Localization of Oestrogen Receptors in the Epididymis During Sexual Maturation of the Domestic Cat     

J Schön  S Neumann  DE Wildt  BS Pukazhenthi  K Jewgenow 《Reproduction in domestic animals》2009,44(S2):294-301

Oestrogens are involved in regulation of spermatogenesis and sperm maturation and are essential for male fertility. To study the role of oestrogens on epididymal function in the domestic cat, we analyzed the localization patterns of oestrogen receptors (ERs) within the epididymis of juvenile, pubertal and adults using immunohistochemistry. Cat epididymal tissues obtained during routine castrations were fixed in chilled Bouin's solution and processed for immunohistochemistry with ER-specific antibodies. For a certain receptor type, ER localization was influenced by donor age. In the juvenile epididymis, ERα was localized in the nuclei of epithelial cells of efferent ducts and undifferentiated epithelium of the ductus epididymis. During puberty, ERα localization in the undifferentiated epithelium of the epididymis shifted from the nuclei to the cytoplasm and plasma membrane. Oestrogen receptor-α level was highest in the pubertal and adult epididymis, especially within the cytoplasm and in plasma membranes of caput epithelial cells. This finding was suggestive of a role in fluid reabsorption within the efferent ducts and the epididymis. In corpus and cauda regions, ERα was less abundant, suggesting a minor role for oestrogens in sperm storage areas. Interestingly, localization of ERβ was neither influenced by age nor location within the epididymis and was ubiquitous throughout. Results demonstrate that oestrogen actions within the epididymis may be predominantly mediated through ERα during sexual maturation in the domestic cat.  相似文献   

Follicular Morphology, Oocyte Diameter and Localisation of Fibroblast Growth Factors in the Domestic Dog Ovary     

N Songsasen  A Fickes  BS Pukazhenthi  DE Wildt 《Reproduction in domestic animals》2009,44(S2):65-70

  相似文献   

CAN SONOGRAPHIC FINDINGS PREDICT THE RESULTS OF LIVER ASPIRATES IN DOGS WITH SUSPECTED LIVER DISEASE?     

MARTIN GUILLOT  MARC-ANDRÉ D'ANJOU  KATE ALEXANDER  CHRISTIAN BÉDARD  MICHEL DESNOYERS  GUY BEAUREGARD  JÉRÔME R. E. DEL CASTILLO 《Veterinary radiology & ultrasound》2009,50(5):513-518

While abdominal ultrasound and ultrasound-guided fine-needle aspiration cytology are often combined to help determine the type of liver disease in dogs, little is known about the relationship that may exist between the results of these tests. We hypothesized that specific sonographic findings, or combinations of findings, may predict results of liver ultrasound-guided fine-needle aspiration cytology. Hepatic and extrahepatic sonographic findings were recorded prospectively using a standardized form in 70 dogs with clinically suspected liver disease and in which liver ultrasound-guided fine-needle aspiration cytology was performed. The predictive value of sonographic findings in regard to the category of cytology results was assessed with stepwise logistic regression analysis. Sonographic detection of a hepatic mass (≥3 cm; risk ratio [RR] 3.83, 95% Wald confidence intervals [95% CI] 2.42–3.93, P =0.0036), ascites (RR 3.82, 95% CI 1.94–4.28, P =0.0044), abnormal hepatic lymph node(s) (RR 3.01, 95% CI 1.22–4.88, P =0.0262), and abnormal spleen (RR 3.26, 95% CI 1.20–3.85, P =0.0274) were the most predictive of liver neoplasia on cytology. Conversely, sonographic detection of hepatic nodules (<3 cm; RR 1.97, 95% CI 0.95–2.96, P =0.0666) was most predictive of vacuolar hepatopathy on cytology. In dogs with suspected liver disease, several sonographic findings, alone or combined, are thus predictive of liver ultrasound-guided fine-needle aspiration cytology results. In the light of the fact that ultrasound-guided fine-needle aspiration cytology of the liver has limitations, these predictabilities could influence the selection of diagnostic tests to reach a reliable diagnosis.  相似文献   

Pelvic limb kinematics in the dog with and without a stifle orthosis          下载免费PDF全文

Bryan T. Torres DVM  PhD  DACVS‐SA  Yang‐Chieh Fu PhD  Gabriella S. Sandberg BS  Steven C. Budsberg DVM  MS  DACVS 《Veterinary surgery : VS》2017,46(5):642-652

  相似文献   

Laparoscopic evaluation of the small intestine in the standing horse: Technique and effects*          下载免费PDF全文

Andrew R. E. Jones BVetMed MRCVS  Claude A. Ragle DVM  DACVS  DABVP  Dusty Anderson BS  Coryelle Scott BS 《Veterinary surgery : VS》2017,46(6):812-820

  相似文献   

Outcome and complications in dogs with appendicular primary bone tumors treated with stereotactic radiotherapy and concurrent surgical stabilization          下载免费PDF全文

Sarah E. Boston DVM  DVSc  Diplomate ACVS  ACVS Founding Fellow of Surgical Oncology  Arathi Vinayak DVM  Diplomate ACVS  Xiaomin Lu BS  MA  MS  PhD  Jason A. Bleedorn DVM  Diplomate ACVS 《Veterinary surgery : VS》2017,46(6):829-837

  相似文献   

Preparation of Canine and Feline Cadavers for Surgical Laboratories     

KYLE G. MATHEWS DVM  MS  Diplomate ACVS  KRISTEN RILEY BS  CVT  WILLIAM S. DERNELL DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2010,39(2):224-225

  相似文献   

In Vitro Biomechanical Evaluation and Comparison of FiberWire,FiberTape, OrthoFiber,and Nylon Leader Line for Potential Use During Extraarticular Stabilization Of Canine Cruciate Deficient Stifles     

RICHARD BURGESS BVM&S  STEVE ELDER BS  MS  PhD  RON McLAUGHLIN DVM  DVSc  Diplomate ACVS  PETER CONSTABLE BVSc  MS  PhD  Diplomate ACVIM 《Veterinary surgery : VS》2010,39(2):208-215

Objective— To compare biomechanical properties of 3 new generation polyethylene sutures (FiberTape [FT], FiberWire [FW], and OrthoFiber [OF]) with nylon leader line (NL) for use during extraarticular fixation of cranial cruciate deficient stifles. Study Design— In vitro biomechanical testing of suture loops under monotonic tensile and cyclical loading until failure. Sample Population— Constructs of FT, FW, OF, and NL. Methods— Twenty loops of each of 12 combinations of fixation and suture had monotonic tensile and cyclical loading. Two knotting techniques (square knot [SQ], slip knot [SL]) and a crimp clamp (CR) system were evaluated. Elongation, stiffness, and strength of constructs was tested. The main effects of group, loop material, and their interaction were evaluated. Results— Knotted FT, FW, and OF had less elongation than knotted NL under monotonic tensile and cyclical loading. Under monotonic tensile loading, knotted FT and OF were stiffer than knotted NL. CR FT, CR FW, and CR OF were stiffer than CR NL and CR FT, CR FW, and CR OF were stiffer than knotted FT, FW, and OF. FW and OF knotted loops were weaker than knotted NL. CR FT was stronger than CR NL. CR FT and CR OF were weaker than knotted FT and OF. Conclusions— Polyethylene sutures are stronger, stiffer and elongate less than nylon leader. Crimping suture alters the biomechanical properties of the loop. Clinical Relevance— FW, FT, and OF may perform better in reconstructive procedures, where increased strength and stiffness are considered to be beneficial.  相似文献   

CONCOMITANT LIPOSOMAL DOXORUBICIN AND DAILY PALLIATIVE RADIOTHERAPY IN ADVANCED FELINE SOFT TISSUE SARCOMAS     

MIRIAM KLEITER  ALEXANDER TICHY  MICHAEL WILLMANN  MAXIMILIAN PAGITZ  BIRGITT WOLFESBERGER 《Veterinary radiology & ultrasound》2010,51(3):349-355

Local recurrence of feline soft tissue sarcomas is common despite aggressive treatment. Liposomal doxorubicin might serve as a depot radiosensitizer if administered concomitantly with daily radiotherapy and thus improve tumor control. In this pilot study, the feasibility of concomitant liposomal radiochemotherapy was evaluated in a palliative setting in 10 cats with advanced soft tissue sarcomas. Cats were treated with median number of 5 (range 5–7) daily fractions of radiotherapy and a median total dose of 20 Gy (range 20–31.5 Gy). One dose of liposomal doxorubicin was administered at the beginning of radiotherapy. Seven cats received further free or liposomal doxorubicin after completion of the liposomal doxorubicin/radiation protocol. Seven of the treated 10 cats (70%) achieved a partial (n=5) or complete (n=2) response with a median response duration of 237 days. The median progression free interval in all 10 cats was 117 days and the median overall survival time was 324 days. Concomitant liposomal radiochemotherapy was tolerated well in nine cats, one cat experienced temporary anorexia. Although the number of patients is too small to make definitive conclusions, results appear promising enough to investigate the role of liposomal doxorubicin as a radiosensitizer further.  相似文献   

Flavonoids in Food and Their Health Benefits   总被引：17，自引：6，他引：17  

Yao LH  Jiang YM  Shi J  Tomás-Barberán FA  Datta N  Singanusong R  Chen SS 《Plant foods for human nutrition (Dordrecht, Netherlands)》2004,59(3):113-122

There has been increasing interest in the research of flavonoids from dietary sources, due to growing evidence of the versatile health benefits of flavonoids through epidemiological studies. As occurrence of flavonoids is directly associated with human daily dietary intake of antioxidants, it is important to evaluate flavonoid sources in food. Fruits and vegetables are the main dietary sources of flavonoids for humans, along with tea and wine. However, there is still difficulty in accurately measuring the daily intake of flavonoids because of the complexity of existence of flavonoids from various food sources, the diversity of dietary culture, and the occurrence of a large amount of flavonoids itself in nature. Nevertheless, research on the health aspects of flavonoids for humans is expanding rapidly. Many flavonoids are shown to have antioxidative activity, free-radical scavenging capacity, coronary heart disease prevention, and anticancer activity, while some flavonoids exhibit potential for anti–human immunodeficiency virus functions. As such research progresses, further achievements will undoubtedly lead to a new era of flavonoids in either foods or pharmaceutical supplements. Accordingly, an appropriate model for a precise assessment of intake of flavonoids needs to be developed.Most recent research has focused on the health aspects of flavonoids from food sources for humans. This paper reviews the current advances in flavonoids in food, with emphasis on health aspects on the basis of the published literature, which may provide some guidance for researchers in further investigations and for industries in developing practical health agents.  相似文献