Seasonal Effect on Growth Parameters and Macronutrient Use of Sweet Pepper     

Maréa Prieto  Jesús Peñalosa  Ma José Sarro  Pilar Zornoza  Agustén Gárate 《Journal of plant nutrition》2013,36(11):1803-1820

ABSTRACT

Sweet pepper plants (Capsicum annuum L.) grown under greenhouse conditions differed widely in the rate of production of their total dry mass and their harvestable weight, although there was not shortage in water or nutrient supply. Plants were grown in aerated nutrient solutions along two different growing seasons. The differences in plant productivity were mainly due to the environmental conditions of the growth cycle. During the increasing day length season, from the beginning of spring to summer (13.0 to 14.8 h day^{? 1}), plants produced the largest amount of fruits, but with a 10% blossom-end rot incidence. In the season of short photoperiod, progressing from the beginning of autumn to winter with 13.3 to 10.3 light h day^{? 1}, the total dry weight and yields gradually decreased, but blossom-end rot in fruit did not appear. Different growth patterns were found for morphological and physiological parameters, because of the different light loads received by the canopy, being adjusted to the seasonal variables. Reduced light load markedly reduced leaf area and leaf thickness, but increased specific leaf area, which gave plants an increased ability to intercept light. Not only the amount of initial biomass, but radiation and the age of the pepper plants were the main growing driving factors, influencing relative growth rate, nitrogen (N)-use efficiency, and calcium (Ca) partitioning. Moreover, mathematical approaches for prediction of biomass production and N accumulation as a function of radiation are given in order to establish in the future a sweet pepper growth model.  相似文献   

A survey of Nicotiana germplasm for resistance to Tomato spotted wilt virus (TSWV)     

Dorota Laskowska  Teresa Doroszewska  Anna Depta  Karolina Kursa  Hanna Olszak-Przybyś  Anna Czubacka 《Euphytica》2013,193(2):207-219

The reaction to Tomato spotted wilt virus (TSWV) was evaluated in 94 accessions of Nicotiana, originating from the Institute of Soil Science and Plant Cultivation tobacco germplasm collection in Pu?awy, Poland. Tests for resistance were conducted under greenhouse conditions using single TSWV isolate collected from tobacco plantation in Lublin district, Poland. The presence of the virus was verified using DAS-ELISA. SCAR markers associated with TSWV resistance gene were applied. The members of the section Alatae, the genus Nicotiana: N. alata, N. forgetiana, and Nicotiana x sanderae as well as N. tabacum cultivars: ‘Polalta’ and ‘Wiktoria’ with the TSWV resistance gene introduced from N. alata, displayed the hypersensitive reaction (HR) against TSWV (grade 0 on symptom intensity scale). In some of those accessions, the virus spread from the initially infected areas eliciting systemic hypersensitive reaction (SHR). Five accessions of N. alata and three of Nicotiana x sanderae were composed of 6.3–50.0 % of plants in which SHR symptoms appeared. In all of N. forgetiana plants HR reaction was followed by systemic infection (SHR). In N. tabacum ‘Wiktoria’ 21.1 % of plants showed HR reaction, while the remaining were susceptible (S). All of the genotypes which responded with HR or SHR reaction to TSWV infection demonstrated the presence of SCAR markers linked to the resistance gene. The remaining eighty tested accessions were identified as being susceptible upon exposure to TSWV.  相似文献   

Spatial road disturbance index (SPROADI) for conservation planning: a novel landscape index, demonstrated for the State of Brandenburg, Germany     

Lisa Freudenberger  Peter R. Hobson  Slaven Rupic  Guy Pe’er  Martin Schluck  Julia Sauermann  Stefan Kreft  Nuria Selva  Pierre L. Ibisch 《Landscape Ecology》2013,28(7):1353-1369

The expansion of roads, and the subsequent changes to the surrounding landscape not only lead to landscape fragmentation but also have been shown to be a key driver of biodiversity loss and ecosystem degradation. Local declines of species abundance as well as changes in animal behaviour have drawn attention to wider ecosystem effects including altered species composition and a degradation of ecosystem functioning. However, methods for measuring and quantifying the distribution and environmental impacts of roads are not yet fully developed. We present a new technique for assessing the potential impacts of roads on biodiversity using a spatial road disturbance index (SPROADI). The index is calculated from three sub-indices: traffic intensity as a measure of traffic volume per time and space; vicinity impact, which is the assessment of edge effect of roads on adjacent habitats (the road-effect zone); and fragmentation grade, which provides an indication of the degree to which the landscape is intersected by roads. SPROADI was then tested using data from the Federal State of Brandenburg in north-eastern Germany. A sensitivity analysis was carried out on the results to assess the robustness of the index. The findings revealed expected patterns of high road disturbance in urban and peri-urban landscapes surrounding Berlin. Less obvious were the high levels of road density and impacts in forest plantations across the southern region of Brandenburg, and low levels of road disturbance in agricultural crop lands of the north-western region. Results were variable for areas under some form of protection. The only national park displayed substantially lower SPROADI values in contrast to the surrounding non-protected areas whilst other protected area categories, which were landscape conservation areas and nature parks, revealed SPROADI values that were equally high as those for non-protected areas. The results of this study demonstrate the strengths and potential applications of SPROADI as a quantitative means for identifying low-traffic areas in the context of conservation and sustainable transport planning.  相似文献   

Dimethylformamide Improves the In vitro Characteristics of Thawed Stallion Spermatozoa Reducing Sublethal Damage     

A Morillo Rodriguez  C Balao da Silva  B Macías‐García  JM Gallardo Bolaños  JA Tapia  IM Aparicio  C Ortega‐Ferrusola  FJ Peña 《Reproduction in domestic animals》2012,47(6):995-1002

A total of 42 ejaculates were used in the experiment; six ejaculates per stallion, obtained from seven Pure Spanish stallions (PRE), were split and frozen in freezing media with different concentrations and combinations of cryoprotectant (CPA): (i) Cáceres (skim milk based extender) containing 2.5% glycerol (2.5GL), (ii) Cáceres containing 1.5% glycerol and 1.5% dimethylformamide (1.5%GL–1.5%DMFA), (iii) Cáceres extender supplemented with 1.5% glycerol and 2.5% dimethylformamide (1.5%GL–2.5%DMFA) and (iv) Cáceres extender supplemented with 4% dimethylformamide (4%DMFA). After at least 4 weeks of storage in liquid nitrogen (LN), straws were thawed and semen analysed by computer‐assisted sperm analysis and flow cytometry (membrane lipid architecture (Merocyanine 540), integrity and sublethal damage (YoPro‐1) and mitochondrial membrane potential (JC‐1)). After thawing, better results were observed in samples frozen in 4%DMFA or in combinations of 1.5%GL–2.5%DMFA, in fact total motility increased by 16% in the 4%DMFA group compared to 2.5%GL (P < 0.05). Also, there was an increment in the percentage of progressive motile sperm in the 1.5%GL–2.5%DMFA group (9.8% 2.5GL vs 19% in the 1.5%GL–2.5%DMFA group p < 0.05); also, samples frozen in the 4%DMFA group had more intact (YoPro‐1 negative) sperm post‐thawing, 29.3% in 2.5%GL vs 36.7% in 4%DMFA group (p < 0.05). Membrane lipid architecture was not affected by any of the cryoprotectants tested, while samples frozen in 4%DFMA had a lower percentage of mitochondria with lower membrane potential. It is concluded that DMFA improves the outcome of cryopreservation of stallion spermatozoa mainly reducing sublethal cryodamage.  相似文献   

Canine periodontitis: the dog as an important model for periodontal studies     

Albuquerque C  Morinha F  Requicha J  Martins T  Dias I  Guedes-Pinto H  Bastos E  Viegas C 《Veterinary journal (London, England : 1997)》2012,191(3):299-305

Periodontal disease (PD) refers to a group of inflammatory diseases caused by bacterial plaque in the periodontium and ranges from an early stage (gingivitis) to an advanced stage (periodontitis). It is a multifactorial disease that results from the interaction of the host defence mechanisms with the plaque microorganisms. Early detection, diagnosis and treatment are essential in the control of this disease. PD has an enormous impact on human and veterinary medicine due to its high prevalence. The most common animal PD models use dogs and non-human primates, although other animals (rats, mice, hamsters, rabbits, miniature pigs, ferrets, and sheep) have also been employed. Dog models have contributed significantly to the current understanding of periodontology. The most important clinical aspects of canine PD are considered in this review and the various animal models are examined with an emphasis on the role of the dog as the most useful approach for understanding human PD and in the development of new therapeutic and preventive measures.  相似文献   

The membrane of the mammalian spermatozoa: much more than an inert envelope     

Tapia JA  Macias-Garcia B  Miro-Moran A  Ortega-Ferrusola C  Salido GM  Peña FJ  Aparicio IM 《Reproduction in domestic animals = Zuchthygiene》2012,47(Z3):65-75

Sperm plasma membrane is a very important structure that functions to protect sperm against extracellular injuries and to respond to physiological challenges. It plays a crucial role during sperm capacitation, in sperm-egg interaction and, finally, in fertilization. Concerning sperm technology, possibly the most important factors causing damage in mammalian spermatozoa membranes are initiated by the osmotic stress generated by dehydration of the cells during freezing and thawing. These changes are rapidly derived to the plasma and organelle membranes that gradually experiment loss of membrane architecture, causing unbalanced production of reactive oxygen species and increased lipid peroxidation. Other procedures such as sperm sorting or liquid storage of sperm also induce harmful changes in the integrity of the membrane. The specific composition of lipids of the sperm membranes may provide clues for understanding the mechanisms behind the differences found in the response to stress in different species. In the present review, we deal with the composition, architecture and organization of the sperm plasma membrane, emphasizing the factors that can affect membrane integrity. The intracellular signalling pathways related with membrane reorganization during capacitation and acrosome reaction are also reviewed.  相似文献   

Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs by leucine through an mTORC1-dependent pathway     

Agus Suryawan  Hanh V Nguyen  Rosemarie D Almonaci  Teresa A Davis 《畜牧与生物技术杂志(英文版)》2012,3(1):32-41

Neonatal growth is characterized by a high protein synthesis rate that is largely due to an enhanced sensitivity to the postprandial rise in insulin and amino acids, especially leucine. The mechanism of leucine’s action in vivo is not well understood. In this study, we investigated the effect of leucine infusion on protein synthesis in skeletal muscle and liver of neonatal pigs. To evaluate the mode of action of leucine, we used rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) complex-1 (mTORC1). Overnight-fasted 7-day-old piglets were treated with rapamycin for 1 hour and then infused with leucine (400 μmol·kg -1 ·h -1 ) for 1 hour. Leucine infusion increased the rate of protein synthesis, and ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) phosphorylation in gastrocnemius and masseter muscles (P < 0.05), but not in the liver. The leucine-induced stimulation of protein synthesis and S6K1 and 4E-BP1 phosphorylation were completely blocked by rapamycin, suggesting that leucine action is by an mTORC1-dependent mechanism. Neither leucine nor rapamycin had any effect on the activation of the upstream mTORC1 regulators, AMP-activated protein kinase and protein kinase B, in skeletal muscle or liver. The activation of eIF2a and elongation factor 2 was not affected by leucine or rapamycin, indicating that these two pathways are not limiting steps of leucine-induced protein synthesis. These results suggest that leucine stimulates muscle protein synthesis in neonatal pigs by inducing the activation of mTORC1 and its downstream pathway leading to mRNA translation.  相似文献   

Application of biotechnological tools to Quercus improvement     

Ana M. Vieitez  Elena Corredoira  M. Teresa Martínez  M. Carmen San-José  Conchi Sánchez  Silvia Valladares  Nieves Vidal  Antonio Ballester 《European Journal of Forest Research》2012,131(3):519-539

The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.  相似文献   

Effect of Maternal Antibodies on the Pathogenesis of Avian Reovirus Infections in Broiler Chickens Using Real-Time Reverse Transcriptase Polymerase Chain Reaction     

Kejun Guo  Teresa Dormitorio  Shan-Chi Ou  Joseph Giambrone 《农业科学与技术》2012,(9):1058-1063

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Sectioning remote imagery for characterization of <Emphasis Type="Italic">Avena sterilis</Emphasis> infestations. Part B: Efficiency and economics of control     

David Gómez-Candón  Francisca López-Granados  Juan J. Caballero-Novella  Alfonso García-Ferrer  José M. Peña-Barragán  Montserrat Jurado-Expósito  Luis García-Torres 《Precision Agriculture》2012,13(3):337-350

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