首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   115篇
  免费   7篇
林业   1篇
农学   1篇
基础科学   2篇
  1篇
综合类   10篇
畜牧兽医   103篇
植物保护   4篇
  2023年   1篇
  2021年   2篇
  2020年   1篇
  2019年   1篇
  2018年   1篇
  2017年   2篇
  2016年   4篇
  2015年   4篇
  2014年   4篇
  2013年   8篇
  2012年   4篇
  2011年   2篇
  2010年   3篇
  2009年   3篇
  2008年   6篇
  2007年   11篇
  2006年   4篇
  2005年   4篇
  2004年   2篇
  2003年   4篇
  2002年   2篇
  2001年   2篇
  2000年   5篇
  1999年   2篇
  1998年   6篇
  1997年   5篇
  1996年   5篇
  1995年   3篇
  1993年   5篇
  1992年   4篇
  1991年   2篇
  1990年   5篇
  1989年   2篇
  1988年   3篇
排序方式: 共有122条查询结果,搜索用时 15 毫秒
51.
Wide-angle seismic data along the Mantle Electromagnetic and Tomography (MELT) arrays show that the thickness of 0.5- to 1. 5-million-year-old crust of the Nazca Plate is not resolvably different from that of the Pacific Plate, despite an asymmetry in depth and gravity across this portion of the East Pacific Rise. Crustal thickness on similarly aged crust on the Nazca plate near a magmatically robust part of the East Pacific Rise at 17 degrees15'S is slightly thinner (5.1 to 5.7 kilometers) than at the 15 degrees55'S overlapping spreading center (5.8 to 6.3 kilometers). This small north-south off-axis crustal thickness difference may reflect along-axis temporal variations in magma supply, whereas the across-axis asymmetry in depth and gravity must be caused by density variations in the underlying mantle.  相似文献   
52.
Action thresholds as decision tools for insecticide application were developed and tested against the major insect pests of rice at four sites in the Philippines over a 13-year period. Action threshold treatments were compared to the farmers' practice, prophylactic insecticide usage, and an untreated check. Yield loss data using the insecticide check method partitioned yield losses over three crop growth stages in the same test fields. Chronic pests that exceeded action thresholds in 79% of fields were whorl maggot Hydrellia philippina Ferino (Diptera: Ephydridae), defoliators Naranga aenescens Moore and Rivula atimeta (Swinhoe) (Lepidoptera: Noctuidae), leaffolders Cnaphalocrocis medinalis (Guenée) and Marasmia patnalis Bradley (Lepidoptera: Pyralidae), and stemborers Scirpophaga incertulas (Walker) and S. innotata (Walker) (Lepidoptera: Pyralidae). Minor chronic pests reached threshold levels in only one site each: rice bug Leptocorisa oratorius (F.) (Koronadal), whitebacked planthopper Sogatella furcifera (Horvath) (Zaragoza) and green leafhopper Nephotettix virescens (Distant) (Guimba); brown planthopper Nilaparvata lugens (Stål) did not exceed a threshold in any field. Stemborers were the most important pest group in terms of yield loss. Despite the insecticide check method underestimating losses, a mean crop loss of 0.62 t/ha was measured which showed ample scope for corrective action. But loss was evenly distributed across crop growth stages (0.15?–?0.24 t/ha) reducing the impact of insecticides. Action threshold treatments overall outyielded the untreated check, more so in the two sites with highest pest density. The benefit of thresholds was to reduce insecticide usage, as a cost saving. However all the practices showed poor economic returns including the farmers' practice. Farmers' practice employed low insecticide dosages and timing was not consistent with pest damage, but yields were often similar to threshold treatments. Farmers appear to use insecticide more for risk aversion than for profit. The best threshold characters when evaluated against resulting pest density and yield loss criteria showed accuracies >?90% correct decisions. Future work is needed to improve the insecticide response rather than monitoring tools. Thresholds need to be incorporated into improved crop management, which was often found suboptimal by farmers, to take advantage of the high levels of tolerance in modern high tillering cultivars. Crop husbandry practices which improve yield potential such as selection of longer maturing varieties and nitrogen fertilizer may be a more effective pest management strategy than insecticides.  相似文献   
53.
The influence of weather on the population dynamics of Oebalus poecilus, the principal insect pest of rice in South and Central America, is examined. Light trapping was used for one year, but was soon discarded in favour of the more reliable sweep netting which was utilised to monitor populations in fields and non-cropped habitats from autumn 1999 to spring 2002. The results indicate that O. poecilus feeds on alternative host grasses on levées surrounding the rice fields. Adult bugs move into the fields in mid-January and mid-August of each year. In fields there are typically four generations per season, which are approximately four weeks apart. Adults move off the crop in April and October. Data presented here indicate that rainfall is critical to the ‘off-season’ survival of O. poecilus, and high rainfall during April to July and November to January causes increases in O. poecilus populations. This is particularly important given the recent extreme perturbations in the normal bimodal rainfall patterns caused by El Niño and La Niña events. The ability to predict the severity of O. poecilus populations from weekly rainfall data will provide a significant input into the development of IPM programme for O. poecilus.  相似文献   
54.
55.
This study was carried out to investigate the effect of supplementing culture medium with different concentrations of taurine and melatonin, on buffalo oocyte in vitro meiotic maturation and embryo development. In experiment 1, oocytes were matured in vitro and the cleaved embryos were cultured in the same following seven culture medium; (i) control (TCM 199 + 10% SS); (ii) control + 0.5 m m taurine; (iii) control + 1 m m taurine; (iv) control + 3 m m taurine; (v) control + 5 μ m melatonin; (vi) control + 10 μ m melatonin and (vii) control + 50 μ m melatonin. In experiment 2, based on the results of experiment 1, to examine the synergistic effect of antioxidants, the oocytes were matured in culture medium (TCM199 + 10% SS), supplemented with both taurine at 1 m m and melatonin at 10 μ m concentration and the cleaved embryos were cultured in the same medium. Supplementation of taurine at 1 m m concentration in the culture medium resulted in a higher (p < 0.05) transferable embryo (TE) yield when compared with control (20.6% vs 14.1%). Supplementation of melatonin at 10 and 50 μ m concentration in the culture medium resulted in a higher (p < 0.05) meiotic maturation rate (90.3% and 88.8% respectively) and TE yield (28.4% and 27.2% respectively), than the other treatments. In experiment 2, the TE yield did not improve by supplementing the culture medium with both taurine and melatonin, when compared with melatonin alone. In conclusion, the results of this study demonstrated that, enriching the culture medium with taurine and melatonin, improves in vitro embryo production efficiency in buffaloes. In particular, a high TE yield was obtained by enriching the culture medium with 10 μ m melatonin.  相似文献   
56.
This investigation assessed the susceptibility of experimentally infected pigeons to the highly pathogenic avian influenza virus (HPAIV) H5N1 that caused recent outbreaks of avian influenza in birds and humans in several countries of Asia. For this purpose 14 pigeons were infected ocularly and nasally with 10(8) EID50 and clinical signs were recorded and compared with five chickens infected simultaneously as positive controls. The chickens demonstrated anorexia, depression, and 100% mortality within 2 days postinoculation. Three of the pigeons died after a history of depression and severe neurological signs consisting of paresis to paralysis, mild enteric hemorrhage, resulting in a mortality of 21%. Gross lesions in these pigeons were mild and inconsistent. Occasionally subcutaneous hyperemia and hemorrhage and cerebral malacia were observed. Microscopic lesions and detection of viral antigen were confined to the central nervous system of these pigeons. In the cerebrum and to a minor extent in the brain stem a lymphohistiocytic meningoencephalitis with disseminated neuronal and glial cell necrosis, perivascular cuffing, glial nodules, and in one bird focally extensive liquefactive necrosis could be observed. The remaining nine pigeons showed neither clinical signs nor gross or histological lesions associated with avian influenza, although seroconversion against H5 indicated that they had been infected. These results confirm that pigeons are susceptible to HPAIV A/chicken/Indonesia/2003 (H5N1) and that the disease is associated with the neurotropism of this virus. Although sentinel chickens and most pigeons did not develop disease, further experiments have to elucidate whether or not Columbiformes are involved in transmission and spread of highly pathogenic avian influenza.  相似文献   
57.
Nineteen cutaneous and mucocutaneous papillomas, as well as 29 oral and 25 non-oral squamous cell carcinomas of dogs were analyzed immunohistologically for the presence of papillomavirus (PV)-antigens. Canine oral papillomavirus (COPV)-DNA was detected in formalin-fixed, paraffin-embedded tissues by polymerase chain reaction (PCR) and non-radioactive in situ hybridization (ISH). Furthermore, the expression of the tumor suppressor protein p53 was investigated. PV-antigens were detectable in more than 50% of the oral and cutaneous papillomas, while no PV-antigens could be demonstrated in venereal papillomas. One squamous cell carcinoma was PV-antigen positive. Only two cutaneous papillomas of the head showed a strong p53-specific immunostaining, while overexpressed p53 was detectable in approximately 35% of all squamous cell carcinomas. It was possible to amplify fragments of the E6, E7 and L1 gene by polymerase chain reaction (PCR) from five of eight oral and from five of eight cutaneous papillomas as well as from three oral squamous cell carcinomas. Nine of 10 papillomas showed a strong nucleus-associated hybridization signal typical for COPV-DNA. In three squamous cell carcinomas COPV-DNA was located in nests of the epithelial tumor cells surrounding ‘horn pearls' or disseminated in the carcinoma tissue. These observations support the view that COPV may also induce non-oral papillomas in the dog and confirm the opinion that a progression of viral papillomas into carcinomas in dogs may occur.  相似文献   
58.
59.
The objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (Bubalus bubalis) ovary using classical histology and nick end labelling technique. Ovaries (n = 40) procured from the slaughterhouse were used for the study. The sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end labelling (TUNEL) and classical histology (H&E). Those follicles showing ≥ 5% TUNEL positivity (TUNEL assay) and pyknotic nuclei (histology) in granulosa cells were classified as atretic. Based on histology, the atretic primary and secondary follicles (%) were 93.82 and 95.62 respectively. The histology study reveals that the rates (%) of atresia in <1, 1–3, 3–5 mm and >5 mm were 36.90, 40.50, 62.84 and 74.5 respectively. Further the atretic tertiary follicles (%) were significantly lower than the primary and secondary classes of follicles. TUNEL assay reveals that the atretic rate (%) of tertiary follicles in <1, 1–3, 3–5 and ≥ 5 mm class follicles were 50.88, 53.84, 81.81 and 36.36 respectively. The percentage of atresia in >5 mm diameter follicles were significantly lower in TUNEL than histology. Percentages of granulosa and thecal cells positive for atresia by TUNEL were 30.7 ± 0.53 and 13.82 ± 0.18 respectively per follicle. The initial structural changes in atretic follicles were seen primarily in the granulosa cells. In severely atretic follicles TUNEL positive granulosa cells along with theca cells have to be considered in assessing the rate and extent of atresia.  相似文献   
60.
Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号