Distribution of prion protein genotypes in breeds of sheep in New Zealand     

MA Lee  TR Manley  BC Glass  RM Anderson  SJ Wilson  JS O'Keefe 《New Zealand veterinary journal》2013,61(5):222-227

AIM: To use an established high through-put genotyping procedure to gain an estimate of the frequency of alleles of the prion protein (PrP) gene in some common sheep breeds in New Zealand.

METHODS: Using a genotyping procedure based on matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF), DNA samples from 3,024 sheep from New Zealand, including breeds such as Romney, Texel, Coopworth, Merino and mixedbreed, were isolated, genotyped and the results analysed.

RESULTS: The 15 scrapie genotypes commonly reported, and derived from the five commonly reported allelic variants (ARR, ARQ, AHQ, ARH and VRQ), were all observed in the samples analysed. The estimates were indicative of the frequencies in the population of alleles present in breeds of sheep in New Zealand. There was a significant difference between the frequencies of alleles between breeds, but the ARQ, followed by the ARR allele, were, except in Carwell sheep, the most common alleles present.

CONCLUSION: This study gave an indication of the percentages of PrP gene alleles in sheep in New Zealand, including data previously unreported from breeds in this country. It is of interest because of the relatively large size of the sheep population in New Zealand compared with many countries, and it provides some useful information on the genetic susceptibility or resistance of the sheep population in New Zealand to scrapie. The frequencies of the alleles can be different for an individual breed compared between countries.  相似文献   

Economic analysis of overwinter feeding regimens for channel catfish (Ictalurus punctatus)     

Upton Hatch  Terrill R. Hanson  Myung Kon Kim  Richard T. Lovell 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):141-150

Abstract

Overwintering is a major source of uncertainty for US catfish farmers, particularly decisions on feeding. To address this issue, economic analysis was undertaken using results from pond experiments. Three overwintering strategies (full‐feed, partial‐feed, and no‐feed) for two year classes of catfish were compared. Year 1 fin‐gerlings were 22 g and year 2 fish were 420 g at stocking. Winter feeding was based on temperature and body weight percentages; (1) full‐feeding followed the regime during the November to April period; (2) partial‐fed treatments followed the temperature/body weight regimen only during the months of November, March and April, with no feeding during the coldest months of December to February; and (3) no‐feeding treatments received no feed during the overwinter period. Cost and returns were estimated for each alternative and each alternative was assessed using: (1) overwinter period experimental results, (2) overwinter period experimental results extended through the grow‐out period, (3) overwinter period experimental results extended to minimally acceptable fish sizes, and (4) adjusted estimates to reflect commercial‐scale practices. Sensitivity analyses on feed conversion ratios, stocking rates and fish selling prices were also conducted on commercial‐scale enterprises. Results consistently showed partial‐fed overwinter practices to be most profitable. Secondly, year 1 fish always had higher returns than year 2 fish due largely to the additional overwintering period and associated variable and fixed costs incurred by the year 2 fish production practices. The data upon which this analysis was based do not include the possibility of having a harvest‐sized fish going into the winter period.  相似文献   

A Comparison of Three Conventional Horse Feeders with the Pre-Vent Feeder     

Mark J. CarterTed H. Friend  MSc  PhD  Josie CoverdaleShannon M. Garey  MSc  Amber L. AdamsCooper L. Terrill  BS 《Journal of Equine Veterinary Science》2012

This study compared a feeder with molded cups on the bottom (Pre-Vent feeder) with a commonly used rubber tub and hanging bucket feeder to determine differences in time spent eating and feed wastage. Nine Quarter Horse geldings were fed a 12% crude protein pellet diet at 0.75% body weight twice daily from one of the three feeders twice daily for 3 days, and then switched to the next feeder, following a 3 × 3 replicated Latin square design (n = 9). The horses spent more time eating from the cup feeders (31.15 ± 1.43 minutes) than from bucket (19.39 ± 0.42 minutes) and tub (18.87 ± 0.49 minutes, P < .0001) feeders. When fed from cup feeder, horses dropped significantly less feed (3.2 ± 0.52%) of their ration than when fed from bucket (10.2 ± 1.75%) and from tub (7.0 ± 1.32%, P = .001) feeders. When the most wasteful horse was fed from the cup feeder, he lost a mean of 8.7% of his ration, compared with 32.8% when fed from the bucket, and 26.2% when fed from the tub feeders. The cup design is useful for increasing time spent eating and reducing feed wastage.  相似文献   

Retrospective study of Old World screwworm fly (Chrysomya bezziana) myiasis in 59 dogs in Hong Kong over a one year period     

JC MCNAE  SJ LEWIS 《Australian veterinary journal》2004,82(4):211-214

This retrospective study examined the case records of Old World screw-worm fly myiasis in dogs in Hong Kong's New Territories. Myiasis due to OWSWF was identified and treated in 59 dogs at our clinic over a period of 1 year. Signalment, causes of initial wound, and treatments implemented, are reported. All cases recovered from myiasis with a combination of larvae removal and wound debridement, antibiotics, and topical and systemic insecticides. Whilst this parasite is currently exotic to Australia, much of the country is environmentally suitable (tropical and subtropical) for establishment of OWSWF.  相似文献   

Effects of Gonadotrophin Releasing Hormone Administration on the Pituitary-Ovarian Axis in Anoestrous vs Ovariectomized Bitches     

JJCWM Buijtels  NJ Beijerink  HS Kooistra  SJ Dieleman  AC Okkens 《Reproduction in domestic animals》2006,41(6):555-561

The aim of this study was to determine the effects of gonadotrophin releasing hormone (GnRH) administration on the plasma concentrations of reproductive hormones in intact and ovariectomized (OVX) bitches. Therefore, blood samples were collected at multiple times before and after the administration of 10 microg/kg GnRH (Fertagyl)) for the determination of the plasma concentrations of luteinizing hormone (LH), oestradiol, progesterone and testosterone in six anoestrus and in six OVX bitches. The mean plasma LH concentrations before and 60 min after GnRH administration were significantly lower in the anoestrous bitches than in the OVX bitches. In both groups GnRH administration resulted in a significant increase in the plasma LH concentration. The highest plasma LH concentrations were found at 10 min after GnRH administration and these values did not differ significantly between the two groups. Only in the anoestrous bitches a significant increase in plasma oestradiol concentrations was found after GnRH administration and these values were significantly higher than those in the OVX bitches. The plasma concentrations of progesterone and testosterone were low (close to or below the limit of quantitation) both before and after GnRH administration and the differences between anoestrous and OVX bitches were not significant. It can be concluded that (i) basal plasma LH concentration is significantly higher in OVX bitches than in anoestrous bitches, (ii) plasma LH concentration increases after GnRH administration in both anoestrous and OVX bitches, (iii) GnRH administration causes a significant rise in plasma oestradiol concentration only if ovarian tissue is present and (iv) measurement of plasma progesterone and testosterone concentrations before and after GnRH administration does not aid in distinguishing between anoestrous and OVX bitches. The results of this study may provide a basis for the diagnosis of remnant ovarian tissue and verification of neuter status in the bitch.  相似文献   

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos     

Louise Katherine BARTOLAC  Jenna Louise LOWE  George KOUSTAS  Cecilia SJ?BLOM  Christopher Gerald GRUPEN 《The Journal of reproduction and development》2015,61(6):525-531

The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoop^TM system, or vitrified in a closed device using either the CryoTip^TM or Cryo Bio^TM’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoop^TM: 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures.  相似文献   

Accuracy of the FAMACHA system for on-farm use by sheep and goat producers in the southeastern United States     

Burke JM  Kaplan RM  Miller JE  Terrill TH  Getz WR  Mobini S  Valencia E  Williams MJ  Williamson LH  Vatta AF 《Veterinary parasitology》2007,147(1-2):89-95

FAMACHA is a practical on-farm system designed to provide small ruminant producers a tool for improving their management of Haemonchus contortus infections. Although this system has become very popular and widely accepted by small ruminant producers in many regions of the southern United States, there is very limited data reported on the effectiveness of the FAMACHA system when performed by farmers. The objective of this study was to evaluate the accuracy of the FAMACHA system for on-farm use by small ruminant producers during the summer season. Small ruminant producers from Georgia, Louisiana, Florida, and Puerto Rico were trained to use the FAMACHA system by veterinarians and scientists experienced with this method. FAMACHA scores were assigned at least every 2 weeks by producers to weaned and mature sheep (n=552) and goats (n=676) of various breeds and ages between April and September 2004. At intervals that varied among farms from 2 to 8 weeks, researchers determined body condition scores (BCS; 1=thin and 5=fat) and collected blood and feces from a group of animals selected randomly to determine packed cell volume (PCV) and fecal egg counts (FEC). Two separate anemia thresholds were evaluated; these were defined by either FAMACHA score (>or=3 versus >or=4) or PCV (or=3 were considered anemic and PCV cutoff was or=3 were considered anemic and PCV cutoff was 相似文献   

The structure and regulation of the Irish equine industries: Links to considerations of equine welfare     

Collins J  Hanlon A  More S  Duggan V 《Irish veterinary journal》2008,61(11):746-756

The equine industries in Ireland are vibrant and growing. They are broadly classified into two sectors: Thoroughbred racing, and sports and leisure. This paper describes these sectors in terms of governance, education and training in equine welfare, and available data concerning horse numbers, identification, traceability and disposal. Animal welfare, and specifically equine welfare, has received increasing attention internationally. There is general acceptance of concepts such as animal needs and persons' responsibilities toward animals in their care, as expressed in the 'Five Freedoms'. As yet, little has been published on standards of equine welfare pertaining to Ireland, or on measures to address welfare issues here. This paper highlights the central role of horse identification and legal registration of ownership to safeguard the health and welfare of horses.  相似文献   

Trends in cow numbers and culling rate in the Irish cattle population, 2003 to 2006     

Maher P  Good M  More S 《Irish veterinary journal》2008,61(7):455-463

Cows are the main economic production units of Ireland's cattle industry. Therefore, demographic information, including overall numbers and survival rates, are relevant to the Irish agricultural industry. However, few data are available on the demographics of cows within a national population, either in Ireland or elsewhere, despite the recent development of comprehensive national cattle databases in many EU Member States. This study has sought: to determine the rate of cow culling from the national herd; to determine the rate of culling by type (dairy, beef), age, method of exit, date of exit and interval between last calving and exit; to calculate the national cow on-farm mortality rate; and to compare the Irish rates with published data from other countries. This work was conducted using data recorded in the national Cattle Movement Monitoring System (CMMS). Culling refers to the exit of cows from the national herd, as a result of death but regardless of reason, and cow-culling rate was calculated as the number of cow exits (as defined above) each year divided by the number of calf births in the same year. Culling rate was determined by type (dairy or beef), date of birth, method of exit (slaughter or on-farm death), month of exit and interval between last calving and exit. The average cow-culling rate during 2003 to 2006 was 19.6% (21.3% for dairy, 18% for beef). While comparisons must be treated with caution, it concluded that the overall rates of culling in Ireland fell within published internationally accepted norms. The on-farm mortality rate of 3.2-4.1% was similar to that reported in comparable studies.  相似文献   

Tuberculosis in alpaca (Lama pacos) on a farm in Ireland. 2. Results of an epidemiological investigation     

Connolly Dj  Dwyer P  Fagan J  Hayes M  Ryan E  Costello E  Kilroy A  More S 《Irish veterinary journal》2008,61(8):533-537

Tuberculosis (TB), due to infection with Mycobacterium bovis was diagnosed in a flock of alpaca in Ireland in 2004. An epidemiological investigation was conducted to identify the risk of TB for farmed alpaca where TB is endemic, the origin of the infection, the potential for alpaca-to-alpaca transmission and appropriate control measures. The investigation focused on the alpaca flock (including the farm, animal movements and breeding, feeding and flock health practice), the disease episode (including animal disease events and subsequent control measures) and TB infection risk in the locality. The TB risk to alpaca is high in areas where infection is endemic in cattle and badgers and where biosecurity is inadequate. It is most likely that the source of infection for the alpaca was a local strain of M. bovis, present in cattle in this area since at least 2001. Genotyping of isolates identified a single variable number tandem repeat (VNTR) profile in both cattle and alpaca in this region. Although a tuberculous badger was also removed from the vicinity, bacterial isolation was not attempted. On this farm, infection in alpaca was probably derived from a common source. Alpaca-to-alpaca transmission seems unlikely. Two broad control strategies were implemented, aimed at the rapid removal of infected (and potentially infectious) animals and the implementation of measures to limit transmission. Tests that proved useful in detecting potentially-infected animals included measurement of the albumin-to-globulin ratio and regular body condition scoring. Skin testing was time consuming and unproductive, and early detection of infected animals remains a challenge. The flock was managed as a series of separate groupings, based on perceived infection risk. No further TB cases have been detected.  相似文献