Careers in science. More women in science     

Handelsman J  Cantor N  Carnes M  Denton D  Fine E  Grosz B  Hinshaw V  Marrett C  Rosser S  Shalala D  Sheridan J 《Science (New York, N.Y.)》2005,309(5738):1190-1191

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Whole animal cell sorting of Drosophila embryos   总被引：2，自引：0，他引：2  

M A Krasnow  S Cumberledge  G Manning  L A Herzenberg  G P Nolan 《Science (New York, N.Y.)》1991,251(4989):81-85

Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic beta-galactosidase substrate and fluorescence-activated cell sorting. With WACS, incipient posterior compartment cells that express the engrailed gene were purified from early Drosophila embryos. Neuronal precursor cells were also purified, and they differentiated into neurons with high efficiency in culture. Because there are many lacZ strains, it may be possible to purify most types of Drosophila cells. The same approach is also applicable to other organisms for which germ-line transformation is possible.  相似文献   

Competencies: a cure for pre-med curriculum     

Anderson WA  Amasino RM  Ares M  Banerjee U  Bartel B  Corces VG  Drennan CL  Elgin SC  Epstein IR  Fanning E  Guillette LJ  Handelsman J  Hatfull GF  Hoy RR  Kelley D  Leinwand LA  Losick R  Lu Y  Lynn DG  Neuhauser C  O'Dowd DK  Olivera T  Pevzner P  Richards-Kortum RR  Rine J  Sah RL  Strobel SA  Walker GC  Walt DR  Warner IM  Wessler S  Willard HF  Zare RN 《Science (New York, N.Y.)》2011,334(6057):760-761

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Occurrence of Honeysuckle Yellow Vein Virus (HYVV) containing a monopartite DNA-A genome in Korea     

Yuan Wang  Jing Ji  Tae-Kyun Oh  Sung Oh  Sue Hoon Kim  Hyun Ju Lee  Myoung Yong Shim  Chang Won Choi  Seong Hwan Kim  Il-Seop Kim  Young Shik Kim 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(3):361-370

Two newly emerged begomoviruses were isolated from naturally infected tomato (Solanum lycopersicum) plants grown in greenhouses at Jeju Island and Dangjin in Korea and their genomes were characterized. These viruses-infected plants had very small leaves that curled upward, yellow margins and a leathery appearance, and a bushy and stunted appearance with short internodes. Nucleotide (nt) sequence analysis of their genomes showed that they have a DNA-A component of a monopartite begomovirus. Their genomes comprised 2763 and 2764 nucleotides with six open reading frames. The results of nt sequence similarity analysis of DNA-A genome between the two Korean isolates and isolates of Tobacco leaf curl Japan virus (TbLCJV), Honeysuckle yellow vein virus (HYVV), Honeysuckle yellow vein mosaic virus (HYVMV), and Eupatorium yellow vein virus in Japan (EpYVV) showed that they are likely similar to HYVV-[Masuda] (89.4–92.8% nt identity). Consequently, we tentatively propose the two isolates’ names as HYVV-Jeju and -DJ according to the ICTV geminivirus rules. Phylogenetic relationship analysis of 33 DNA-A genome sequences using PAUP* 4.0b10 and MrBayes revealed that HYVV-Jeju and -DJ belong to the Far East Asian begomovirus species complex. Within the Far East Asian begomovirus species complex, HYVV-Jeju and -DJ are distantly related to EpYVV, HYVMV, and TbLCJV groups. Based on the presence of a recombination fragment spanning the C3 ORF, a recombinant origin was suggested for both HYVV-Jeju and –DJ, with parents close to Japanese isolates HYVMV-[SP1:00] and Eupatorium yellow vein virus (EpYVV)-[Suya]. In addition, the presence of a further recombination fragment spanning the IR suggested the parents of HYVV-DJ were close to HYVV-Jeju and EpYVV-[Suya].  相似文献   

Handbook of Clay Science (Developments in Clay Science, 1) - Edited by F. Bergaya, B.K.G. Theng & G. Lagaly     

D.A.C. Manning 《European Journal of Soil Science》2007,58(2):518-519

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Volatile constituents of fermented sugar baits and their attraction to lepidopteran species     

El-Sayed AM  Heppelthwaite VJ  Manning LM  Gibb AR  Suckling DM 《Journal of agricultural and food chemistry》2005,53(4):953-958

The volatile compounds emanating from four fermented sugar baits, palm sugar, golden cane syrup, port wine, and molasses, were isolated by headspace sampling and analyzed by gas chromatography-mass spectrometry. Three classes of compounds including esters, alcohols, and aromatic compounds were identified in the headspace of the four fermented sugar baits. There was a high degree of qualitative similarity between the headspace contents of the four fermented sugar baits, although quantitatively they varied considerably. Ethyl acetate, 3-methylbutanol, ethyl hexanoate, 2-phenylethanol, ethyl octanoate, ethyl (E)-4-decenoate, ethyl decanoate, and ethyl dodecanoate were the major compounds identified in the headspace of the four fermented sugar baits. The efficacy of the four fermented sugar baits was investigated in field trapping experiments. Fermented palm sugar and golden cane syrup were superior in attracting significant numbers of moths as compared to port wine and molasses. Fermented molasses was the least attractive among the four baits. Over 90% of the insects caught were noctuids with Graphania mutans and Tmetolophota spp. being the main noctuids captured (over 55%) in the four fermented sugar baits. Male and female G. mutans were equally attracted to the four sugar baits. A number of tortricid species were also trapped.  相似文献   

Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats   总被引：1，自引：0，他引：1       下载免费PDF全文

Elizabeth J.B. Manning  Howard Steinberg  Vernon Krebs    Michael T. Collins 《Canadian journal of veterinary research》2003,67(3):213-218

Thirteen pygmy goats (Capra hircus) from a herd naturally infected with Mycobacterium avium ss. paratuberculosis (MPTB) were monitored with 4 diagnostic assays for 2 to 15 mo. Cellular and humoral immune responses to the infection were assessed with assays of gamma interferon (IFNγ), serum antibody [enzyme-linked immunosorbent assay (ELISA) and agar gel diffusion (AGID)], and radiometric fecal culture. Microscopic examination and radiometric culture of tissue from 12 sites were performed at necropsy. Goats were considered infected if MPTB was isolated from any tissue sample collected at necropsy. Mycobacterial isolates were confirmed as MPTB with an IS900 polymerase chain reaction assay. Ten goats whose antemortem tests indicated infection carried heavy organism burdens at necropsy, both within and beyond the gastrointestinal system. False-negative ELISA, AGID, and/or culture results were obtained in 5 of the 10 confirmed cases during the study period. In 3 goats with sporadic fecal shedding of MPTB or detectable IFNγ response, or both, no abnormalities were detected at necropsy and no MPTB was isolated from the tissue samples; the antemortem fecal-culture and IFNγ results were thus considered false-positive. Diagnosticians should be alert to the possibility of both false-positive and false-negative test results for Johne's disease in goats. False-positive fecal-culture results may occur when a high prevalence of infection exists in the herd and the premises are likely to be heavily contaminated. The diverse antemortem testing patterns seen in these goats underscore the importance of using varied diagnostic assays serially or in parallel to increase the likelihood of identifying all infected goats.  相似文献   

Bacteremia concomitant with parvovirus infection in a pup     

T J Kreeger  K P Jeraj  P J Manning 《Journal of the American Veterinary Medical Association》1984,184(2):196-197

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Failure of procaine penicillin and zinc bacitracin to modify the response of the fowl to stressors.     

B M Freeman  A C Manning 《British poultry science》1976,17(3):285-292

1. The effect of either procaine penicillin or zinc bacitracin (25 mg active base/kg food) on the response of young birds to three different stressors - withdrawal of food for 18 h, exposure to cold for 24 h or treatment with ACTH- has been examined. 2. Neither antibiotic had any consistent effect on the stress responses. 3. It is concluded that neither penicillin nor bacitracin has any stress-ameliorating activity at least under the conditions of the test.  相似文献   

Characterization of the sorption of an anthranilate fungicide in soil using thermal analytical and mineralogical techniques     

Kennedy A  Lopez-Capel E  Manning DA  Wilkins RM 《Pest management science》2005,61(7):705-714

The sorption of pesticides to soil particles has implications for their distribution and fate in the soil environment. A batch equilibrium technique was used to investigate sorption of the fungicide AEC623892 to intact and hydrogen-peroxide-treated whole soils and several particle-size fractions isolated from them. Sorption of AEC623892 to the soil as a whole was low. K(f oc) values measured in the whole soils were 169.2 and 41.9 ml g(-1) for Soil A and Soil B respectively. The highest values of K(f oc) were measured in soil particle-size fractions <53 microm (266.5 ml g(-1) in the 2-20 microm fraction of Soil A; 471.9 ml g(-1) in the 20-53 microm fraction of Soil B). Sorption was most irreversible in the 2-20 microm fractions. Overall, treatment of soil particle-size fractions with hydrogen peroxide resulted in lower values of K(f oc) (112.3 ml g(-1) in Soil A whole soil and 30.9 ml g(-1) in Soil B whole soil). In both soils, the maximum sorption among hydrogen-peroxide-treated samples was observed in the <2 microm fraction (166.6 and 311.0 ml g(-1) for Soil A and Soil B, respectively). Investigation of the mineralogical composition of the soils suggested that the clay mineralogy (dominated by kaolinite and illite) is less likely to account for the differences in sorption observed than differences in the characteristics of the soil organic matter. Thermal analysis of the different soil fractions indicated that hydrogen peroxide treatment preferentially removed aliphatic fractions of organic matter, but had less effect on lignin-like, aromatic fractions.  相似文献