Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves     

Yuka KOJIMA  Shiori CHIBA  Noriyuki HORIUCHI  Yoshiyasu KOBAYASHI  Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):605-607

S100B in cerebrospinal fluid (CSF-S100B) was measured in calves with 20 neurologic and 21 non-neurologic diseases to clarify its utility as a biomarker for neurologic diseases. The median CSF-S100B value in the neurologic disease group (43.0 ng/ml) was significantly higher than that in the non-neurologic disease group (10.2 ng/ml). As CSF-S100B levels in calves with neurologic diseases widely differed, the utility of CSF-S100B as a diagnostic marker for neurologic diseases in cattle remains inconclusive.  相似文献   

Can harvest outsourcing services reduce field harvest losses of rice in China?          下载免费PDF全文

Daizo KOJIMA  Yukinaga NISHIHARA  Mitsuyoshi ANDO 《农业科学学报》2021,20(5):1396-1406

The purpose of this study is to quantify the rice harvest losses in China and to evaluate the impacts of machinery and harvest outsourcing services on these losses, given the background of high-speed mechanization and outsourcing services. Data were collected from a national survey conducted in 2016 by the research team in conjunction with the Research Center for the Rural Economy of the Ministry of Agriculture and Rural Affairs of China. A non-parametric method was used to test whether combine harvesting and outsourcing services could significantly reduce harvest losses. Next, quantile regression was used to estimate the real effects of machinery and outsourcing services on harvest losses. The analysis yielded four main study outcomes. First, the harvest loss rate of rice in China was 3.65%. Second, mechanical reaping and winnowing caused greater losses than manual methods, while the opposite was true of field transportation. Third, combine harvesting increased the losses. Fourth, the effects of an outsourcing service on losses differed among the different harvesting methods. Outsourcing services increased losses in segmented harvesting but they reduced losses in combine harvesting.  相似文献   

Spontaneous Rhabdomyosarcoma in a Common Marmoset ( Callithrix jacchus )     

Tomoaki Tochitani  Izumi Matsumoto  Kohei Hoshino  Kaoru Toyosawa  Mami Kouchi  Takatoshi Koujitani  Juki Kimura  Hitoshi Funabashi 《Journal of toxicologic pathology》2013,26(2):187-191

The common marmoset (Callithrix jacchus) is now widely used in various research fields, including toxicology. However, information about the background pathology of this species is scarce. Here, we report a case of rhabdomyosarcoma that spontaneously occurred in a common marmoset. A 44-month-old male common marmoset was euthanized due to bilateral hind limb paralysis. At necropsy, a 2×2×5-cm intramuscular mass was observed in the lower right back. Histologically, the mass was mainly composed of interlacing bundles of spindle-shaped tumor cells. Immunohistochemically, the tumor cells were positive for myogenin, desmin, vimentin and alpha-smooth muscle actin. Ultrastructurally, the tumor cells contained bundles of myofilaments with Z-band-like structures. Thus, the tumor was diagnosed as a rhabdomyosarcoma. To our knowledge, this is the first report of spontaneous rhabdomyosarcoma that was definitely diagnosed in the common marmoset.  相似文献   

Penetrating Anorectal Injury Caused by a Wild Boar Attack: A Case Report     

Ichiro Okano  Yuki Midorikawa  Nobumasa Kushima  Yui Watanabe  Takuya Sugiyama  Katsutaka Mitachi  Kazuaki Shinohara  Takatoshi Sawada  Katsunori Inagaki 《The Forestry Chronicle》2018,29(3):375-379

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Spontaneous fibrosarcoma with pleomorphic appearance in an aged brown norway rat     

Tochitani T  Toyosawa K  Matsumoto I  Kouchi M  Michimae Y  Koujitani T  Funabashi H  Seki T 《Journal of toxicologic pathology》2010,23(4):261-263

An 18-month-old male Brown Norway (BN) rat showed a grayish-white subcutaneous mass in the right cheek. Histologically, the mass was composed of highly pleomorphic cells producing collagen. Immunohistochemical analysis showed that the tumor cells were strongly positive for vimentin and partially positive for Ki-67; however, they were negative for ED-1, ED-2, S-100, cytokeratin, desmin and myoglobin. Ultrastructurally, the cytoplasms of the tumor cells contained well-developed rough endoplasmic reticulum. Thus, the tumor had no characteristic feature other than collagen production and was diagnosed as a fibrosarcoma.  相似文献   

The effect of nojirimycin on the transcriptome of germinating Orobanche minor seeds     

Atsushi Okazawa  Takatoshi Wakabayashi  Toshiya Muranaka  Yukihiro Sugimoto  Daisaku Ohta 《Journal of Pesticide Science》2020,45(4):230

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A Case Report of Feline Pituitary Carcinoma with Hypercortisolism     

Kazunori KIMITSUKI  Hassadin BOONSRIROJ  Daisuke KOJIMA  Chun-Ho PARK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):133-138

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Difference in the Use of a Quartz Filter and a PTFE Filter as First-Stage Filter in the Four-Stage Filter-Pack Method     

Masahide Aikawa  Takatoshi Hiraki 《Water, air, and soil pollution》2010,213(1-4):331-339

We evaluated the differences in the use of a quartz filter and a polytetrafluoroethylene (PTFE) filter as a first (F0)-stage filter in a four-stage filter-pack method. A four-stage filter-pack method can completely collect sulfur species (SO₂ and SO ₄ ^2? ), nitrate species (HNO₃ and NO ₃ ^? ), and ammonium species (NH₃ and NH ₄ ⁺ ) with little or no leakage irrespectively of the first-stage filter used. On the other hand, a seasonal variation was observed in the efficiency of collection between the quartz filter and the PTFE filter depending on the material to be collected. There was no seasonal variation in the efficiency of collection in sulfur species; in contrast, a clear seasonal variation was observed for the nitrate and ammonium species. As for NO ₃ ^? , the PTFE filter was more vulnerable than the quartz filter at air temperatures below 21°C, while the quartz filter was more vulnerable than the PTFE filter at air temperatures exceeding 21°C. A similar vulnerability for air temperature was observed for NH ₄ ⁺ , although the threshold air temperature was 23°C for NH ₄ ⁺ . Consequently, the evaporation loss of NO ₃ ^? would be mainly attributable to the volatilization of NH₄NO₃, although it is also partially due to the volatilization of NH₄Cl.  相似文献   

A novel method for quantification of 2-methyl-3-furanthiol and 2-furanmethanethiol in wines made from Vitis vinifera grape varieties     

Tominaga T  Dubourdieu D 《Journal of agricultural and food chemistry》2006,54(1):29-33

A rapid, easy method has been developed for isolating and quantifying 2-methyl-3-furanthiol (2M3F) in wines. Until now, it was not possible to quantify this highly odoriferous compound, with a smell reminiscent of cooked meat, in wine. The original aspect of this method is the specific release of volatile thiols using a cysteamine solution applied in reverse flow to sample percolation on the basis of a p-hydroxymercuribenzoate (pHMB)-volatile thiol conjugate formed by the direct addition of pHMB to 50 mL of wine. Purification of volatile thiols in wines is much faster and easier than our previous method. This method may also be used to assay 2-furanmethanethiol in wine. This thiol's strong aroma of roasted coffee has been shown to contribute to the "roast coffee" aroma of certain wines. Assaying 2M3F by this method showed that it was present in the wines analyzed (red and white Bordeaux, Loire Valley Sauvignon blanc, white Burgundy, and Champagne) at concentrations up to 100 ng/L, i.e., significantly above the olfactory perception threshold for this compound in model dilute alcohol solution.  相似文献   

Trisomic analysis of a strong photoperiod-sensitivity gene E1 in rice (Oryza sativa L.)   总被引：6，自引：0，他引：6  

Yutaka Okumoto  Takatoshi Tanisaka 《Euphytica》1997,95(3):301-307

Recent genetic analyses on heading-time of rice indicated that almost all the well-adapted varieties in the temperate zone carry a strong photoperiod-sensitivity gene E1, a dominant allele of E1 locus. In order to identify the chromosome on which E1 is located, a trisomic analysis was made using two primary trisomic series originating from the japonica varieties, Nipponbare and Kinmaze, respectively. The Nipponbare and Kinmaze series were crossed with heading-time tester lines, EG0 and EG3, respectively, both of which did not carry the E1. The F2 populations for chromosome 1, 2, and 3 could not be analyzed due to lack of seed. All the other F2 populations showed distinct segregation into early-type and late-type plants caused by the E1 locus segregation, which suggested that the trisomic analysis for E1 locus could be efficiently made. Both disomic and trisomic groups in the F2 population from the cross of the trisomic line for chromosome 7 × EG0 showed a segregation ratio significantly different at the 1% level from a ratio of 1 [e1e1; early]: 3 [E1e1, E1E1; late]. This suggested that E1 was located on chromosome 7. Subsequently, the linkage analysis was made using three morphological marker genes on chromosome 7. It was recognized that E1 was linked to rfs (rolled fine strip gene) and slg (slender glume gene) with recombination values of 16.3 ± 5.88 and 9.1 ± 4.72%, respectively. From these results, it can be concluded that E1 is most likely to be located on chromosome 7. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献