Soil selenium fractionation,depth profiles and time trends in a vegetated site at Kesterson Reservoir     

Tokunaga  Tetsu K.  Lipton  Douglas S.  Benson  Sally M.  Yee  Andy W.  Oldfather  Joan M.  Duckart  Eugene C.  Johannis  Paul W.  Halvorsen  Kathleen E. 《Water, air, and soil pollution》1991,(1):31-41

Kesterson Reservoir, in the San Joaquin Valley of California, was used for disposal of saline, seleniferous agricultural drain waters during the years 1981 - 1986. The combined effects of infrequent drain water application, reduction and oxidation of Se, and evapotranspirative removal of soil water within the upper 1 m of the soil profiles appear to provide explanations for distribution patterns of Se in soils of upland sites. From 1987 to 1990, an approximate doubling in average soil solution Se concentrations was observed. It is shown that currently only relatively insignificant quantities of Se have been transported into the Pond 11 soils from the shallow water table. Sequential extraction of these upland soils indicated that substantial inventories of Se were associated with soluble, adsorbed, carbonate, and soil organic matter fractions, while Se retained within refractory inorganic fractions were minor. The mobilization of Se from these potentially labile pools may account for the observed increases in soil solution concentrations of Se following the draining of Kesterson Reservoir.  相似文献   

Contribution of iron associated with high-molecular-weight substances to the maintenance of the SPAD value of young leaves of barley under iron-deficient conditions     

Midori HIRAI  Kyoko HIGUCHI  Hiroshi SASAKI  Tomoko SUZUKI  Teppei MARUYAMA  Masaaki YOSHIBA  Toshiaki TADANO 《Soil Science and Plant Nutrition》2007,53(5):612-620

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Prognostic value of small intestinal dilatation in dogs with protein-losing enteropathy     

Hiroshi OHTA  Noriyuki NAGATA  Nozomu YOKOYAMA  Tatsuyuki OSUGA  Noboru SASAKI  Keitaro MORISHITA  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):378

To date, little is known about the prognostic significance of ultrasonographic findings in dogs with protein-losing enteropathy (PLE). The aim of this retrospective study was to examine the prognostic value of ultrasonographic findings in dogs with PLE. A total of 26 dogs with PLE were included: 20 dogs with chronic enteropathy and 6 dogs with gastrointestinal lymphoma. The presence of small intestinal dilatation was associated with shorter survival time in dogs with PLE (P=0.003). The presence of hyperechoic intestinal mucosal striations was associated with longer survival time in dogs with PLE (P=0.0085). The results of the current study indicate that the presence of small intestinal dilatation might be associated with poor prognosis in dogs with PLE.  相似文献   

ECG and heart rate determination in fetal cattle using a digital signal processing method     

Wenxi CHEN  Tetsu NEMOTO  Toshio KOBAYASHI  Toshiyuki SAITO  Etsuko KASUYA  Yoshifumi HONDA 《Animal Science Journal》2002,73(6):545-551

A method of recording the fetal electrocardiogram (ECG) and heart rate (HR) is described. An abdominal lead and a thoracic lead were used to measure the fetal ECG and maternal ECG, respectively. The maternal component in the abdominal lead measurement was estimated by a digital adaptive filter using the thoracic lead measurement as a reference. By suppressing the estimated maternal components in the abdominal lead, the fetal ECG could be detected. The beat‐by‐beat fetal QRS complex peaks were determined by a digital matched filter from the fetal ECG enhanced in this way. The method was trialed in 10 pregnant Holstein cows at gestational periods ranging from 136 to 224 days. The results show that this method can extract the fetal ECG and determine the fetal HR from the raw noisy measurement data. The results suggest that the method would have applicability in monitoring fetal ECG as well as providing a non‐invasive, continuous HR profile during gestation, which will enable better understanding of the development of cattle fetuses before delivery.  相似文献   

Effects of FK506 and rapamycin on formation of the neural tube in chick embryos     

Koji OBATA  Masafumi KOIDE  Kohzo NAGATA  Akio IIO  Shigenobu YAZAWA  Tamao ONO  Shin-ichi SASAKI  Yoshiji YAMADA  Rocky S. TUAN  Mitsuhiro YOKOTA 《Animal Science Journal》2002,73(3):229-234

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母马生长激素细胞和催乳激素细胞的形态计测学研究     

谭建华  安铁洙  岳玉环  万忠海  李阳芳  佐佐木文彦 《动物医学进展》2001,22(3):68-70

利用免疫组织化学和形态计测学的方法 ,观察了 1 8匹成熟雌性蒙古马的脑垂体前叶生长激素细胞和催乳激素细胞的数量和面积 ,同时利用放射免疫分析方法检测了这两种激素的血浆水平。结果表明 ,每个马脑垂体前叶中 ,生长激素细胞的平均数量为 6 .42× 1 8,每个细胞的平均面积为 82 .40μm2 ;催乳激素细胞的平均数量为 6 .0 7× 1 0 8,每个细胞的平均面积为 47.31μm2 。生长激素的血浆含量平均为 2 .84ng/ m L,但个体差异较大 ,变异系数高达 78.5 % ,催乳激素的血浆含量平均为 7.2 6 ng/ m L。本研究结果揭示 :母马脑垂体生长激素细胞和催乳激素细胞的数量和面积并不是决定母马这两种激素血中浓度的唯一重要因素 ;生长激素血中浓度上的个体差异 ,可能与其搏动性分泌形式有关  相似文献   

A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle   总被引：1，自引：0，他引：1  

Hidetoshi Higuchi  Hidetomo Iwano  Kazuhiro Kawai  Takehiro Ohta  Tetsu Obayashi  Kazuhiko Hirose  Nobuhiko Ito  Hiroshi Yokota  Yutaka Tamura  Hajime Nagahata 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):191-193

A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 10³ cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms.  相似文献   

Economic effects of using sexed semen in Japanese dairy herds     

Osamu SASAKI  Hirohisa KIMURA  Kazuo ISHII  Masahiro SATOH  Yoshitaka NAGAMINE  Kunio YOKOUCHI 《Animal Science Journal》2011,82(3):486-493

Dairy farm agricultural income should increase when sexed semen is used instead of unsexed semen. This study simulated three scenarios: using unsexed semen (N), using X sperm of Holstein and unsexed semen of Japanese Black (H), and using X sperm of Holstein and Y sperm of Japanese Black (HJ). Two management scenarios were considered: conventional management (CONV); and the use of Japanese Black semen with surplus cows to produce F₁s where sufficient replacement female Holstein calves were obtained for maintenance (MAINT). In CONV, the agricultural incomes of H and HJ were 610 000–2 400 000 yen higher than that of N. The agricultural income increased when the difference in the prices between Holstein males and average F₁ male/female calves was 34 000–50 000 yen, that between Holstein male and female calves was 80 000–110 000 yen, and that between F₁ male and female calves was 50 000–90 000 yen. Therefore, the agricultural income can be increased by using sexed semen to select calves of a more valued breed and sex.  相似文献   

Qualitative and Quantitative Contrast Enhanced Ultrasonography of the Pancreas Using Bolus Injection and Continuous Infusion Methods in Normal Dogs     

Sue Yee LIM  Kensuke NAKAMURA  Keitaro MORISHITA  Noboru SASAKI  Masahiro MURAKAMI  Tatsuyuki OSUGA  Hiroshi OHTA  Masahiro YAMASAKI  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1601-1607

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Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)     

Motoki SASAKI  Yoko AMANO  Daisuke HAYAKAWA  Toshio TSUBOTA  Hajime ISHIKAWA  Toshihiro MOGOE  Seiji OHSUMI  Masafumi TETSUKA  Akio MIYAMOTO  Yutaka FUKUI  Teguh BUDIPITOJO  Nobuo KITAMURA 《The Journal of reproduction and development》2013,59(2):159-167

There are few reports describing the structure and function of the whale placenta with the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of the Antarctic minke whale were observed morphologically and immunohistochemically. Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial placenta. The chorion was interdigitated to the endometrium by primary, secondary and tertiary villi, which contained no specialized trophoblast cells such as binucleate cells, and the interdigitation became complicated with the progress of gestation. Furthermore, fetal and maternal blood vessels indented deeply into the trophoblast cells and endometrial epithelium respectively with fetal growth. The minke whale placenta showed a fold-like shape as opposed to a finger-like shape. In both nonpregnant and pregnant uteri, many uterine glands were distributed. The uterine glands in the superficial layer of the pregnant endometrium had a wide lumen and large epithelial cells as compared with those in the deep layer. On the other hand, in the nonpregnant endometrium, the uterine glands had a narrower lumen and smaller epithelial cells than in the pregnant endometrium. In immunohistochemical detection, immunoreactivity for P450scc was detected in most trophoblast cells, but not in nonpregnant uteri, suggesting that trophoblast epithelial cells synthesized and secreted the sex steroid hormones and/or their precursors to maintain the pregnancy in the Antarctic minke whale.  相似文献