Cabbage pollen germination and longevity     

M. S. Chiang 《Euphytica》1974,23(3):579-584

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Studies on glucosinolates in clubroot resistant selections and susceptible commercial cultivars of cabbages     

Calvin Chong  M. S. Chiang  R. Crete 《Euphytica》1985,34(1):65-73

Summary Heads of 59 commercial cabbage cultivars, all susceptible to clubroot disease, and of 86 individual clubroot resistant cabbage plants of various breeding selections were analysed for their composition in glucosinolates, determined by quantifying their hydrolytic breakdown products, thiocyanate, goitrin, and volatile isothiocyanates. The mean thiocynate ion content was significantly lower in the breeding selections (120 g/g dry weight) than in the commercial cultivars (204 g/g). In contrast, the mean goitrin content was significantly higher in the breeding selections (193 g/g) than in the commercial cultivars (35 g/g). Similar to goitrin, the range of volatile isothiocyanates and total glucosinolate were higher in the breeding selections, but the mean contents of each were not statistically different between selections and cultivars. Fourteen cultivars and four selections were found to be free of goitrin; three cultivars, but no breeding selection were free of volatile isothiocyanates. The breeding selections will provide germplasm for breeding new clubroot resistant and low glucosinolate cultivars.Contribution No. J-965.  相似文献   

Rapid screening of wood chemical component variations using transmittance near-infrared spectroscopy     

Yeh TF  Yamada T  Capanema E  Chang HM  Chiang V  Kadla JF 《Journal of agricultural and food chemistry》2005,53(9):3328-3332

A rapid transmittance near-infrared (NIR) spectroscopy method was developed to predict the variation in chemical composition of solid wood. The effect of sample preparation, sample quantity (single versus stacked multiple wood wafers), and NIR acquisition time on the quantification of alpha-cellulose and lignin content was investigated. Strong correlations were obtained between laboratory wet chemistry values and the NIR-predicted values. In addition to the experimental protocol and method development, improvements in calibration error associated with utilizing stacked multiple wood wafers as opposed to single wood wafers are also discussed.  相似文献   

Antimutagenic constituents of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) with potential cancer chemopreventive activity     

Chen HH  Chiang W  Chang JY  Chien YL  Lee CK  Liu KJ  Cheng YT  Chen TF  Kuo YH  Kuo CC 《Journal of agricultural and food chemistry》2011,59(12):6444-6452

Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans-coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation.  相似文献   

A glucose-insensitive T7 expression system for fully-induced expression of proteins at a subsaturating level of L-arabinose     

Wang ZW  Lai CB  Chang CH  Chiang CJ  Chao YP 《Journal of agricultural and food chemistry》2011,59(12):6534-6542

The L-arabinose (Ara)-controlled T7 expression system was previously constructed by creation of an Escherichia coli BL21(BAD) strain. The production of recombinant proteins in this strain was stringently regulated and reached a high level upon induction with Ara. Nevertheless, this system is still associated with inherent problems of interference with glucose and of the all-or-nothing induction profile at a subsaturating level of Ara. In this study, these problems were circumvented by modifying the physiological traits of BL21(BAD) strain. This was followed by deletion of ptsG gene and the araFGH and araBAD operon. The former encodes the glucose transporter while the latter two gene operons produce proteins responsible for Ara uptake and catabolism. In addition, the expression of genomic araE (encodes the Ara transporter) was constitutively enhanced. The resulting strain was designated BAD-5. By expression of the faster degrader GFP(LAA) at a subsaturating level of Ara, 80% of BAD-5 strain was found visually bright in the presence or absence of glucose. A further analysis by flow cytometry showed a uniform distribution of GFP expression for BAD-5 strain. In marked contrast, BL21(BAD) strain exhibiting visual brightness was less than 10% of the cell population and remained dark in the presence of glucose. Moreover, a saturated level of luciferase from Renilla reniformis (Rluc) could be readily obtained in BAD-5 strain at 20 μM Ara regardless of glucose. Rluc in BL21(BAD) strain was produced in an Ara dose-dependent manner, and the protein production became arrested when glucose was present. Overall, it illustrates the usefulness of the improved system for overproduction of recombinant proteins in an efficient, homogeneous, and glucose-insensitive way.  相似文献   

Starch characteristics and their influences on in vitro and pig prececal starch digestion     

Lee TT  Huang YF  Chiang CC  Chung TK  Chiou PW  Yu B 《Journal of agricultural and food chemistry》2011,59(13):7353-7359

The main objective of this research was to study the characteristics of starch granules and their influences on in vitro and pig prececal starch digestion of corn, dehulled barley, wheat, and potato. Scanning electron microscopy was used to study the starch endosperm structure in the parent material as well as in vitro starch digestion. The results showed that corn starch granules were polyhedral, with a diameter ranging from 2 to 10 μm, whereas those of dehulled barley and wheat were spherical, with a diameter ranging from 5 to 20 μm. Potato had the largest starch granules among starch sources reported herein, with oval spheres of 10-50 μm in diameter. In vitro starch hydrolysis showed that starch granules of corn degraded faster than the starch of dehulled barley and wheat, with the potato starch being degraded the slowest. The in vivo digestibility trial using ileal-cannulated pigs confirmed the starch degradation of grains. The in vitro (x, %) and in vivo (y, %) digestibility were highly correlated [y = 6.5304x - 538.48 (R(2) = 0.9924)]. On the basis of the results, in vitro starch hydrolysis might be useful in predicting in vivo prececal starch digestibility. The digestion kinetic characteristics of different starch sources might be employed to evaluate the starch digestive rate at the pig ileum.  相似文献   

Antiosteoporotic effects of Lactobacillus -fermented soy skim milk on bone mineral density and the microstructure of femoral bone in ovariectomized mice     

Chiang SS  Pan TM 《Journal of agricultural and food chemistry》2011,59(14):7734-7742

Osteoporosis is a major skeletal disease associated with loss of estrogen in postmenopausal women. In this study, Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101F) and Lactobacillus plantarum NTU 102 (NTU 102F) were used as starters to ferment soy skim milk. This was then used as a nutritional supplement for 8 weeks to ovariectomized (OVX) mice. This study reveals that soy skim milk fermented with lactobacilli can increase the contents of aglycone isoflavones, soluble calcium, and vitamin D(3). The trabecular bone volumes and trabecular number of the distal femur in mice fed NTU 101F increased by a factor of 1.48 and 1.74 compared with the OVX group. The bone network density and thickness of the distal metaphyseal trabecular in mice fed NTU 101F and Fosamax was significantly greater than that of OVX mice. These results suggest that fermented soy skim milk can attenuate bone loss in OVX mice and lower the risk of osteoporosis.  相似文献   

Synthesis and Rheological Characterization of Water-Soluble Glycidyltrimethylammonium-Chitosan     

Syang-Peng Rwei  Yu-Ming Chen  Wen-Yan Lin  Whe-Yi Chiang 《Marine drugs》2014,12(11):5547-5562

In this study, chitosan (CS) grafted by glycidyltrimethylammonium chloride (GTMAC) to form GTMAC-CS was synthesized, chemically identified, and rheologically characterized. The Maxwell Model can be applied to closely simulate the dynamic rheological performance of the chitosan and the GTMAC-CS solutions, revealing a single relaxation time pertains to both systems. The crossover point of G′ and Gʺ shifted toward lower frequencies as the CS concentration increased but remained almost constant frequencies as the GTMAC-CS concentration increased, indicating the solubility of GTMAC-CS in water is good enough to diminish influence from the interaction among polymer chains so as to ensure the relaxation time is independent of the concentration. A frequency–concentration superposition master curve of the CS and GTMAC-CS solutions was subsequently proposed and well fitted with the experimental results. Finally, the sol-gel transition of CS is 8.5 weight % (wt %), while that of GTMAC-CS is 20 wt %, reconfirming the excellent water solubility of the latter.  相似文献   

Oral challenge of brushtail possums (Trichosurus vulpecula) with possum enteroviruses: Clinical observation,antibody response and virus excretion in faeces     

T Zheng  CHM Chiang 《New Zealand veterinary journal》2013,61(5):217-221

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Impact of climate change on paddy field irrigation in southern Taiwan   总被引：1，自引：1，他引：0  

Jie-Lun Chiang  Tzu-Ming Liu 《Paddy and Water Environment》2013,11(1-4):311-320

Climate change can have a serious impact on water resources. The main agricultural product in southern Taiwan is rice, the planting of which consumes far more water than other crops. This makes agriculture in Taiwan especially vulnerable to climate change. In this study, we used the generalized watershed loading functions (GWLF) hydrological model to simulate the discharge of the Kaoping River under climate change scenarios A2 and B2 as released by the Intergovernmental Panel on Climate Change. We discussed the potential impact of climate change on water resources based on the results of GWLF simulations carried out using rainfall and temperature data from five general circulation models (GCMs). The simulation results indicate that river discharge in the wet season increases significantly, and decreases in the dry season. The discharge variations from using the various GCMs as inputs fall within the range of ?26 to +15 % for the dry season and ?10 to +82 % for the wet season. The variation in available water will seriously impact the first period rice farming (the period between the beginning of January and the end of May) in southern Taiwan. Consequently, effective reduction in conveyance loss in the irrigation canal systems and proper fallowing of paddy fields will be the main challenges to Taiwan’s agricultural sector for alleviating the impact of climate change. For further decision making, we show the effects of adapting to climate change by various degrees of the following two methods: fallowing paddy fields to various degrees and reducing conveyance loss in irrigation canal systems.  相似文献