The JAK2/STAT5 signaling pathway as a potential therapeutic target in canine mastocytoma          下载免费PDF全文

Alexandra Keller  Bettina Wingelhofer  Barbara Peter  Karin Bauer  Daniela Berger  Susanne Gamperl  Martin Reifinger  Sabine Cerny‐Reiterer  Richard Moriggl  Michael Willmann  Peter Valent  Emir Hadzijusufovic 《Veterinary and comparative oncology》2018,16(1):55-68

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M-MODE ECHOCARDIOGRAPHIC REFERENCE VALUES IN CATS IN THE FIRST THREE MONTHS OF LIFE     

Susanne  Schille  Marianne  Skrodzki 《Veterinary radiology & ultrasound》1999,40(5):491-500

Thirty-five young cats were studied by echocardiography from the 2nd to 12th weeks of life to analyze correlation between body weight, body surface area, age and heart rate with fourteen echocardiographic parameters. There was a positive linear correlation (r = 0.49-0.78) between the independent variables (body weight, body surface area, age) and left ventricular wall thickness and diameter, aortic diameter and left atrial diameter, whereas there was a negative correlation (r = -0.39 and r = -0.43) between the heart rate and left ventricular diameter during systole and diastole. No linear dependence of the fractional shortening, ejection fraction, percentage thickening of the interventricular septum and left ventricular posterior wall, LA/AO ratio, and the ratio IVSED/LVWED to the independent variables was observed.  相似文献   

ULTRASONIC DIAGNOSIS OF OVARIAN CYSTS IN TEN GUINEA PIGS     

Attila  Beregi  DVM  Susanne  Zorn  Vet Med Student  Ferenc  Felkai  DVM PhD 《Veterinary radiology & ultrasound》1999,40(1):74-76

Ten guinea pigs with an ovarian cyst had clinical signs of anorexia, alopecia, or depression. Ultrasonographic features of the 2- to 3-cm diameter fluid-filled cysts included compartmentalization and connection to the ovary. In two animals, a unilateral cyst was present, and in two others, the cysts were bilateral. Diagnosis of the disease by plain radiography is difficult because of the similar opacity of ovarian cysts, abdominal neoplasms, and trichobezoars.  相似文献   

Comparative survival and growth performance of European lobster larvae,Homarus gammarus,reared on dry feed and conspecifics          下载免费PDF全文

Adam Powell  James Hinchcliffe  Kristina Sundell  Nils‐Gunnar Carlsson  Susanne P. Eriksson 《Aquaculture Research》2017,48(10):5300-5310

A bottleneck of crustacean larval culture concerns nutrition and associated cannibalism in communal rearing systems, which impact on larval survival, development and growth. For early‐stage European lobster, Homarus gammarus larvae, feeding ecology and body composition are largely unknown. We initiated four progressive feeding experiments (novel feed types, feeding regime and feed size and cannibalism effects) on growth and survival, to inform and update husbandry protocols. Performance of larvae offered a dry commercial feed was not significantly different compared with a conventional wet plankton feed of the same ration and size grade (both within 600–1,000 μm). Further experiments found that the same ration of dry feed offered six times daily improved development and growth, over the conventional regime of three times daily. Small‐grade dry feed (particles: 250–360 μm) improved larval performance compared with a larger feed (360–650 μm). Larvae were also fed different proportions of dry feed and/or conspecifics in both communal and individual rearing systems (the latter preventing cannibalism via segregation). Individually reared larvae, fed only dead conspecifics, displayed the greatest survival (80%) to postlarvae. This underlines the impact of cannibalism on survival and nutrition in H. gammarus larviculture. A final experiment analysed H. gammarus zoea 1 composition, identifying deficiencies in ash and carbohydrate in lobster feeds. This suggests a need for a species‐specific, formulated dry feed for H. gammarus larviculture. Our research represents the first investigation of H. gammarus larval composition and dietary requirements and highlights decreased growth potential associated with providing nutrition solely from generic commercial feed.  相似文献   

Indicative properties of fly‐ash affected forest soils in Northeastern Germany     

Susanne Klose  Juliane Koch  Ernst Bucker  Franz Makeschin 《植物养料与土壤学杂志》2001,164(5):561-568

Many forest ecosystems in Germany are strongly influenced by emissions of pollutants like SO₂ and alkaline dusts. To quantify and evaluate the consequences of long‐term fly ash deposition on forest soils, a study was conducted in pine stands (Pinus sylvestris) in the Dübener Heide in Northeastern Germany. This forest area has been influenced mainly by emissions from coal‐fired power plants and the chemical industry of the industrial region Bitterfeld‐Wolfen‐Zschornewitz since the early 1900. The study sites are located along a fly ash deposition gradient of 8, 16, 14, 18, and 25 km away from the main emission source in Bitterfeld (sites 1, 2, 3, 4, and 5, respectively). Samples of the organic horizons (Oi, Oe, and Oa) and mineral topsoil (0—10 cm) were taken in fall 1998 and analyzed for their ferromagnetic susceptibility and total ash content. Scanning electron microscopy (SEM) and energy dispersive X‐ray microanalysis (EDX) were performed on selected samples to differentiate between the pedogenic and atmospheric origin of the mineral components in the organic horizons. As a result of the long‐term deposition, ferromagnetic fly ash components are mainly accumulated in the Oe and Oa horizons of the forest soils studied. Ferromagnetic susceptibility was significantly higher (p ≤ 0.05) in the Oe horizon of sites 1 and 2 compared to sites 3, 4, and 5. Unusually high total ash contents for organic horizons of > 74 % were determined in the Oa at all sites. SEM revealed 3 distinct features of persistent fly ash deposits from coal‐fired power plants within the organic horizons that can be defined as ”︁stable glasses” with magnetic properties, aluminum‐silicate‐minerals, and slag fragments. SEM and EDX indicated that a great portion of the mineral particles found in the organic horizons of forests soils influenced by fly ash are from atmospheric sources. For detection of atmospheric lignite‐derived deposition into forest soils, the Oe and Oa horizons have to be considered as specific diagnostic horizons because they show indicative properties for such soils.  相似文献   

Effects of High and Low Molecular Weight Glutenin Subunits on Rheological Dough Properties and Breadmaking Quality of Wheat     

Susanne Antes  Herbert Wieser 《Cereal Chemistry》2001,78(2):157-159

High molecular weight (HMW) or low molecular weight (LMW) subunits of different chemical state (reduced, reoxidized with KBrO₃, or KIO₃) or gliadins were added in 1% amounts to a base flour of the wheat cultivar Rektor and mixed with water. The corresponding doughs were then characterized by microscale extension tests and by microbaking tests and were compared to doughs from the base flour without additives. The maximum resistance of dough was strongly increased by HMW subunits in a reduced state and by HMW subunits reoxidized with KBrO₃. A moderate increase of resistance was caused by HMW subunits reoxidized with KIO₃ and by LMW subunits reoxidized with KBrO₃ or KIO₃. This resistance was strongly lowered by LMW subunits in a reduced state and by gliadins. The extensibility of dough was significantly increased only by gliadins and reduced HMW subunits; HMW subunits reoxidized with KBrO₃ had no effect, and all other fractions had a decreasing effect. In particular, glutenin subunits reoxidized with KIO₃ induced marked decrease of extensibility, resulting in bell‐shaped curve extensigrams, which are typical for plastic properties. The effect of reoxidized mixtures (2:1) of HMW and LMW subunits on maximum resistance depended on the oxidizing agent and on the conditions (reoxidation separated or together); extensibility was generally decreased. Bread volume was increased by addition of HMW subunits (reduced or reoxidized with KBrO₃) and decreased by LMW subunits (reoxidized with KBrO₃ or KIO₃) and by a HMW‐LMW subunit mixture (reoxidized with KBrO₃). The volume was strongly decreased by addition of reduced LMW subunits. A high bread volume was related to higher values for both resistance and extensibility.  相似文献   

Comparative survival and growth performance of European lobster Homarus gammarus post‐larva reared on novel feeds     

James Hinchcliffe  Adam Powell  Markus Langeland  Aleksandar Vidakovic  Ingrid Undeland  Kristina Sundell  Susanne P. Eriksson 《Aquaculture Research》2020,51(1):102-113

One approach to ongrow juvenile European lobster, Homarus gammarus, is to utilize land based rearing systems, incorporating automated feeding, individual culture and provision of stable pelleted feeds, preferably using sustainable ingredients. We initiated three feeding experiments to investigate the general suitability of ingredients produced from seafood by‐products as novel feeds for H. gammarus, in terms of promoting survival, development and growth of post‐larval lobsters from post‐larvae (PL) stage IV to the first juvenile stage (stage V). The first experiment was designed to screen an array of candidate, locally produced, novel protein sources on growth performance parameters. This initial experiment revealed that PL reared on a raw (i.e. wet, unprocessed shrimp) feed used as a reference showed superior performance to those reared on experimental feeds containing fishmeal, herring protein isolate or mussel meal; however, a novel type of shrimp meal, produced by flocculation from waste water, promoted the best PL performance of any experimental feed. A second experiment was designed to test the effect of drying method and to optimize the form of a wet shrimp reference feed used by lobster hatcheries. This showed that the performance of PL reared on experimental freeze‐dried shrimp feed was not significantly different to those reared on the wet, unprocessed shrimp used as a reference feed. However, lobsters offered experimental oven‐dried shrimp feed (with or without an immune supplement) resulted in significantly lower survival or growth performance. A third and final experiment was designed in an attempt to improve a candidate herring‐based protein source, by supplementing with nutrients found in shrimp. However, the results showed that PL reared on the wet reference shrimp feed still showed superior growth and survival than those reared on a herring feed alone, or supplemented with additives found in shrimp meal (either glucosamine, astaxanthin or both supplements combined). The high survival and growth, low incidence of moulting problems and high availability of waste shrimp material, suggest that non‐heat‐treated shrimp products are a promising feed ingredient for post‐larval European lobsters.  相似文献   

Mobile and readily available C and N fractions and their relationship to microbial biomass and selected enzyme activities in a sandy soil under different management systems     

Dirk Landgraf  Susanne Klose 《植物养料与土壤学杂志》2002,165(1):9-16

Within different land‐use systems such as agriculture, forestry, and fallow, the different morphology and physiology of the plants, together with their specific management, lead to a system‐typical set of ecological conditions in the soil. The response of total, mobile, and easily available C and N fractions, microbial biomass, and enzyme activities involved in C and N cycling to different soil management was investigated in a sandy soil at a field study at Riesa, Northeastern Germany. The management systems included agricultural management (AM), succession fallow (SF), and forest management (FM). Samples of the mineral soil (0—5, 5—10, and 10—30 cm) were taken in spring 1999 and analyzed for their contents on organic C, total N, NH₄⁺‐N and NO₃^—‐N, KCl‐extractable organic C and N fractions (Corg_(KCl) and Norg_(KCl)), microbial biomass C and N, and activities of β‐glucosidase and L‐asparaginase. With the exception of Norg_(KCl), all investigated C and N pools showed a clear relationship to the land‐use system that was most pronounced in the 0—5 cm profile increment. SF resulted in greater contents of readily available C (Corg_(KCl)), NH₄⁺‐N, microbial biomass C and N, and enzyme activities in the uppermost 5 cm of the soil compared to all other systems studied. These differences were significant at P ≤ 0.05 to P ≤ 0.001. Comparably high C_mic:C_org ratios of 2.4 to 3.9 % in the SF plot imply a faster C and N turnover than in AM and FM plots. Forest management led to 1.5‐ to 2‐fold larger organic C contents compared to SF and AM plots, respectively. High organic C contents were coupled with low microbial biomass C (78 μg g^—1) and N contents (10.7 μg g^—1), extremely low C_mic : C_org ratios (0.2—0.6 %) and low β‐glucosidase (81 μg PN g^—1 h^—1) and L‐asparaginase (7.3 μg NH₄‐N g^—1 2 h^—1) activities. These results indicate a severe inhibition of mineralization processes in soils under locust stands. Under agricultural management, chemical and biological parameters expressed medium values with exception for NO₃^—‐N contents which were significantly higher than in SF and FM plots (P ≤ 0.005) and increased with increasing soil depth. Nevertheless, the depth gradient found for all studied parameters was most pronounced in soils under SF. Microbial biomass C and N were correlated to β‐glucosidase and L‐asparaginase activity (r ≥ 0.63; P ≤ 0.001). Furthermore, microbial biomass and enzyme activities were related to the amounts of readily mineralizable organic C (i.e. Corg_(KCl)) with r ≥ 0.41 (P ≤ 0.01), suggesting that (1) KCl‐extractable organic C compounds from field‐fresh prepared soils represent an important C source for soil microbial populations, and (2) that microbial biomass is an important source for enzymes in soil. The Norg_(KCl) pool is not necessarily related to the size of microbial biomass C and N and enzyme activities in soil.<?show $6#>  相似文献   

Levels of Avenanthramides and Activity of Hydroxycinnamoyl‐CoA:Hydroxyanthranilate N‐Hydroxycinnamoyl Transferase (HHT) in Steeped or Germinated Oat Samples     

Susanne Bryngelsson  Atsushi Ishihara  Lena H. Dimberg 《Cereal Chemistry》2003,80(3):356-360

Concentrations of avenanthramides and activity of the biosynthetic enzyme hydroxycinnamoyl‐Co A:hydroxyanthranilate N‐hydroxycinnamoyl transferase (HHT) were analyzed in dry or, steeped nonmilled or milled, non‐heat‐treated (raw) or heat‐treated oat samples (Avena sativa L.). Increased avenanthramide concentrations were found when intact raw groats were steeped. The increase was time‐ and temperaturedependent and maximal after 10 hr of steeping at 20°C. Continuous germination in air, after steeping, only contributed to a further increase in avenanthramides when steeping times were shorter than 10 hr. Concentrations of avenanthramides and HHT activity were positively correlated during steeping of intact groats at 8 and 20°C. The increase in avenanthramides was suggested to be due to de novo synthesis and a whole grain structure seemed to be required as no increase was found when groats were milled before steeping. Avenanthramide levels also increased when heat‐treated samples, lacking HHT activity, were steeped. This increase may be due to release of bound forms, possibly formed during the preceding heat treatment.  相似文献   

Presence of Muscarinic Acetylcholine Receptors in the Cattle Tick Boophilus microplus and in Epithelial Tissue Culture Cells of Chironomus tentans     

Andreas Turberg  Iris Schrder  Susanne Wegener  Michael Londershausen 《Pest management science》1996,48(4):389-398

A muscarinic acetylcholine receptor (mAChR) has been demonstrated and partially characterized in larvae of the cattle tick Boophilus microplus. Its properties are compared with mAChR from an epithelial cell line from the dipteran insect Chironomus tentans. Competition studies with cholinergic ligands of different specificity revealed the muscarinic nature of the cholinergic receptors investigated in both species. In homogenates from tick larvae, specific binding sites for [³H]quinuclidinyl benzilate (QNB) with high affinity (1·2±(0·13) nM ; B_max 22·5 pmol mg protein⁻¹) were detected that do not bind nicotinic compounds specifically. The estimated IC₅₀ values for nicotine, imidacloprid and α-bungarotoxin were all in the mM range. Additionally, with tick larvae, high-affinity nicotinic binding sites were detected with [³H]nicotine which could be displaced by high concentrations of imidacloprid or QNB. The estimated IC₅₀ values for nicotine, α-bungarotoxin, imidacloprid and QNB were 43(±8) nM , 0·8(±0·2) μM , 2·8(±0·6) μM and 78(±1·9) μM , respectively. With homogenates of the non-neuronal insect cell line from C. tentans, only high-affinity binding sites for [³H]QNB were found. Muscarinic antagonists selectively displaced [³H]quinuclidinyl benzilate (QNB) binding to tick larvae homogenates. The mAChR of B. microplus preferred pirenzepine (IC₅₀ 2·13(±1·02) μM ) among different subtype-specific mAChR antagonists (4-DAMP had IC₅₀ 49·9(±9·13) μM and methoctramine had IC₅₀ 121(±14·2) μM ) indicating a type of binding site similar to the vertebrate M1 mAChR subtype. The tick muscarinic receptor seems to be a G-protein-coupled receptor, as concluded from the 4·8-fold reduction in receptor affinity for binding of the muscarinic agonist oxotremorine M upon treatment with the non-hydrolysable GTP-analogue γ-S-GTP. Binding data for the agonists oxotremorine M (IC₅₀ 71·3(±19·6) μM ) and carbachol (IC₅₀ 253(±87·1) μM ) parallel the biological efficacy of these compounds, in that, while oxotremorine M showed some activity against ticks, carbachol was ineffective.  相似文献