Effect of volume of non-surgical embryo transfer medium on ability of porcine embryos to survive to term     

Nakazawa Y  Misawa H  Fujino Y  Tajima S  Misumi K  Ueda J  Nakamura Y  Shibata T  Hirayama Y  Kikuchi K 《The Journal of reproduction and development》2008,54(1):30-34

Non-surgical embryo transfer is a promising method for improving efficiency in the pork industry and also for biotechnology applications, such as in vitro embryo production, transgenesis and cloning. Several groups have reported successful piglet production using an artificial insemination catheter or flexible catheter designed for this procedure; however, the efficiency of the technique is still low. The critical points that need to be addressed in order to improve this procedure are (1) the embryo deposition site and (2) volume of transfer medium associated with the embryos; however, the latter has not yet been examined systematically. In the present study, we evaluated the effect of the volume of non-surgical embryo transfer medium on the ability of porcine embryos to survive to term by using a recently produced flexible catheter. The catheter consists of a guide and an injector. Blastocysts 200-230 mum in diameter were collected from donor gilts and transferred to recipient gilts. The time required for the completion of embryo transfer using this catheter was 14.6 +/- 3.9 min. The tip of the injector was determined by laparotomy to be located in a uterine horn 20-30 cm anterior from the branching point of the uterus body. We transferred 17.0-17.3 embryos with different volumes of medium (1.6, 3.2 and 10 ml) into each of 5, 4 and 4 recipients, respectively, and pregnancy was confirmed in 4, 3 and 1 of these recipients, respectively. Three recipients in the 1.6 ml group farrowed a total of 19 piglets (4, 5 and 10 piglets, respectively). These results suggest that successful non-surgical embryo transfer is affected by the volume of transfer medium.  相似文献   

Isolation and characterization of sulfur oxidizing bacteria from cattle manure compost   总被引：1，自引：0，他引：1  

Ryoki ASANO  Takako SASAKI  Yutaka NAKAI 《Animal Science Journal》2007,78(3):330-333

Hydrogen sulfide causes offensive odors. We attempted to isolate sulfur oxidizing bacteria (SOB) from cattle manure compost. A most probable number assay could not detect SOB by using a Cha medium which had been applied in order to isolate the SOB from active sewage. Cultivation using a Cha plate medium revealed 5.75 × 10⁷ colony forming unit/g of bacteria. A single strain of SOB was isolated from a colony formed on the plates and identified as Halothiobacillus neapolitanus. This is the first report that H. neapolitanus has been found in cattle manure compost.  相似文献   

Comparison of Salmonella isolation rates in different types of egg-layer hen houses in Chiba, Japan     

Matsumoto A  Miyama M  Murakami S 《Avian diseases》2001,45(1):195-200

This study investigated the differences in Salmonella isolation rates in environmental samples taken from several types of hen houses in Chiba, Japan. In addition, for the detailed epidemiologic survey, environmental samples, hens, and rodents were collected from Salmonella-contaminated windowless houses on three farms. As a result, Salmonella was isolated from four (80%) of five farms with windowless hen houses; Salmonella enteritidis was isolated from a single windowless house. In contrast, only one serotype of Salmonella was isolated from 1 (6.7%) of 15 farms with open hen houses. In the S. enteritidis-contaminated windowless hen house, the isolation rates of S. enteritidis as compared with the other serotypes were 90.9% of environments, 94.1% of hens, and 86.4% of roof rats (Rattus rattus) that resided in the environments. In reference to the phage type (PT) of these isolates, PT1 was detected in environments and roof rats, and PT9 was detected in both these samples and in hens. Thus, the Salmonella isolation rate in hen houses seems to be associated with whether the premises are windowless or open. Moreover, roof rats appear to be the most important vectors in the spread of S. enteritidis in the windowless hen house because the S. enteritidis PTs coincide with each other.  相似文献   

A case of spontaneous Zymbal’s gland carcinoma with lung metastasis in an aged Fischer 344 rat     

Ai Maeno  Yoshimitsu Sakamoto  Motoki Hojo  Yukie Tada  Jin Suzuki  Akiko Inomata  Takako Moriyasu  Akihiko Hirose  Noriko Kemuriyama  Katsuhiro Miyajima  Dai Nakae 《Journal of toxicologic pathology》2021,34(4):353

Zymbal’s gland neoplasms are induced in rats through the administration of various carcinogens, but spontaneous neoplasia is rare. This report describes a spontaneous Zymbal’s gland carcinoma with lung metastasis found in an aged male Fischer 344 rat. Macroscopically, the dome-like tumor nodule, approximately 30 mm in diameter with ulceration, was located near the ear canal of the rat. No healthy tissue or structure of Zymbal’s gland was identified on the corresponding side, while the normal salivary glands and a lacrimal gland were observed. Histologically, a large part of the tumor mass was occupied by poorly differentiated neoplastic cells, the shapes of which were oval to polygonal or fusiform. Additionally, clusters of sebaceous-like foamy cells and squamous metaplasia with prominent keratinization were observed. Tumor cells were found to metastasize to the lung; these cells displayed histological similarities, including a sebaceous gland-like pattern, to those in the primary site. The tumor cells were immunohistochemically positive for cytokeratin AE1/AE3 or vimentin but negative for CD68, S100, α-smooth muscle actin, von Willebrand factor, and desmin. Our results indicate that the tumor was a poorly differentiated Zymbal’s gland carcinoma with lung metastasis.  相似文献   

Characterization of Murine Pituitary-Derived Cell Lines Tpit/F1, Tpit/E and TtT/GF     

Saishu YOSHIDA  Masashi HIGUCHI  Hiroki UEHARU  Naoto NISHIMURA  Mitsuyoshi TSUDA  Hideji YAKO  Mo CHEN  Hideo MITSUISHI  Yoshiya SANO  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2014,60(4):295-303

The pituitary is an important endocrine tissue of the vertebrate that produces and secretes many hormones. Accumulating data suggest that several types of cells compose the pituitary, and there is growing interest in elucidating the origin of these cell types and their roles in pituitary organogenesis. Therein, the histogenous cell line is an extremely valuable experimental tool for investigating the function of derived tissue. In this study, we compared gene expression profiles by microarray analysis and real-time PCR for murine pituitary tumor-derived non-hormone-producing cell lines TtT/GF, Tpit/F1 and Tpit/E. Several genes are characteristically expressed in each cell line: Abcg2, Nestin, Prrx1, Prrx2, CD34, Eng, Cspg4 (Ng2), S100β and nNos in TtT/GF; Cxcl12, Raldh1, Msx1 and Twist1 in Tpit/F1; and Cxadr, Sox9, Cdh1, EpCAM and Krt8 in Tpit/E. Ultimately, we came to the following conclusions: TtT/GF cells show the most differentiated state, and may have some properties of the pituitary vascular endothelial cell and/or pericyte. Tpit/F1 cells show the epithelial and mesenchymal phenotypes with stemness still in a transiting state. Tpit/E cells have a phenotype of epithelial cells and are the most immature cells in the progression of differentiation or in the initial endothelial-mesenchymal transition (EMT). Thus, these three cell lines must be useful model cell lines for investigating pituitary stem/progenitor cells as well as organogenesis.  相似文献   

Coronary artery spasm induced in atherosclerotic miniature swine   总被引：22，自引：0，他引：22  

H Shimokawa  H Tomoike  S Nabeyama  H Yamamoto  H Araki  M Nakamura  Y Ishii  K Tanaka 《Science (New York, N.Y.)》1983,221(4610):560-562

Angiographically demonstrable coronary artery spasm could be provoked repeatedly by giving intracoronary or intravenous injections of histamine to miniature swine with experimentally induced atherosclerotic lesions of the coronary artery. The spasm induced in this way subsided either spontaneously or after the administration of nitroglycerin and was prevented by a calcium antagonist or an agent that blocks histamine H1 receptors. This model, which suggests that atherosclerotic changes may be one of the primary factors in the occurrence of coronary artery spasm, should facilitate studies on the pathogenesis of this condition.  相似文献   

Mechanism of self-sterility in a hermaphroditic chordate     

Harada Y  Takagaki Y  Sunagawa M  Saito T  Yamada L  Taniguchi H  Shoguchi E  Sawada H 《Science (New York, N.Y.)》2008,320(5875):548-550

Hermaphroditic organisms avoid inbreeding by a system of self-incompatibility (SI). A primitive chordate (ascidian) Ciona intestinalis is an example of such an organism, but the molecular mechanism underlying its SI system is not known. Here, we show that the SI system is governed by two gene loci that act cooperatively. Each locus contains a tightly linked pair of polycystin 1-related receptor (s-Themis) and fibrinogen-like ligand (v-Themis) genes, the latter of which is located in the first intron of s-Themis but transcribed in the opposite direction. These genes may encode male- and female-side self-recognition molecules. The SI system of C. intestinalis has a similar framework to that of flowering plants but utilizing different molecules.  相似文献   

Sustainable meat consumption in China     

Satoru Shimokawa 《中国农业科学(英文版)》2015,(6)

Sustainable meat consumption is critical to achieve a sustainable food system because meat products are among the most energy-intensive, ecological y burdensome, and ethical y concerned foods. This pap...  相似文献   

Single particle analysis reveals that bacterial community structures are semi-specific to the type of soil particle     

Takako KOTANI-TANOI  Masaya NISHIYAMA  Shigeto OTSUKA  Keishi SENOO 《Soil Science and Plant Nutrition》2007,53(6):740-743

  相似文献   

A case of Iriomote cat (Prionailurus bengalensis iriomotensis) with Hepatozoon felis parasitemia     

Sakuma M  Nishio T  Nakanishi N  Izawa M  Asari Y  Okamura M  Shimokawa Miyama T  Setoguchi A  Endo Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1381-1384

We herein present clinical findings of an Iriomote cat with Hepatozoon felis parasitemia. A male Iriomote cat was captured for ecological analyses three times from January 2010 to January 2011. Although this cat did not show any hematological abnormalities at the time of the first capture, H. felis parasitemia and increased serum creatine kinase levels were detected at the second and third captures. H. felis infection was confirmed by polymerase chain reaction, and amplified 18S ribosomal RNA gene fragments were 100% identical to those of H. felis in leopard cats in Korea. Although the virulence of H. felis in this cat was suggested to be low, this is the first report of an H. felis-infected Iriomote cat with parasitemia.  相似文献