-J);通过基因序列的比较分析,发现其氨基酸序列与国外及国内其他省份报导的ALV-J有很高的同源性,从病原分子生物学诊断角度证实福建省蛋鸡群中ALV-J的存在。  相似文献   

Generating and reducing video summary with adaptive CCV and equivalent relation clustering     

LUO Bin  DAI Yu ming  ZHAI Su lan 《保鲜与加工》2010,(2):69-72

It is very essential to extract representative frames in the process of generating video summary. A method is proposed which analyses the color features of the video frames, sets the connectivity threshold values automatically according to the contents, extracts the color coherence vectors (CCV) and then performs adaptive clustering based on equivalent relation. After global partition, local partition was revised with time sequential features. The whole process does not need to set any threshold values. The experiments with diverse videos yields effective results.  相似文献   

利用谷氨酰胺转氨酶和结冷胶提高鱼丸凝胶强度的研究   总被引：1，自引：0，他引：1  

苏德福  林向阳  吴求林  陈兴才 《农产品加工．学刊》2010,(1):37-39

用磷酸盐提高鱼丸凝胶强度的效果显著,但根据GB2760规定,用量有限。采用谷氨酰胺转氨酶(TG酶)和结冷胶替代磷酸盐,优化配方为:谷氨酰胺转氨酶添加量为0.4%,结冷胶添加量为0.5%,产品的凝胶强度达到使用磷酸盐的1.9倍。  相似文献   

高职食品安全与检验技术类学习领域课程构建的研究   总被引：2，自引：2，他引：0  

王尔茂  贡汉坤  江建军  苏新国  杨天英 《农产品加工．学刊》2010,(4):89-91

"食品质量安全控制与管理"和"食品检测技术"是高职高专院校食品安全与检验技术专业的两个重要学习领域与食品检验和食品生产现场质量安全控制岗位相对接是食品类专业教学改革的关键与难点。在教育部高职高专食品类教学指导委员会的指导下,构建了"食品质量安全控制与管理"和"食品检测技术"两门课程的设置,为规范高职高专院校食品安全与检验技术类专业提供了支撑。  相似文献   

马铃薯生料发酵制备乙醇的研究   总被引：1，自引：0，他引：1  

苏小军  熊兴耀  谭兴和  蔡柳  高艳  李文佳 《农产品加工．学刊》2010,(6):8-11

比较了商品复合酶和生淀粉酶对马铃薯生料直接发酵转化乙醇的影响,60Co-γ射线辐照处理马铃薯对乙醇发酵的影响。结果表明,以生淀粉酶为糖化剂的发酵液中乙醇体积分数远远高于商品复合酶的乙醇体积分数,辐照处理可显著提高马铃薯生料转化乙醇的能力。  相似文献   

The detection of “Candidatus Mycoplasma haemobos” in cattle and buffalo in China     

Su  Q. L.  Song  H. Q.  Lin  R. Q.  Yuan  Z. G.  Yang  J. F.  Zhao  G. H.  Huang  W. Y.  Zhu  Xing Quan 《Tropical animal health and production》2010,42(8):1805-1808

“Candidatus Mycoplasma haemobos” is a hemoplasma species found in cattle and has been recently reported in Switzerland and Japan. In this study, “Candidatus Mycoplasma haemobos” was shown to occur in cattle and buffalo in tropical China by PCR amplification and sequence analysis of the 16S rRNA gene from blood samples. Based on the 16S rDNA sequence, a specific PCR assay was developed. Occurrence of “Candidatus Mycoplasma haemobos” in cattle and buffalo in Guangxi, China, was determined by examining 25 buffalo blood samples, 12 yellow cattle blood samples and 42 dairy cow blood samples. The results showed that 32% (8/25) of buffalo, 41.7% (5/12) of yellow cattle, and 14.3% (6/42) of dairy cows were positive for “Candidatus Mycoplasma haemobos”, respectively. Direct sequencing of representative PCR products confirmed that the amplified partial 16S rDNA sequence represented “Candidatus Mycoplasma haemobos”. This is the first report of “Candidatus Mycoplasma haemobos” in buffalo, yellow cattle, and dairy cows in China.  相似文献   

Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124   总被引：1，自引：0，他引：1  

Kuhnert F  Mancuso MR  Shamloo A  Wang HT  Choksi V  Florek M  Su H  Fruttiger M  Young WL  Heilshorn SC  Kuo CJ 《Science (New York, N.Y.)》2010,330(6006):985-989

The orphan G protein-coupled receptor (GPCR) GPR124/tumor endothelial marker 5 is highly expressed in central nervous system (CNS) endothelium. Here, we show that complete null or endothelial-specific GPR124 deletion resulted in embryonic lethality from CNS-specific angiogenesis arrest in forebrain and neural tube. Conversely, GPR124 overexpression throughout all adult vascular beds produced CNS-specific hyperproliferative vascular malformations. In vivo, GPR124 functioned cell-autonomously in endothelium to regulate sprouting, migration, and developmental expression of the blood-brain barrier marker Glut1, whereas in vitro, GPR124 mediated Cdc42-dependent directional migration to forebrain-derived, vascular endothelial growth factor-independent cues. Our results demonstrate CNS-specific angiogenesis regulation by an endothelial receptor and illuminate functions of the poorly understood adhesion GPCR subfamily. Further, the functional tropism of GPR124 marks this receptor as a therapeutic target for CNS-related vascular pathologies.  相似文献