Agreement between direct, oscillometric and Doppler ultrasound blood pressures using three different cuff positions in anesthetized dogs     

Garofalo NA  Teixeira Neto FJ  Alvaides RK  de Oliveira FA  Pignaton W  Pinheiro RT 《Veterinary anaesthesia and analgesia》2012,39(4):324-334

ObjectiveTo evaluate the agreement between invasive blood pressure (IBP) and Doppler ultrasound blood pressure (DUBP) using three cuff positions and oscillometric blood pressure (OBP) in anesthetized dogs.Study designProspective study.AnimalsNine adult dogs weighing 14.5–29.5 kg.MethodsThe cuff was placed above and below the tarsus, and above the carpus with the DUBP and above the carpus with the OBP monitor. Based on IBP recorded via a dorsal pedal artery catheter, conditions of low, normal, and high systolic arterial pressures [SAP (mmHg) <90, between 90 and 140, and >140, respectively] were induced by changes in isoflurane concentrations and/or dopamine administration. Mean biases ± 2 SD (limits of agreement) were determined.ResultsAt high blood pressures, regardless of cuff position, SAP determinations with the DUBP underestimated invasive SAP values by more than 20 mmHg in most instances. With the DUBP, cuff placement above the tarsus yielded better agreement with invasive SAP during low blood pressures (0.2 ± 16 mmHg). The OBP underestimated SAP during high blood pressures (?42 ± 42 mmHg) and yielded better agreement with IBP for mean (MAP) and diastolic (DAP) arterial pressure measurements [overall bias: 2 ± 15 mmHg (MAP) and 0.2 ± 16 mmHg (DAP)].ConclusionsAgreement of SAP determinations with the DUBP is poor at SAP > 140 mmHg, regardless of cuff placement. Measurement error of the DUBP with the cuff placed above the tarsus is clinically acceptable during low blood pressures. Agreement of MAP and DAP measurements with this OBP monitor compared with IBP was clinically acceptable over a wide pressure range.Clinical relevanceWith the DUBP device, placing the cuff above the tarsus allows reasonable agreement with IBP obtained via dorsal pedal artery catheterization. Only MAP and DAP provide reasonable estimates of direct blood pressure with the OBP monitor evaluated.  相似文献   

Rubber tree early selection for yield stability in time and among locations     

Lígia Regina Lima Gouvêa  Guilherme Augusto Peres Silva  Cecília Khusala Verardi  André Luis Bombonato de Oliveira  Elaine Cristine Piffer Gonçalves  Erivaldo José Scaloppi-Junior  Mário Luiz Teixeira de Moraes  Paulo de Souza Gonçalves 《Euphytica》2013,191(3):365-373

Rubber production in the rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] can be expressed differently in different environments. Thus the objective of the present study was to select productive progenies, stable and responsive in time and among locations. Thirty progenies were assessed by early yield tests at three ages and in three locations. A randomized block design was used with three replications and ten plants per plot, in 3 × 3 m spacing. The procedure of the mixed linear Reml/Blup model—restricted maximum likelihood/best non-biased linear prediction was used in the genetic statistical analyses. In all the individual analyses, the values observed for the progeny average heritability ( $ \hat{h}_{pa}^{2} $ ) were greater than those of the additive effect based on single individuals ( $ \hat{h}_{a}^{2} $ ) and within plot additive ( $ \hat{h}_{ad}^{2} $ ). In the joint analyses in time, there was genotype × test interaction in the three locations. When 20 % of the best progenies were selected the predicted genetic gains were: Colina G_G = 24.63 %, Selvíria G_G = 13.63 %, and Votuporanga G_G = 25.39 %. Two progenies were among the best in the analyses in the time and between locations. In the joint analysis among locations there was only genotype × location interaction in the first early test. In this test, selecting 20 %, the general predicted genetic gain was G_G = 25.10 %. Identifying progenies with high and stable yield over time and among locations contributes to the efficiency of the genetic breeding program. The relative performance of the progenies varies depending of the age of early selection test.  相似文献   

Follicular degeneration in the ovaries of goats experimentally infected with Trypanosoma vivax from the Brazilian semi-arid region     

Carla M.F. Rodrigues  Roberio G. Olinda  Taciana M.F. Silva  Rodolfo G. Vale  Anderson E. da Silva  Gabriela L. Lima  Herakles A. Garcia  Marta M.G. Teixeira  Jael S. Batista 《Veterinary parasitology》2013,191(1-2):146-153

Infection by Trypanosoma vivax and other African trypanosomes plays an important role in reproductive disorders in male and female livestock. Outbreaks of T. vivax in the semi-arid region of northeastern Brazil are characterized by wasting disease in cattle, sheep and goats with hematological, cardiac and nervous compromises in addition to reproductive failures. Similar to reports from Africa, we previously observed a reduction in fertility rates and severe testicular degeneration and epididymitis in male sheep infected with T. vivax from this region. Although anestrus is frequently reported in goats and sheep infected with T. vivax, the effects of this infection on the female reproductive organs need clarification. In this study, we addressed this issue through a histopathological evaluation of ovarian follicular morphology and classification in goats experimentally infected with a T. vivax isolate from the Brazilian semi-arid region. The infected animals presented typical clinical signs of trypanosomosis by T. vivax, including anemia, hyperthermia, pallor of the mucous membranes, enlarged lymph nodes, and progressive loss of weight. All the infected goats remained anestrus throughout the experimental period and exhibited important disturbances in the ovaries, evidenced by reduced size and a smooth surface without follicles or corpora lutea, and abnormal follicular development. In addition, through PCR, we detected T. vivax DNA in the ovarian tissues of the infected goats. Our findings contributed to understand the female reproductive failure associated with trypanosomosis caused by T. vivax.  相似文献   

Lithothamnion muelleri Controls Inflammatory Responses,Target Organ Injury and Lethality Associated with Graft-versus-Host Disease in Mice     

Barbara M. Rezende  Priscila T. T. Bernardes  Carolina B. Resende  Rosa M. E. Arantes  Danielle G. Souza  Fern?o C. Braga  Marina G. M. Castor  Mauro M. Teixeira  Vanessa Pinho 《Marine drugs》2013,11(7):2595-2615

Lithothamnion muelleri (Hapalidiaceae) is a marine red alga, which is a member of a group of algae with anti-inflammatory, antitumor, and immunomodulatory properties. The present study evaluated the effects of treatment with Lithothamnion muelleri extract (LM) in a model of acute graft-versus-host disease (GVHD), using a model of adoptive splenocyte transfer from C57BL/6 donors into B6D2F1 recipient mice. Mice treated with LM showed reduced clinical signs of disease and mortality when compared with untreated mice. LM-treated mice had reduced tissue injury, less bacterial translocation, and decreased levels of proinflammatory cytokines and chemokines (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-C motif) ligand 3 (CCL3) and chemokine (C-C motif) ligand 5 (CCL5)). The polysaccharide-rich fraction derived from LM could inhibit leukocyte rolling and adhesion in intestinal venules, as assessed by intravital microscopy. LM treatment did not impair the beneficial effects of graft-versus-leukaemia (GVL). Altogether, our studies suggest that treatment with Lithothamnion muelleri has a potential therapeutic application in GVHD treatment.  相似文献   

Culture results from milk samples submitted to veterinary diagnostic laboratories from August 2003 to December 2006 in New Zealand     

KR Petrovski  NB Williamson  N Lopez-Villalobos  TJ Parkinson  IG Tucker 《New Zealand veterinary journal》2013,61(6):317-322

Abstract

AIMS: To determine the pattern of isolation of major mastitis-causing organisms isolated from milk samples submitted to five veterinary diagnostic laboratories in New Zealand.

METHODS: The culture results of 25,288 milk samples that were collected from dairy cows throughout New Zealand from August 2003 to December 2006 and submitted to a group of veterinary diagnostic laboratories were assembled, reviewed and summarised. Logistic regression was used to analyse the effect of year, region (i.e. North vs South Island), and season on the probability of isolating the two most common organisms.

RESULTS: The most commonly isolated mastitis causing organisms from all samples were: Streptococcus uberis (23.6%), Staphylococcus aureus (23.5%), coagulase-negative staphylococci (CNS; 7.2%), Strep. dysgalactiae (6.2%), Bacillus spp. (4.0%), and coliforms (3.7%). The percentage of samples with isolates of Strep. uberis or Staph. aureus was affected by island, year and season (p<0.001). For most of the year, except in late winter and early spring when Strep. uberis was much more common, the percentage of isolates of Strep. uberis and Staph. aureus were not apparently different despite the former being an environmental pathogen and the other a contagious one.

CONCLUSION: The pattern of isolation of major mastitis-causing organisms, as determined from culture of milk samples submitted to diagnostic laboratories in New Zealand, has changed significantly over the last 40 years, with a substantial increase in the percentage of isolates that are Strep. uberis and a decrease in isolates of Strep. agalactiae. There is a clear seasonal pattern to the isolation of both Strep. uberis and Staph. aureus, particularly the former.

CLINICAL RELEVANCE: Knowledge of the aetiological agents causing bovine mastitis on a farm is of value in determining the choice of treatment. This dataset shows that, although there is seasonal pattern to the isolation of mastitis-causing organisms in New Zealand, both Strep. uberis and Staph. aureus are isolated throughout the year, so bacteriology is of value in determining aetiology even in late winter/early spring.  相似文献   

Pathogenesis of reproductive failure induced by Trypanosoma vivax in experimentally infected pregnant ewes     

Taciana MF Silva  Roberio G Olinda  Carla MF Rodrigues  Ant?nio CL Camara  Francisco C Lopes  Wesley AC Coelho  Múcio FB Ribeiro  Carlos IA Freitas  Marta MG Teixeira  Jael S Batista 《Veterinary research》2013,44(1):1

The present study was aimed at investigating the effect of experimental infection by Trypanosoma vivax in different stages of pregnancy, determining the pathogenesis of reproductive failure, and confirming transplacental transmission. We used 12 pregnant ewes distributed into four experimental groups: G1, was formed by three ewes infected with T. vivax in the first third of pregnancy (30 days); G2 comprised three infected ewes in the final third of pregnancy (100 days); G3 and G4 were composed of three non-infected ewes with the same gestational period, respectively. Each ewe of G1 and G2 was inoculated with 1.25 × 10⁵ tripomastigotes. Clinical examination, determination of parasitemia, serum biochemistry (albumin, total protein, glucose, cholesterol, and urea), packed cell volume (PCV), serum progesterone, and pathological examination were performed. Placenta, amniotic fluid, blood and tissues from the fetuses and stillbirths were submitted to PCR. Two ewes of G1 (Ewe 1 and 3) presented severe infection and died in the 34^th and 35^th days post-infection (dpi), respectively; but both fetuses were recovered during necropsy. In G2, Ewe 5 aborted two fetuses on the 130^th day (30 dpi) of pregnancy; and Ewe 6 aborted one fetus in the 140^th day (40 dpi) of gestation. Ewes 2 and 4 delivered two weak lambs that died five days after birth. Factors possibly involved with the reproductive failure included high parasitemia, fever, low PCV, body score, serum glucose, total protein, cholesterol, and progesterone. Hepatitis, pericarditis, and encephalitis were observed in the aborted fetuses. The presence of T. vivax DNA in the placenta, amniotic fluid, blood, and tissues from the fetuses confirms the transplacental transmission of the parasite. Histological lesion in the fetuses and placenta also suggest the involvement of the parasite in the etiopathogenesis of reproductive failure in ewes.  相似文献   

Extracts of Peels and Seeds of Five Varieties of Brazilian Jabuticaba Present High Capacity to Deactivate Reactive Species of Oxygen and Nitrogen     

Paludo  Michelly Cristiane  de Oliveira  Luciana Fontes  Hermosín-Gutiérrez  Isidro  Ballus  Cristiano Augusto  Ribeiro  Alessandra Braga  de Oliveira  Silvia Borges Pimentel  Godoy  Helena Teixeira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(1):135-140

Plant Foods for Human Nutrition - Jabuticaba has a high concentration of phenolic compounds, which have a significant antioxidant capacity. Methodologies have been developed to evaluate the ability...  相似文献   

Impact of fertilization on cover crops and microbial community on a bauxite‐mined soil undergoing reclamation     

Rafael S. Teixeira  Ivan F. Souza  Rafael S. Santos  Lucas C. Gomes  Silvano R. Borges  Leonardus Vergütz  Ivo R. Silva 《植物养料与土壤学杂志》2019,182(4):515-523

The reclamation of bauxite‐mined areas can be favored by the application of organic and/or chemical fertilization to restore the vegetation. Otherwise, the impact of fertilizations on soil microbiota or plant–microbe interactions as land reclamation progresses is less understood. To address this issue, we evaluated the impact of organic and chemical fertilization on plants and soil microbial community within the first 36 months of land reclamation in a bauxite‐mined site. The experiment was set up according to a split‐plot design in which the main plots received fertilizations [non‐fertilized control (NF), chemical fertilization (CF; NPK and rock phosphate), organic fertilization (OF; poultry litter), and CF+OF combined], and the subplots received cover crops [no cover crops (NC), grass (B; Brachiaria), legume (S, Stylosanthes), and B+S combined]. Cover crops biomass yield was assessed annually with five field campaigns per year. We used phospholipid fatty acids (PLFAs) to infer the impacts of mining and restoration practices on actinobacteria, Gram‐negative and Gram‐positive bacteria, arbuscular mycorrhizal fungi, and fungi. Accordingly, PLFAs were determined before bauxite mining (pre‐mining), six months after topsoil reconfiguration (post‐mining), and after 14 and 36 months following the application of the fertilizations and cover crops. PLFAs results indicated that in post‐mining, the living microbiota was significantly lower than in pre‐mining. Cover crops biomass yield was highest for B and B+S fertilized with CF+OF at 14 and 36 months. Both parametric and non‐parametric statistics showed a temporal variation in the response of living microbes to the treatments applied. After 14 months, living microbes showed greatest response to OF, while at 36 months their response was strongest in the treatments with highest plant biomass production (B and B+S). These results suggest that in the early stages of land reclamation, living microbial biomass benefit the most from organic fertilizers. As this initial boost decline, living microbes are more likely to thrive in areas undergoing reclamation where they can develop synergistic interactions with plants.  相似文献   

Nitrogen retention and plant uptake on a highly weathered central Amazonian Ferralsol amended with compost and charcoal     

Christoph Steiner  Bruno Glaser  Wenceslau Geraldes Teixeira  Johannes Lehmann  Winfried E.H. Blum  Wolfgang Zech 《植物养料与土壤学杂志》2008,171(6):893-899

Leaching losses of N are a major limitation of crop production on permeable soils and under heavy rainfalls as in the humid tropics. We established a field trial in the central Amazon (near Manaus, Brazil) in order to study the influence of charcoal and compost on the retention of N. Fifteen months after organic‐matter admixing (0–0.1 m soil depth), we added ¹⁵N‐labeled (NH₄)₂SO₄ (27.5 kg N ha^–1 at 10 atom% excess). The tracer was measured in top soil (0–0.1 m) and plant samples taken at two successive sorghum (Sorghum bicolor L. Moench) harvests. The N recovery in biomass was significantly higher when the soil contained compost (14.7% of applied N) in comparison to only mineral‐fertilized plots (5.7%) due to significantly higher crop production during the first growth period. After the second harvest, the retention in soil was significantly higher in the charcoal‐amended plots (15.6%) in comparison to only mineral‐fertilized plots (9.7%) due to higher retention in soil. The total N recovery in soil, crop residues, and grains was significantly (p < 0.05) higher on compost (16.5%), charcoal (18.1%), and charcoal‐plus‐compost treatments (17.4%) in comparison to only mineral‐fertilized plots (10.9%). Organic amendments increased the retention of applied fertilizer N. One process in this retention was found to be the recycling of N taken up by the crop. The relevance of immobilization, reduced N leaching, and gaseous losses as well as other potential processes for increasing N retention should be unraveled in future studies.  相似文献   

Synthesis and phytotoxic activity of ozonides     

Barbosa LC  Pereira UA  Teixeira RR  Maltha CR  Fernandes SA  Forlani G 《Journal of agricultural and food chemistry》2008,56(20):9434-9440

The [4 + 3] cycloaddition of the proper furans with the oxyallyl cation, generated in situ from 2,4-dibromopentan-3-one, produced a series of 8-oxabicyclo [3.2.1]oct-6-en-3-ones. Exposure of the oxabicycles to ozone afforded the corresponding 8,9,10,11-tetraoxatricyclo[5.2.1.1 (2,6)]undecan-4-ones in variable yields (7-100%). The phytotoxic properties of these ozonides (or 1,2,4-trioxolanes) and their oxabicycle precursors were evaluated as the ability to interfere with the growth of Sorghum bicolor and Cucumis sativus seedlings. Among oxabicycles, the highest inhibitory activity was shown by compounds possessing a alpha,beta-unsaturated carbonyl moiety. A differential sensitivity of the two crops was evident with ozonides. The most active compounds were also tested against the weed species Ipomoea grandifolia and Brachiaria decumbens. To the best of our knowledge, this is the first article describing ozonides as potential herbicides.  相似文献