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81.
K F Bowman C L Sweeney L P Tate 《Journal of the American Veterinary Medical Association》1987,190(3):305-307
A medial condylar fracture of the third metatarsal bone in a 2-year-old Thoroughbred filly was repaired successfully by use of compression bone plating. At discharge from the hospital, thickening of the metatarsus was evident, and proliferative changes were associated with the site of bone plate application. It was expected that these changes would limit the filly's use as a racehorse, and a return to training was not recommended. Especially in horses that may return to racing, use of compression bone plating initially should not replace stall rest or lag screw fixation treatment of this type of injury. However, the use of a similar approach in selected cases of medial condylar fracture of the third metatarsal bone is encouraged, especially when additional fissure lines are evident on radiographs. 相似文献
82.
Davis R Jayappa H Abdelmagid OY Armstrong R Sweeney D Lehr C 《Veterinary therapeutics : research in applied veterinary medicine》2007,8(1):32-40
Healthy dogs with low antibody titer to Bordetella bronchiseptica were vaccinated intranasally with an avirulent live vaccine, subcutaneously with an antigen extract vaccine, or subcutaneously and intranasally with a placebo. Intranasally vaccinated dogs developed B. bronchiseptica-specific IgA titers in nasal secretions that remained at high levels until the end of the study; dogs vaccinated subcutaneously with the antigen extract or placebo did not develop measurable antigen-specific IgA titers in nasal secretions. Dogs were challenged with virulent live B. bronchiseptica 63 days after vaccination. Intranasally vaccinated dogs had significantly lower cough scores (P < or =.0058) and shed significantly fewer challenge organisms (P <.0001) than dogs in either of the other groups. Cough scores of subcutaneously vaccinated dogs were not significantly different from placebo-vaccinated dogs. 相似文献
83.
Hines ME Stabel JR Sweeney RW Griffin F Talaat AM Bakker D Benedictus G Davis WC de Lisle GW Gardner IA Juste RA Kapur V Koets A McNair J Pruitt G Whitlock RH 《Veterinary microbiology》2007,122(3-4):197-222
An international committee of Johne's disease (JD) researchers was convened to develop guidelines for JD challenge studies in multiple animal species. The intent was to develop and propose international standard guidelines for models based on animal species that would gain acceptance worldwide. Parameters essential for the development of long-term and short-term infection models were outlined and harmonized to provide a "best fit" JD challenge model for cattle, goats, sheep, cervids, and mice. These models will be useful to study host-pathogen interactions, host immunity at the local and systemic level, and for evaluating vaccine candidates and therapeutics. The consensus guidelines herein list by animal species strains of Mycobacterium avium subsp. paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters. 相似文献
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Free-living rhizobia are sensitive to soils and artificial media that are acidic. Both excessive H+ and Al released from acid-soluble minerals appear to be toxic. The complex, heterotrophic nutrient requirements of rhizobia and the joint occurrence of Al3+ and hydroxo-, sulphato-, phosphato-, fluoro-, and other Al species have prevented a precise attribution of toxicity to the Al species. In the present study, a medium composed of 0.3 mM MgSO4, 2 mM CaCl2, and 10 mM sucrose (the basal medium) enabled a 1000-fold cell increase at pH 4.6 or above. Additions of 1 μM AlCl3 to the basal medium were highly intoxicating, especially at higher pH: below pH 5.0 cell numbers increased slightly; at pH 5.0 cell numbers did not change from the inoculum; at higher pH values the cell numbers declined. Similar trends were observed for La3+ and Cu2+ intoxication. Uptake of methylene blue, a positively charged dye useful as a probe of cell-surface electrical potential, was inhibited by pH reductions between pH 3.5 and 6.0. Factors that decrease cell-surface negativity (such as lower pH) reduce the intoxication by cations in plant roots, but the pH responsiveness of the rhizobia in our system was much greater than the pH responsiveness of plants. Although plant-root intoxication by mononuclear hydroxo-Al species has been discounted, rhizobia may be sensitive to those species. These results have implications for the management of rhizobia in acidic soils and for the development of resistant strains. 相似文献
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Asfaw Adugna Allison A. Snow Patty M. Sweeney Endashaw Bekele Evans Mutegi 《Genetic Resources and Crop Evolution》2013,60(4):1313-1328
In situ population studies of wild relatives of crops are crucial for the conservation of plant genetic resources, especially in regions with high genetic diversity and a risk of local extinction. Ethiopia is the center of origin for sorghum, yet little is known about the genetic structure of extant wild populations. Using 9 Simple Sequence Repeat loci, we characterized 19 wild populations from five regions, 8 local cultivar populations from three regions, and 10 wild sorghum accessions from several African countries. To our knowledge, this is the most comprehensive study to date of in situ wild sorghum populations in Africa. Genetic diversity corrected for sample size was significantly greater in the wild populations in situ than in local cultivars or the accessions. Approximately 41 % of the genetic variation in the wild plants was partitioned among populations, indicating a high degree of differentiation and potential value for germplasm conservation, and the average number of migrants (Nm) per generation was 0.43. Cluster analyses showed that some wild populations were grouped by geographic region, whereas others were not, presumably due to long-distance seed movement. Four wild populations from disjunct regions formed a unique cluster with an Ethiopian accession of subsp. drummondii and probably represent a weedy race. STRUCTURE and other analyses detected evidence for crop-wild hybridization, consistent with previous molecular marker studies in Kenya, Mali, and Cameroon. In summary, in situ wild sorghum populations in Ethiopia harbor substantial genetic diversity and differentiation, despite their close proximity to conspecific cultivars in this crop/wild/weedy complex. 相似文献
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Intra‐ and interobserver reliability estimates for identification and grading of upper respiratory tract abnormalities recorded in horses at rest and during overground endoscopy
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C. L. McGivney J. Sweeney F. David J. M. O'Leary E. W. Hill L. M. Katz 《Equine veterinary journal》2017,49(4):433-437