三种食用百合种质资源限制生长保存及遗传稳定性研究     

万珠珠  牛来春  谭秀梅  刘敏  吴亮  董草 《北方园艺》2016,(13):89-92

以3种食用百合试管苗为试材,采用限制生长保存法,研究了不同浓度生长抑制剂对食用百合试管苗保存效果的影响,并对保存后的试管苗进行遗传稳定性检测,以期建立食用百合种质资源离体保存体系。结果表明:龙芽百合试管苗用10mg·L~(-1)防落素(PCPA)处理保存300d后,试管苗生长缓慢,存活率达96.00%;川百合试管苗用2.0mg·L~(-1)青鲜素(MH)处理保存150d后,试管苗的抑制生长效果明显,结鳞率和存活率高达100.00%;兰州百合试管苗用10mg·L~(-1)多效唑(PP333)处理保存300d后,试管苗抑制作用明显,结鳞率达100.00%,存活率达94.40%。比较限制生长保存后与未保存植株的可溶性蛋白和酯酶同工酶图谱,各处理和对照图谱带相似,初步证明了以上保存方法的可行性,较好的保持了遗传稳定性。  相似文献   

不同低温春化天数和赤霉素处理对解除洋桔梗莲座化的影响     

万珠珠  谭秀梅  牛来春  刘敏  吴亮  董草 《北方园艺》2016,(14):62-65

以2个洋桔梗品种"雪莱香槟"(‘Ceremony Orange’)和"露西塔I白底粉边"(‘Rosita I Pink Picotee’)为试材,研究了育苗期种子低温(10℃)春化处理天数(35、28、21、14、0d)及栽培期赤霉素处理对解除洋桔梗莲座化的影响,探寻了生产上解除洋桔梗莲座化技术。结果表明:2个品种低温春化处理28d均能提高种子发芽率。定植60d后,低温春化处理35d洋桔梗‘Ceremony Orange’抽薹率最高,达72.9%;低温春化处理28d‘Rosita I Pink Picotee’抽薹率最高,达89.6%。栽培期对未经种子低温春化处理的洋桔梗‘Ceremony Orange’莲座化苗进行赤霉素处理(处理浓度为0、50、100、150mg·L~(-1)),100mg·L~(-1)赤霉素处理对促进洋桔梗节间的伸长有明显作用,抽薹率达70.5%,可以有效打破莲座化。比较2种解除莲座化的处理效果,生产上解除洋桔梗莲座化方法可首选种子低温春化处理,该方法上花时间比栽培期赤霉素处理提早2个多月;栽培期赤霉素处理可以作为种子低温春化未打破莲座化的辅助措施。  相似文献   

授粉技术和单株留瓜数对鄂丝瓜1号制种效果的影响     

黄树苹  谈杰  张敏  王春丽  陈霞  徐长城  谈太明 《长江蔬菜》2016,(8)

以鄂丝瓜1号的父、母本为试材,以父本当天上午开放的雄花为花粉来源,研究了授粉时间、花粉供给量和单株留瓜数对种子产量和质量的影响。试验结果表明,母本雌花开放的当天上午和开花前1 d上午均可作为鄂丝瓜1号制种的适宜授粉时间;以1朵雄花为3朵雌花进行授粉为鄂丝瓜1号制种的首选花粉供给量;鄂丝瓜1号制种中,1株母本可以留5~6个种瓜。  相似文献   

Seroprevalence of and agroecological risk factors for Mycobacterium avium subspecies paratuberculosis and neospora caninum infection among adult beef cattle in cow-calf herds in Alberta, Canada     

Scott HM  Sorensen O  Wu JT  Chow EY  Manninen K 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(4):397-406

A province-wide cross-sectional seroprevalence and agroecological risk factor study of Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) infection among cattle in 100 cow-calf herds in Alberta was conducted. The seroprevalence of MAP in adult cattle was 1.5% across all herds. Using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (CI): 2.3-23.4%). Seroprevalence of MAP differed by agroecological region; specifically, cattle and herds in areas with high soil pH (> 7.0), southern latitudes, and arid climates had a moderately reduced risk of infection (P < 0.10). Seroprevalence of NC infection was 9.7% among adult beef cattle province-wide--these levels also varied by agroecological region--with 91.0% of herds infected overall.  相似文献   

Severe nonpurulent encephalitis with mortality and feather lesions in call ducks (Anas platyrhyncha var. domestica) inoculated intravenously with H5N1 highly pathogenic avian influenza virus     

Yamamoto Y  Nakamura K  Kitagawa K  Ikenaga N  Yamada M  Mase M  Narita M 《Avian diseases》2007,51(1):52-57

One-day-old, 2-wk-old, and 4-wk-old call ducks (Anas platyrhyncha var. domestica) inoculated intravenously with the H5N1 highly pathogenic avian influenza virus A/chicken/Yamaguchi/7/2004 isolate (Ck/Yama/7/04) were examined clinically, pathologically, and virologically. Clinically, the birds exhibited mild-to-severe neurologic signs and corneal opacity. All birds in the 1-day-old group and one bird in the 4-wk-old group died within 4 days after the virus inoculation. Histologic changes were characterized by severe nonpurulent encephalitis and necrotic lesions of feather epithelium on day 3 postinoculation (PI) or later. Focal necrosis of myocardial cells, pancreatic acinar cells, skeletal myocytes, and corneal epithelial cells was observed. Viral antigens were detected in association with necrotic changes. Viruses were isolated from all examined organs including the skin with many feathers. Serum antibody against the virus was detected in all surviving birds on day 10 PI by hemagglutination-inhibition tests. These results suggest that Ck/Yama/7/04 has a pathogenicity that causes neurologic sign, nonpurulent encephalitis with mortality, and feather lesions for call ducks. Feather lesions with viral antigens and the virus isolation from the skin suggest that Ck/Yama/ 7/04 has a predilection for feathers in call ducks.  相似文献   

Changes in the mRNA transcripts of insulin-like growth factor ligand, receptors and binding proteins in bovine blastocysts and elongated embryos derived from somatic cell nuclear transfer     

Sawai K  Kageyama S  Moriyasu S  Hirayama H  Minamihashi A  Onoe S 《The Journal of reproduction and development》2007,53(1):77-86

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励磁功率单元主回路快速故障诊断策略     

刘伟  张朕滔  梁康有  谭宇航 《保鲜与加工》2015,(4):137-145

分析总结了发电机组励磁系统功率单元的故障类型和常见故障,通过Matlab仿真功率单元主回路故障类型,重点研究基于故障频谱分析的定量故障特征提取和故障分类,提出了一种快速针对功率单元主回路的故障诊断真值表法,最后通过实验平台验证故障类别,结果表明：该方法能够正确有效地诊断出功率单元主回路具体的故障晶闸管,为励磁系统功率单元故障诊断提供依据。  相似文献   

Human Simulated Intelligence Motion Control for Three-link Acrobot on Horizontal Bar     

ZHANG Hu  LI Zu-shu  GU Jian-gong  CHEN Gui-qiang  TAN Zhi 《保鲜与加工》2007,(3):74-78

The design of multi-controller and multi-mode control law is presented for the three-link Acrobot on horizontal bar by employing Human simulated intelligent control method based on sensory-motor Schema.Imitated the characteristics of gymnast's swing-up and handstand movement,the control process of robot is divided by six sequence phase.Then the sensory cell and motor cell and the group of sensory-motor schema is designed based on the analysis of quality-quantity combination.The simulation results show that the controller ensures the gesture and the energy to the desired goal,farther more the motion control of giant swing backword is realized in the simulation.  相似文献   

BIM Based on Intelligent Parametric Modeling Technology   总被引：1，自引：0，他引：1  

ZENG Xu-dong  TAN Jie 《保鲜与加工》2006,(6):107-110

In order to push the informationization process of the building industry,promote sustainable architectural design and enhance the competitiveness of China's building industry,the authors study BIM(Building Information Modeling) based on intelligent parametric modeling technology.BIM is a new technology in the field of computer aided architectural design,which contains not only geometric data but also the great amount of engineering data throughout the lifecycle of a building.The authors also compare BIM technology with 2D CAD technology,and demonstrate the advantages and characteristics of intelligent parametric modeling technology.BIM,which is based on intelligent parametric modeling technology,will certainly replace traditional computer aided architectural design and become a new driving force to push forward China's building industry in this Information Age.  相似文献   

Enterprise Information Engineering Model and Its Integrated Support Environment     

TAN Wen-an 《保鲜与加工》2004,(12):73-77

By analyzing the process of enterprise information engineering construction and intra relationship among information system requirement, business process modeling, application system constructing and project management, one of 4 meta model composed of requirement engineering, process engineering, application system engineering and support engineering is proposed for enterprise information engineering. The developments and applications of the business processes and its application systems are integrated into the same development space and application space. Basing on this, a prototype system composed of requirement modeling environment, business process modeling environment, application system modeling environment, and support engineering environment has been designed and built, which is used for supporting enterprise information engineering constructing. It is valued for the enterprise information engineering construction in our country.  相似文献