Balenine,Imidazole Dipeptide Promotes Skeletal Muscle Regeneration by Regulating Phagocytosis Properties of Immune Cells     

Min Yang  Luchuanyang Sun  Yasunosuke Kawabata  Fumihito Murayama  Takahiro Maegawa  Takeshi Nikawa  Katsuya Hirasaka 《Marine drugs》2022,20(5)

Balenine is one of the endogenous imidazole dipeptides derived from marine products. It is composed of beta-alanine and 3-methyl-L-histidine, which exist mainly in the muscles of marine organisms. The physiological functions of dietary balenine are not well-known. In this study, we investigated whether the supplementation of dietary balenine was associated with muscle function in a cardiotoxin-indued muscle degeneration/regeneration model. Through morphological observation, we found that the supplementation of balenine-enriched extract promoted the regeneration stage. In addition, the expression of regeneration-related myogenic marker genes, such as paired box protein 7, MyoD1, myogenin, and Myh3, in a group of mice fed a balenine-enriched extract diet was higher than that in a group fed a normal diet. Moreover, the supplementation of balenine-enriched extract promoted the expression of anti-inflammatory cytokines as well as pro-inflammatory cytokines at the degeneration stage. Interestingly, phagocytic activity in the balenine group was significantly higher than that in the control group in vitro. These results suggest that balenine may promote the progress of muscle regeneration by increasing the phagocytic activity of macrophages.  相似文献   

Response of the subterranean termite Coptotermes formosanus (Isoptera: Rhinotermitidae) to soil treated with microencapsulated fenobucarb     

Kubota S  Shono Y  Matsunaga T  Tsunoda K 《Pest management science》2007,63(12):1224-1229

BACKGROUND: Treating soil with insecticides is thought to be an effective method for termite control. Speed of action and repellency of insecticides are important factors for discussing their barrier effects as soil-treatment termiticides. The chemical barrier effect of microencapsulated fenobucarb against Coptotermes formosanus Shiraki was examined in the laboratory.RESULTS: Fenobucarb had a significantly faster lethal effect when applied to the mouthparts than to the tergites of worker termites. Soil that had been treated with > or = 50 mg kg(-1) of microencapsulated fenobucarb had a barrier effect within a single day, and could stop or retard the penetration of termites for 7 days. Microencapsulated fenobucarb did not act as a repellent, as indicated by a gradual increase in mortality at > or = 50 mg kg(-1) with slight penetration into the treated soil during the 7 day test.CONCLUSION: The results suggest that microencapsulated fenobucarb is a fast-acting termiticide, with a good barrier effect as a soil treatment, that also acts as a reduced repellent, retarding entry of termites into treated soil.  相似文献   

External transcutaneous ultrasound technique in the equine cricoarytenoideus dorsalis muscle: Assessment of muscle size and echogenicity with resting endoscopy     

Masato Satoh  Tohru Higuchi  Satoshi Inoue  Daisuke Miyakoshi  Ayako Kajihara  Tadahiro Gotoh  Yasuhito Shimizu 《Equine veterinary journal》2020,52(4):500-508

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Germination and growth inhibitors from wheat (Triticum aestivum L.) husks     

Kato T  Saito N  Kashimura K  Shinohara M  Kurahashi T  Taniguchi K 《Journal of agricultural and food chemistry》2002,50(22):6307-6312

On the basis of our findings that the germination of intact wheat seeds (with husks) belonging to dormancy varieties was restrained as compared with that of the dehusked seeds (grains), the germination inhibitors in the husks were explored. The water-soluble extracts from the husks were separated by the aid of inhibition assay experiments, resulting in the characterization of 2-phenylethyl alcohol 1, 4-vinylphenol 2 and its 2-methoxy derivative 3, and dihydroactinidiolide 4, all of which showed clear inhibition of germination at 500 ppm in aqueous solution. The related compounds 1-phenylethyl alcohol 5 and tetrahydroactinidiolide 6 were as active as 1 and 4, while no noticeable difference in activity was detected among both enantiomers and the DL-form of compounds 4-6. Clear synergistic relations were observed between 4 and 1 and also 4 and 3. Since the present inhibitors have been isolated from various kinds of seed plants, they may be responsible for the general germination inhibition in the seed plants.  相似文献   

An asymmetric energetic type Ic supernova viewed off-axis, and a link to gamma ray bursts     

Mazzali PA  Kawabata KS  Maeda K  Nomoto K  Filippenko AV  Ramirez-Ruiz E  Benetti S  Pian E  Deng J  Tominaga N  Ohyama Y  Iye M  Foley RJ  Matheson T  Wang L  Gal-Yam A 《Science (New York, N.Y.)》2005,308(5726):1284-1287

Type Ic supernovae, the explosions after the core collapse of massive stars that have previously lost their hydrogen and helium envelopes, are particularly interesting because of their link with long-duration gamma ray bursts. Although indications exist that these explosions are aspherical, direct evidence has been missing. Late-time observations of supernova SN 2003jd, a luminous type Ic supernova, provide such evidence. Recent Subaru and Keck spectra reveal double-peaked profiles in the nebular lines of neutral oxygen and magnesium. These profiles are different from those of known type Ic supernovae, with or without a gamma ray burst, and they can be understood if SN 2003jd was an aspherical axisymmetric explosion viewed from near the equatorial plane. If SN 2003jd was associated with a gamma ray burst, we missed the burst because it was pointing away from us.  相似文献   

Response of Wheat Grain to ABA and Imbibition at Low Temperature   总被引：1，自引：0，他引：1  

K. Noda    C. Kawabata  N. Kawakami 《Plant Breeding》1994,113(1):53-57

During after-ripening of wheat grains, embryo responsiveness to abscisic acid (ABA) decreases in parallel with the loss of grain dormancy. Dormant grains of Triticum aestivum L. line ‘Kitakei-1354’ that had lost almost half their dormancy due to chilling still respond to ABA like fully dormant grains. Imbibition a: low temperature appears to break the dormancy without change of embryo responsiveness to ABA. Gibberellin (GA) and ABA-regulated α-amylase synthesis of the embryos. This is amplified when the grains are imbibed at low temperature. Imbibition at low temperature appears to condition cells and tissues of the embryo for germination and α-amylase synthesis.  相似文献   

Detection of cyprinid herpesvirus-3 DNA in lake plankton     

Toshifumi Minamoto  Mie N. Honjo  Hiroki Yamanaka  Nobuyuki Tanaka  Tomoaki Itayama  Zen&#x;ichiro Kawabata 《Research in veterinary science》2011,90(3):530-532

The disease caused by cyprinid herpesvirus-3 (CyHV-3) severely impacts the natural freshwater ecosystem and damages carp and koi farming, however, the pathway of CyHV-3 transmission remains unclear. It is possible that the virus adheres to plankton, which then facilitate viral movement and transmission, and therefore, it is hypothesised that plankton are involved in the disease dynamics. In this study, plankton were collected at eight sites in the Iba-naiko lagoon; we detected and quantified CyHV-3 DNA from plankton samples. The results of the correlation analysis showed a significant positive correlation between CyHV-3 copies and the number of Rotifera, suggesting that CyHV-3 binds to and/or is concentrated by Rotifera. Our results suggest that plankton affect viral ecology in the natural environment.  相似文献   

Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan     

Itoh N  Itagaki T  Kawabata T  Konaka T  Muraoka N  Saeki H  Kanai K  Chikazawa S  Hori Y  Hoshi F  Higuchi S 《Veterinary parasitology》2011,176(1):74-78

The current study examined the prevalence of intestinal parasites and genotypes of Giardia intestinalis in puppies from nine pet shops in east Japan. Fresh fecal samples from 1794 puppies (≦3 months old) were collected on one occasion. Giardia spp. was examined for specific coproantigen using ELISA kit (SNAP^®Giardia, IDEXX Laboratories, Inc., USA). Other intestinal parasites were detected microscopically using the formalin-ethyl acetate sedimentation technique. Genotyping was determined for the random 29 stool samples identified as Giardia spp. positive using PCR and direct sequencing of the glutamate dehydrogenase (gdh) gene. Overall prevalence of protozoan Giardia spp. and Cystoisospora spp. revealed 23.4% and 11.3%, respectively. Prevalence of ascarids, Strongyloides spp. and hookworms were recorded 1.8%, 1.1% and 0.1%, respectively. Protozoan Giardia spp. and Cystoisospora spp., thus, represent important pathogens among pet shop puppies. All genotyped G. intestinalis isolates were belonged to assemblage C or D, identified as dog-specific genotypes. Zoonotic assemblage A and B were not demonstrated. The result suggests that the risk of zoonotic transmission of G. intestinalis from pet shops puppies to humans may be quite low in Japan.  相似文献   

Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300   总被引：1，自引：0，他引：1  

Nakashima K  Yanagisawa M  Arakawa H  Kimura N  Hisatsune T  Kawabata M  Miyazono K  Taga T 《Science (New York, N.Y.)》1999,284(5413):479-482

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Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid Todarodes pacificus and its application for elimination of bitterness from bitter peptides     

Tsuyoshi Komai  Choko Kawabata  Hiroaki Tojo  Shinobu Gocho  Eiji Ichishima 《Fisheries Science》2007,73(2):404-411

ABSTRACT:   An acidic serine carboxypeptidase (CPase Tpa) from the hepatopancreas of Japanese common squid Todarodes pacificus was purified. Purified CPase Tpa had a molecular mass of 36 kDa on sodium dodecylsulfate–polyacrylamide gel electrophoresis, and an isoelectric point of 6.0. The optimum pH of CPase Tpa was pH 4.0. In investigating the specificity of CPase Tpa for several peptide substances, it was found that peptides with hydrophobic or bulky amino acid residues at the P₁ position reacted well. The enzymatic activity was almost completely inhibited by p -chloromercuribenzoic acid, monoiodoacetic acid, diisopropylfluorophosphate and HgCl₂. This is the basis for its grouping in the serine carboxypeptidase family (EC 3. 4. 16. 5). The substrate specificity of CPase Tpa can be used to eliminate the bitterness of bitter peptides. In this study, the bitterness-reductive effect using bitter peptides prepared by hydrolyzing soy protein, casein and corn gluten with pepsin or trypsin was tested. The bitterness of soy peptide digested with pepsin was completely eliminated by treatment with CPase Tpa, whereas the bitterness of casein digested with trypsin and corn peptide digested with pepsin were somewhat less efficient. On the basis of these results, it is anticipated that CPase Tpa would be effective in eliminating the bitterness of some bitter peptides.  相似文献