Virtopsy in a red kangaroo with oral osteomyelitis     

Lee KJ  Sasaki M  Miyauchi A  Kishimoto M  Shimizu J  Iwasaki T  Miyake Y  Yamada K 《Journal of zoo and wildlife medicine》2011,42(1):128-130

This report describes the use of computed tomography (CT) in a nondomestic species. Postmortem CT was performed on a red kangaroo (Macropus rufus) and a diagnosis of oral osteomyelitis was made. CT examination revealed bony remodeling of the right mandible, an intraosseous lesion of the right temporal bone, muscle necrosis around the right mandible, and the absence of the right, first, upper molar tooth. Cardiac and intrahepatic gas and a distended intestine due to postmortem gas accumulation were also seen. All the lesions identified with CT were also identified by conventional necropsy, except the cardiac and intrahepatic gases. Virtopsy may be a useful procedure for the noninvasive identification of cause of death and as a guide for necropsy in animals.  相似文献   

A broad spectrum,one-step RT-PCR to detect <Emphasis Type="Italic">Satsuma dwarf virus</Emphasis> variants using universal primers targeting both segmented RNAs 1 and 2     

Shin-ichi Shimizu  Takao Ito  Takanori Miyoshi  Yasunobu Tachibana  Tsutae Ito 《Journal of General Plant Pathology》2011,77(6):326-330

Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity.  相似文献   

Determination of tri-n-butyltin and di-n-butyltin compounds in fish by gas chromatography with flame photometric detection     

K Sasaki  T Ishizaka  T Suzuki  Y Saito 《Journal of the Association of Official Analytical Chemists》1988,71(2):360-363

An analytical method is described for the simultaneous quantitative determination of tri-n-butyltin and di-n-butyltin compounds in fish. The sample was extracted with 0.5N HCl-methanol, and the methanol solution was extracted with hexane. The extract was purified by gel permeation chromatography and treated with Grignard reagent to yield the methyl derivatives, which were determined by gas chromatography with flame photometric detection operated in the tin mode (610 nm). Recoveries of tri-n-butyltin chloride (Bu3SnCl) and di-n-butyltin dichloride (Bu2SnCl2) spiked to fish at the levels of 0.2 and 1.0 ppm ranged from 80 to 105%. Detection limits were 0.02 micrograms/g for both compounds. Tri-n-butyltin compounds equivalent to Bu3SnCl levels of 0.07-2.0 ppm and di-n-butyltin compounds equivalent to Bu2SnCl2 levels of 0.02-0.11 ppm were found in reared yellowtails, and these values showed good agreement with the results from gas chromatographic-mass spectrometric analysis.  相似文献   

Nitrogen Retention in Japanese Cedar Stands in Northern Honshu, with High Nitrogen Deposition   总被引：1，自引：0，他引：1  

M. Baba  Y. Suzuki  H. Sasaki  K. Matano  T. Sugiura  H. Kobayashi 《Water, air, and soil pollution》2001,130(1-4):1103-1108

High nitrogen, especially ammonium, input has been observed in Schichinohe, Aomori Prefecture, northeastern Japan. A monitoring study on precipitation, throughfall, and stream water has been carried out to estimate the stage of nitrogen saturation since 1996. Fifty-two to 70% of nitrogen input in throughfall was retained in forest ecosystems. Nitrate concentration in stream water tended to decrease throughout the study. There was no symptom of nitrogen saturation at Japanese cedar stands in Shichinohe, although high nitrogen input in open bulk has been observed. Ammonium (NH₄ ⁺) was retained in the canopy. The ratio of NH₄ ⁺ input in throughfall to that by open bulk was 0.40 – 0.47. Total inorganic nitrogen input under the canopy amounted 0.68 – 0.72 kmolc ha^?1 yr^?1 (9.6 – 10.0 kg N ha^?1 yr^?1). Our results suggests that atmospheric nitrogen input has benefitted the three growth.  相似文献   

Oxidative dimers produced from protocatechuic and gallic esters in the DPPH radical scavenging reaction     

Kawabata J  Okamoto Y  Kodama A  Makimoto T  Kasai T 《Journal of agricultural and food chemistry》2002,50(19):5468-5471

DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging reactions of protocatechuic and gallic acids, and their methyl esters, have been investigated by NMR. In acetone, methyl protocatechuate was gradually converted to a Diels-Alder adduct of two molecules of the intermediate quinone in the reaction with DPPH radical, whereas methyl gallate rapidly gave a symmetrical dimer via a putative quinone precursor. Both dimers are rather unstable and their structures have been deduced by in situ NMR measurements of the reaction mixtures. Gallic acid also gave a corresponding symmetrical dimer in the same reaction as methyl gallate, although protocatechuquinone produced from protocatechuic acid did not yield a Diels-Alder adduct, unlike its methyl ester. Interestingly, these dimer formations were not observed in methanol solution.  相似文献   

Effects of Rice Flour Blends on Bread Texture and Staling     

Tomoko Sasaki  Kaoru Kohyama  Kanae Miyashita  Tomoya Okunishi 《Cereal Chemistry》2014,91(2):146-151

The effects of blending rice flour with wheat flour on bread texture and staling were investigated with three rice varieties with different amylose contents. For the texture analysis of bread crumb, the compression test, puncture test, and tensile test were performed. A flour blend containing rice flour suppressed the recovery of the crumb after compression. For the puncture test, blends with rice flour increased the distance to penetrate the crumb. The rupture strain measured with the puncture test decreased with staling of the bread crumb for all samples tested. The fresh bread crumb sample containing waxy rice flour had much greater extensibility in the tensile test than the other samples tested, but it was dramatically decreased after one day of storage. Endothermic enthalpies corresponding to retrograded amylopectin, which is part of the staling process, were also measured. The enthalpy of bread crumb from the blended flour was lower than that of wheat flour bread up to three days but was higher on day 4. A blend of rice flour thus reduced amylopectin retrogradation during early storage, but it was accelerated later. Bread blended with waxy rice flour showed the lowest enthalpy during storage.  相似文献   

Results of hyperamplification of centrosomes in naturally developing tumors of dogs.     

A Setoguchi  M Okuda  E Nishida  M Yazawa  T Ishizaka  S H Hong  M Hisasue  R Nishimura  N Sasaki  Y Yoshikawa  K Masuda  K Ohno  H Tsujimoto 《American journal of veterinary research》2001,62(7):1134-1141

OBJECTIVE: To evaluate results of centrosome hyperamplification in naturally developing tumors of dogs. SAMPLE POPULATION: Tumor specimens from 9 dogs with tumors (rhabdomyosarcoma, osteosarcoma, chondrosarcoma, myxosarcoma, and mammary gland tumor) and 2 canine osteosarcoma cell lines. PROCEDURE: 3 antibodies for centrosome proteins (ie, anti-gamma-tubulin, anti-BRCA1, and anti-pericentrin) were used for immunohistochemical analysis. Double immunostaining for centrosomes was used to confirm the specificity of these antibodies for centrosomes. Mutational analysis of the canine p53 gene was carried out by polymerase chain reaction-single-strand conformation polymorphism analysis, and expression of canine MDM2 protein was evaluated by use of immunohistochemical analysis, using anti-MDM2 antibody. RESULTS: Immunohistochemical analysis of dog osteosarcoma cell lines with apparent aneuploidy revealed frequent hyperamplification of centrosomes in the osteosarcoma cell lines. Similar hyperamplified centrosomes were detected in the tumor tissues from all of the 9 tumors. The frequency of cells with hyperamplified centrosomes (3 to 20/cell) in each tumor tissue ranged from 9.50 to 48.1%, whereas centrosome hyperamplification was not observed in normal lymph nodes from these dogs. In 8 of the 9 tumors, mutation of p53 gene or overexpression of MDM2, or both, was detected. CONCLUSIONS AND CLINICAL RELEVANCE: Various types of naturally developing tumors in dogs often have hyperamplification of centrosomes associated with chromosome instability. Hyperamplification of centrosomes is a novel tumor marker for use in cytologic and histologic examinations of clinical specimens obtained from dogs.  相似文献   

Effects of genotype matching of feline major histocompatibility complex (FLA) class II DRB on skin-allograft transplantation in cats.     

Y Kuwahara  K Kito  R Kobayash  J Iwata  R Ohne  H Kitagawa  Y Sasaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1097-1101

In order to confirm the effects of matching of expressed feline major histocompatibility complex (FLA) class II DRB genotype on transplantation immunity in cats, skin-allogeneic transplantation was carried out between cats, in which DRB genes expressed were genotyped by the RT-PCR-RFLP method using group-specific primers. Duration until grafts were rejected was 14.63 +/- 1.69 days (mean +/- standard deviation) in the pairs that had the same type of subgroups, 7.25 +/- 0.71 days in the pairs that had one different type of subgroup and 6.88 +/- 0.35 days in the pairs that had two different types of subgroups. The duration of graft survival in the pairs with the same type of subgroups was significantly longer (P<0.01) than those in the pairs with different types. Although FLA components involved in transplantation immunity should not only be DRB genes, it was suggested that the expressed FLA-DRB genotype might associate with feline transplantation immunity, and that typing and matching of expressed FLA-DRB genes might be one of the important factors in the control of feline transplantation immunity.  相似文献   

The solubilization of myofibrillar proteins of vertebrate skeletal muscle in water     

Yukiko ITO  Ryuichi TATSUMI  Jun-Ichi WAKAMATSU  Takanori NISHIMURA  Akihito HATTORI 《Animal Science Journal》2003,74(5):417-425

Myofibrillar proteins of vertebrate skeletal muscles are insoluble in solutions of ionic strength that approximate physiological conditions. We established a method to solubilize more than 80% of chicken breast muscle myofibrillar proteins in water for the use of meat as a source of food protein. SDS‐polyacrylamide gel electrophoretic patterns of water‐soluble myofibrillar proteins demonstrated that all identified myofibrillar proteins except connectin/titin were soluble in water. A part of α‐actinin was released from myofibrils by repeated washing with 2.5 mmol/L NaCl and 5 mmol/L L‐histidine solution, and subsequent destruction of connectin/titin in washed myofibrils by ultrasonication resulted in solubilization of a large fraction of chicken breast muscle myofibrillar proteins in water. Myofibrillar proteins of chicken leg, pork loin, beef shoulder loin, and lamb were also solubilized in water using this procedure.  相似文献   

CDNA cloning of feline leptin and its mRNA expression in adipose tissue.     

N Sasaki  H Shibata  T Honjoh  K Kimura  M Saito  I Ohishi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(10):1115-1120

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