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101.
102.
Kazuko OGATA Aiko SASAKI Yuka KATO Arisa TAKEDA Mikio WAKABAYASHI Borjigin SARENTONGLAGA Mio YAMAGUCHI Asuka HARA Rika FUKUMORI Yoshikazu NAGAO 《The Journal of reproduction and development》2015,61(2):116-122
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality of frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in extender (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics and 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. The samples were cooled to 4 C and then frozen in liquid nitrogen vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM GSH group than in the control or 2.5 and 10 mM GSH groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM GSH group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the GSH-treated groups (5 and 10 mM) compared with the control. Lipid
peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM GSH groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM GSH group was used for transcervical insemination of 4 bitches, resulting in delivery of 5 puppies from 2 bitches. These results indicate that supplementation of semen extender with 5 mM GSH was effective in improving motility, longevity and acrosomal integrity and inhibiting LP levels in post-thaw canine spermatozoa, without any adverse impacts on full-term development after transcervical insemination. 相似文献
103.
Yuko TAJIMA Kyoko SASAKI Nobuyuki KASHIWAGI Tadasu K. YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):989-992
Lobomycosis is a chronic fungal disease caused by the etiologic agent, Lacazia loboi, in the skin and subcutaneous tissues in humans and dolphins in tropical and transitional tropical climates. An Indo-Pacific bottlenose dolphin (Tursiops aduncus) stranded in Kagoshima, Japan, had severe skin lesions characterized by granulomatous reactions and hyperkeratosis that were similar to those of the lobomycosis, but no fungal organism was observed in the skin lesion. In this paper, we report a stranded Indo-Pacific bottlenose dolphin with lobomycosis-like lesions based on pathological examinations in Japan. 相似文献
104.
Masanao MATAYOSHI Takashi KITANO Tetsu SASAKI Masaji NAKAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):705-710
A total of 349 Salmonella enterica subspecies enterica
serovar Choleraesuis (S. Choleraesuis) strains, which were isolated
between 2008 and 2012 from 349 pigs at two slaughterhouses in Okinawa Prefecture, Japan,
were investigated for antimicrobial susceptibility and the presence of antimicrobial
resistance genes. All isolates were resistant to at least four antimicrobial agents. The
antimicrobial agents for which isolates showed a high incidence of resistance were as
follows: ampicillin (100%) and streptomycin (100%), followed by gentamicin (99.7%),
oxytetracycline (99.7%), sulfamethoxazole/trimethoprim (99.4%), nalidixic acid (40.1%) and
oxolinic acid (40.1%). All isolates were sensitive to cefuroxime, ceftiofur, colistin,
fosfomycin, enrofloxacin, orbifloxacin and danofloxacin. The predominant resistance
phenotypes and genotypes were: resistance to ampicillin, streptomycin, gentamicin,
oxytetracycline and sulfamethoxazole/trimethoprim (58.5%, 204/349) and
blaTEM-strA-strB-aadA1-aadA2-aacC2-tet
(B)-sul1-sul2-dhfrXII-dhfrXIII (36.1%, 126/349). The quinolone
resistance-determining regions (QRDRs) of gyrA, gyrB, parC and
parE of the quinolone-resistant isolates (n=12) showed amino acid
substitutions of Ser-83→Phe or Asp-87→Tyr in GyrA and Ser-107→Ala in ParC. To our
knowledge, this is the first report on the molecular characterization of antimicrobial
resistance among S. Choleraesuis strains in Japan. 相似文献
105.
Abdul Razaq IRSHAD Taihei SASAKI Tomoaki KUBO Naoyuki ODASHIMA Keiji KATANO Takeshi OSAWA Toru TAKAHASHI Yoshiaki IZAIKE 《The Journal of reproduction and development》2015,61(5):485-488
The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3– < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively). 相似文献
106.
107.
Chih Chun WU Makoto NAKATA James Kenn CHAMBERS Kazuyuki UCHIDA Nobuo SASAKI Yasutsugu MIWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):685
A retrospective study involving eight African pygmy hedgehogs histopathologically diagnosed with granulosa cell tumors was conducted. The age at onset was 2.2–4.5 years, with a median age of 3.6 years. The most common clinical signs were hematuria and abdominal distension, which were observed in >50% cases. Exploratory laparotomy was performed in all cases, and ovariohysterectomy or excision of the abdominal mass was performed. Patients with only hematuria survived for >250 days after surgery, whereas those with initial ascites showed recurrence of ascites or tumor growth and survived for approximately 130 days after surgery. Intraperitoneal injection of carboplatin was performed in three recurrent cases. In one of these three cases, the tumor mass disappeared. Hence, carboplatin can be considered a potential antineoplastic drug for the treatment of granulosa cell tumors. 相似文献
108.
Takeshi SEKIGAWA Toyomi TAKAHASHI Tetsuya TAKATSU Syuichi NISHIUCHI Masayoshi SASAKI Fumiyasu SHIOKAWA 《Fisheries Science》2003,69(3):575-580
ABSTRACT: Age and growth of Sebastes vulpes collected from the coastal waters of western Hokkaido were studied. Observation of the otolith margin verified that annuli (outer margins of the opaque zone) were produced chiefly from July to August. This period was associated with parturition and the birth season. The maximum age estimated by the surface method was 12 years but the oldest fish was aged at 35 years by the cross-section method. The surface method was inadequate for aging of S. vulpes older than 6 years because of the underestimation of age. No significant difference was found in the parameters of the growth equations between both sexes. The von Bertalanffy growth curve combined for both sexes was as follows: SL t = 358.6(1 − exp−0.156( t +0.820) ), where SL t is standard length (mm) at age t (after parturition in years). It seems likely that S. vulpes grows slowly and lives longer than previously thought. 相似文献
109.
ABSTRACT: Baited traps were used over 24 h to reveal the diel activity and vertical distribution of three dominant lysianassoid amphipods ( Scopelocheirus onagawae , Anonyx omorii , Anonyx abei ) in Onagawa Bay, north-eastern Japan. The three species were the most dominant small crustacean scavengers in the bay. Most individuals were caught by traps deployed 0.3 m above the bottom, showing that they were active in the layers close to the sea bottom. No habitat segregation was found among species or developmental stages, suggesting that these scavengers rely on the food supply from bottom deposits. The three species increased remarkably in numbers of collected individuals at night, showing a rise in feeding activity. The two Anonyx species were collected only during the night, and S. onagawae was collected mostly during the night. The nocturnal activity appears to be an adaptation to reduce predation risks. However, juveniles of S. onagawae appeared even during the day, suggesting that feeding might take precedence over avoiding predation. Baited traps collected ovigerous females of S. onagawae but not of Anonyx species; the reason for the difference could not be determined. 相似文献
110.
Takeshi Takasaki Kazuma Okada Carlos Castillo Yuki Moriya Toshihiro Saito Yutaka Sawamura Naoko Norioka Shigemi Norioka Tetsu Nakanishi 《Euphytica》2004,135(2):157-167
A new S
9-allele was discovered in 6 Japanese pear cultivars, ‘Shinkou’, ‘Shinsei’, ‘Niitaka’, ‘Amanogawa’, ‘Nangetsu’ and ‘Nansui’.
cDNA encoding S
9-RNase, a stylar product of S
9-allele, was cloned from pistils of ‘Shinkou’ and ‘Shinsei’ by 3' and 5' RACE. The S
9-RNase gene had an open reading frame of 684 nucleotides encoding 228 amino acid residues. S
9-RNase had a hypervariable (HV) region different from S
1- to S
8-RNase and shared higher similarity (95.2%) with apple S
3-RNase than with 8 Japanese pear S-RNases (from 61.0% to 70.7%). Genomic PCR with primers ‘FTQQYQ’ and ‘anti-(I/T) IWPNV’ provided S
1- to S
9-amplicon (product), but could not discriminate the S
2 from the S
9 of ca. 1.3 kb. The S
2 and S
9 were distinguished by digestion with AflII and BstBI, respectively. The digestion with nine S-allele-specific restriction endonucleases, SfcI, AflII, PpuMI, NdeI,AlwNI, HincII, AccII, NruI and BstBI, distinguished S
1 to S
9, establishing that this PCR-RFLP system is useful for S-genotype assignments in Japanese pear harboring S
1- to S
9-allele. ‘Shinkou’, ‘Shinsei’, ‘Nangetsu’ and ‘Nansui’ assigned as S
4
S
9 were determined to be cross incompatible.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献