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311.
Mikimasa Joh Taihei Matsuda Nobukazu Satoh Nobuyuki Tanaka Yoshiyuki Ueda 《Fisheries Science》2011,77(5):773-783
Brown sole Pseudopleuronectes herzensteini larvae and juveniles were reared to validate daily otolith ring formation. At 15°C, a check (a distinct ring) formed on the
sagittae and lapilli at 6 days after hatching, and clear increments regularly formed outside the check. For both otoliths,
the relationship between the number of days after hatching and number of increments was linear, and the slope of the line
was approximately 1; therefore, daily formation was validated. At 12°C, the check formed on the lapillus 8 days after hatching.
Accessory primordia (AP) began forming on the sagittae of metamorphosing larvae, and the shape of the sagittae became complicated.
AP were not formed on the lapillus; concentric rings were formed throughout larval and juvenile stages. Wide and obscure increments
formed on the lapilli during metamorphosis (metamorphosing zone, MZ). Based on MZ, concentric rings that have formed on the
lapilli of juveniles can be separated into larval and juvenile rings. The morphs of large juveniles’ lapilli were bilaterally
asymmetric, and the blind-side lapilli were most suitable for otolith microstructure analysis. This study provides fundamental
information for otolith microstructure analysis in wild brown sole. 相似文献
312.
Takumi Fujino Hideki Sawada Hiromichi Mitamura Reiji Masuda Nobuaki Arai Yoh Yamashita 《Fisheries Science》2017,83(3):367-372
Populations of sea cucumbers, including the Japanese common sea cucumber Apostichopus japonicus, have been seriously depleted worldwide due to overfishing. Mark–recapture study is an efficient means of collecting ecological data. However, the use of such a method in sea cucumbers is difficult because they lack hard tissues in the body wall. Here we tested the viability of various tagging methods on A. japonicus. First, we applied conventional tags using four different methods [single spaghetti (T-bar) tagging, double spaghetti tagging, ribbon tagging, and Atkins tagging] to ten individuals per method in aquaria for 14 days. Of the methods used, single spaghetti tagging had the highest retention rate. Then we examined the retention rate of single spaghetti tags on ten individual sea cucumbers for up to approximately 6 months in rearing conditions. The single spaghetti tagging method showed a retention rate of 100% over at least 7 days, and 50% of the tags remained embedded after 56 days. The longest duration of tag retention was 174 days, at which time the experiment was terminated. These results indicate that single spaghetti tagging is reliable for both short- and longer-term studies, making it a useful tool for ecological and conservation studies in sea cucumbers. 相似文献
313.