Proteomic analysis of membrane preparations from developing Pinus radiata compression wood     

Mast S  Peng L  Jordan TW  Flint H  Phillips L  Donaldson L  Strabala TJ  Wagner A 《Tree physiology》2010,30(11):1456-1468

For coniferous gymnosperms, few data exist as to the contribution of the membrane-associated proteome to cell wall and wood formation. In this study, we begin to address this knowledge deficiency by examining the proteomic profile of Golgi-enriched membrane preparations derived from developing Pinus radiata compression wood. These membrane preparations were generated by a combination of discontinuous sucrose gradient centrifugation and Triton X-114-based phase separation. Fractionation by phase separation removed contaminating proteins associated with the cytoskeleton and enabled the discrimination between soluble and membrane-bound/integral proteins. The proteomic analysis of the resulting aqueous and detergent phases using high-performance liquid chromatography-tandem mass spectrometry resulted in the identification of 175 proteins. The majority of the identified proteins were membrane bound/integral and originated from cellular components such as the nucleus, plastids, endoplasmic reticulum, plasma membrane and Golgi vesicles. On the basis of bioinformatic analysis, many of the identified proteins were predicted to be involved either in the regulation of wood formation or in cell wall biosynthesis, which indicated that the proteomic analysis of non-cytosolic proteins in developing xylem is a useful strategy to investigate the molecular aspects of wood formation in pine.  相似文献   

Airborne radiometric survey data and a DTM as covariates for regional scale mapping of soil organic carbon across Northern Ireland     

B. G. Rawlins    B. P. Marchant    D. Smyth    C. Scheib    R. M. Lark  & C. Jordan 《European Journal of Soil Science》2009,60(1):44-54

Soil scientists require cost-effective methods to make accurate regional predictions of soil organic carbon (SOC) content. We assess the suitability of airborne radiometric data and digital elevation data as covariates to improve the precision of predictions of SOC from an intensive survey in Northern Ireland. Radiometric data (K band) and, to a lesser extent, altitude are shown to increase the precision of SOC predictions when they are included in linear mixed models of SOC variation. However the statistical distribution of SOC in Northern Ireland is bimodal and therefore unsuitable for geostatistical analysis unless the two peaks can be accounted for by the fixed effects in the linear mixed models. The upper peak in the distribution is due to areas of peat soils. This problem may be partly countered if soil maps are used to classify areas of Northern Ireland according to their expected SOC content and then different models are fitted to each of these classes. Here we divide the soil in Northern Ireland into three classes, namely mineral, organo-mineral and peat. This leads to a further increase in the precision of SOC predictions and the median square error is 2.2 %². However a substantial number of our observations appear to be mis-classified and therefore the mean squared error in the predictions is larger (30.6 %²) since it is dominated by large errors due to mis-classification. Further improvement in SOC prediction may therefore be possible if better delineation between areas of large SOC (peat) and small SOC (non-peat) could be achieved.  相似文献   

Prevalence and antimicrobial resistance of MRSA across different pig age groups in an intensive pig production system in Australia     

Shafi Sahibzada  Stanley Pang  Marta Hernndez‐Jover  David Jordan  Sam Abraham  Mark O'Dea  Jane Heller 《Zoonoses and public health》2020,67(5):576-586

This observational study aimed to determine MRSA prevalence using strain‐specific real‐time PCR at the pig level, stratified by age groupings, within a pig enterprise. A total of 658 samples were collected from individual pigs (n = 618) and the piggery environment (n = 40), distributed amongst five different pig age groups. Presumptive MRSA isolates were confirmed by the presence of mecA, and MALDI‐TOF was performed for species verification. All isolates were tested against 18 different antimicrobials. MRSA was isolated from 75.2% (95% CI 71.8–78.6) of samples collected from pigs, and 71% of the MRSA isolates from this source were identified as community‐associated (CA)‐MRSA ST93, while the remainder were livestock‐associated (LA)‐MRSA ST398. Amongst environmental isolates, 80% (CI 64.3–95.7) were ST93 and the remainder ST398. All MRSA isolates from pigs and the environment were susceptible to ciprofloxacin, gentamicin, linezolid, mupirocin, rifampicin, sulfamethoxazole–trimethoprim, teicoplanin and vancomycin. Phenotypic rates of resistance were penicillin (100%), clindamycin (97.6%), erythromycin (96.3%), ceftiofur (93.7%), chloramphenicol (81.2%), tetracycline (63.1%) and amoxicillin–clavulanate (63.9%). A low prevalence of resistance (9.2%) was observed against neomycin and quinupristin–dalfopristin. The probability of MRSA carriage in dry sows (42.2%) was found to be significantly lower (p < .001) when compared to other age groups: farrowing sows (76.8%, RR1.82), weaners (97.8%, RR 2.32), growers (94.2%, RR 2.23) and finishers (98.3%, RR 2.33). Amongst different production age groups, a significant difference was also found in antimicrobial resistance for amoxicillin–clavulanate, neomycin, chloramphenicol and tetracycline. Using the RT‐PCR assay adopted in this study, filtering of highly prevalent ST93 and non‐ST93 isolates was performed at high throughput and low cost. In conclusion, this study found that weaner pigs presented a higher risk for CA‐MRSA and antimicrobial resistance compared to other age groups. These findings have major implications for how investigations of MRSA outbreaks should be approached under the One‐Health context.  相似文献   

An Improved Synthesis of 7, 8-Epoxy-1,3,11-cembratriene- 15R(α), 16-diol,a Cembranoid of Marine Origin with a Potent Cancer Chemopreventive Activity     

Hesham Fahmy  Sherief I. Khalifa  Takao Konoshima  Jordan K. Zjawiony 《Marine drugs》2004,2(1):1-7

An effective method for the synthesis of 7,8-epoxy-1,3,11-cembratriene- 15R(α),16-diol and its in vitro Epstein-Barr Virus Early Antigen (EBV-EA) Activation Chemopreventive Assay are reported. This semisynthetic product is a new cembranoid with a potent tumor inhibitory activity that is expected to be a lead compound for a new class of chemopreventive agents of marine origin.  相似文献   

Detection of antibodies to equine arteritis virus by a monoclonal antibody-based blocking ELISA.   总被引：2，自引：0，他引：2       下载免费PDF全文

H J Cho  S C Entz  D Deregt  L T Jordan  P J Timoney    W H McCollum 《Canadian journal of veterinary research》2000,64(1):38-43

A potent ELISA antigen was prepared from equine arteritis virus (EAV) by differential centrifugation of EAV-infected cell culture fluid, followed by solubilization of the preparation by Triton X-100 treatment. Using this antigen and a mouse monoclonal antibody against the G(L) protein of EAV, a reliable blocking ELISA (bELISA) was developed for the detection of EAV antibodies in equine sera. The bELISA was evaluated using a total of 837 test serum samples. The relative sensitivity (n = 320) of the bELISA compared to the serum neutralization (SN) test was 99.4%. The bELISA appears to be a highly specific test, the specificity of which did not appear to be adversely affected by previous exposure of horses to non-EAV-containing biologicals. Of 119 serum samples, 21 from horses without any history of exposure to EAV and 98 from racetrack Thoroughbreds, 118 were negative in the SN test and bELISA. One sample was SN-negative but suspicious with the bELISA. Based on testing 465 SN-negative field samples and 52 SN-negative samples from experimental horses, and excluding any sera giving a suspicious reaction, the relative specificity of the bELISA was 97.7%. Samples should be examined undiluted and diluted 1/10 in the bELISA because the testing of sera of high neutralizing antibody titer may be affected by a prozone-like phenomenon. The bELISA is a more rapid and cost-efficient test than the SN test for the detection of EAV antibodies in equine sera.  相似文献   

Empirical Models for Predicting the Dry Matter Yield of Grass Silage Swards Using Plant Tissue Analyses     

Bailey  J. S.  Higgins  A.  Jordan  C. 《Precision Agriculture》2000,2(2):131-145

Quantifying spatial variability in forage grass yield within individual fields is hampered by the lack of accurate yield monitoring equipment. Here, it is shown how dry matter (DM) yield of silage swards can be predicted on the basis of their mineral composition. This empirical method of predicting yield enables diagnoses of sward nutrient status to be made simultaneously from the tissue test information, and provides a unique opportunity for identifying the nutritional and non-nutritional factors responsible for variability in sward productivity at sub-field scales. Maps of sward DM yield at first, second and third cut silage stages in 1999, and at first cut silage stage in 2000, on a large (7.9 ha) grassland field were produced using two different yield models: one model for first cut and a separate model for second and third cuts. The maps indicated that DM production varied considerably across the field, particularly at first cut, but that the pattern of yield variability at this cut was consistent from 1999 to 2000. The results of the plant tissue tests suggested that N deficiency had been responsible for limiting DM production on the lower yielding parts of the field.  相似文献   

Strategic use of anthelmintics in working donkeys in Mediterranean climatic conditions     

D H Bliss  E D Svendsen  I E Georgoulakis  S Grosomanidis  F Taylor  W J Jordan 《The Veterinary record》1985,117(23):613-614

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Gordon memorial lecture: people, poultry and pathogenic mycoplasmas     

F T Jordan 《British poultry science》1985,26(1):1-15

Man in managing poultry for human benefit has found that his methods may precipitate or exacerbate certain diseases: among which are the mycoplasmosis of fowls and turkeys. Control measures for these conditions have therefore been sought and to make them effective it has been necessary to study the epidemiology, diagnosis and pathogenesis of these conditions. Much is now known about their epidemiology although certain aspects still require investigation. More sensitive and specific methods of diagnosis are also required and although it is possible to control these conditions by eradication the mycoplasmosis are still an economic problem. Under existing management systems, therefore, development and use of effective vaccines and additional drugs are essential. It is noteworthy that the pathogenesis of at least one of the avian mycoplasmosis is similar to human immune-complex disease. In further elucidation of this complex the fowl may be a useful experimental animal. Thus the association of people, poultry and pathogenic mycoplasmas not only involves attempts to control the pathogens in order to attain maximum production by poultry but also could include the utilisation of poultry and the pathogen in an attempt to elucidate some of the diseases of people.  相似文献   

Myelitis in a cat infected with Toxoplasma gondii and feline immunodeficiency virus   总被引：1，自引：0，他引：1  

J R Heidel  J P Dubey  L L Blythe  L L Walker  J R Duimstra  J S Jordan 《Journal of the American Veterinary Medical Association》1990,196(2):316-318

Severe necrotizing myelitis secondary to localization and reactivation of Toxoplasma gondii within the spinal cord of a domestic shorthair cat was diagnosed by use of light and electron microscopy and immunohistochemistry. The cat also was infected with feline immunodeficiency virus. This case may have useful comparative features to T gondii infections in human patients with acquired immunodeficiency syndrome.  相似文献   

SAMUEL GARMAN--1843-1927     

Jordan DS  Barbour T 《Science (New York, N.Y.)》1928,67(1731):232-233

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