Production of Hyaluronic Acid by Streptococcus zooepidemicus on Protein Substrates Obtained from Scyliorhinus canicula Discards     

José A. Vázquez  Lorenzo Pastrana  Carmen Pi?eiro  José A. Teixeira  Ricardo I. Pérez-Martín  Isabel R. Amado 《Marine drugs》2015,13(10):6537-6549

This work investigates the production of hyaluronic acid (H) by Streptococcus equi subsp. zooepidemicus in complex media formulated with peptones obtained from Scyliorhinus canicula viscera by-products. Initially, in batch cultures, the greatest productions were achieved using commercial media (3.03 g/L) followed by peptones from alcalase hydrolyzed viscera (2.32 g/L) and peptones from non-hydrolyzed viscera (2.26 g/L). An increase of between 12% and 15% was found in subsequent fed-batch cultures performed on waste peptones. Such organic nitrogen sources were shown to be an excellent low-cost substrate for microbial H, saving more than 50% of the nutrient costs.  相似文献   

Low genetic variability in Sclerotinia sclerotiorum populations from common bean fields in Minas Gerais State,Brazil, at regional,local and micro‐scales          下载免费PDF全文

M. S. Lehner  T. J. Paula Júnior  B. T. Hora Júnior  H. Teixeira  R. F. Vieira  J. E. S. Carneiro  E. S. G. Mizubuti 《Plant pathology》2015,64(4):921-931

Sclerotinia sclerotiorum, causal agent of white mould, is the most destructive and widely distributed soilborne pathogen of common bean during the autumn–winter season in Brazil. Nevertheless, little is known about the genetic structure of the pathogen population. Microsatellite (SSR) markers and mycelial compatibility groups (MCGs) were used to characterize 118 isolates collected from 20 bean fields located in the most important growing regions of Minas Gerais State (MG). Additionally, the genetic variability among 10 isolates obtained from a single sclerotium was investigated in 10 different sclerotia. Seventy SSR haplotypes and 14 MCGs were identified among the 118 isolates. The genetic differences within bean growing areas accounted for most of the genetic variation (72%). Despite the relatively high genotypic diversity, the SSR loci were at linkage disequilibrium. Moreover, 70% of the isolates were assigned to only two MCGs, and haplotypes of a given MCG were closely related. The discriminant analysis of principal components revealed five groups. There was strong genetic differentiation between isolates collected in one municipality in southern MG when compared to other regions. Common bean resistance to white mould should be assessed with representative isolates of the five genetic groups and, if possible, of the different MCGs detected in the present study. One to five haplotypes were detected among the 10 isolates obtained from a single sclerotium. Therefore, in order to ensure genetic identity of an isolate, hyphal tip or monoascosporic isolates should be used.  相似文献   

Silver catfish Rhamdia quelen immersion anaesthesia with essential oil of Aloysia triphylla (L'Hérit) Britton or tricaine methanesulfonate: effect on stress response and antioxidant status     

Luciane Tourem Gressler  Ana Paula Konzen Riffel  Thaylise Vey Parodi  Etiane Medianeira Hundertmarck Saccol  Gessi Koakoski  Sílvio Teixeira da Costa  Maria Amália Pavanato  Berta Maria Heinzmann  Bráulio Caron  Denise Schmidt  Susana Francisca Llesuy  Leonardo José Gil Barcellos  Bernardo Baldisserotto 《Aquaculture Research》2014,45(6):1061-1072

Responses to anaesthesia with essential oil (EO) of Aloysia triphylla (135 and 180 mg L^?1) and tricaine methanesulfonate (MS222) (150 and 300 mg L^?1) were assessed in silver catfish. Exposure to the anaesthetics elicited a stress response in the species. In the case of MS222, it was displayed as a release of cortisol into bloodstream, elevation in hematocrit and plasma ion loss. The EO presented cortisol‐blocking properties, but increased haematocrit and disturbances of hydromineral balance were observed. Liver antioxidant/oxidant status of EO and MS222‐anaesthetized silver catfish was also estimated. The synthetic anaesthetic induced lipoperoxidation, notwithstanding increased catalase contents, whereas the naturally occurring product was capable of preventing the formation of lipid peroxides, possibly due to combined actions of catalase and glutathione‐S‐transferase. Anaesthetic efficacy was also tested via induction and recovery times. Overall, the promising results obtained for the physiological parameters of the EO‐treated fish counterbalanced the slight prolonged induction time observed for 180 mg L^?1. As for 135 mg L^?1, both induction and recovery times were lengthy; despite that, the EO was able to promote oxidative protection and mitigate stress. None of the MS222 concentrations prompted such responses concomitantly.  相似文献   

Portuguese traditional grapevine cultivars and wild vines (Vitis vinifera L.) share morphological and genetic traits     

J. Cunha  M. Teixeira Santos  L. C. Carneiro  P. Fevereiro  J. E. Eiras-Dias 《Genetic Resources and Crop Evolution》2009,56(7):975-989

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A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles     

de Carvalho RA  Torres TT  de Azeredo-Espin AM 《Veterinary parasitology》2006,140(3-4):344-351

Cochliomyia hominivorax (Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. In Brazil, C. hominivorax has been controlled mainly with organophosphate (OP) insecticides, although the inappropriate use of these chemicals can result in the selection of resistant flies. Changes in carboxylesterase activity have been associated with OP insecticides in some arthopodan species. In this work, we isolated and characterized part of the E3 gene in C. hominivorax (ChE7), which contained the same substitutions responsible for the acquisition of OP hydrolase activity in Lucilia cuprina (Calliphoridae). Digestion of the polymerase chain reaction products with a restriction enzyme that specifically recognized the mutation site unambiguously differentiated wild and mutated esterase alleles. The PCR-RFLP assay therefore provided a fast, reliable DNA-based method for identifying C. hominivorax individuals with a mutation in the esterase gene. Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species.  相似文献   

Fiscal Decentralization and Economic Growth in Central and Eastern Europe     

ANDRÉS RODRÍGUEZ‐POSE  ANNE KRØIJER 《Growth and change》2009,40(3):387-417

The majority of the literature on fiscal decentralization has tended to stress that the greater capacity of decentralized governments to tailor policies to local preferences and to be innovative in the provision of policies and public services, the greater the potential for economic efficiency and growth. There is, however, little empirical evidence to substantiate this claim. In this paper we examine, using a panel data approach with dynamic effects, the relationship between the level of fiscal decentralization and economic growth rates across 16 Central and Eastern European countries over the 1990–2004 period. Our findings suggest that, contrary to the majority view, there is a significant negative relationship between two out of three fiscal decentralization indicators included in the analysis and economic growth. However, the use of different time lags allows us to nuance this negative view and show that long‐term effects vary depending on the type of decentralization undertaken in each of the countries considered. While expenditure at and transfers to sub‐national tiers of government are negatively correlated with economic growth, taxes assigned at the sub‐national level evolve from having a significantly negative to a significantly positive correlation with the national growth rate. This supports the view that sub‐national governments with their own revenue source respond better to local demands and promote greater economic efficiency  相似文献   

Five methods to interpret field measurements of energy fluxes over a micro-sprinkler-irrigated mango orchard     

A. H. de C. Teixeira  W. G. M. Bastiaanssen 《Irrigation Science》2012,30(1):13-28

Energy balance measurements were carried out in a mango orchard during two growing seasons in the semi-arid region of Brazil. The actual evapotranspiration (ET) was acquired by eddy correlation (EC) and Bowen ratio energy balance (BR) techniques. The daily energy balance closure in the EC measurements showed an average gap of 12%, with a root mean square error (RMSE) of 1.7 MJ m⁻² d⁻¹. Three different correction procedures were tested for closing the energy balance from the EC system: (1) the surface energy balance residual method (RES), (2) the Bowen ratio determined from the EC fluxes, the combination approach (EC_BR), and (3) a new regression energy balance closure technique (REG). All closing energy balance methods presented good correlation with the direct EC measurements, but the trends were not similar. The latent heat fluxes estimated by the BR method—λE _BR—were higher than those from the direct EC measurements—λE _EC. When using the RES method, the half-hour λE _EC measurements represented around 88% of the λE _RES values, as the uncertainties of net radiation—R _n—and soil heat fluxes—G—are propagated into the RES method. The latent heat flux derived from the combination approach—λE _{EC_BR}—also brings these uncertainties, being the agreements comparable with those for RES method. It was therefore concluded that a single correction method for EC measurements considering only the latent and sensible heat fluxes does not exist. A new way to solve the lack of energy balance closure from EC techniques was tested by means of a curve fitting, the REG method. Considering the REG corrections applied to the energy balance components involving all periods of the day and the average conditions of the two growing seasons, half-hour values of λE _EC were overmeasured by 18%, H _EC was undermeasured by 17%, and G values required a correction of 466%. The REG method appeared promising because it considers different weights for all energy balance components in the optimization process. Taking the REG results for the drier second growing season as a reference, it was concluded that seasonal ET values by the other methods in mango orchard ranged from 7 to 28% higher, showing that turbulent flux measurements lack accuracy for executing on-farm water-saving programmes and calibrating transient soil water flow models.  相似文献   

Evaluating the potential of wine-making residues and corn cobs as support materials for cell immobilization for ethanol production     

Zlatina GenishevaSolange I. Mussatto  José M. OliveiraJosé A. Teixeira 《Industrial Crops and Products》2011,34(1):979-985

Three wine-making residues (grape seeds, skins and stems), and corn cobs were evaluated as support material for immobilization of Saccharomyces cerevisiae and the ethanol production by the immobilized cells was assessed. The main objective of this study was to find an abundant and low cost material suitable for the cells immobilization and able to be used in a next step of wine production by immobilized yeast cells. The four natural materials were used as support in two different forms: untreated, and treated by a sequence of acid and basic reactions. Untreated grape skin and corn cobs provided the highest cell immobilization results (25.1 and 22.2 mg cells/g support, respectively). The maximum ethanol production yield (about 0.50 g/g) was also obtained when the cells were immobilized in these untreated materials. It was also found that the support materials released nutrients to the medium, which favored the yeast development and the ethanol production. The use of immobilized cells systems under agitated conditions gave ethanol yields similar to those obtained by the static fermentations, but the immobilized cell concentration was significantly lower. In brief, static fermentation with cells immobilized on grape skins or corn cobs appear to be an interesting alternative for use on wine-making. The use of grape skins, particularly, which is a by-product of the wine elaboration, could be of larger interest to obtain an integrated wine production process with by-product reuse.  相似文献   

Differential expression of molecular rust resistance components have distinctive profiles in <Emphasis Type="Italic">Coffea arabica</Emphasis> - <Emphasis Type="Italic">Hemileia vastatrix</Emphasis> interactions     

Geleta Dugassa Barka  Eveline Teixeira Caixeta  Robson Ferreira de Almeida  Samuel Mazzinghy Alvarenga  Laércio Zambolim 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(3):543-561

Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (non-specific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed.  相似文献   

Synthesis,theoretical studies,and effect on the photosynthetic electron transport of trifluoromethyl arylamides          下载免费PDF全文

Róbson Ricardo Teixeira  Marcus Vinícius de Andrade Barros  Gustavo Costa Bressan  Raoni Pais Siqueira  Fabíola Suelen dos Santos  Michele Bertazzini  Rudolf Kiralj  Márcia Miguel Castro Ferreira  Giuseppe Forlani 《Pest management science》2017,73(11):2360-2371

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