Growth response of field-grown Siratro (Macroptilium atropurpureum Urb.) and Aeschynomene american L. to inoculation with selected vesicular-arbuscular mycorrhizal fungi     

O. A. Medina  A. E. Kretschmer Jr.  D. M. Sylvia 《Biology and Fertility of Soils》1990,9(1):54-60

Summary The effect of inoculation with a selected isolate of Glomus etunicatum Becker and Gerdemann and one of G. intraradices Schenck and Smith on the growth and nutrient content of Macroptilium atropurpureum Urb. cv. Siratro and Aeschynomene americana L., at applied P levels of 10, 30, 60, and 120 kg ha^-1, was studied under field conditions. At all P levels and for all harvests, the shoot dry mass of Siratro and A. americana were greater for the plants inoculated with the vesicular-arbuscular mycorrhizal (VAM) fungi than the control plants. Differences between the VAM fungus-inoculated and the control plants were most marked between 30 and 90 kg ha^-1 of applied P and diminished at 120 kg ha^-1. At the first harvest of Siratro, the plants inoculated with G. etunicatum had a greater shoot dry mass than those inoculated with G. intraradices, for all levels of applied P. However, for subsequent harvest of Siratro and for the one harvest of A. americana the response of shoot dry mass to the two VAM fungi was equivocal. Fungal inoculation gave at least a 30% saving in the amount of P fertilizer required (40 kg ha^-1) for the maximum yield. The plants inoculated with VAM fungi had a greater tissue concentration and total content of P and N than the control plants at low and intermediate levels of applied P. The percentage of root colonized by VAM fungi for the inoculated plants of the two legumes increased linearly with P additions up to 60 kg ha^-1. The conclusion is that under amended (limed and fertilized) soil conditions, inoculation with selected VAM fungi can improve the establishement and growth of forage legumes in fields that contain ineffective populations of native VAM fungi.  相似文献   

Assessing the impact of interfering organic matter on soil metaproteomic workflow     

Matthias Waibel  Kevin McDonnell  Maria Tuohy  Sally Shirran  Sylvia Synowsky  Barry Thornton  Eric Paterson  Fiona Brennan  Florence Abram 《European Journal of Soil Science》2023,74(3):e13392

Soil organic matter (SOM) is biologically, chemically, and physically complex. As a major store of nutrients within the soil, it plays an important role in nutrient provision to plants. An enhanced understanding of SOM utilisation processes could underpin better fertiliser management for plant growth, with reduced environmental losses. Metaproteomics can allow the characterisation of protein profiles and could help gain insights into SOM microbial decomposition mechanisms. Here, we applied three different extraction methods to two soil types to recover SOM with different characteristics. Specifically, water-extractable organic matter, mineral-associated organic matter and protein-bound organic matter were targeted with the aim to investigate the metaproteome enriched in those extractions. As a proof-of-concept, replicated extracts from one soil were further analysed for peptide identification using liquid chromatography followed by tandem mass spectrometry. We employed a framework for mining mass spectra for both peptide assignment and fragmentation pattern characterisation. Different extracts were found to exhibit contrasting total protein and humic substance content for the two soils investigated. Overall, water extracts displayed the lowest humic substance content (in both soils) and the highest number of peptide identifications (in the soil investigated) with the most frequent peptide hits associated with diverse substrate/ligand binding proteins of Proteobacteria and derived taxa. Our framework also highlighted a strong peptidic signal in unassigned and unmatched spectra, information that is currently not captured by the pipelines employed in this study. Taken together, this work points to specific areas for optimisation in chromatography and mass spectrometry to adequately characterise SOM-associated metaproteomes.  相似文献   

A case in Europe of feline histoplasmosis apparently limited to the skin     

Nina M. Fischer  Claude Favrot  Michel Monod  Paula Grest  Katia Rech  Sylvia Wilhelm 《Veterinary dermatology》2013,24(6):635-e158

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Extraction, processing, and storage effects on curcuminoids and oleoresin yields from Curcuma longa L. grown in Jamaica     

Green CE  Hibbert SL  Bailey-Shaw YA  Williams LA  Mitchell S  Garraway E 《Journal of agricultural and food chemistry》2008,56(10):3664-3670

Aromatic diarylheptanoid compounds from Curcuma longa Linn grown in Jamaica were quantified by UV-vis spectrophotometry and high-performance liquid chromatographic (HPLC) analyses. The oleoresin yields from ethanolic extracts were quantified and evaluated with regard to the effects of the type of postharvesting process and the type of extraction method conducted on the plant material. Fresh samples that were hot solvent extracted provided the highest oleoresin yields of 15.7% +/- 0.4 ( n = 3), and the lowest oleoresin yields of 7.8% +/- 0.2 ( n = 3) were from the dried milled samples that were cold solvent extracted. Data from the ASTA spectrophotometer assay confirmed that dried samples contained the highest curcuminoid content of 55.5% +/- 2.2 ( n = 6) at the fifth month of storage, and the fresh samples showed a curcuminoid content of 47.1% +/- 6.4 ( n = 6) at the third month of storage. A modified HPLC analysis was used to quantify curcumin content. Data from the HPLC analysis confirmed that the dried treated, hot extracted, room temperature stored samples had the highest curcumin content of 24.3%. A novel high-performance thin layer chromatography (HPTLC) method provided a chemical fingerprint of the C. longa with the use of a commercial curcumin standard.  相似文献   

Animal behavior case of the month. Persistent vomiting of food and gastric fluid about 3 times a day for the previous 24 months     

García-Belenguer S  Rosado B  Villegas A  Palacio J 《Journal of the American Veterinary Medical Association》2008,232(6):845-846

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Die Verdaulichkeit der Rückstände der Kürbisölbereitung und ihr Gehalt an Mineralstoffen     

Wilhelm Liebscher 《Journal of animal physiology and animal nutrition》1943,6(2):97-106

Verdauungsversuche ergaben, daß die schalenarmen Rückstände der Kürbisölbereitung eiweißreiche und hochverdauliche Futtermittel sind. Die Verdaulichkeit der organischen Substanz betrug 82,8%. Mit zunehmendem Schalenanteil sinkt die Verdaulichkeit aller Bestandteile der organischen Substanz. Sie betrug bei einem schätzungsweisen Gehalt von rund 50% Schalen 63,5 % und bei den reinen Schalen nur mehr 21,5 %. Auf die Wichtigkeit einer Rohfaserbestimmung bei der Untersuchung und Beurteilung der hierher gehörigen Rückstände wird hingewiesen. Die aus den durchgeführten Mineralstoffanalysen errechneten Äquivalente ergaben für den Kuchen und den Schrot einen Säure-, für die Schalen einen Basenüberschuß.  相似文献   

Responses of Australian sea lions,Neophoca cinerea,to anthropogenic activities in the Perth metropolitan area,Western Australia          下载免费PDF全文

Sylvia K. Osterrieder  Chandra Salgado Kent  Randall W. Robinson 《水产资源保护:海洋与淡水生态系统》2017,27(2):414-435

Einfluss eisenhaltiger Klärschlämme auf Kenngrößen der P‐Verfügbarkeit in Ackerböden     

Wilhelm Rmer  Ihab F. Samie 《植物养料与土壤学杂志》2001,164(3):321-328

Influence of iron content in sewage sludges on parameters of phosphate availability in arable soils The use of iron salts for the P elimination in sewage plants is widely used. But it is not clear whether the P availability in arable soils is negatively influenced by iron compounds or not. The aim of the investigations was, therefore, to study the influence of two sewage sludges with a high and a low Fe content respectively on P sorption and phosphate concentration (P_i) in the soil solution after application of CaHPO₄ or sewage sludge to 5 loamy and 4 sandy soils (pot experiments and 1 silty loam (field experiment)). Soils were analyzed 1, 6, and 13 months after P application. Sludge Gö contained 12 kg P and 65 kg Fe (t DM)^—1 (P : Fe = 1 : 5.4) and sludge Sh 25 kg P and 39 kg Fe (t DM)^—1 (P : Fe = 1 : 1.5). The basic P application was 60 kg P ha^—1 (= 30 mg P (kg soil)^—1 in the pot experiment, as sludge or as CaHPO₄). P uptake by maize was determined in a separate pot experiment with a loamy soil and the same P application rate. The P sorption capacity remained similar in all soils after application of sludge Sh (P : Fe = 1:1.5) compared with soils without sludge, however, after application of sludge Gö the P sorption increased by 16% (0—59%). After application of sludge Sh the mean P_i concentration increased in loamy soils by 34% and in sandy soils by 15%. On the other hand the P_i concentration decreased after applying sludge Gö by 13% and 36% as compared to the controls of the respective soils. In the field experiment the P_i concentration of plots with a high P level (50 mg lactate soluble P (kg soil)^—1) was also significantly decreased after application of 10 t sludge Gö (126 kg P ha^—1) in comparison with triple phosphate. One month after the application of increasing amounts of sludge Gö (5, 10, 15 t DM ha^—1) both the concentration of oxalate‐soluble Fe in the soil and the P sorption were increased. The elevated relationship between these two parameters was highly significant (r² = 0.6 — 0.97). Plant uptake of P was less after application of sludge Gö than after application of sludge Sh and much less than P uptake from CaHPO₄. Sewage sludges with a P : Fe ratio of 1 : 5 should not be recommended for agricultural use, as the P availability is significantly reduced. Iron salts should not be used for conditioning of sludges.  相似文献   

The acquisition of cadmium by Lupinus albus L., Lupinus angustifolius L., and Lolium multiflorum Lam.     

Wilhelm Rmer  Dong‐Kyu Kang  Komi Egle  Jrg Gerke  Holger Keller 《植物养料与土壤学杂志》2000,163(6):623-628

Das Cadmiumaneignungsvermögen von Lupinus albus L., Lupinus angustifolius L. und Lolium multiflorum Lam. Mehrere Pflanzenarten mobilisieren Bodenphosphate (P) und Kationen wie Fe und Al durch die Exsudation organischer Anionen und Protonen. Deshalb untersuchten wir das Cd‐Aneignungsver‐mögen von P‐, Fe‐, Al‐mobilisierenden Arten (Lupinus albus L., Lupinus angustifolius L.) im Vergleich zu einer nicht mobili‐sierenden Pflanzenart (Lolium multiflorum Lam.). Die Pflanzen wuchsen in zwei stark unterschiedlichen Böden (saurer Humuspodsol, karbonathaltiger Lössunterboden). Die Cd‐Aufnahme in die Sprosse war bei Weidelgras 5 bis 10 mal höher als bei Blauen bzw. Weißen Lupinen. Dieses Ergebnis hat mehrere Ursachen: 1. Das Wurzellängen/Sprossmasseverhältnis des Weidelgrases ist 2—3 mal größer als das der Lupinenpflanzen. 2. Bei Weidelgras wird ein größerer Teil des aufgenommenen Cd in die Sprosse verlagert. 3. Die Cd‐Aufnahme bei Lupinen ist im sauren Boden (Podsol) und bei P‐Mangel auch im Kalkboden niedriger als bei Weidelgras. Während im Podsol die Cd‐Konzentration der Bodenlösung unter Lupine geringer war als in der Kontrolle (Gefäße ohne Pflanzen), war sie im Kalkboden höher. Bei den Lupinen war der Efflux organischer Säureanionen, vor allem Citrat und Malat, um den Faktor 10—100 höher als bei Weidelgras. Diese Exsudation kann zu einer hohen Cd‐Komplexierung, insbesondere durch Citrat, in der Rhizosphärenbodenlösung führen (˜ 85%). Diese Ergebenisse deuten darauf hin, dass das komplexierte Cd von den Wurzeln schlechter aufgenommen wird als das freie Cd.  相似文献   

Eumycetoma and disseminated phaeohyphomycosis in a Sumatran tiger     

Amanda R. Schaff  Sylvia Ferguson  Kristen Phair  Rachel Ferris  Alexandra Goe 《Journal of veterinary diagnostic investigation》2021,33(6):1197

A 16-y-old female Sumatran tiger (Panthera tigris sumatrae) was evaluated for hyporexia. Examination revealed chronic kidney disease and a large subcutaneous axillary mass with draining tracts that contained numerous small black grains. Histologic examination revealed the presence of intralesional fungal hyphae. Persistent hyporexia and pyogranulomatous disease, as well as progressive cachexia and azotemia occurred despite treatment, and euthanasia was performed. Disseminated phaeohyphomycosis was diagnosed on postmortem examination, additionally affecting various lymph nodes, the nasal cavity, mesenteric adipose tissue, abdominal aorta, pericardium, and kidney. Fungal culture from a deep-tissue sample isolated a pure growth of Curvularia sp., a dematiaceous opportunistic fungus able to cause eumycetomas and/or phaeohyphomycosis. Phaeohyphomycosis is a rare but emerging condition, not previously reported as disseminated disease in an exotic carnivore, to our knowledge. Aggressive systemic antifungal treatment was unsuccessful, likely complicated by diagnostic challenges and concurrent renal disease. The presence of a swelling with abundant grains exiting draining tracts should direct clinicians to the diagnosis of a mycetoma, warranting early and aggressive treatment.  相似文献