DOES MEASUREMENT OF SMALL INTESTINAL DIAMETER INCREASE DIAGNOSTIC ACCURACY OF RADIOGRAPHY IN DOGS WITH SUSPECTED INTESTINAL OBSTRUCTION?     

Taízha C. Ciasca  Frederic H. David  Christopher R. Lamb 《Veterinary radiology & ultrasound》2013,54(3):207-211

The ratio between maximal small intestinal (SI) diameter and the height of the body of the fifth lumbar vertebra (L5) in radiographs has been reported as a diagnostic test in dogs with suspected intestinal obstruction. In order to assess the effect of the SI/L5 ratio on the accuracy of radiographic diagnosis of intestinal obstruction, lateral abdominal radiographs of 37 dogs with small intestinal obstruction and 48 nonobstructed dogs were mixed and examined independently by six observers who were unaware of the final diagnosis and who represented a range of experience. Observers first examined radiographs subjectively and stated the likelihood of obstruction (definitely not, probably not, equivocal, probably, definitely). Observers subsequently reexamined the radiographs, determined the SI/L5 ratio, and again stated the likelihood of obstruction. The most frequent cause of obstruction was foreign body (29/37, 78%). Dogs with SI obstruction had a significantly larger median SI/L5 ratio than nonobstructed dogs (P = 0.0002). Using an SI/L5 ratio of 1.7 for diagnosis of intestinal obstruction, sensitivity and specificity were 66%. Use of the SI/L5 ratio was not associated with increased accuracy of diagnosis for any observer, regardless of experience, hence this test may have no diagnostic impact.  相似文献   

Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.)     

Dušan Jankov  Dušanka Inđić  Petar Kljajić  Radmila Almaši  Goran Andrić  Slavica Vuković  Mila Grahovac 《Journal of pest science》2013,86(2):211-216

The aim of the study was to investigate how various types of storage facilities with, e.g., concrete, metal, and plywood surfaces interfere with the activity of different insecticide formulations used for rice weevil Sitophilus oryzae (L.) control: malathion (EC), pirimiphos-methyl (EC), and lambda-cyhalothrin (CS and WP). Initial and residual efficacy were determined in the laboratory. Knockdown data for the initial effects were processed by probit analysis and presented as knockdown time (KDT) parameters with kdt-p lines. Delayed effects were shown as knockdown efficacy (%) determined after 24 h of weevils’ contact with 7-, 14-, 30-, 60-, 90-, 120-, 150-, and 180-day-old deposits on each surface. Malathion (EC) and pirimiphos-methyl (EC) showed the highest initial knockdown efficacy on metal, while it was 3.6 (3.4)- and 4.4 (3.3)-fold lower on concrete and plywood, respectively. Lambda-cyhalothrin (CS and WP) showed the highest initial efficacy on concrete, and slightly lower (1.3 and 2.4) fold on metal and plywood, respectively. Both formulations of lambda-cyhalothrin and malathion on metal, as well as pirimiphos-methyl on plywood were 100 % efficient against S. oryzae 180 days after the treatment. Delayed efficacy of both formulations of lambda-cyhalothrin decreased on plywood after 120 days, and after 180 days the efficacy was 55 %. All insecticides, except lambda-cyhalothrin (CS), expressed low knockdown efficacy on concrete, while the deposit of lambda-cyhalothrin (CS) on concrete was 100 % efficient during 90 days, and after 120, 150, and 180 days the efficacy was 83, 65, and 17 %, respectively.  相似文献   

Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine     

Marcos Mancilla  María-Jesús Grilló  María-Jesús de Miguel  Ignacio López-Go?i  Beatriz San-Román  Ana Zabalza-Baranguá  Ignacio Moriyón 《Veterinary research》2013,44(1):105

Brucella melitensis Rev 1 is the best vaccine available for the prophylaxis of small ruminant brucellosis and, indirectly, for reducing human brucellosis. However, Rev 1 shows anomalously high rates of spontaneous dissociation from smooth (S) to rough (R) bacteria, the latter being inefficacious as vaccines. This S-R instability results from the loss of the O-polysaccharide. To overcome this problem, we investigated whether some recently described mechanisms promoting mutations in O-polysaccharide genes were involved in Rev 1 S-R dissociation. We found that a proportion of Rev 1 R mutants result from genome rearrangements affecting the wbo O-polysaccharide loci of genomic island GI-2 and the wbkA O-polysaccharide glycosyltransferase gene of the wbk region. Accordingly, we mutated the GI-2 int gene and the wbk IS transposase involved in those arrangements, and found that these Rev 1 mutants maintained the S phenotype and showed lower dissociation levels. Combining these two mutations resulted in a strain (Rev 2) displaying a 95% decrease in dissociation with respect to parental Rev 1 under conditions promoting dissociation. Rev 2 did not differ from Rev 1 in the characteristics used in Rev 1 typing (growth rate, colonial size, reactivity with O-polysaccharide antibodies, phage, dye and antibiotic susceptibility). Moreover, Rev 2 and Rev 1 showed similar attenuation and afforded similar protection in the mouse model of brucellosis vaccines. We conclude that mutations targeting genes and DNA sequences involved in spontaneous O-polysaccharide loss enhance the stability of a critical vaccine phenotype and complement the empirical stabilization precautions taken during S Brucella vaccine production.  相似文献   

Genotyping of Bacillus anthracis Isolated from Croatia and Bosnia and Herzegovina     

I. Steiner  I. Račić  S. Špičić  B. Habrun 《Zoonoses and public health》2013,60(3):202-208

Anthrax is a serious disease caused by Bacillus anthracis. Humans can become infected by handling products from infected animals, by breathing spores and rarely by eating undercooked meat from infected animals. The genome of B. anthracis is highly monomorphic and thus shows very low DNA sequence variation. We analysed the molecular characteristics of 12 B. anthracis isolates from outbreaks in Croatia and Bosnia and Herzegovina, which have occurred during the past 10 years along with two vaccine strains. Genotyping system based on variable‐number tandem repeat analysis at six loci revealed that six isolates belong to genotype from the A1.a cluster whilst six isolates relate to the B2 cluster, compared to 89 previously described genotypes. The distribution of two evolutionarily distant clusters suggests an introduction of B. anthracis to this area in at least two separate events.  相似文献   

Distribution and Genotypic Characterization of Campylobacter jejuni Isolated from Poultry in Split and Dalmatia County,Croatia     

A. Kovačić  I. Listeš  C. Vučica  L. Kozačinski  I. Tripković  K. Šiško‐Kraljević 《Zoonoses and public health》2013,60(4):269-276

Consumption of poultry contaminated with Campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. Therefore, the aim of our study was to investigate the prevalence and genotype diversity of Campylobacter jejuni in poultry meat intended for consumption in Split and Dalmatia County, which is the second biggest County in Croatia. Furthermore, we also wanted to discover possibly stable clones of C. jejuni appearing in different samples and periods of time, which would indicate their ability to persist in or adapt to poultry. In the period from March 2008 until June 2010, 834 samples of poultry from various sources were examined using a surface swab technique. Isolation of C. jejuni was performed by Preston broth and Karmali agar. Identification of the isolates was carried out using biochemical tests. C. jejuni was found in 84 of 574 chicken samples (14.6%) and in nine of 260 samples of turkey (3.5%). Pulse‐field gel electrophoresis (PFGE) was used to analyse 61 obtained isolates using SmaI and KpnI. Of 22 different macrorestriction profiles (MRP) that were found, five were detected in poultry from both different locations and periods of time. Samples from 11 locations were found to be contaminated with more than two different genotypes of C. jejuni. Interestingly, the same MRP were found both in poultry declared to be of domestic origin and in the poultry imported from abroad. The prevalence of C. jejuni in poultry samples was in accordance with previously reported results. Genotypic analysis indicated that the population of C. jejuni in Split and Dalmatia County was diverse and that multiple strains of C. jejuni could be found in the same poultry samples. Furthermore, the same genotypes were identified from the samples obtained from different locations and periods of time, which could support the theory of a global existence of certain MRP that are able to persist in or adapt to poultry.  相似文献   

Fruit Physical Features,Phenolic Compounds Profile and Inhibition Activities of Cranberry Cultivars (Vaccinium macrocarpon) Compared to Wild-Grown Cranberry (Vaccinium oxycoccus)     

Narwojsz  Agnieszka  Tańska  Małgorzata  Mazur  Barbara  Borowska  Eulalia Julitta 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(3):300-306

Plant Foods for Human Nutrition - This study analysed fruits of cranberry cultivars: Ben Lear, Bergman, Early Richard, Pilgrim and Stevens and compared them with wild-grown cranberry fruits. The...  相似文献   

Sedimentological and geochemical characterization of river suspended particulate matter (SPM) sampled by time-integrated mass flux sampler (TIMS) in the Sava River (Croatia)     

Lučić  Mavro  Jurina  Irena  Ščančar  Janez  Mikac  Nevenka  Vdović  Neda 《Journal of Soils and Sediments》2019,19(2):989-1004

Purpose

Suspended particulate matter (SPM) plays a crucial role in the transport of natural and anthropogenic substances. However, obtaining a representative sample and a substantial amount of SPM in rivers, where the flux and quantity of SPM are highly episodic, can be a challenging task. To collect a sufficient quantity of SPM for detailed sedimentological and geochemical analysis, a time-integrated mass flux sampler (TIMS) was used for the sampling in a medium size river (the Sava River, Croatia).

Materials and methods

Sampling was conducted in August and October 2014, and February and May 2015 under different discharge conditions. The SPM collected by TIMS was characterized with respect to its particle size distribution (PSD), mineral content, and geochemical composition.

Results and discussion

PSD analysis identified silt as a dominant size fraction in all samples except in May 2015 when sand prevailed; subsequent chemical dispersion of samples revealed flocculation as the main factor responsible for the resulting PSD. The mineralogical composition of the SPM was quite constant (quartz, calcite, dolomite, feldspar, illite/muscovite, kaolinite), but the contribution of particular mineral varied depending on the sampling period. In May 2015, unusually high calcite content was determined. Though the dominant source is still uncertain, a portion of calcite is likely detrital in origin. Geochemical analyses of collected material revealed significant anthropogenic input of ecotoxic elements (Ni, As, Cr, Pb, Bi, Cd, Zn, Sb) primarily associated with the fine fraction of the Sava River SPM.

Conclusions

A considerable amount of the SPM was collected by TIMS, both during high and low river discharge. Sedimentological analyses of the Sava River SPM suggested the input of material from various sources during different river regimes. Geochemical composition of the SPM followed its sedimentological characteristics—preferential adsorption of trace elements to fine-grained clay mineral particles was documented. Some processes inside TIMS were observed during this investigation—the suspended material captured inside the sampler underwent additional changes. The flocculation of the colloidal material instigated by algal bloom was observed.

  相似文献   

Transformation of Petroleum Saturated Hydrocarbons during Soil Bioremediation Experiments     

Branimir Jovančićević  Mališa Antić  Ivona Pavlović  Miroslav Vrvić  Vladimir Beškoski  Alexander Kronimus  Jan Schwarzbauer 《Water, air, and soil pollution》2008,190(1-4):299-307

This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pan?evo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P₁–P₁₂ samples). Organic substance was extracted by Soxhlet’s method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P₁. Pristane and phytane occurred in sample P₈, and in even higher quantities in the final sample P_12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P₁ the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P₈ and P₁₂ because of the reduced concentration. The observed changes in the alkane fractions’ compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.  相似文献   

Fruit set evaluation for self-compatibility selection in almond     

O. Kodad  R. Socias i Company   《Scientia Horticulturae》2008,118(3):260-265

Self-compatibility was assessed in 19 almond selections by genetic and physiological means, such as PCR with specific primers for the S_f allele, pollen tube growth, and fruit set after self-pollination and in bagged branches. Although all genotypes possessed the S_f allele and showed a similar pollen tube growth after self-pollination than after cross-pollination with cross-compatible pollen, fruit sets showed a different behaviour between years, ranging from 16.2 to 24.7%, as well as between treatments, with the highest after self-pollination. The differences between genotypes could be due to the genetic constitution of each genotype, where inbreeding may reduce set by the accumulation of deleterious genes in different members of a progeny. Flower morphology may also affect sets in bagged branches. Thus, in addition to bud density, flower sterility, pollination success and environmental conditions, other traits must be taken into account when evaluating yield in self-compatible almond cultivars, such as the inbreeding effect and the effective autonomous self-pollination.  相似文献   

Comparison of Climate Change Effects on Pome And Stone Fruit Phenology Between Balkan Countries and Bonn/Germany     

Pakeza Drkenda  Osman Musić  Slađana Marić  Darko Jevremović  Sanja Radičević  Metka Hudina  Sabina Hodžić  Achim Kunz  Michael M. Blanke 《Erwerbs-Obstbau》2018,60(4):295-304

The objective of the joint project between the two universities of Bonn and Sarajevo was to study the effects of climate change associated with warmer winter and spring temperatures on fruit tree phenology. Changes in flowering date of apple and cherry were correlated with local weather data obtained concomitantly on-site at four locations in the Balkan countries Slovenia, Serbia, B & H and at Bonn, Germany.Phenological data of flower opening (F1; BBCH 61) of apple cv. ‘Golden Delicious’ originated from 55 years at Cacak (44?°N, Serbia) and at Klein-Altendorf, University of Bonn (50?°N), cv. ‘Bobovec’ from the University of Ljubljana (46?°N, Slovenia) and cherry cv. ‘Burlat’ (35 years at Klein-Altendorf, 50?°N) and 20–30 years at Tuzla (44?°N, B & H) and Mostar (43?°N, Bosnia & Herzegovina; B & H).The annual precipitation showed a negligible change in the last 55 years of ±5% in the Balkan countries (e.?g. Cacak, 699?mm), but only ±1.8% at Klein-Altendorf (605?mm) with a slight shift to more winter rainfall.Regression analysis showed an increase in mean temperature of 1.7?°C (annual) and 1.45?°C for the vegetation period at Klein-Altendorf (last 55 years), Germany. In the evaluated Balkan stations, the greatest increase in annual air temperature between two climate phases (until 1987 versus from 1988 until now) was at Ljubljana (1.4?°C) and Cacak (1.2?°C) in contrast to the least increase (0.3?°C) at Tuzla (annual average 10?°C) and an intermediate value (0.9?°C) in the warmer Mostar (annual average 14.5?°C).This recent temperature increase advanced flower opening by 10–14 days for apple such as cv. ‘Golden Delicious’ at Cacak and 7–10 days (last 55 years) at Bonn and 4–5 days (last 35 years) for the early cherry cv. ‘Burlat’ at Klein-Altendorf/Bonn; at Mostar and Tuzla, the effect of climate change was greater with the early cherry cv. ‘Burlat’ (26 days) compared with the difference of the late cherry cultivar (16 days). Before recent climate change (1973–1987), cherry flower was advanced by 10 days in the warmer med climate at Mostar but delayed by 16 days in the cooler Tuzla. The results, i.?e. the flower advancement in the Continental climate but flower delay in the med climate, are discussed with respect to the risk of late frost and countermeasures to combat climate change effects.  相似文献