排序方式： 共有96条查询结果，搜索用时 15 毫秒
61.
Mohammad Hossein Salehi Massoud Houshmand Omid Aryani Behnam Kamalidehghan Elham Khalili 《Iranian Biomedical Journal》2014,18(1):28-33
Background: Friedreich ataxia (FRDA) is an autosomal recessive disorder caused by guanine-adenine-adenine (GAA) triplet expansions in the FXN gene. Its product, frataxin, which severely reduces in FRDA patients, leads to oxidative damage in mitochondria. The purpose of this study was to evaluate the triple nucleotide repeated expansions in Iranian FRDA patients and to elucidate distinguishable FRDA clinical differences in these patients. Methods: A number of 22 Iranian patients (8 females and 14 males) from 16 unrelated families were studied. DNA was extracted from the peripheral blood of patients. The frequency and length of (GAA)n repeats in intron 1 of the FXN gene were analyzed using long-range PCR. In this study, the clinical criteria of FRDA in our patients and the variability in their clinical signs were also demonstrated. Results: An inverse relationship was observed between GAA repeat size and the age of onset. Although some distinguishable clinical features (such as limb ataxia and lower limb areflexia) were found in our patients, 90-95% of them had extensor plantar response and dysarthria. The results showed only one positive diabetes patient and also different effects on eye movement abnormality among our patients. Conclusion: The onset age of symptoms showed a significant inverse correlation with allele size in our patients (P>0.05). Based on comparisons of the clinical data of all patients, clinical presentation of FRDA in Iranian patients did not differ significantly from other FRDA patients previously reported. Key Words: Friedreich ataxia (FRDA), Frataxin, Mitochondria 相似文献
62.
Reza Salehi Abdolkarim Kashi Jung-Myung Lee Roghayeh Javanpour 《Journal of plant nutrition》2014,37(8):1255-1268
Macroelements in leaves and fruits, sugar of fruits, and yield of ‘Khatooni’ melon were compared with melons grafted onto rootstocks cvs. ‘Ace’, ‘Shintozwa’, and ‘ShintoHongto’, and trained into three methods: T1) no pinching and fruit thinning; T2) pinched to produce two lateral branches; and T3) pinched to two branches and all flowers and lateral branches from lower nodes thinned. Concentrations of nitrogen (N), phosphorus (P), and potassium (K) increased in leaves onto ‘Shintozwa’, whereas opposite trends observed for magnesium (Mg) and calcium (Ca). N, P, and K in leaves were significantly higher by 21.5%, 17.2%, and 18.6%, respectively in grafted. N, P and K in fruits onto ‘Ace’ and ‘Shintozwa’ were higher, with exception of Ca and Mg in non-grafted. ‘Ace’ and ‘Shintozwa’ resulted in significant increase in Khatooni yield. T3 showed the highest yield. Soluble solids concentration (SSC) was higher in non-grafted fruits. T2 produced fruits with the highest SSC. 相似文献
63.
Leila Pirmoradi Mohammad Taghi Mohammadi Akbar Safaei Fakhardin Mesbah Gholam Abbas Dehghani 《Iranian Biomedical Journal》2014,18(3):173-180
Background: Data shows vanadium protects pancreatic beta cells (BC) from diabetic animals. Whether this effect is direct or through the relief of glucose toxicity is not clear. This study evaluated the potential effect of oral vanadyl sulfate (vanadium) on glycemic status and pancreatic BC of normal and diabetic rats. Methods: Rats were divided into five groups of normal and diabetic. Diabetes was induced with streptozocin (40 mg/kg, i.v.). Normal rats used water (CN) or vanadium (1 mg/ml VOSO4, VTN). Diabetic rats used water (CD), water plus daily neutral protamine Hagedorn insulin injection (80 U/kg, ITD) or vanadium (VTD). Blood samples were taken for blood glucose (BG, mg/dL) and insulin (ng/dL) measurements. After two months, the pancreata of sacrificed rats were prepared for islet staining. Results: Pre-treated normal BG was 88 ± 2, and diabetic BG was 395 ± 9. The final BG in CD, VTD, and ITD was 509 ± 22, 138 ± 14, and 141 ± 14, respectively. Insulin in VTN (0.75 ± 0.01) and VTD (0.78 ± 0.01) was similar, higher than CD (0.51 ± 0.07) but lower than CN (2.51 ± 0.02). VTN islets compared to CN had larger size and denser central core insulin immunoreactivity with plentiful BC. CD and ITD islets were atrophied and had scattered insulin immunoreactivity spots and low BC mass. VTD islets were almost similar to CN. Conclusion: Besides insulin-like activity, vanadium protected pancreatic islet BC, and the relief of glucose toxicity happening with vanadium had a little role in this action. Key Words: Vanadium, Rats, Diabetes, Protection, Beta cells 相似文献
64.
Babak Mamnoon Taghi Naserpour Farivar Ahmad Reza Kamyab Dariush Ilghari Ali Khamesipour Mohsen Karimi Arzenani 《Iranian Biomedical Journal》2016,20(1):56-62
Background:
Existence of bacterial host-cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor intensive and rather expensive. In this study, a rapid real-time PCR test was served as a method of choice to quantify the E. coli host- cell DNA contamination in widely used recombinant streptokinase (rSK), and alpha interferon (IFN-α) preparations.Methods:
A specific primer pair was designed to amplify a sequence inside the E. coli 16S rRNA gene. Serial dilutions of DNA extracted from E. coli host cells, along with DNA extracted from Active Pharmaceutical Ingredients of rSK, and IFN-α samples were subjected to an optimized real-time PCR assay based on SYBR Green chemistry.Results:
The test enabled us to detect a small quantity of genomic DNA contamination as low as 0.0002 pg in recombinant protein-based drugs. For the first time, this study showed that DNA contamination in rSK and IFN-α preparation manufactured in Pasteur Institute of Iran is much lower than the safety limit suggested by the US FDA.Conclusion:
Real-time PCR is a reliable test for rapid detection of host-cell DNA contamination, which is a major impurity of therapeutic recombinant proteins to keep manufacturers’ minds on refining drugs, and provides consumers with safer biopharmaceuticals. Key Words: DNA contamination, Real-time PCR, Streptokinase, Interferon-alpha (IFN-α) 相似文献65.
66.
Y. Emam A. Shekoofa F. Salehi A. H. Jalali 《Archives of Agronomy and Soil Science》2013,59(5):527-534
In most rainfed production areas where wheat fallow is common, crop yield could be improved by changing to more diverse crop rotations. It seems necessary to identify crop cultivars suitable for such diversification. This study was aimed at determining the responses of two common bean cultivars with different growth habits (Sayyad as an indeterminate and D81083 as a determinate cultivar) to drought stress. The study was conducted in a greenhouse during the 2008 growing season. A factorial experiment in a randomized complete block design with four replications and four water levels [100, 75, 50 and 25% of field capacity (FC) by weight] was used. The results showed that plant height, number of leaves, leaf area, number of pods, pod dry matter (DM) and total plant DM weights of both cultivars were significantly reduced under drought stress. Furthermore, at 50 and 25% FC, all plant pods of both cultivars were aborted. The common bean cultivar with a determinate growth habit appeared to have potential as a dryland rotation crop for farming in arid regions. Further field research might shed more light on the sensitivity of bean cultivars to drought stress with the aim of crop diversification for dryland areas, where adequate moisture supply is a limiting factor for crop production. 相似文献
67.
Ghanbarpour Reza Sami Masoud Salehi Mahmood Ouromiei Mahla 《Tropical animal health and production》2011,43(1):153-157
The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in
to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic
and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence
genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence
of stx
2
gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and
eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence
genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected
in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx
1
virulence gene sequences. 相似文献
68.
Mohammad Taghi Feyzbakhsh Behnam Kamkar Hassan Mokhtarpour Mohammad Esmaeil Asadi 《Archives of Agronomy and Soil Science》2013,59(11):1581-1592
A 2-year field experiment (2012–2013) was conducted to evaluate the yield and water use efficiency (WUE) response of maize (Zea mays L.) to different soil water managements at different sowing dates. The experiment included three sowing dates (22 June, 6 July and 21 July) and four irrigation regimes based on maximum allowable depletion (MAD) of the total available soil water (TAW). The irrigation treatments were marked by I1 to I3 as 40%, 60% and 80% MAD of TAW, respectively, and with no irrigation. The results showed that grain yield reduced when planting was delayed in both years, ranging from 6105 to 4577 kg ha?1 in 2012 and from 7079 to 5380 kg ha?1 in 2013. However, WUE increased when planting was delayed from 22 June until 21 July. Also the highest grain yield was observed in the first irrigation treatment (MAD = 40%) in both years, and the highest WUE was obtained in the second irrigation treatment (MAD = 60%) with 1.64 and 1.61 (kg m?3) in 2012 and 2013, respectively. These findings suggest that delay in planting date and the use of MAD = 60% treatment in Mediterranean-type region such as Golestan, Iran, can be useful in saving water that is highly important in such regions. 相似文献
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