排序方式： 共有45条查询结果，搜索用时 15 毫秒
21.
Gentoku Nakase Yoshizumi Nakagawa Shigeru Miyashita Toshiro Nasu Shigeharu Senoo Hiroko Matsubara Mitsuru Eguchi 《Fisheries Science》2007,73(4):784-791
Bacterial community structures were analyzed in water used for rearing fish larvae by fluorescence in situ hybridization. In Experiment 1, red sea bream Pagrus major larvae were reared in two commercial seed production tanks. The survival rate in Tank 1 was higher than in Tank 2, even though
phytoplankton, Nannochloropsis sp., was added to both tanks. In Tank 2, γ-proteobacteria became dominant (∼70% of total bacteria) on day 13, there after
heavy larval mortalities occurred. In Tank 1, however, α-proteobacteria and the Cytophaga-Flavobacterium cluster were predominant from day − 1 until day 13; no significant mortality was recorded. In Experiment 2, marble goby Oxyeleotris marmoratus larvae were cultured with or without Nannochloropsis sp. At the end of the experiment, larval survival rates in aquaria with Nannochloropsis sp. were significantly (P <0.05) higher than those without. In rearing water without Nannochloropsis sp., γ-proteobacteria increased during rearing. In rearing water with Nannochloropsis sp., α-prote obacteria and the Cytophaga-Flavobacterium cluster were predominant at the beginning of the experiments and the relative abundance of γ-proteobacteria was maintained
at a lower level throughout the experiments. The predominance of α-proteobacteria and the Cytophaga-Flavobacterium cluster appears to be a good indicator of successful larval production. 相似文献
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23.
M Nakai T Nasu 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(4):677-681
Junctional complexes of the epithelia lining the rete testis, efferent ductules, connecting ductules and epididymal duct in the fowl were examined by transmission electron microscopy and by a tracer method using lanthanum nitrate. The junctional complexes were composed of tight junctions, adhering junctions and desmosomes. In the rete testis, one or two points of membrane fusion were observed at the tight junctions. In the efferent and connecting ductules and epididymal duct, the tight junctions consisted of a series of punctate membrane fusions. The adhering junctions and desmosomes showed no remarkable structural differences among these excurrent ducts. Vascularly infused lanthanum nitrate penetrated into the tight junctions of individual epithelia for variable distances, but was prevented from entering the lumen at the site of membrane fusion. These results suggest that the tight junctions can restrict the diffusion of materials via the paracellular route, and that they play an important role in maintaining a suitable fluid environment within the excurrent ducts. 相似文献
24.
Wulansari R Wijaya A Ano H Horii Y Nasu T Yamane S Makimura S 《Journal of the American Animal Hospital Association》2003,39(6):558-562
This report examines the effectiveness of clindamycin for the treatment of babesiosis in dogs (n=10) experimentally infected with Babesia gibsoni (B. gibsoni). Clindamycin (25 mg/kg body weight, per os, q 12 hours for 14 days) gradually reduced parasitemia levels and induced morphological changes that indicated degeneration of parasites (e.g., segmentation; size reduction; localization in the cell limbic and/or torn state of the nucleus; and swelling, decrease, or disappearance of the cytoplasm) in the majority of dogs. Clindamycin treatment reduced the clinical symptoms characteristic of Babesia infection, including anemia, anorexia, and listlessness. Clindamycin might be useful as a medicine for treatment of B. gibsoni infection. 相似文献
25.
Edamura K Itakura S Nasu K Iwami Y Ogawa H Sasaki N Ohgawara H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):549-556
Xenotransplantation of porcine pancreatic endocrine (PE) cells in a diffusion chamber, a bioartificial endocrine pancreas (Bio-AEP), was conducted to total pancreatectomized dogs. Six pancreatectomized dogs were divided into two groups of 3 dogs each. In three dogs of the control group, exogenous insulin was administered twice a day for 30 weeks to maintain fasting blood glucose (FBG) levels within the normal range. The remaining three dogs were implanted with Bio-AEPs (implantation group), in addition to daily insulin administration. In the implantation group, Bio-AEPs containing 1.3 to 1.8 x 10(7) cells per kg of body weight of the recipient were implanted without fixation into the abdominal cavity. In the control group, exogenous insulin requirements did not decrease during the experimental period, whereas it significantly decreased for a certain period (3, 11, 17 weeks) after implantation in all implanted dogs. In the implantation group, laparotomy was performed after FBG and the exogenous insulin requirement increased again and Bio-AEPs were removed. Two Bio-AEPs were completely destroyed, and the remaining one was encapsulated by thin fibrous tissue. In this dog, effusion was present within the capsule, but the Bio-AEP was not destroyed. Histopathologically, the necrosis, presumably caused by hypoxia, of the PE-cells was observed on transmission electron microscopy. In conclusion, Bio-AEP could function for a certain period after implantation in this study. However, more preclinical researches should be needed to apply this technique for the treatment of diabetic dogs. 相似文献
26.
Akira Kawaguchi Hiroyuki Sawada Kouji Inoue Hideo Nasu 《Journal of General Plant Pathology》2005,71(1):54-59
A biovar 3-specific primer set Ab3-F3/Ab3-R4 was designed based on the comparison of sequences of the 16S rDNA region of agrobacteria and related rhizobia for rapid identification of Agrobacterium biovar 3 strains. A 570-bp 16S rDNA fragment was amplified from cell lysates of Agrobacterium biovar 3 strains by polymerase chain reaction (PCR) using Ab3-F3/Ab3-R4 primers. Discrimination of Agrobacterium tumefaciens biovar 3 from Agrobacterium radiobacter biovar 3 and of Agrobacterium biovar 3 strains from other Agrobacterium strains was done simultaneously using multiplex PCR with a mixture of two primer sets (Ab3-F3/Ab3-R4 and VCF3/VCR3) previously designed for the virC region of Ti-plasmid and Ri-plasmid. 相似文献
27.
Sato K Torihashi S Hori M Nasu T Ozaki H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(10):1053-1060
Intestinal muscularis resident macrophages distributed in myenteric region may play an important role in the immunological host defense against infection. In this study, we investigated the phagocytic stimulation of resident macrophages on cyclooxygenase-2 (COX-2) expression and smooth muscle contraction in the small intestine of rat. After the injection of FITC-dextran to rat, phagocytosed macrophages could be detected in the myenteric plexus. FITC-positive macrophages were also immunostained with COX-2 antibody. The number of COX-2 immunopositive cells increased in a time-dependent manner reaching its maximum at 4 hr after the injection, which then decreased gradually but considerable number of cells were still remained on 7 days. The injection of FITC-dextran, however, did not change the population of ED2-positive resident macrophages even on 7 days. Production of PGE2 was significantly higher in the dextran treated tissue as compared to control tissue. In the smooth muscle tissue phagocytosed dextran, carbachol-induced contraction was significantly decreased. The suppression of the carbachol-induced contraction was completely restored by COX inhibitor, indomethacin. Finally we demonstrated that, in freshly isolated macrophage cells, addition of dextran induced a slow and sustained increase in intracellular Ca2+ concentration. These results indicate that phagocytotic activation of muscularis resident macrophages induces COX-2 gene expression and then results in production of PGE2 to suppress the smooth muscle contractile activity. 相似文献
28.
Satoshi Taba Ayano Takara Kanami Nasu Nao Miyahira Tetsuya Takushi Zen-ichi Moromizato 《Journal of General Plant Pathology》2009,75(2):160-162
An outbreak of black mottle and dieback on basil (Ocimum basilicum L.; Lamiaceae) was recorded in a greenhouse in Okinawa Prefecture, Japan during 2004. The causal agent was identified as
Alternaria alternata (Fr.) Keissler based on morphological characters and growth temperature. This report is the first of Alternaria leaf spot
of basil caused by A. alternata. 相似文献
29.
F. de la S. Sabate Y. Nakagawa T. Nasu W. Sakamoto S. Miyashita 《Aquaculture International》2013,21(1):177-181
Tank wall collision is one of the major causes of mortality during the early-stage rearing of Pacific bluefin tuna, Thunnus orientalis (PBT). Therefore, to design a rearing environment that meets the needs of juvenile PBT, it is important to gather information about their swimming capabilities. We conducted experiments to examine the relative critical swimming speed (RCSS) and maximum sustainable swimming speed (MSSS) of early-stage PBT. The fish were kept in 3-tonne tanks and fed on artificial pellets every 2 h from dusk to dawn. We conducted two sets of experiments to measure swimming speed; the fish were introduced one at a time into a water funnel, and the water current velocity was gradually increased over time to estimate RCSS, or the water current was kept at a constant velocity to estimate MSSS. We measured the RCSS of 72 PBT juveniles (24–29 days after hatching (DAH); standard length (SL), 15.0 ± 2.3 mm) and the MSSS of 32 PBT juveniles (28–37 DAH; SL, 20.0 ± 5.1 mm) in the laboratory. The RCSS ranged from 4.7 to 20.3 SL/s (average, 12.4 ± 3.3 SL/s), and the MSSS was estimated to be approximately 4 SL/s. We speculate that introducing a water current in the rearing tank of no more than 4 SL/s could positively affect the survival of juvenile PBT. 相似文献
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