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71.
The immunodominant membrane protein Imp of several phytoplasmas within the ‘Candidatus Phytoplasma aurantifolia’ (16Sr‐II) group was investigated. Eighteen isolates from Iran (11), East Asia (5), Africa (1) and Australia (1) clustered into three phylogenetic subgroups (A, B and C) based on the 16S rDNA and imp genes, regardless of geographic origin. The imp gene sequences were variable, with more non‐synonymous than synonymous mutations (68 vs 20, respectively), even though many of the non‐synonymous ones (75%) produced conservative amino acid replacements. Eight codon sites on the extracellular region of the protein were under positive selection, with most of them (75%) coding for non‐conservative amino acid substitutions. Full‐length (21 kDa) and truncated (16 kDa) Imp proteins of two economically important Iranian phytoplasmas [lime witches’ broom (LWB) and alfalfa witches’ broom (AlWB‐F)] were expressed as His‐tagged recombinant proteins in Escherichia coli. An antiserum raised against full‐length recombinant LWB Imp reacted in western blots with membrane proteins extracted from LWB‐infected periwinkle and lime, indicating that Imp (19 kDa) is expressed in infected plants and is a membrane‐associated protein. The same polyclonal antibody also detected native Imp in proteins from periwinkles infected by phytoplasmas closely related to LWB (subgroup C) only, confirming phylogenetic clustering based on 16S rDNA and imp genes. Imp proteins of LWB and AlWB‐F isolates were also recognized by an antiserum raised against an enriched preparation of AlWB‐F phytoplasma cells, demonstrating the antigenic properties of this protein. 相似文献
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Saeed Khazaie Mahvash Jafari Javad Heydari Alireza Asgari Kavoos Tahmasebi Maryam Salehi Mohammad Saleh Abedini 《Journal of animal physiology and animal nutrition》2019,103(5):1619-1628
Diazinon (DZN) as an organophosphate pesticide may cause oxidative stress in different tissues. Antioxidants increase tissue protection from oxidative stress. The aim of the present study was to investigate prophylactic and therapeutic effects of vitamin C against oxidative stress caused by DZN in various tissues of male Wistar rats. Thirty rats were divided into five groups: control group received corn oil as DZN solvent, DZN group received 100 mg/kg of DZN, C group received 200 mg/kg of vitamin C, C‐DZN and DZN‐C groups received vitamin C before and after DZN injection. Plasma and various tissues were prepared and evaluated for measurement of the biochemical parameters and oxidative stress biomarkers. Results showed that acute administration of DZN significantly increased superoxide dismutase and glutathione‐S‐transferase activities and malondialdehyde level in all tissues, catalase (CAT) activity in liver, kidney and heart and some plasma biochemical indices, while it decreased cholinesterase and lactate dehydrogenase activities and glutathione content in all tissues. CAT activity in erythrocytes, brain and spleen was decreased in DZN‐exposed rats compared with the control group. Administration of vitamins C in both prophylactic and therapeutic groups ameliorated in these parameters, although all these tests in tissues did not return to the normal level. These data suggest that oxidative stress is an essential mechanism involved in DZN‐induced adversity effect, as evidenced by the altered activity of antioxidant enzymes, depleted GSH content and the enhanced membrane lipid peroxidation. Both the prophylactic and therapeutic treatments of rats to vitamin C have beneficial effects against oxidative stress and cholinergic hyperactivity induced by DZN in tissues especially in the brain tissue through free radical scavenging. 相似文献
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Amaneh Mohammadi Roushandeh Iraj Salehi Motahareh Mortazavi 《Iranian Biomedical Journal》2015,19(2):96-101
Background:
A high-fat diet (HFD) promotes the oxidative stress formation, which in turn has hazardous effects on reproductive system and fertility. The present study examines the potential positive effects of a restricted high-fat diet (RHFD) and antioxidants consumption on sperm parameters and testis tissue in rats.Methods:
Male rats (n = 48) were divided into four groups (12 in each group): control group (Cont), HFD group, RHFD, and RHFD with astaxanthin and vitamins E and C group (RHFDA). After 12 weeks, serum analysis and sperm parameters study were performed. Sections of fixed testes were stained with Hematoxilin and Eosin to study the histological changes. A one-way ANOVA was used to compare the data.Results:
HFD fed animals presented significant increase in weight load and serum low density lipoprotein (LDL-C) levels (P < 0.05). The sperm count in RHFD was lower than three other groups (P < 0.05) and sperm motility of RHFDA group was significantly higher than HFD and RHFD groups (P < 0.05). The histological study was showed a significant increase in spermatogonium number in RHFDA compared to three other groups (P < 0.05). The number of spermatocyte I and spermatid in RHFD was significantly (P < 0.05) lower than Cont and HFD groups.Conclusion:
HFD and obesity can affect sperm parameters and spermatogenesis and antioxidants consumption may improve their quality. Although the RHFD is a benefit way in weight loss and decrease of LDL-C of serum, but it is suggested that is not effective on sperm quality improvement.Key Words: Spermatogenesis, high-fat Diet, Restricted fat diet, Antioxidants, Astaxanthin 相似文献76.
A. Shojaee Tabrizi Sh. Jamshidi A. Oghalaei T. Zahraei Salehi A. Bayati Eshkaftaki M. Mohammadi 《Veterinary microbiology》2010,140(1-2):142-146
The definite mode of transmission of Helicobacter infection is largely unknown. This study was carried out primarily, to determine the existence of Helicobacter spp. in the oral secretions of stray cats as one of the possible routes of transmission and secondly, to evaluate the accordance between oral and gastric colonization of Helicobacter spp. in these cats. Forty-three adult stray cats were thus studied for the presence of Helicobacter species by quantitative rapid urease test (RUT), cytology and PCR. Helicobacter spp. were found in the oral secretions and gastric biopsies of 93% and 67.5% of the stray cats, respectively. There was not, however, any agreement observed between Helicobacter colonization at these two locations, at neither genus nor species level. These findings suggest that the oral cavity is routinely exposed to transient forms of bacteria and may temporarily harbor Helicobacter spp. Thus, oral cavity as a source of Helicobacter spp. may act as a reservoir for transmission and may not necessarily reflect the colonization status of the gastric mucosa. 相似文献
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Hamed Zamankhan Malayeri Shahram Jamshidi Taghi Zahraei Salehi 《Veterinary research communications》2010,34(5):435-444
Bacterial agents are considered important pathogens causing external otitis in dogs. It is essential to carry out bacterial
culture and antimicrobial susceptibility test in the case of otitis externa, particularly for chronic or recurring cases.
Sterile swab samples were obtained from terminal part of vertical ear canals of 74 dogs with otitis externa for cytology,
bacterial culture and antimicrobial susceptibility test. Cytologic smears were stained using Gram and Giemsa staining methods.
Aerobic bacterial culture performed on blood agar and MacConkey agar. Among total number of 92 isolated bacteria, 68 were
Staphylococcus intermedius. Other isolated bacteria included: Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Pasteurella canis, and six other species of coagulase-negative Staphylococcus. Antimicrobial susceptibility test were performed for all isolated bacteria using 14 antibiotics. Based on the results of
this study, all isolated Staphylococcus spp. were sensitive to amikacin, enrofloxacin, and rifampin, and had low resistance to gentamicin, cephalothin and ceftriaxone.
More than half of gram-positive isolates were resistant to penicillin and ampicillin. Generally, all isolated gram-negative
bacteria, were sensitive to amikacin and enrofloxacin, and had low resistance to ceftriaxone and gentamicin. They were highly
resistant to penicillin, eythromycin, and cephalothin. Regarding the results of this study, in cases of uncomplicated otitis
externa, it is possible to select antimicrobial drugs merely based on cytology, but it is recommended to perform bacterial
culture and antimicrobial susceptibility test. However, in complicated or refractory cases, antimicrobials should be selected
based on bacterial culture and antimicrobial susceptibility test. 相似文献
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This study was conducted to characterize the Escherichia coli isolates from colisepticemic Japanese quails. One hundred and nine E. coli were isolated in pure culture from heart blood of dead Japanese quails. The sampled birds were originated from four different
farms. Antibiotic resistance pattern of E. coli isolates were determined against nine antibacterial agents. Phylotype and virulence genes of the isolates were detected by
polymerase chain reaction. By disc diffusion method, all of the isolates showed resistance to three or more antibiotics, and
19 different patterns of multiple drug resistance were observed. Phylotyping of the most prevalent multiple drug-resistant
isolates revealed that they mostly belonged to phylogroups A (A1 subgroup). The E. coli isolates belong to four phylogenetic groups: A (55.0%), B1 (18.3%), B2 (17.4%), and D (9.2%). Eighty-nine (81.7%) isolates
were distributed in five phylogenetic subgroups including 22 (20.2%) in A0, 38 (34.9%) in A1, 19 (17.4%) in B23, 7 (6.4%) in D1, and 3 (2.8%) in D2. The examined E. coli isolates exhibit at least one of the virulence genes tested, whereas three most prevalent genes were crl (94.5%), fimH (89.0%), and iutA (51.4%), respectively. The genetic marker for Afa (afaI B-C), S (sfa/focD-E), and P (papE-F) fimbriae were found in one, four, and ten isolates, respectively. Thirteen different combinations of virulence gene were
observed, where combination of crl and fimH genes was the most prevalent pattern. None of the isolates contained the ipaH, stx1, stx2, and eaeA genetic markers. In conclusion, E. coli strains could be considered as a causative agent of mortality in quail farms. In conclusion, E. coli isolates from colisepticemic quails are distributed in different phylogroups, are resistant to combinations of antibiotic
agents, and contain several virulence genes. 相似文献
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Shokufeh Salehi Khoshkroudi Mirkhalegh Ziatabar Ahmadi Meysam Ramezani 《Archives of Agronomy and Soil Science》2013,59(8):1155-1172
The measurement of saturated water content (SWC) is necessary in the estimation of soil water retention and unsaturated hydraulic conductivity curves. In several studies, pedotransfer functions (PTFs) were developed to predict SWC. Among them, evolutionary polynomial regression (EPR) is one that can operate on large quantities of data in order to capture nonlinear and complex interactions between the variables of the system. In this study, the evolutionary data-mining technique was used to derive new PTFs and different methods were evaluated, such as the soil porosity method, Rosetta method, and others, for the estimation of SWC. For this purpose, 270 soil samples (3:1 ratio for development and validation) from three data sets were used. Among 190 PTFs provided by EPR, one equation with the highest accuracy and the least number of inputs was selected. The EPR predictions were compared with the experimental results as well as the PTFs proposed in previous studies. Comparison of the statistical indicators showed that the ‘proposed PTF’ and ‘porosity method’ are the best and worst methods for the prediction of SWC, respectively. Also, good predictions were achieved from the proposed approaches by the groups of Scheinost, Vereecken, and Williams. 相似文献