Effectiveness of capillary electrophoresis fluoroimmunoassay of blood PrpSc for evaluation of scrapie pathogenesis in sheep.     

David J Everest  Susan Waterhouse  Tina Kelly  Elena Velo-Rego  Maurice J Sauer 《Journal of veterinary diagnostic investigation》2007,19(5):552-557

Management of prion diseases in livestock would benefit greatly from availability of a validated blood test. A promising immunocapillary electrophoresis technique (also known as capillary electrophoresis fluoroimmunoassay) to detect abnormal prion protein in blood from live sheep is evaluated here. Capillary electrophoresis fluoroimmunoassay was applied to analysis of extracted blood from scrapie-exposed sheep (n = 87; 347 samples) at various stages of incubation, and to control sheep (n = 194; 489 samples). Overall, test values for the control and test populations were not significantly different, and a similar proportion of control (7%) and test (10%) sheep were classified as positive. Over 2-3 month intervals from birth until clinical disease, test specificity and sensitivity ranged from 66.7% to 100% and 0% to 66.7%, respectively, indicating poor diagnostic performance at all stages of pathogenesis. In routine application, in its present form, the capillary electrophoresis fluoroimmunoassay procedure proved to be insufficiently robust for use as a blood test for scrapie diagnosis.  相似文献   

Major gene resistance in Brassica napus (oilseed rape) is overcome by changes in virulence of populations of Leptosphaeria maculans in France and Australia     

Susan J. Sprague  Marie-Hélène Balesdent  Hortense Brun  Helen L. Hayden  Stephen J. Marcroft  Xavier Pinochet  Thierry Rouxel  Barbara J. Howlett 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):33-40

Resistance of Brassica napus (oilseed rape, canola) conferred by three different major resistance genes has been overcome by changes in virulence of Leptosphaeria maculans populations in France and Australia. In South Australia where B. napus cultivars with major gene resistance derived from Brassica rapa ssp. sylvestris were grown extensively, resistance was rendered ineffective within 3 years of commercial release of the cultivar. Disease severity was higher on cultivars with sylvestris-derived resistance than cultivars with polygenic resistance. This Australian situation is compared to that in France, where resistance conferred by the Rlm1 gene was overcome nation-wide in 5 years under commercial cropping practices, and also where a source of resistance introgressed into B. napus from B. juncea was rendered inefficient in 3 years in experimental field plots near Rennes.  相似文献   

The effects of cooling and vitrification of embryos from mares treated with equine follicle-stimulating hormone on pregnancy rates after nonsurgical transfer   总被引：1，自引：0，他引：1  

Jason Hudson DVM  Patrick M. McCue DVM  PhD  Elaine M. Carnevale DVM  PhD  Susan Welch MS  Edward L. Squires PhD 《Journal of Equine Veterinary Science》2006,26(2):51-54

Equine embryos can remain viable for 12 to 24 hours when cooled and stored at 5°C.¹ Cryopreservation of embryos would allow for long-term preservation of genetic material and more efficient management of embryo recipients. This study compared pregnancy rates after transfer of equine embryos vitrified within 1 hour of collection or cooled for 12 to 19 hours before vitrification. Mares (N = 40) were superovulated using equine follicle-stimulating hormone (eFSH). Embryos were recovered 6.5 days after ovulation or 8 days after human chorionic gonadotropin. Forty morulae or early blastocysts with a grade of 1 to 2 and <300 mm in diameter were randomly assigned to 1 of 2 treatments: Group 1 (n = 20), washed 4 times in a commercial holding medium and then vitrified; Group 2 (n = 20), washed 3 times and then stored in the same holding medium at 5°C to 8°C in a passive cooling device for 12 to 19 hours before being vitrified. To thaw, embryos were warmed by holding the straw in air at room temperature for 10 seconds and then submerged in a water bath (20°C to 22°C) for an additional 10 seconds. The contents of the straw were transferred directly into a recipient that had ovulated 4 to 6 days previously. There were no differences (P > .05) in embryo diameter, grade, or morphology score between treatment groups before vitrification. Pregnancy rates (day 16) were not different (P > .05) between embryos vitrified immediately after collection (15 of 20; 75%) and embryos cooled for 12 to 19 hours before vitrification (13 of 20; 65%). Based on these results, small equine embryos (<300 mm) can be stored at 5°C to 8°C for 12 to 19 hours before vitrification without a significant loss of viability.  相似文献   

A Combination Chemotherapy Protocol (VELCAP-L) for Dogs with Lymphoma   总被引：3，自引：1，他引：2  

Barbara I. Zemann  Antony S. Moore  William M. Rand  Gail Mason  David M. Ruslander  Angela E. Frimberger  Carrie A. Wood  Deborah A. L'Heureux  John Gliatto  Susan M. Cotter 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(6):465-470

Ninety-eight dogs with lymphoma treated with a 5-drug combination chemotherapy regimen (vincristine, L-asparaginase. cyclophosphamide, doxorubicin, prednisone [VELCAP-L]) were evaluated for pretreatment characteristics predictive for response and remission duration. The complete remission rate was 69%, with a median remission duration of 55 weeks. Dogs with advanced stage of disease, constitutional signs, dogs that were older, and dogs that were dyspneic were less likely to achieve remission. Once in remission, small dogs and dogs without pretreatment thrombocytopenia were likely to have longer remission duration. Toxicoses were frequent, but rarely fatal, and no predictitive factors were found for a dog developing toxicoses. VELCAP-L is an effective treatment for dogs in stage I-III lymphoma, particularly in young, small animals.  相似文献   

THE DURAL TAIL SIGN IN THE DIAGNOSIS OF MENINGIOMAS   总被引：3，自引：0，他引：3  

John P.  Graham  MVB  MSc  Susan M.  Newell  DVM  MS  Andra K.  Voges  DVM  Gregory D.  Roberts  DVM  MS  Jay M.  Harrison  MS 《Veterinary radiology & ultrasound》1998,39(4):297-302

In humans the dural tail is a sign seen on contrast enhanced T1 weighted magnetic resonance images. This finding is considered specific for meningioma. The purpose of this study was to determine how often the dural tail occurs in cats and dogs and whether it is a specific sign for meningiomas in these species. MR examinations for eighteen dogs and four cats with proven diagnoses were reviewed. Diagnoses included ten meningiomas (seven dogs and three cats), three gliomas, two pituitary tumors, single examples of two other tumor types and five patients with mass lesions due to inflammatory disease. Contrast enhanced T1 weighted images were evaluated independently by three of the authors for the presence of a dural tail, without knowledge of the diagnoses. The results were compared to the diagnosis for each patient and the performance of individual reviewers compared. When their results were averaged, the reviewers reported the presence of a dural tail in 6 of 10 (60%) meningiomas, although detection varied between observers from 40% to 80%. Each reviewer had one false positive result, two reported a dural tail with a chromophobe adenocarcinoma and one with a toxoplasma meningoencephalitis. When a dural tail is seen an associated mass is most likely a meningioma. It is uncertain whether the dural tail represents neoplastic infiltration beyond the margins of the meningioma. This should be considered when planning treatment.  相似文献   

Letters to the Editor ◆ Lettres À La Rédaction: New veterinary website          下载免费PDF全文

Susan Corning 《The Canadian veterinary journal. La revue veterinaire canadienne》1997,38(3):133

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Acute Reactions in Dogs Treated with Doxorubicin: Increased Frequency with the Use of a Generic Formulation     

Brenda S. Phillips  Susan A. Kraegel  Eric Simonson  Bruce R. Madewell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):171-172

During a 4-month period, 34 dogs with tumors received a total of 60 doses of a single generic formulation of doxorubicin; 13 acute drug reactions were observed in these 34 dogs, and no acute reactions were observed after replacing the product with the proprietary brand. These reactions were characterized by one or more of the following signs: pruritus; head-shaking; urticaria; erythema of the pinnal, axillary, or inguinal regions; vocalization; vomiting; hyperemic or pale mucous membranes; high heart rate; and high respiratory rate. We propose that a component unique to generic doxorubicin was responsible for the unusually high number of acute drug reactions observed.  相似文献   

Retrobulbar lidocaine injection via the supraorbital fossa is safe in adult horses but produces regionally variable periocular anaesthesia     

Vanessa Y. Yang  Joshua Seth Eaton  Katherine Harmelink  Scott J. Hetzel  Adriana Sanchez  Jane R. Lund  Lesley J. Smith 《Equine veterinary journal》2022,54(4):807-819

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The North American long-term soil productivity experiment: Findings from the first decade of research   总被引：1，自引：0，他引：1  

Robert F. Powers  D. Andrew Scott  Felipe G. Sanchez  Richard A. Voldseth  Deborah Page-Dumroese  John D. Elioff  Douglas M. Stone 《Forest Ecology and Management》2005,220(1-3):31-50

First decade findings on the impacts of organic matter removal and soil compaction are reported for the 26 oldest installations in the nation-wide network of long-term soil productivity sites. Complete removal of surface organic matter led to declines in soil C concentration to 20 cm depth and to reduced nutrient availability. The effect is attributed mainly to the loss of the forest floor. Soil C storage seemed undiminished, but could be explained by bulk density changes following disturbance and to decomposition inputs of organic C from roots remaining from the harvested forest. Biomass removal during harvesting had no influence on forest growth through 10 years. Soil compaction effects depended upon initial bulk density. Soils with densities greater than 1.4 Mg m⁻³ resisted compaction. Density recovery was slow, particularly on soils with frigid temperature regimes. Forest productivity response to soil compaction depended both on soil texture and the degree of understory competition. Production declined on compacted clay soils, increased on sands, and generally was unaffected if an understory was absent.  相似文献   

Factors influencing stump sprouting by pondcypress (Taxodium distichum var. nutans (Ait.) Sweet)     

Cotton?K.?RandallEmail author  Mary?L.?Duryea  Susan?W.?Vince  R.?Jeffery English 《New Forests》2005,29(3):245-260

Sprouting can be an important mechanism of regeneration in harvested cypress (Taxodium sp.) wetlands; however, limited information exists on factors influencing sprouting. We examined the influence of harvesting practices, tree size, and site conditions on stump sprouting in eight pondcypress (Taxodium distichum var. nutans (Ait.) Sweet) wetlands in Florida, USA. Stumps were cut at three heights: 20 cm below high water level, at high water, and 40 cm above high water. Half of each site was harvested using a sawhead feller and the other half using shearhead. We monitored sprouting for 2 years post-harvest. Stump height affected first year sprouting, but the effect disappeared during year 2. Felling equipment did not affect sprouting; however, the shearhead caused greater stump damage. Sprouting declined with increasing parent tree size; percentage of stumps with sprouts after 2 years was 68% for trees 10–14 cm dbh and 28% for trees 20–24 cm dbh. Stump sprouting ranged among sites from 23 to 54% after 2 years. Results indicate that sprouting may be an important, but inadequate, form of regeneration. At sites with short hydroperiods, dense understories, and/or predominance of trees greater than 20 cm dbh, reduced sprouting may occur, and planting seedlings may be beneficial.  相似文献