Canine cutaneous epitheliotropic T-cell lymphoma with vesiculobullous lesions resembling human bullous mycosis fungoides     

Petra Bizikova  Keith E. Linder†  Steven E. Suter  Arnaud J. Van Wettere†  Thierry Olivry 《Veterinary dermatology》2009,20(4):281-288

The broad spectrum of clinical signs in canine cutaneous epitheliotropic T-cell lymphoma mimics many inflammatory skin diseases and is a diagnostic challenge. A 13-year-old-male castrated golden retriever crossbred dog presented with multifocal flaccid bullae evolving into deep erosions. A shearing force applied to the skin at the periphery of the erosions caused the epidermis to further slide off the dermis suggesting intraepidermal or subepidermal separation. Systemic signs consisted of profound weight loss and marked respiratory distress. Histologically, the superficial and deep dermis were infiltrated by large, CD3-positive neoplastic lymphocytes and mild epitheliotropism involved the deep epidermis, hair follicle walls and epitrichial sweat glands. There was partial loss of the stratum basale. Bullous lesions consisted of large dermoepidermal and intraepidermal clefts that contained loose accumulations of neutrophils mixed with fewer neoplastic cells in proteinaceous fluid. The lifted epidermis was often devitalized and bordered by hydropic degeneration and partial epidermal collapse. Similar neoplastic lymphocytes formed small masses in the lungs associated with broncho-invasion. Clonal rearrangement analysis of antigen receptor genes in samples from skin and lung lesions using primers specific for canine T-cell receptor gamma (TCRγ) produced a single-sized amplicon of identical sequence, indicating that both lesions resulted from the expansion of the same neoplastic T-cell population. Macroscopic vesiculobullous lesions with devitalization of the lesional epidermis should be included in the broad spectrum of clinical signs presented by canine cutaneous epitheliotropic T-cell lymphoma.  相似文献   

Identification of CD3+ T lymphocytes in the green turtle Chelonia mydas     

Fernando A. Muoz  Sergio Estrada-Parra  Andres Romero-Rojas  Thierry M. Work  Erik Gonzalez-Ballesteros  Iris Estrada-Garcia 《Veterinary immunology and immunopathology》2009,131(3-4):211-217

To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies.  相似文献   

Specific proteins allow classification of pigs according to sire breed, rearing environment and gender     

Anthony Kwasiborski  Thierry Sayd  Christophe Chambon  Vronique Sant-Lhoutellier  Dominique Rocha  Claudia Terlouw 《Livestock Science》2009,122(2-3):119-129

The present study used a proteomic data set obtained from a 2 × 2 × 2 factorial experiment on longissimus muscle of 24 pigs to identify single or couples of proteins allowing correct classification of pigs according to gender, sire breed, or rearing environment. An actin isoform, a myosin light chain 2 isoform and cytochrome Bc1 allowed each, correct classification of all pigs according to gender. Peroxiredoxin 6 allowed correct classification of 23 pigs according to indoor or outdoor rearing environment, but only if gender was also taken into account. Heat shock protein 73 allowed correct classification of 21 pigs according to sire breed, Duroc or Large White. Results show that proteins may be used as biomarkers for traceability or genetic research. Relationships between treatment factors and intracellular regulatory mechanisms associated with these proteins are discussed.  相似文献   

Effect of tapping activity on the dynamics of radial growth of Hevea brasiliensis trees     

Silpi U  Thaler P  Kasemsap P  Lacointe A  Chantuma A  Adam B  Gohet E  Thaniswanyankura S  Améglio T 《Tree physiology》2006,26(12):1579-1587

Rubber tree (Hevea brasiliensis Müll. Arg.) radial growth dynamics were monitored with displacement sensors, together with latex production, to investigate three aspects of the dual production of latex and wood: (1) the usefulness of fine-scale dendrometric measurements as a physiological tool to detect water shortage through radial growth; (2) the dynamic aspects, both at the seasonal and at the multi-year scale, of the competition between latex and wood production; and (3) the spatial distribution of radial growth rates around the tapping cut. Radial growth of untapped control trees started with the onset of the rainy season and lasted until the onset of the dry season, ceasing completely during the driest period. Displacement sensors provided a sensitive means of detecting water shortage, with a clear correlation between diameter variations and changes in water availability (both daily evapotranspiration and monthly rainfall) over the whole annual cycle. However, the correlation was significantly disturbed in tapped trees. After resumption of tapping, the radial growth rate dropped sharply within two weeks and the effect persisted throughout the whole season, so that the cumulative growth of tapped trees was about half that of untapped trees, with the cumulative growth deficit reaching 80% for the period from mid-June to November. This long-known negative impact of tapping on growth was much stronger in the second year of tapping than in the first, whereas latex production increased significantly between the first and second year of tapping. The increased latex production, which could not be ascribed to climatic conditions, shows that the establishment of an artificial latex sink is a progressive, long-term process likely involving many aspects of metabolism. As expected, ethylene significantly increased latex production in both years; however, ethylene had no effect on the growth rates of tapped trees. Radial growth was differentially affected at different locations around the tapping cut, with growth rates significantly lower in the tapped panel than in the untapped panel, and higher above the cut than below the cut. Thus, caution is needed when deriving whole stem wood production from girth measurements at one location on the stem, especially from girth measurements made close to the tapping cut. This also provides new evidence for the location of the latex regeneration area in the tapped panel, below the cut.  相似文献   

“Aberrant” plants in cauliflower: 2. Aneuploidy and global DNA methylation     

Véronique Chable  Alain Rival  Thierry Beulé  Joseph Jahier  Frédérique Eber  Valérie Cadot  François Boulineau  Armel Salmon  Henri Bellis  Maria J. Manzanares-Dauleux 《Euphytica》2009,170(3):275-287

Aberrant phenotypes of cauliflower were detected throughout the cultivation period and in any variety type. The rate of these phenotypes in the field has recently increased. We reported previously on the first part of our results which showed that (1) the rate of aberrant plants varied with genotype and cultivation area, (2) the aberrant phenotypes can evolve or reverse to normality during the plant cycle and (3) the capacity to express a variant phenotype can be transmitted to the progeny. An epigenetic hypothesis has been proposed to explain the determinism of the phenomenon. Further investigation on the “aberrant” character focussed on the flow cytometric estimation of ploidy levels and on the parallel observation of meiosis. Only a fraction of aberrant plants did show aneuploidy and various ploïdy levels were found for the same phenotype. Indeed, aneuploidy could not be related to the aberrant phenotype although it could probably be a consequence of the aberration phenomenon. HPLC analysis of global DNA methylation rates showed that DNA hypermethylation occurred in plants which exhibited an evolution of their phenotype during vegetative cycle. The epigenetic origin of aberrant phenotypes in cauliflower is discussed with reference to epigenetic diseases described in human beings.  相似文献   

Production and isolation of azaspiracid-1 and -2 from Azadinium spinosum culture in pilot scale photobioreactors     

T Jauffrais  J Kilcoyne  V Séchet  C Herrenknecht  P Truquet  F Hervé  JB Bérard  C Nulty  S Taylor  U Tillmann  CO Miles  P Hess 《Marine drugs》2012,10(6):1360-1382

Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell · mL(-1) at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day(-1), with optimum toxin production at 0.25 day(-1). After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures.  相似文献   

Pasteurization Conditions and Evaluation of Quality Parameters of Frozen Packaged Crab Meat     

Jimena Bernadette Dima  Pedro Baron 《Journal Of Aquatic Food Product Technology》2013,22(5):745-759

ABSTRACT

Ovalipes trimaculatus is a crab recognized as a new resource with fishing value to obtain frozen products. Pasteurization conditions of meat crab at temperatures below 85ºC (60, 72, and 82ºC) were established to achieve better quality attributes in the frozen product. The lethality curves of Staphylococcus aureus and Listeria monocytogenes were measured, and the decimal reduction times (D) and Z values were determined. Heat transfer during the pasteurization process of pouches containing crab meat was simulated using a computational code in finite elements, and the mathematical model was experimentally validated. Thermal histories were coupled to the microbial lethality kinetics of the most heat resistant pathogen microorganism in order to establish pasteurization times necessary for the process system design. The predicted pasteurization conditions were microbiologically validated. The pouches were frozen under industrial conditions and stored at ?22ºC for 1 year. The influence of the type of packaging (vacuum and nonvacuum plastic pouches) on physicochemical and sensory quality parameters of frozen crab meat (color, exudate, lipid oxidation, water holding capacity, and overall acceptability) were analyzed observing better performance under vacuum conditions.  相似文献   

Evaluation of thermomechanical pretreatment for enzymatic hydrolysis of pure microcrystalline cellulose and cellulose from Brewers’ spent grain     

Guillaume PierreFrédéric Sannier  Romain GoudeArmelle Nouviaire  Zoulikha Maache-RezzougSid-Ahmed Rezzoug  Thierry Maugard 《Journal of Cereal Science》2011,54(3):305-310

Microcrystalline cellulose (Avicel PH102) and Brewers’ spent grain (BSG) were subjected to Détente Instantanée Contrôlée (DIC) thermomechanical pre-treatment before exposure to cellulases (Celluclast 1.5 L). In a first part, we showed that the addition of β-glucosidase (Novozym-188) increased the hydrolysis yield of Avicel. A maximal theoretical yield (100%), was obtained for 5 and 10 g/L of Avicel using a mixture of Celluclast 1.5 L/Novozym-188. After DIC pre-treatments, the initial rate and final yield of hydrolysis decreased in comparison with those from untreated microcrystalline cellulose. This phenomenon may be due to the modification of the crystallinity of pure cellulose and the formation of inhibitors during the pre-treatment. In a second part, BSG was thermomechanically pre-treated and hydrolyzed. The results showed that the hydrolysis yield of BSG treated at pressure levels between 2 and 7 bar during 15 min was strongly improved compared to hydrolysis yield of untreated BSG. The optimized hydrolysis process, under intensive DIC conditions, achieved a glucose yield corresponding to 100% of the theoretical cellulose value. The morphology of BSG samples was studied with Scanning Electronic Microscopy (SEM) and highlighted that the structure of pre-treated BSG showed an important disruption compared to the rigid structure of untreated BSG.  相似文献   

Electrocardiographic,echocardiographic, and left atrial strain imaging features of a dog with atrial flutter and third-degree atrioventricular block     

M. Baron Toaldo  G. Romito  M. Cipone  A. Diana  M. Tursi 《Journal of Veterinary Cardiology》2017,19(5):462-468

A 14-year-old American Staffordshire terrier was presented for episodes of exercise-induced syncope. At admission, atrial flutter coupled to third-degree atrioventricular block was diagnosed electrocardiographically. On the second day of hospitalization, surface electrocardiogram revealed spontaneous conversion to sinus rhythm with persistence of atrioventricular block. Complete transthoracic echocardiograms were performed after each electrocardiographic examination. The combined use of conventional echocardiography with tissue Doppler imaging-based modalities allowed to investigate the atrial electromechanical correlation and function during typical atrial flutter and after its resolution.  相似文献   

Epidermolysis bullosa simplex in sibling Eurasier dogs is caused by a PLEC non‐sense variant          下载免费PDF全文

Elizabeth A. Mauldin  Ping Wang  Thierry Olivry  Paula S. Henthorn  Margret L. Casal 《Veterinary dermatology》2017,28(1):10-e3

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