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31.
We examined the relationship between natural killer (NK) cell-mediated cytotoxicity, the produced active-oxygen and cytotoxic factor (CF) release in co-culturing canine NK cells with tumor cells (CL-1 target cells). In co-culturing, the adding of n-propyl gallate (active-oxide scavenger) removed the produced active-oxygen, which inhibited NK cell-mediated cytotoxicity and the CF release. Moreover, adding of this agent inhibited the tyrosine phosphorylation of NK intracellular protein which observed in co-culturing. Therefore, the active-oxygen produced from canine NK cells are thought to relate the signal transduction in NK-mediated cytotoxicity.  相似文献   
32.
The genome of Malassezia pachydermatis isolates from dogs was resolved into six chromosomes by using pulsed-field gel electrophoresis and their molecular sizes were calculated as 820, 1,100, 1,400, 1,470, 1,660 and 1,820 Kb, respectively. Comparison of electrophoretic patterns suggested that the chromosomes of M. pachydermatis were homozygous.  相似文献   
33.
On the productivity of paddy field, many investigations have been performed in Japan. These investigations were mainly focused on the importance of mineralization of soil organic nitrogen, redox system, and generation of injurious materials under water-logged conditions of soils. Recently HARADA (1) and YOSHIDA (2) showed that the cations adsorbed by negative charges originated from humic substances were more weaker than that of the clay. Moreover KONISHI (3) has investigated on the effects of Renge application upon the soil productivity and the leaching of nutrients. He proved that the applicatign of Renge upon soil accelerates the nutrient leaching. From these experiments the importance of nutrient leaching in the soil productivity was again closed up.  相似文献   
34.
It was observed previously that, when a fully developed leaf of the rice plant (Oryza sativa) was detached and kept in the light, more rapid loss of chlorophyll took place than in the dark during their incubation. Since this had been considered to be associated with photosynthesis, the effects of CMU (p-chlorophenyl dimethyl urea) and other compounds on the degradation of chlorophyll were studied. CMU had a significant effect in retarding the degradation of chlorophyll in the light irrespective of the presence of sucrose, whereas monoiodo acetic acid was far less effective. The degradation of chlorophyll was greatly promoted, especially in the light, when a detached leaf was kept in oxygen gas, but was delayed in nitrogen gas.

Electron microscopic observation showed that grana fret-work system and chlorophyll membrane were maintained in a healthy condition for the first few days during treatment with CMU. But they were completely degraded without CMU, and the disorganization of chloroplasts was accompanied by a rapid degradation of chlorophyll. It was assumed that oxygen evolved in photosynthetic reaction causes light-induced degradation of chlorophyll, and CMU represses degradation by inhibiting oxygen evolution in the light reaction of photosynthesis. A suggestion was made as to the scheme of light-induced degradation of chlorophyll relative to photosynthetic reaction and the mode of action of CMU.  相似文献   
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