Ecological evaluation of polymetallic soil quality: the applicability of culture-dependent methods of bacterial communities studying     

Gorovtsov  Andrey  Minkina  Tatiana M.  Morin  Tatiana  Zamulina  Inna V.  Mandzhieva  Saglara S.  Sushkova  Svetlana N.  Rajput  Vishnu 《Journal of Soils and Sediments》2019,19(8):3127-3138

Purpose

This work aimed to study the effect of long-term polymetallic contamination on the state and parameters of soil bacterial communities, including the abundance of different groups of culturable bacteria and the activity of nitrification.

Materials and methods

Monitoring plots were located in the dry lake and surrounding area, which had been formerly used for the discharge of industrial waste. The soils in the 16 plots were characterized by extremely high levels of heavy metal pollution. This study evaluated the main soil physicochemical properties by various methods, total metal contents by X-ray analysis, mobile metal content by atomic absorption spectrophotometry, the abundance of chosen groups of culturable bacteria by inoculation on solid media, and nitrification activity from ammonium and nitrite oxidation rates.

Results and discussion

High adaptation capacity of microbial communities to long-term pollution was revealed through marked lack of decrease in the abundance of some of the bacterial groups in soils with high contamination levels. Among the bacteria determined by the colony count method, copiotrophic and spore-forming bacteria were the least sensitive to contamination, and actinomycetes were the most sensitive. The high levels of soil pollution with heavy metals had pronounced adverse effects on nitrification activity. The decrease in activity was strongly correlated with pollutant concentrations. The oxidation of nitrite was shown to be more affected by pollution that the oxidation of ammonium.

Conclusions

Some groups and parameters of culturable microorganisms can be used for soil status estimation under pollution conditions even though they are only a small fraction of the microbial community. The most sensitive parameter was the nitrification rate, while the number of actinomycetes was found to be most promising parameter among the groups of bacteria determined by plate counts. The use of sensitive groups of culturable microorganisms for bioindication purposes is a method, which may provide a cheap and sufficiently reliable tool for large-scale soil monitoring studies.

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Comparison of temperature readings from a percutaneous thermal sensing microchip with temperature readings from a digital rectal thermometer in equids     

Robinson TR  Hussey SB  Hill AE  Heckendorf CC  Stricklin JB  Traub-Dargatz JL 《Journal of the American Veterinary Medical Association》2008,233(4):613-617

OBJECTIVE: To compare temperature readings from an implantable percutaneous thermal sensing microchip with temperature readings from a digital rectal thermometer, to identify factors that affect microchip readings, and to estimate the sensitivity and specificity of the microchip for fever detection. DESIGN: Prospective study. ANIMALS: 52 Welsh pony foals that were 6 to 10 months old and 30 Quarter Horses that were 2 years old. PROCEDURES: Data were collected in summer, winter, and fall in groups 1 (n = 23 ponies), 2 (29 ponies), and 3 (30 Quarter Horses), respectively. Temperature readings from a digital rectal thermometer and a percutaneous thermal sensing microchip as well as ambient temperature were recorded daily for 17, 23, and 19 days in groups 1 through 3, respectively. Effects of ambient temperature and rectal temperature on thermal sensor readings were estimated. Sensitivity and specificity of the thermal sensor for detection of fever (rectal temperature, >or= 38.9 degrees C [102 degrees F]) were estimated separately for data collection at ambient temperatures 15.6 degrees C. RESULTS: Mean ambient temperatures were 29.0 degrees C (84.2 degrees F), -2.7 degrees C (27.1 degrees F), and 10.4 degrees C (50.8 degrees F) for groups 1 through 3, respectively. Thermal sensor readings varied with ambient temperature and rectal temperature. Rectal temperatures ranged from 36.2 degrees to 41.7 degrees C (97.2 degrees to 107 degrees F), whereas thermal sensor temperature readings ranged from 23.9 degrees (75 degrees F) to 42.2 degrees C (75 degrees to 108 degrees F). Sensitivity for fever detection was 87.4%, 53.3%, and 58.3% in groups 1 to 3, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: The thermal sensor appeared to have potential use for initial screening of body temperature in equids at ambient temperatures > 15.6 degrees C.  相似文献   

Relevance of using a human microarray to study gene expression in heaves-affected horses   总被引：1，自引：0，他引：1  

Ramery E  Closset R  Bureau F  Art T  Lekeux P 《Veterinary journal (London, England : 1997)》2008,177(2):216-221

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Symmetric dimethylarginine concentrations in dogs with International Renal Interest Society stage 4 chronic kidney disease undergoing intermittent hemodialysis     

Andr Nanny Vieira Le Sueur  Silvano Salgueiro Geraldes  Alessandra Melchert  Regina Kiomi Takahira  Michael Coyne  Rachel Murphy  Donald Szlosek  Priscylla Tatiana Chalfun Guimares‐Okamoto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2635-2643

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A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans     

Andrei V. Grinchenko  Alex von Kriegsheim  Nikita A. Shved  Anna E. Egorova  Diana V. Ilyaskina  Tatiana D. Karp  Nikolay V. Goncharov  Irina Y. Petrova  Vadim V. Kumeiko 《Marine drugs》2021,19(12)

C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin-like manner. A new protein MkC1qDC from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific was purified. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC sequence was assembled using de novo mass-spectrometry peptide sequencing complemented with N-terminal Edman’s degradation, and included 176 amino acid residues with molecular mass of 19 kDa displaying high homology to bivalve C1qDC proteins. MkC1qDC demonstrated antibacterial properties against Gram-negative and Gram-positive strains. MkC1qDC binds to a number of saccharides in Ca²⁺-dependent manner which characterized by structural meta-similarity in acidic group enrichment of galactose and mannose derivatives incorporated in diversified molecular species of glycans. Alginate, κ-carrageenan, fucoidan, and pectin were found to be highly effective inhibitors of MkC1qDC activity. Yeast mannan, lipopolysaccharide (LPS), peptidoglycan (PGN) and mucin showed an inhibitory effect at concentrations three orders of magnitude greater than for the most effective saccharides. MkC1qDC localized to the mussel hemal system and interstitial compartment. Intriguingly, MkC1qDC was found to suppress proliferation of human adenocarcinoma HeLa cells in a dose-dependent manner, indicating to the biomedical potential of MkC1qDC protein.  相似文献   

Marine and freshwater crab meals in diets for red porgy (Pagrus pagrus): effect on growth,fish composition and skin colour     

Josefa Romero García  Carmen Tatiana H. Kalinowski  María Soledad L. Izquierdo  Lidia Esther R. Robaina 《Aquaculture Research》2010,41(12):1759-1769

River crab (RC) meal (Procambarus clarkii) and marine crab (MC) meal (Chaceon affinis) were tested as a partial replacement for fish meal in diets for red porgy (Pagrus pagrus), and their effects on growth performance, fish proximate composition and skin colouration were evaluated. Red porgy were fed during 165 days with five diets. High‐quality fish meal diet was used as a control diet (CD). Protein of fish meal in the control was replaced by increasing the dietary levels of protein derived from RC and MC by up to 10% and 20% of each of them (RC10, RC20, MC10 and MC20). Fish fed on MC20 showed the highest values in feed intake, weight gain and growth (%). No differences were found in FCR and protein efficiency ratio among the treatments. Inclusion of both crab meals in diets significantly decreased the lipid content in whole fish compared with the control animals. On the other hand, no differences in muscle composition were found between the diets. Feeding both crab meals resulted in colour improvement compared with that of the control fish, with better hue values for the RC meal group than those for the MC meal group. The crab meals tested in the present study are suitable as a partial replacement for fish meal in diets for the red porgy, with the MC meal improving growth and both crabs meals improving skin colour, with further improvements in skin colour produced in fish‐fed diets containing the RC meal.  相似文献   

Spatial segregation of striped marlin (Kajikia audax) by size in the eastern Pacific Ocean     

Tatiana A. Acosta‐Pachn  Mariana L. Muzquiz‐Villalobos  Sofia Ortega‐García  Raúl O. Martínez‐Rincn 《Fisheries Oceanography》2019,28(2):203-211

Striped marlin (Kajikia audax) is an epipelagic fish distributed in oceanic and coastal waters of the Pacific Ocean. This species is usually found in warm and coastal waters with high primary productivity. The main goal of this study was to describe the spatial segregation of striped marlin by average Eye‐Fork length (EFL) in the eastern Pacific Ocean (EPO) and its relationship with environmental variables using EFL data obtained from tuna purse‐seining and Generalized Additive Models (GAMs). The model suggested that larger individuals of striped marlin were more likely to be found in waters with high Chlorophyll‐a concentration (>2 mg/m³) and with temperatures lower than 25°C, within a region known as the “cold tongue” and the Humboldt current system, while smaller individuals were more likely to be found in warmer and low productive areas within a region known as the “warm pool of the EPO.” We observed that set type caused a large variation on average EFL of striped marlin; larger fish were captured in sets associated with floating objects (natural and manmade), while smaller fish were captured in sets associated with dolphins. Despite this, our findings suggest that striped marlin has a latitudinal gradient in average EFL; larger individuals occurred predominantly south of 10°N, while smaller ones occurred predominantly in coastal waters between 10°N and 20°N, thus demonstrating a spatial segregation of the species affected by its maturity stage.  相似文献   

Intra‐cohort cannibalism in early life stages of pikeperch     

Tatiana Colchen  Pascal Fontaine  Yannick Ledor  Fabrice Teletchea  Alain Pasquet 《Aquaculture Research》2019,50(3):915-924

Cannibalism has been described in 390 teleost fish species belonging to 104 families. In rearing conditions, intra‐cohort cannibalism is one of the major bottlenecks during the early life stages of pikeperch (Sander lucioperca). This study aims to describe precisely the occurrence and the onset of cannibalism of this species during the first two post‐hatching months. In a first step, the cannibalistic behaviour was analysed through observations in three experiments. In each experiment, the number of cannibalistic cases was similar. From 14 to 48 days post‐hatching (dph), more than 60 % of prey ingestions were realized by tail‐first, but after 48 dph, the number of prey ingestions by head‐first increased. In a second step, the behavioural sequence exhibited to attack and to capture a conspecific was analysed, taking into account the size ratio between the cannibal and its prey. The behavioural sequence to capture was always the same, similar to a predatory sequence and it seems that the cannibal chose its prey about 10 s before attacking. Moreover, prey choice by a cannibal was based more on the size ratio between the cannibal and its prey than the previous activity of the potential prey. These new results could lead to solutions to decrease cannibalism in pikeperch rearing.  相似文献   

Effect of nanoparticles on crops and soil microbial communities     

Vishnu D. Rajput  Tatiana Minkina  Svetlana Sushkova  Viktoriia Tsitsuashvili  Saglara Mandzhieva  Andrey Gorovtsov  Dina Nevidomskyaya  Natalya Gromakova 《Journal of Soils and Sediments》2018,18(6):2179-2187

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Features of accumulation,migration, and transformation of benzo[a]pyrene in soil-plant system in a model condition of soil contamination     

Sushkova  Svetlana  Minkina  Tatiana  Deryabkina  Irina  Mandzhieva  Saglara  Zamulina  Inna  Bauer  Tatiana  Vasilyeva  Galina  Antonenko  Elena  Rajput  Vishnu  K?z?lkaya  R?dvan 《Journal of Soils and Sediments》2018,18(6):2361-2367

Journal of Soils and Sediments - The investigation of accumulation, migration, and transformation features of benzo[a]pyrene (BaP) in a soil-plant system by using new ecologically friendly...  相似文献