Effects of peripheral blood polymorphonuclear leukocyte function and blood components in Japanese black steers administered ACTH in a cold environment.     

H Ishizaki  Y Kariya 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(5):487-492

An examination of the effects of artificial stress induced by adrenocorticotropin (ACTH) on total and differential leukocyte counts, plasma cortisol levels, metabolic profiles and peripheral blood polymorphonuclear leukocyte (PMN) function was performed on Japanese Black steers kept in a cold environment, with the following regimes; 1) -5 degrees C x ACTH (100 IU/day for 3 days), 2) 0 degrees C x ACTH, 3) 15 degrees C x ACTH and 4) 15 degrees C x PBS. Blood samples were collected before and at 1, 2, 24, 48, 72 and 96 hr prior to the application of the stressor. The plasma cortisol level was found to greatly increase at one hr after the first treatment of ACTH, particularly so in animals exposed to -5 degrees C. Total leukocytes (-5 degrees C and 0 degrees C experiments, respectively), the monocytes (-5 degrees C), neutrophils and eosinophils (-5 degrees C, 0 degrees C and 15 degrees C, respectively) obviously increased just after the first administration, although lymphocyte counts at -5 degrees C were inversely related to those described above. All of these tendencies were augmented by the cold environment except for eosinophils. The chemiluminescent (CL) response of PMN decreased in the ACTH-administered steers at an early stage of post-administration, however, it tended to recover from the lower-than-base value in the cold-affected steers. ACTH administration resulted in higher plasma glucose (Glu) compared to a control, although only steers housed at -5 degrees C evidently showed lower plasma inorganic phosphorus (IP). No abnormal serum acute phase protein, or immunosuppressor, was noted. ACTH thus appears not only to promote physiological reactions but also to temporarily suppress PMN cellular immune function in Japanese Black steers. Although, a cold environment rapidly restored the CL activity to over the pre-administrational value, suggesting that a vital response was activated by crymo-stimuli.  相似文献   

Evaluation of the section modulus for tree-stem cross sections of irregular shape     

Akio Koizumi  Takuro Hirai 《Journal of Wood Science》2006,52(3):213-219

The effect of section modulus, as estimated for tree stems of irregular cross section with hollow trunks, on windthrow resistance is discussed. The sample trees were 12 aged poplar trees growing along the roadside in Sapporo. Binarized bitmap images of photographs of the crosscut surface of the sample tree stems were used to calculate numerical solutions for section moduli. The error for image resolution was simulated to less than 1% under the condition in which the image was divided into more than 400 pixels. The coefficient of variation in section modulus concerning six neutral axis directions in a horizontal plane was 11%–14%. The reduction in section modulus caused by decay or hollow trunk was 36%–56% of the full cross section. The effect of section modulus on critical wind velocity was found to be considerable. The critical wind velocity calculated for some of the sample trees was less than 40m/s, and these trees were considered to be in danger of bending failure of the stems.  相似文献   

Anti-CD71 antibody immunohistochemistry in the diagnosis of acute myeloid leukemia,subtype acute erythroid leukemia with erythroid dominance (AML M6-Er), in a retrovirus-negative cat     

Satoshi Suzuki  Naotaka Ogino  Ikki Mitsui  Hiroyuki Ito  Takuro Kariya 《Journal of veterinary diagnostic investigation》2021,33(1):87

CD71 is an immunohistochemical marker used in diagnosing acute myeloid leukemia (AML) M6-Er in humans; however, to our knowledge, it has not been reportedly used for immunohistochemistry in veterinary medicine. We evaluated the pathologic features of AML M6-Er in a retrovirus-negative cat and used CD71 to support the diagnosis. A 4-y-old spayed female Scottish Fold cat was presented with lethargy, anorexia, and fever. Whole-blood PCR assay results for pro feline leukemia virus/pro feline immunodeficiency virus and feline vector-borne diseases were negative. Early erythroid precursors were observed in the peripheral blood smear. Fine-needle aspiration of the enlarged spleen and splenic lymph node showed many early erythroid precursors. Bone marrow aspirate smears revealed erythroid hyperplasia with 68.4% erythroid lineage and 3.6% rubriblasts. Dysplastic cells infiltrated other organs. The patient was diagnosed with myelodysplastic syndrome, progressing to the early phase of AML M6-Er. The patient died on day 121 despite multidrug treatments. Postmortem examination revealed neoplastic erythroblasts infiltrating the bone marrow and other organs. Neoplastic cells were immunopositive for CD71 but immunonegative for CD3, CD20, granzyme B, von Willebrand factor, CD61, myeloperoxidase, and Iba-1. Although further studies are necessary for the application of CD71, our results supported the morphologic diagnosis of AML M6-Er.  相似文献   

Effect of panicle removal on cytokinin level in the xylem and nitrogen uptake activity of rice     

Takuro SHINANO  Motonari OSAWA  Hiroshi SOEJIMA  MITSURU OSAKI 《Soil Science and Plant Nutrition》2006,52(3):331-340

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Globule leukocyte neoplasm in a cat     

K Kariya  A Konno  T Ishida  T Washizu  I Tomoda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):403-405

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Seroepidemiological survey of morbillivirus infection in Kuril harbor seals (Phoca vitulina stejnegeri) of Hokkaido, Japan     

Fujii K  Sato H  Kakumoto C  Kobayashi M  Saito S  Kariya T  Watanabe Y  Sakoda Y  Kai C  Kida H  Suzuki M 《The Japanese journal of veterinary research》2006,54(2-3):109-117

Serological analysis was performed to detect morbillivirus infection in Kuril harbor seals in Hokkaido, Japan. Serum samples were collected from the seals at Nosappu (231 sera), Akkeshi (16), and Erimo (75) between 1998 and 2005. Antibodies to phocine distemper virus (PDV) were detected by ELISA in seals from Nosappu and Erimo. Antibodies to PDV were found in 56% (5/9) of the sampled seals from Nosappu in 1998, versus only 5% (3/66) for 2003, 1% (1/79) for 2004, and 1% (1/77) for 2005. These suggest epidemic caused by the virus in or before 1998. As antibody-positive seals included juvenile seals in 2003 and 2005, sporadic infections of the virus are thought to have occurred in recent years. In Erimo, antibodies to PDV were found in 50% (14/28) of sampled seals in 2004, versus only 13% (1/8) for 1999, 7% (1/15) for 2003, and 0% (0/24) for 2005. These suggest sporadic infection by the virus before 2003 and the epizootic between after autumn in 2003, when samples of 2003 were collected, and 2004. Since antibodies to canine distemper virus (CDV) were detected in one adult seal from Nosappu in each year from 2003 to 2005, sporadic infections of the virus were suggested. There were no difference in incidence of seals with antibodies to the viruses between males and females and between juveniles and adults.  相似文献   

A syngeneic hepatocellular carcinoma model rapidly and simply prepared using a hydrodynamics-based procedure     

Kang JH  Mori T  Niidome T  Katayama Y 《Veterinary journal (London, England : 1997)》2009,181(3):336-339

A rapid and simple method to deliver tumor cells into the liver by intravenous injection of a large volume of tumor cell solution at a high velocity has been developed for use in the preparation of hepatic cancer mouse models. With this hydrodynamics-based procedure, there was a 100% tumor occurrence in the liver and lungs of mice at 2 and 3 weeks, respectively. In contrast, mice injected using a non-hydrodynamics-based system showed no tumor occurrence in the liver 3 weeks after injection, although tumors were present in the lungs. The technique may be useful in the rapid development of hepatic cancer mouse models and in reducing animal mortality during model preparation.  相似文献   

Analysis of bacterial communities on alkaline phosphatase genes in soil supplied with organic matter   总被引：2，自引：0，他引：2  

Michihiko SAKURAI  Jun WASAKI  Yuiko TOMIZAWA  Takuro SHINANO  Mitsuru OSAKI 《Soil Science and Plant Nutrition》2008,54(1):62-71

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Genetic variability of maternal effect on body measurements and its intra‐ and inter‐genetic relationship with direct effect in Japanese Black calves     

Tahmina MUNIM  Takuro OIKAWA  Takayuki IBI  Tetsuo KUNIEDA  Md. Azharul HOQUE 《Animal Science Journal》2012,83(3):199-206

This study was conducted to evaluate the importance of maternal effect on body measurement traits at an early stage of growth, and to estimate the genetic relationships between direct and maternal effects and among body measurement traits at 0 month (0‐mo) and 4 months (4‐mo) of age in a population of Japanese Black calves. Body measurements and body weight of 889 Japanese Black calves were estimated with the use of an animal model by the Residual Maximum Likelihood procedure. Direct heritabilities were low to moderate, ranging between 0.17 ± 0.09 and 0.48 ± 0.13 at 0‐mo, and slightly lower, ranging between 0.15 ± 0.07 and 0.33 ± 0.13 at 4‐mo. Estimated maternal heritabilities were low to moderate, ranging between 0.08 ± 0.07 and 0.33 ± 0.07 at 0‐mo and 0.13 ± 0.06 to 0.33 ± 0.06 at 4‐mo. The direct genetic correlations between 0‐mo and 4‐mo were moderate to highly positive, ranging from 0.53 ± 0.23 to 0.96 ± 0.09. The estimated direct genetic correlation of chest width with other width traits was low and positive at both ages, whereas with hip width it was high and positive (0.80 ± 0.09) at 0‐mo, suggesting that simultaneous improvement of body width of the front and back parts is possible. Maternal genetic effects were relatively independent of direct genetic effects for body measurement traits and can be considered in genetic evaluation.  相似文献   

Effective lateral resistance of timber–plywood–timber joints connected with nails     

Okumu Gordon Wanyama  Kei Sawata  Takuro Hirai  Akio Koizumi  Yoshihisa Sasaki 《Journal of Wood Science》2012,58(4):315-321

In this study, an experimental study was conducted on the nailed timber–plywood–timber joints extended from the standard wall–floor joints of wooden light frame constructions, where the bottom plates of shear walls are nailed to the floors consisting of joists and floor sheathings nailed to them. The principal conclusions are as follows: The allowable lateral resistance of the nailed timber–plywood–timber joints can roundly be estimated by neglecting the plywood panels if their densities are higher than those of the timber main-members and they are fastened effectively onto the timber main-members. The stiffness of the timber–plywood–timber joints is less than that of the control timber–timber joints, which is improved by increasing the number of nails used to fasten the plywood panels onto the timber main-members. The stiffness of the joints whose floor sheathings are glued onto the joists is equivalent to the control timber–timber joints. The timber–plywood–timber joints with appropriate specifications have greater energy capacity until the failure than that of the control timber–timber joints. This ensures their energy capacity, which is important in dynamic resistance, to be equivalent to the control timber–timber joints.  相似文献