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21.
Kazutaka Kido Tomofumi Mochizuki Kazutoshi Matsuo Chika Tanaka Kenji Kubota Takehiro Ohki Shinya Tsuda 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(2):189-194
The single recessive gene, nsv, which confers resistance against Melon necrotic spot virus (MNSV), has recently been used to develop virus-resistant melon cultivars in Japan. However, the Chiba isolate of MNSV, a
common isolate in Japan, infected resistant cultivars when inoculated melon plants were grown at 15°C. Viral RNAs accumulated
in protoplasts from resistant cultivars at both 15 and 20°C. Mechanical inoculation of the cotyledons caused MNSV to spread
throughout the leaves at 15°C, but not at 20°C. These results support our novel hypothesis that a temperature-sensitive inactivation
of disease resistance genes occurs at the nsv locus in melon cultivars with the resistance gene grown at temperatures below 20°C.
The first and second authors contributed equally to this research. 相似文献
22.
Takehiro Ohki Takahide Sasaya Mitsuru Sayama Tetsuo Maoka 《Journal of General Plant Pathology》2017,83(4):226-230
Polymyxa graminis is a soil-borne obligate organism that transmits bymoviruses and furoviruses to barley and wheat. We analyzed rDNA-ITS sequences of P. graminis from roots of wheat and barley in fields in Japan and obtained five kinds of sequences; two sequences were almost the same as known ribotype Ia and IIa, respectively, and three were close to ribotype Ib. When infection of P. graminis was examined using PCR, ribotype Ia was detected only in barley, but ribotypes Ib and IIa were detected in both wheat and barley. Our analysis suggested that Japanese ribotype Ib transmits furoviruses and bymoviruses. 相似文献
23.
Differential Expression of Two Cytosolic Ascorbate Peroxidases and Two Superoxide Dismutase Genes in Response to Abiotic Stress in Rice 总被引：3，自引：0，他引：3
Shigeto MORITA Shinya NAKATANI Tomokazu KOSHIBA Takehiro MASUMURA Yasunari OGIHARA Kunisuke TANAKA 《水稻科学》2011,18(3):157-166
Superoxide dismutase (SOD) and ascorbate peroxidase (APX) play central roles in the pathway for scavenging reactive oxygen species in plants, thereby contributing to the tolerance against abiotic stress. Here we report the responses of cytosolic SOD (cSOD; sodCc1 and sodCc2) and cytosolic APX (cAPX; OsAPX1 and OsAPX2) genes to oxidative and abiotic stress in rice. RNA blot analyses revealed that methyl viologen treatment caused a more prominent induction of cAPXs compared with cSODs, and hydrogen peroxide t... 相似文献
24.
Aono Yoshiyuki Nakayama Takato Ozawa Takahiro Ushio Yutaka Yasuoka Shinji Fujimoto Taketo Ohki Takehiro Oka Norikuni Maoka Tetsuo 《Journal of General Plant Pathology》2021,87(4):209-218
Journal of General Plant Pathology - A BIO-PCR was developed for the detection of potato blackleg pathogens, including Pectobacterium wasabiae (Pw), P. atrosepticum (Pa), P. carotovorum subsp.... 相似文献
25.
Manabu YAMADA Kentaro MASUJIN Ken-ichiro KAMEYAMA Reiko YAMAZOE Takashi KUBO Kei IWATA Aiko TAMURA Hiroyuki HIBI Takayoshi SHIRATORI Shunjiro KOIZUMI Kousuke OHASHI Mitsutaka IKEZAWA Takehiro KOKUHO Makoto YAMAKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1835
We experimentally infected pigs with the African swine fever virus (ASFV) Armenia 07 strain (genotype II) to analyze the effect of different dose injections on clinical manifestations, virus-shedding patterns, histopathology, and transmission dynamics by direct contact. Each three pigs and four pigs were injected intramuscularly with 0.1 fifty percent hemadsorbing doses (HAD50)/ml, 101 HAD50/ml and 106 HAD50/ml of ASFV Armenia 07 strain, respectively. Each two of three pigs injected with 0.1 HAD50/ml and 101 HAD50/ml died by 10 days post inoculation. All pigs had a gross lesion of splenomegaly. Perigastric and renal lymph nodes were enlarged and resembled blood clots in nine of ten pigs. It was revealed that 0.1 HAD50/ml of this ASFV was sufficient to infect healthy pigs by intramuscular injection and caused sub-acute lethal disease. For the transmission study, two 8-week-old pigs were injected intramuscularly with 103 HAD50/ml of the same virus. Each of the experimentally inoculated pigs was co-housed with two 8-week-old naive pigs. All contact pigs exhibited clinical manifestations at 6 or 7 days after the experimentally inoculated pigs developed pyrexia. These findings suggest that this strain may spread slowly within a herd. Histologically, lymph nodes resembled blood clots were formed by severe blood absorption and followed hemorrhage result of disruption of the lymphoid sinus filling with absorbed red blood cells. The severity of the gross and histological lesions depended on duration after infection, regardless of the difference of injection doses in this study. 相似文献
26.
A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle 总被引：1，自引：0，他引：1
Hidetoshi Higuchi Hidetomo Iwano Kazuhiro Kawai Takehiro Ohta Tetsu Obayashi Kazuhiko Hirose Nobuhiko Ito Hiroshi Yokota Yutaka Tamura Hajime Nagahata 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):191-193
A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 103 cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms. 相似文献
27.
Trang VT Takeuchi H Kudo H Katsuno S Shimamura T Kashiwagi T Son VH Sugiura T Ukeda H 《Journal of agricultural and food chemistry》2011,59(16):8953-8960
In this study, antimicrobial activity of aminoreductone (AR), a product formed during the initial stage of the Maillard reaction, against methicillin-resistant Staphylococcus aureus (MRSA) was evaluated. The significant growth inhibition of all 51 MRSA isolates irrespective of drug susceptibility by AR was observed. The minimum inhibitory concentration (MIC) of AR ranged from 13 to 26 mM. The bactericidal activity of AR was evaluated by a killing assay with multiples of MIC, and it was recognized to depend on its dose. The combined effects of AR and antibiotics frequently used for the treatment of infections caused by Gram-positive and Gram-negative bacteria, such as amikacin (AN), ciprofloxacin, imipenem and levofloxacin, were examined. As a result, AR did not interfere with these antibiotic activities against 12 MRSA isolates selected and showed the advanced effect of growth inhibition in combination with antibiotics. Moreover, the inhibitory effects of AR were similar to those of AN, an antibiotic with known adverse effects, some serious. These findings show that AR is a naturally formed antimicrobial agent present in thermally processed foods with potential health benefits in medical practice. 相似文献
28.
To identify genes involved in arsenite [As(III)] tolerance, we screened for As(III)-sensitive mutants using ethyl methanesulfonate (EMS)-mutagenized seeds of Arabidopsis thaliana. One mutant with high sensitivity to As(III) was isolated from the screening. It had a mutation in Glu52 of phytochelatin synthase 1 (AtPCS1), and introduction of the wild-type AtPCS1 gene rescued the phenotype, confirming that the mutant represented a new allele of atpcs1 (termed as cad1-7). The expression of AtPCS1 from cad1-7 and cad1-3, a second allele of atpcs1, complemented As-sensitive yeast, but not plants. Our study suggests that AtPCS1 activity is differentially regulated between plants and yeast. 相似文献
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30.
Takehiro HIMAKI Taka‐aki YOKOMINE Masahiro SATO Sonshin TAKAO Kazuchika MIYOSHI Mitsutoshi YOSHIDA 《Animal Science Journal》2010,81(5):558-563
The present study was carried out to examine the effects of post‐activation treatment of trichostatin A (TSA), a histone deacetylase inhibitor, on in vitro development and transgene function of somatic cell nuclear transfer (SCNT) embryos derived from Clawn miniature pig embryonic fibroblast (PEF) transfected with a bacterial endo‐β‐galactosidase C gene (removal of the α‐galactosyl (Gal) epitope). SCNT embryos were incubated with or without TSA (50 or 100 nmol/L) after activation, cultured in vitro and assessed for cleavage, blastocyst formation and transgene function. The rate of blastocyst formation was significantly higher in SCNT embryos treated with 50 nmol/L TSA than that in control (P < 0.05), whereas the rate of cleavage and cell number of blastocyst did not differ. Following labelling with fluorescein isothiocyanate‐labelled BS‐I‐B4 isolectin, the intensity of fluorescence observed on cell‐surface was dramatically reduced in transgenic SCNT blastocyst in comparison with non‐transgenic SCNT blastocyst. However, the reduction of α‐Gal epitope expression in transgenic SCNT blastocyst was not affected by TSA treatment. The results of this study showed that post‐activation treatment with 50 nmol/L TSA is effective to improve in vitro developmental capacity of transgenic SCNT miniature pig embryos without the modification of transgene function. 相似文献