Effect of mitotane on pituitary corticotrophs in clinically normal dogs     

Taoda T  Hara Y  Takekoshi S  Itoh J  Teramoto A  Osamura RY  Tagawa M 《American journal of veterinary research》2006,67(8):1385-1394

OBJECTIVE: To evaluate the effects of mitotane administration on the function and morphology of pituitary corticotrophs in clinically normal dogs. ANIMALS: 12 clinically normal adult Beagles. PROCEDURES: Dogs were randomly assigned to the control group or the mitotane treatment group. In mitotane treatment group dogs, mitotane was administered for 1 month. In both groups, ACTH stimulation testing and corticotrophin-releasing hormone (CRH) stimulation testing were performed. Magnetic resonance imaging (MRI) of the pituitary gland and brain was performed in mitotane treatment group dogs before and after administration of mitotane. After CRH stimulation testing and MRI, dogs were euthanatized and the pituitary gland and adrenal glands were excised for gross and histologic examination. RESULTS: ACTH concentrations in mitotane treatment group dogs were significantly higher than in the control group dogs following CRH stimulation. Magnetic resonance imaging revealed that pituitary glands were significantly larger in treatment group dogs after administration of mitotane, compared with before administration. On gross and histologic examinations, the adrenal cortex was markedly atrophied. Immunohistochemistry revealed hypertrophy of corticotrophs in pituitary glands of mitotane treatment group dogs. CONCLUSIONS AND CLINICAL RELEVANCE: These findings indicate that inhibition of the adrenal cortex by continuous administration of mitotane leads to functional amplification and morphologic enhancement of corticotrophs in clinically normal dogs. In instances of corticotroph adenoma, hypertrophy of individual corticotrophs induced by mitotane may greatly facilitate enlargement of the pituitary gland and increases in ACTH secretion.  相似文献   

Geographic variability in taxonomic composition,standing stock,and productivity of the mesozooplankton community around the Kuroshio Current in the East China Sea          下载免费PDF全文

Toru Kobari  Wataru Makihara  Takahiro Kawafuchi  Kie Sato  Gen Kume 《Fisheries Oceanography》2018,27(4):336-350

Despite the low productivity that has been thought to characterize plankton communities in the western boundary current of the North Pacific Subtropical Gyre, many migratory fishes risk encountering low food availability during crucial life history stages by reproducing and recruiting in the Kuroshio region (i.e., the Kuroshio Paradox). Here, we report on geographic variability in taxonomic composition, biomass, and productivity of the mesozooplankton community in the Kuroshio Current and neighboring waters in the East China Sea. Calanoid copepods were the most abundant mesozooplankton taxon throughout our sampling stations. Small copepods, which include nauplii and poecilostomatoids, and gelatinous metazoans were the next most abundant. Seasonal variability in mesozooplankton standing stock (i.e., abundance and biomass) and productivity (i.e., production rate and protein synthetase activity) exceeded spatial variability across the stations and regions. The mesozooplankton community was characterized by high biomass and production rates in the summer, as well as high abundance and protein synthetase activity in the fall. No significant differences were found for mesozooplankton standing stock and productivity in the Kuroshio Path relative to those on the continental shelf or on the outside of the Kuroshio Path. Our results indicate that the standing stock and productivity of the mesozooplankton community in the Kuroshio Path are equivalent to those on the continental shelf, and that these communities are supported by small copepods and gelatinous zooplankton. We suggest that the mesozooplankton standing stocks and productivity provide adequate food availability for migratory fishes in the Kuroshio and neighboring waters in the East China Sea.  相似文献   

Estimation of nutrient excretion factors of broiler and layer chickens in Japan          下载免费PDF全文

Akifumi Ogino  Hitoshi Murakami  Takahiro Yamashita  Motohiro Furuya  Hirofumi Kawahara  Takako Ohkubo  Takashi Osada 《Animal Science Journal》2017,88(4):659-668

We estimated the nitrogen (N), phosphorus (P), potassium (K) and magnesium (Mg) excretion factors of broiler and layer chickens in Japan, using two approaches and the latest data available. In the top‐down approach, we determined the nutrient amounts in the feeds and those in the products (i.e. the liveweight gain, eggs), and the national nutrient excretions were determined as the difference between these amounts. We then calculated the nutrient excretion factors by dividing the national excretions by the number of animals. In the bottom‐up approach, we calculated the amounts of nutrients in the feed and product per head using productivity parameters (feed conversion ratio, etc.). The differences between these amounts were considered the nutrient excretion factors. The average nutrient excretion factors of broilers (g/day/head) estimated using the top‐down and bottom‐up approaches were: N, 1.40 and 1.87; P, 0.36, 0.50; K, 0.54, 0.77; Mg, 0.13, 0.18, respectively. The excretion factors obtained by the top‐down approach can be used to calculate the national/regional excretions. The two approaches resulted in almost the same excretion factors for layers, and the average nutrient excretion factors of layers (g/day/head) estimated were: N, 2.20; P, 0.55; K, 0.68; Mg, 0.23. The estimated excretion factors for N (only) are smaller than the reported factors.  相似文献   

Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips     

Yoshio KIKU  Yuya NAGASAWA  Kazue SUGAWARA  Takahiro YABUSAKI  Kazuyoshi OONO  Kento FUJII  Koji MAEHANA  Tomohito HAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1628

The accurate identification of mastitis‐causing bacteria assists in effective management by both dairy farmers and veterinarians and can be used to implement the selective use of antimicrobials for treatment. The purpose of this study was to evaluate the ability of our developed anti–ribosomal protein-L7/L12 antibody–coated immunochromatographic strip (ICS) test to detect coliforms in milk by comparing the results with the bacteriological culture method. We investigated the performance of the ICS test as compared with the bacteriological culture method using 308 milk samples from clinical bovine mastitis. First, to determine the optimal ICS test cutoff point for detecting coliform mastitis, we developed a receiver-operating characteristic curve. The result showed that the cutoff point was at 0.5 of our index. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value of the ICS test were 81.3%, 84.8%, 69.2%, and 91.54%, respectively. As the clinical signs increased in severity, the F-measure, a weighted harmonic mean of the sensitivity and overall PPV performance, increased. Because it is especially important to treat clinical mastitis appropriately in the early stages of detection, the ICS test, which can be used by both dairy farmers and veterinarians on dairy farms, is considered to be a useful tool for detecting coliform mastitis, which often presents with severe signs.  相似文献   

Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study     

Akira GOTO  Masaaki TAGAMI  Fumiki KATO  Tsukasa SUZUKI  Takashi YAMAGA  Harutaka MURASE  Fumio SATO  Munkhtuul TSOGTGEREL  Tadamasa NIIKURA  Tomoe MORIYAMA  Akiko CHIBA  Ken-ichi WATANABE  Nao TSUZUKI  Yasuo NAMBO 《Journal of Equine Science》2021,32(4):147

We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares.  相似文献   

Metallic lustre and the optical mechanism generated from the leaf surface of Begonia rex Putz.     

Yang Zhang  Takahiro Hayashi  Munetaka Hosokawa  Susumu Yazawa  Yuhua Li 《Scientia Horticulturae》2009

The mechanism of sparkling metallic lustre formation in leaves of Begonia rex Putz. was investigated. On the metallic lustre and normal green areas of one leaf, we observed two light reflection patterns, one a spotted pattern (SP) composed of white spots formed on the centre of epidermal cells and the other a polygonal pattern (PP) composed of white polygons formed around the epidermal cell edges. The SP was observed both in metallic lustre and normal green areas, and the intensity did not differ significantly, but the PP was much stronger in the metallic lustre area. Different reflection of the PP in metallic lustre and normal green areas indicated that metallic lustre formation was attributable to the PP. Light reflection of the PP was mainly derived from interior light reflection because the PP became very weak after air removal from leaf. The formation of the PP is associated with the interior structure of the leaf, such as chlorophyll density, cell arrangement and air space. The experimental results indicated that interior air space is an important factor in forming the polygonal pattern.  相似文献   

Relationship Between Physicochemical Properties of Starches and White Salted Noodle Quality in Japanese Wheat Flours     

Takahiro Noda  Takuji Tohnooka  Shozo Taya  Ikuo Suda 《Cereal Chemistry》2001,78(4):395-399

This study describes the effect of starch properties of Japanese wheat flours on the quality of white salted noodles (WSN). Starch was isolated from 24 flours of 17 Japanese cultivars and amylose content was determined along with pasting properties by Rapid Visco Analyser (RVA), thermal properties by differential scanning calorimetry (DSC), and the distribution of amylopectin chain length by high‐performance anion exchange chromatography (HPAEC). Twenty flours were used to prepare WSN. As expected, 5–6% lower amylose content was associated with good WSN quality (higher scores in softness, elasticity, and smoothness). RVA analysis indicated that the pasting temperature had the greatest influence on WSN quality, while breakdown and setback showed slight effects on WSN quality. DSC results showed that lower endothermal enthalpy (ΔH) in the amylose‐lipid complex was associated with good WSN quality. Chainlength distribution of amylopectin by HPAEC was not an important factor in relation to WSN quality.  相似文献   

Properties of Starches from Several Low‐Amylose Rice Cultivars     

Takahiro Noda  Yoichi Nishiba  Tetsuo Sato  Ikuo Suda 《Cereal Chemistry》2003,80(2):193-197

The starch properties of five low‐amylose rice cultivars, Yawarakomachi, Soft 158, Hanabusa, Aya, and Snow Pearl, were compared with those of two normal amylose rice cultivars, Nipponbare and Hinohikari. There were no large differences in the distributions of the amylopectin chain length determined by high‐performance anion‐exchange chromatography, and the starch gelatinization properties determined by differential scanning calorimetry, between normal and low‐amylose rice cultivars. Results obtained using rapid viscosity analysis indicated that low‐amylose rice starches had lower peak viscosity, breakdown, and setback values than normal amylose rice starches. Starch granules from low‐amylose rice cultivars had a higher susceptibility to glucoamylase than those from normal amylose rice cultivars. The results of this study showed some differences between normal and low‐amylose rice starches in pasting properties and enzymatic digestibility.  相似文献