Replication of the B19 parvovirus in human bone marrow cell cultures   总被引：30，自引：0，他引：30  

K Ozawa  G Kurtzman  N Young 《Science (New York, N.Y.)》1986,233(4766):883-886

The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients with hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.  相似文献   

Development of plaque techniques for titration and neutralization tests with African horse-sickness virus   总被引：1，自引：0，他引：1  

I G Hopkins  A Hazrati  Y Ozawa 《American journal of veterinary research》1966,27(116):96-105

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Goat meat acceptance in Japan: Current situation and future prospects     

Takeyuki OZAWA  Jiro NISHITANI  Sachiko ODAKE  Nicolas LOPEZ-VILLALOBOS  Hugh T. BLAIR 《Animal Science Journal》2005,76(4):305-312

Three surveys and a sensory evaluation of goat meat were conducted in Japan to examine the current needs and future possibilities of goat meat consumption. The experimental results of the present study lead us to conclude the following: (i) improving the smell of goat meat and combining the sale of goat meat with recipes may increase goat meat demand among housewives; (ii) it seems to be difficult to disseminate Okinawa goat cuisine to non‐Okinawa residents because of the typical odor of goat meat, which is unlikely to be preferred by the public; (iii) the “chewiness” of goat meat is rated highly by young participants according to a sensory evaluation, and loin meat is rated highly compared with hind leg meat; and (iv) there is a rapidly increasing interest and a tremendous purchasing drive towards goat meat among university students. However, these facts call for further investigation of the goat meat demand in Japan. The following three further experiments are needed to solve this issue: (i) breeding to improve the taste of goat meat; (ii) expanding the marketing of goat meat combined with the invention of Japanese‐style goat recipes and tie‐in sales supermarkets; and (iii) implementing further sensory analyses to compare goat meat with other meat and carrying out market analysis in Japan.  相似文献   

Plasma metabolite concentrations and hepatic enzyme activities in pregnant Romney ewes with restricted feeding     

Tanaka Y  Mori A  Tazaki H  Imai S  Shiina J  Kusaba A  Ozawa T  Yoshida T  Kimura N  Hayashi T  Kenyon PR  Blair H  Arai T 《Research in veterinary science》2008,85(1):17-21

Plasma metabolite concentrations and activities of enzymes related to energy metabolism in plasma, peripheral leukocytes and liver of pregnant Romney ewes with restricted feeding were measured to assess those metabolites and enzymes as indicators for evaluating metabolic conditions in the ewes. The body weights and plasma lactate concentrations of the low-feeding ewes (about 1.0 times maintenance) were significantly lower than those of the high-feeding ewes (about 1.5 times maintenance). There were no significant differences in plasma protein, glucose, triglyceride, cholesterol, pyruvate and immunoreactive insulin concentrations and plasma and leukocyte enzyme activities between both groups. Hepatic malate dehydrogenase activities were significantly higher and hexokinase activities were significantly lower in the low-feeding ewes than in the high-feeding ewes. Restricted feeding could maintain pregnancy and some plasma metabolites and peripheral leukocyte enzymes may be useful indicators for evaluating metabolic changes in ewes.  相似文献   

Changes in metabolite, energy metabolism related enzyme activities and peripheral blood mononuclear cell (PBMC) Populations in beef heifers with two differing liveweight change profiles in New Zealand     

Mori A  Kenyon PR  Mori N  Yamamoto I  Tanaka Y  Suzuki N  Tazaki H  Ozawa T  Hayashi T  Hickson RE  Morris ST  Blair H  Arai T 《Veterinary research communications》2008,32(2):159-166

Metabolite and immunoreactive insulin (IRI) concentrations, energy metabolism related enzymes activities and peripheral blood mononuclear cell (PBMC) populations were measured in blood of pregnant Angus heifers with differing liveweight change profiles (gaining or losing), in New Zealand to investigate the meanings of those parameters in the restricted feeding beef heifers. Beef heifers losing liveweight (−412 g/day) showed significantly lower concentrations of plasma IRI, and higher concentrations of plasma free fatty acid (FFA) than heifers gaining liveweight (483 g/day). The cytosolic and mitochondrial malate dehydrogenase (MDH) activities and MDH/lactate dehydrogenase (M/L) ratio in leukocytes of the liveweight losing heifers were significantly higher than those the liveweight gaining heifers. Percentages of cluster of differentiation (CD) 3 positive cells and natural killer (NK) cells in PBMC decreased significantly in the liveweight losing heifers compared to those in the liveweight gaining heifers. Plasma IRI and FFA concentrations, leukocyte cytosolic and mitochondrial MDH activities and CD3 positive and NK cell populations may be useful markers to evaluate metabolic conditions and immunity in the restricted feeding beef heifers.  相似文献   

Changes of multi-drug resistance pattern in Salmonella enterica subspecies enterica serovar typhimurium isolates from food-producing animals in Japan     

Kawagoe K  Mine H  Asai T  Kojima A  Ishihara K  Harada K  Ozawa M  Izumiya H  Terajima J  Watanabe H  Honda E  Takahashi T  Sameshima T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1211-1213

A total of 153 isolates of Salmonella Typhimurium derived from food-producing animals in Japan between 2002 and 2005 were investigated for antimicrobial resistance and phage types related to definitive phage type 104 (DT104). The predominant resistance type was resistance to ampicillin, dihydrostreptomycin, kanamycin, and oxytetracycline in bovine (45.2%, 48/104) and resistance to dihydrostreptomycin and oxytetracycline in porcine isolates (58.7%, 27/48). DT104-related phage type was found in 32 of 104 bovine isolates, two of 48 porcine isolates, and one of eight isolates from poultry, showing that the proportion of the phage type in S. Typhimurium isolates from cattle and pigs significantly (P<0.01) decreased from 71.9% and 31.4% in 1999-2001 to 30.8% and 4.1% in 2002-2005, respectively.  相似文献   

Isolation and identification of F-spondin in the boar testis and its production during testis growth     

Ohnuma K  Kaneko H  Noguchi J  Kikuchi K  Ozawa M  Hasegawa Y 《The Journal of reproduction and development》2007,53(1):151-158

F-spondin/vascular smooth muscle cell growth-promoting factor (VSGP), purified from the follicular fluid of adult bovine ovaries, has been identified as a promoter of neuronal differentiation and vascular smooth muscle growth. The objectives of the present study were (1) to clarify whether F-spondin is also produced in the testis, which is ontogenically equivalent to the ovary, and (2) to examine whether production of this protein changes with testicular growth. To isolate F-spondin from the testis, testicular homogenates obtained from 8-week-old boars were sequentially subjected to heparin-Sepharose chromatography, diethylaminoethyl (DEAE)-Sepharose chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The isolated protein had a molecular mass of approximately 110 kDa and was cross-reactive with anti-F-spondin antibody by Western blotting. The purified protein was further characterized by amino acid sequence analysis of its internal peptide. The sequence obtained was GEQCNIVPDN VD, and a homology search indicated that the purified protein is a homologue of rat, human, and bovine F-spondin. By fractionation of the same amounts of testis tissue obtained from 1-, 8-, 16-, and 40-week-old boars, we analyzed age-related production of F-spondin in the testis. Western blotting of the fractions obtained from RP-HPLC revealed the presence of a band at approximately 110 kDa, corresponding to F-spondin, in the testes obtained from boars between 1 and 16 weeks old, but this band was not detected at 40 weeks. These results clearly indicate that (1) the porcine testis produces F-spondin and that (2) production of this protein is evident in the immature porcine testis, but not the adult testis.  相似文献   

Comparison of plasma metabolite concentrations and enzyme activities in beef cattle raised by different feeding systems in Korea, Japan and New Zealand   总被引：2，自引：0，他引：2  

Mori A  Urabe S  Asada M  Tanaka Y  Tazaki H  Yamamoto I  Kimura N  Ozawa T  Morris ST  Hickson R  Kenyon PR  Blair H  Choi CB  Arai T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(7):342-345

Concentrations of metabolites and immunoreactive insulin (IRI) and activities of enzymes related to energy metabolism were measured in plasma of Korean and Japanese beef cattle, which were raised by the indoor feeding system programmed to feed larger amount of roughage in their growing periods and larger amount of concentrate diet in their finishing periods (Japanese feeding system), and grazing New Zealand beef cattle. By the Japanese beef grading system, Korean and Japanese beef cattle showed high beef quality score, average grade 3.3 and 3.6, respectively. The plasma free fatty acid and lactate concentrations and lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and aspartate aminotransferase (AST) activities in Korean beef cattle were significantly higher than those in Japanese beef cattle. The plasma lactate concentration in Korean beef cattle was 8.40 mmol/l, which was similar to the values observed in lactic acidosis. The higher activities of plasma LDH, MDH and AST may indicate slight liver damage by slightly acidotic conditions in Korean beef cattle. New Zealand beef cattle fed on pasture which they harvest by grazing showed significantly lower plasma glucose, cholesterol, lactate and IRI concentrations and enzyme activities than those in Korean and Japanese beef cattle fed on larger amount of concentrate diets. Plasma metabolite concentrations and energy metabolism-related enzyme activities may be good indicators for evaluating metabolic conditions of beef cattle raised by different feeding systems.  相似文献   

Outcomes of dogs with progressive myelomalacia treated with hemilaminectomy or with extensive hemilaminectomy and durotomy     

Yuya Nakamoto  Takashi Uemura  Hiroki Hasegawa  Miwa Nakamoto  Tsuyoshi Ozawa 《Veterinary surgery : VS》2021,50(1):81-88

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Formation of a one-dimensional array of oxygen in a microporous metal-organic solid     

Kitaura R  Kitagawa S  Kubota Y  Kobayashi TC  Kindo K  Mita Y  Matsuo A  Kobayashi M  Chang HC  Ozawa TC  Suzuki M  Sakata M  Takata M 《Science (New York, N.Y.)》2002,298(5602):2358-2361

We report the direct observation of dioxygen molecules physisorbed in the nanochannels of a microporous copper coordination polymer by the MEM (maximum entropy method)/Rietveld method, using in situ high-resolution synchrotron x-ray powder diffraction measurements. The obtained MEM electron density revealed that van der Waals dimers of physisorbed O2 locate in the middle of nanochannels and form a one-dimensional ladder structure aligned to the host channel structure. The observed O-O stretching Raman band and magnetic susceptibilities are characteristic of the confined O2 molecules in one-dimensional nanochannels of CPL-1 (coordination polymer 1 with pillared layer structure).  相似文献