Distribution and characterization of class 1 integrons in Salmonella enterica serotype Gallinarum biotype Gallinarum     

Kwon HJ  Kim TE  Cho SH  Seol JG  Kim BJ  Hyun JW  Park KY  Kim SJ  Yoo HS 《Veterinary microbiology》2002,89(4):303-309

Fowl typhoid caused by Salmonella enterica subsp. enterica serotype Gallinarum biotype Gallinarum is the most important chicken disease in Korea. Due to appearance of new or multiple antibiotics resistances in the recently isolated strains, it was difficult to control the disease using antibiotics in our country. Therefore, the prevalence and genetic contents of class 1 integrons in biotype Gallinarum isolated between 1992 and 2001 were investigated by PCR and direct sequencing, respectively. Out of 90 strains, 35 (39%) carried class 1 integrons. The 1.0, 1.6 and 2.0kbp amplicons were amplified in 32 strains (36%), 2 strains (2%) and 1 strain (1%), respectively. The 1.0, 1.6 and 2.0 kbp amplicons contained one (aadA1a), two (aadB-aadA1b) and three cassettes (dhfrXII-orfF-aadA2), respectively, providing resistances against aminoglycosides (aadA1a, aadA1b, aadB, and aadA2) and trimethoprim (dhfrXII). The integron-carrying strains of biotype Gallinarum appeared in 1996 and acquired additional cassettes in 2000. Although the resistances to ampicillin, tetracycline and chloramphenicol are unrelated to class 1 integrons, relatively high prevalence of integron in biotype Gallinarum may be a dormant threat to the chemotherapy of the disease in the near future because of potency to acquire additional antibiotics resistances.  相似文献   

A novel Henneguya species from channel catfish described by morphological, histological, and molecular characterization     

Griffin MJ  Pote LM  Wise DJ  Greenway TE  Mauel MJ  Camus AC 《Journal of aquatic animal health》2008,20(3):127-135

Channel catfish Ictalurus punctatus from a commercial farming operation in the Mississippi Delta were submitted for examination for the presence of infection by the trematode Bolbophorus damnificus. The fish were instead found to possess skin nodules suggestive of Henneguya pellis, a species previously described in the blue catfish I. furcatus. Despite the dermal location and distribution of lesions, morphological characteristics of the myxospores were inconsistent with H. pellis. Spores possessed a lanceolate spore body 15.4 +/- 1.5 microm (mean +/- SD; range = 12.2-19.3 microm) in length and 5.5 +/- 0.6 microm (range = 4.5-6.8 microm) in width in valvular view, and 4.7 +/- 0.2 microm (range = 4.2-5.0 microm) in width in sutural view. Polar capsules were pyriform and unequal in both length and width and contained polar filaments with six coils. Polar capsules measured 6.1 +/- 0.8 microm (range = 4.0-7.9 microm) long and 1.7 +/- 0.3 microm (range = 1.0-2.2 microm) wide. The caudal appendages were 50.5 +/- 8.3 microm (range = 34.8-71.4 micorm) long and the total length of the spore was 65.9 +/- 8.6 microm (range = 48.2-90.0 microm). The "blister like" plasmodia were round or ovoid, up to 2 mm in diameter, and randomly distributed throughout the epidermis of the fish. Histologically, plasmodia were confined to the dermis and elicited no inflammatory reaction from the fish. A blast search of the 18S small subunit rDNA sequence obtained by polymerase chain reaction amplification resulted in no identical sequence matches but indicated a close relationship to H. gurlei, H. ictaluri, and H. exilis. The unique host record, spore morphology, and novel genetic sequence derived from this isolate lead us to propose this isolate as a novel species, H. sutherlandi.  相似文献   

From wine to pepper: rotundone, an obscure sesquiterpene, is a potent spicy aroma compound   总被引：1，自引：0，他引：1  

Wood C  Siebert TE  Parker M  Capone DL  Elsey GM  Pollnitz AP  Eggers M  Meier M  Vössing T  Widder S  Krammer G  Sefton MA  Herderich MJ 《Journal of agricultural and food chemistry》2008,56(10):3738-3744

An obscure sesquiterpene, rotundone, has been identified as a hitherto unrecognized important aroma impact compound with a strong spicy, peppercorn aroma. Excellent correlations were observed between the concentration of rotundone and the mean 'black pepper' aroma intensity rated by sensory panels for both grape and wine samples, indicating that rotundone is a major contributor to peppery characters in Shiraz grapes and wine (and to a lesser extent in wine of other varieties). Approximately 80% of a sensory panel were very sensitive to the aroma of rotundone (aroma detection threshold levels of 16 ng/L in red wine and 8 ng/L in water). Above these concentrations, these panelists described the spiked samples as more 'peppery' and 'spicy'. However, approximately 20% of panelists could not detect this compound at the highest concentration tested (4000 ng/L), even in water. Thus, the sensory experiences of two consumers enjoying the same glass of Shiraz wine might be very different. Rotundone was found in much higher amounts in other common herbs and spices, especially black and white peppercorns, where it was present at approximately 10000 times the level found in very 'peppery' wine. Rotundone is the first compound found in black or white peppercorns that has a distinctive peppery aroma. Rotundone has an odor activity value in pepper on the order of 50000-250000 and is, on this criterion, by far the most powerful aroma compound yet found in that most important spice.  相似文献   

Circulating estradiol suppresses luteinizing hormone pulse frequency during dietary restriction     

Renquist BJ  Calvert CC  Adams BM  Adams TE 《Domestic animal endocrinology》2008,34(3):301-310

The influence of dietary restriction on the negative feedback potency of 17-beta-estradiol (E2) was evaluated in both castrated male (wethers) and female sheep (OVX ewes) during the breeding season. In study 1, OVX ewes received maintenance or restricted dietary energy for 7 weeks or maintenance energy for 6 weeks prior to a 5 day fast (n=12ewes/feeding group). Estradiol (0.31microg E2/50kg/h) or vehicle (10% EtOH-saline) was continuously infused into half the animals in each dietary treatment for the final 54h of the study. The dynamic pattern of LH secretion was assessed during the final 6h of infusion. Estradiol inhibited luteinizing hormone (LH) pulse amplitude independent of nutrition (P=0.02); fasting increased mean LH, LH peak height, and LH nadir in the absence of E2 (P=0.004, P=0.02, and P=0.02, respectively); while E2 inhibited pulse frequency (P=0.02) and increased peak width (P=0.04) in restricted ewes. Interestingly, despite uniform E2 delivery, serum concentrations of E2 differed with feeding status. Therefore, 12 wethers were infused with 0.31microg E2/50kg/h (6 fed, 6 fasted) and six wethers received 0.19microg E2/50kg/h (fasted) to establish similar serum concentrations of E2 in fed (0.31microg/50kg/h) and fasted (0.19microg/50kg/h) wethers. When fed and fasted wethers had uniform serum concentrations of E2 LH pulse frequency was suppressed (P<0.05) in fasted relative to fed animals, supporting the postulate that energy restriction enhances the E2 negative feedback potency. Collectively, these studies demonstrate that nutrition affects E2 feedback potency and clearance.  相似文献   

Effects of hyperbaric oxygen on full-thickness meshed sheet skin grafts applied to fresh and granulating wounds in horses     

Holder TE  Schumacher J  Donnell RL  Rohrbach BW  Adair HS 《American journal of veterinary research》2008,69(1):144-147

OBJECTIVE: To determine the effects of hyperbaric oxygen therapy (HBOT) on full-thickness skin grafts applied to fresh and granulating wounds of horses. ANIMALS: 6 horses. PROCEDURES: On day 0, two 4-cm-diameter circular sections of full-thickness skin were removed from each of 2 randomly selected limbs of each horse, and two 4-cm-diameter circular skin grafts were harvested from the pectoral region. A skin graft was applied to 1 randomly selected wound on each limb, leaving the 2 nongrafted wounds to heal by second intention. On day 7, 2 grafts were harvested from the pectoral region and applied to the granulating wounds, and wounds grafted on day 0 were biopsied. On day 14, 1 wound was created on each of the 2 unwounded limbs, and the wounds that were grafted on day 7 were biopsied. All 4 ungrafted wounds (ie, 2 fresh wounds and 2 wounds with 1-week-old granulation beds) were grafted. The horses then received HBOT for 1 hour daily at 23 PSI for 7 days. On day 21, the grafts applied on day 14 were biopsied. RESULTS: Histologic examination of biopsy specimens revealed that grafts treated with HBOT developed less granulation tissue, edema, and neovascularization, but more inflammation. The superficial portion of the graft was also less viable than the superficial portion of those not treated with HBOT. CONCLUSIONS AND CLINICAL RELEVANCE: The use of HBOT after full-thickness skin grafting of uncompromised fresh and granulating wounds of horses is not indicated.  相似文献   

Leading discussions on animal rights     

Blackwell TE  Rollin BE 《Journal of the American Veterinary Medical Association》2008,233(6):868-871

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Response to dietary phosphorus deficiency is affected by genetic background in growing pigs     

Alexander LS  Qu A  Cutler SA  Mahajan A  Lonergan SM  Rothschild MF  Weber TE  Kerr BJ  Stahl CH 《Journal of animal science》2008,86(10):2585-2595

Concern over the environmental effect of P excretion from pig production has led to reduced dietary P supplementation. To examine how genetics influence P utilization, 94 gilts sired by 2 genetic lines (PIC337 and PIC280) were housed individually and fed either a P-adequate diet (PA) or a 20% P-deficient diet (PD) for 14 wk. Initially and monthly, blood samples were collected and BW recorded after an overnight fast. Growth performance and plasma indicators of P status were determined monthly. At the end of the trial, carcass traits, meat quality, bone strength, and ash percentage were determined. Pigs fed the PD diet had decreased (P < 0.05) plasma P concentrations and poorer G:F (P < 0.05) over the length of the trial. After 4 wk on trial, pigs fed the PD diet had increased (P < 0.05) plasma 1,25(OH)(2)D(3) and decreased (P < 0.05) plasma parathyroid hormone compared with those fed the PA diet. At the end of the trial, pigs fed the PD diet had decreased (P < 0.05) BW, HCW, and percentage fat-free lean and tended to have decreased LM area (P = 0.06) and marbling (P = 0.09) and greater (P = 0.12) 10th-rib backfat than pigs fed the PA diet. Additionally, animals fed the PD diet had weaker bones and also decreased (P < 0.05) ash percentage and increased (P < 0.05) concentrations of 1alpha-hydroxylase and parathyroid hormone receptor mRNA in kidney tissue. Regardless of dietary treatment, PIC337-sired pigs consumed more feed and gained more BW than their PIC280-sired counterparts (P < 0.05) during the study. The PIC337-sired pigs also had greater (P < 0.05) HCW, larger (P < 0.01) LM area, and tended to have (P = 0.07) greater dressing percentage. Meat from the PIC337-sired pigs also tended to have greater (P = 0.12) concentrations of lactate but decreased (P = 0.07) concentrations of total glucose units 24 h postslaughter. Although plasma 1,25(OH)(2)D(3) concentrations were elevated (P < 0.05) in all the animals fed the PD diet, this elevation due to P deficiency tended (P = 0.09) to be greater in the PIC337-sired pigs after 12 wk on the treatment. The PIC337-sired pigs had stronger (P < 0.01) bones with greater ash percentage than the PIC280-sired pigs. The difference in the strength of the radii between the PIC337-sired pigs fed the PA and PD diets was greater than their PIC280-sired counterparts, which resulted in sire line x treatment interactions (P < 0.05). These data indicate differing mechanisms of P utilization between these genetic lines. Elucidating these mechanisms may lead to strategies to increase efficiency of growth in a more environmentally friendly manner.  相似文献   

Effects of ractopamine supplementation and postmortem aging on longissimus muscle palatability of beef steers differing in biological type     

Gruber SL  Tatum JD  Engle TE  Prusa KJ  Laudert SB  Schroeder AL  Platter WJ 《Journal of animal science》2008,86(1):205-210

Effects of ractopamine hydrochloride (RAC) supplementation and postmortem aging on palatability of beef from steers differing in biological type were evaluated using LM samples from British, Continental crossbred, and Brahman crossbred calf-fed steers (n = 98/type). Equal numbers of steers within each type were assigned to treatments of 0 or 200 mg.steer(-1).d(-1) of RAC fed during the final 28 d of the finishing period. Warner-Bratzler shear force (WBSF) was measured at 3, 7, 14, and 21 d postmortem, and trained sensory panel (TP) evaluation was conducted using LM samples aged for 14 d postmortem. A RAC x type interaction (P = 0.006) was detected for WBSF. Within each type, steers fed RAC produced steaks with greater (P < 0.05) WBSF values than steaks from control steers; however, the magnitude of the effect of RAC on WBSF was more pronounced among Brahman cross-breds (5.53 vs. 4.96 +/- 0.10 kg) than among Continental crossbred (4.16 vs. 3.96 +/- 0.10 kg) and British steers (4.10 vs. 3.75 +/- 0.10 kg). The effect of RAC on WBSF, though diminished slightly by aging (mean WBSF difference: 3 d = 0.49 kg; 21 d = 0.24 kg), was not completely mitigated by 21 d of postmortem storage (P(RAC x AGE) = 0.16). Steers fed RAC produced steaks that received lower (P < 0.05) TP ratings for tenderness (8.09 vs. 8.95 +/- 0.18) and juiciness (7.41 vs. 8.07 +/- 0.16 kg), along with slightly lower (P = 0.06) ratings for beef flavor (6.67 vs. 6.93 +/- 0.10 kg), compared with steaks from unsupplemented steers, regardless of biological type. Among the 3 biological types, Brahman crossbred cattle produced steaks with the greatest (P < 0.05) WBSF values at each aging period; WBSF values for steaks from British and Continental type steers did not differ (P > 0.05) at any aging time. Sensory panel ratings of tenderness, juiciness, and beef flavor were greatest (P < 0.05) for steaks from British steers, and least (P < 0.05) for steaks produced by Brahman-type steers. Results from this study suggest that RAC supplementation slightly decreases LM tenderness (WBSF and TP) of British, Continental crossbred, and Brahman cross-bred steers, and that the effect of RAC on WBSF may be more pronounced in steaks from Brahman crossbred cattle than among stenks from Continental type or British steers.  相似文献   

Pharmacokinetics and pharmacodynamics of meloxicam in piglets     

Fosse TK  Haga HA  Hormazabal V  Haugejorden G  Horsberg TE  Ranheim B 《Journal of veterinary pharmacology and therapeutics》2008,31(3):246-252

The pharmacokinetics and pharmacodynamics of meloxicam in piglets (16–23 days old) were studied using a stratified parallel group design. One group ( n  = 13) received 0.4 mg/kg meloxicam intravenously, while the other group ( n  = 12) received physiological saline solution. A carrageenan-sponge model of acute inflammation was used to evaluate the effects of meloxicam. The plasma clearance was low (0.061 L/kg/h), the volume of distribution was low (0.19 L/kg) and the elimination half-life was short (2.7 h). At most time points, the mean concentration of meloxicam in plasma exceeded the concentrations in exudate indicating a limited accumulation of the drug at the site of the inflammation. There were significant differences between the groups in the exudate prostaglandin E₂ (PGE₂) concentration, but the inhibition of PGE₂ in the meloxicam group was limited. The inhibition of thromboxane B₂ (TXB₂) production in serum in the meloxicam group was extensive, but of shorter duration than the PGE₂ inhibition in exudate.  相似文献   

Temporal-spatial epidemiology of foot-and-mouth disease outbreaks in Mongolia, 2000 – 2002     

Shiilegdamba E  Carpenter TE  Perez AM  Thurmond MC 《Veterinary research communications》2008,32(3):201-207

Prior to 2000, foot-and-mouth disease (FMD) had not been observed in Mongolia since 1973; however, between April 2000 and July 2002, Mongolia reported 44 FMD outbreaks that affected cattle, sheep, goats, and camels. The objectives of this study were to describe the distributions of the 44 reported FMD outbreaks in Mongolia and to assess their spatial clustering and directions of movement. Official reports were collected to obtain the number and species of animals both affected and at risk, and the date and geographical coordinates of each outbreak. Significant global and local spatial clusters of reported FMD outbreaks were identified. Disease spread during the second epidemic moved 76° northeast and the spread of the disease during the third epidemic moved 110° northwest. FMD outbreaks were clustered intensely close to other FMD-positive counties. These findings can be used in the future to help plan prevention and control measures in high risk areas.  相似文献