Databases tailored for biodiversity conservation     

Smith AT  Boitani L  Bibby C  Brackett D  Corsi F  da Fonseca GA  Gascon C  Dixon MG  Hilton-Taylor C  Mace G  Mittermeier RA  Rabinovich J  Richardson BJ  Rylands A  Stein B  Stuart S  Thomsen J  Wilson C 《Science (New York, N.Y.)》2000,290(5499):2073-2074

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Catch Crop and Animal Slurry in Spring Barley Grown with Straw Incorporation     

Ingrid K. Thomsen 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):166-170

Abstract

Four rates of straw (0, 4, 8 and 12 t ha^?1 yr^?1) were incorporated in a field experiment with continuous spring barley. The experiment was conducted on a sandy soil (5.5% clay) and a sandy loam soil (11.2% clay). After eight years, the straw incorporation was combined with catch-crop growing with and without winter application of animal slurry and also spring fertilization with mineral fertilizer (0, 50, 100 or 125 kg N ha^?1 yr^?1). The combined experiment was conducted for three lyears on the sandy soil and for four years on the sandy loam soil. The effects on barley dry matter yield and N uptake are presented together with the long-term effects of the straw incorporations on crop growth and soil C and N. Grain yield on the sandy loam was unaffected by straw incorporation. On the sandy soil the highest straw application rates reduced grain yield in the unfertilized barley. When the barley received mineral fertilizer at recommended levels (100 kg N ha^?1 yr^?1), grain yield on this soil was also unaffected by the high straw rates. Including a catch crop had a positive effect on the grain yield of barley on both soils. The total N uptake in grain and straw generally increased with straw application up to 8 t ha^?1 yr^?1. With the highest straw application rate (12 t ha^?1 yr^?1), the total N uptake decreased but still exceeded N uptake in barley grown with straw removal. The barley accumulated higher amounts of N when a catch crop was included. The total N uptake in the barley was significantly higher after animal slurry application. The extra N uptake, however, was much lower than the amounts of N applied with the slurry. Incorporation of straw had only a small influence on N uptake after slurry application. The straw, therefore, was not able to store the applied N during winter. In the two four-year periods before the combined experiment, grain yield on the sandy loam was generally negatively affected by straw incorporations. In the second period, N uptake began to show a positive effect of the straw. On the sandy soil, grain yield and N uptake during the whole period were generally positively affected by the straw incorporations except for the highest straw rate (12 t ha^?1 yr^?1). The sandy loam soil showed higher increases in C and N content after the repeated straw incorporations and catch-crop growing than the sandy soil. When application of animal slurry was combined with the catch crop, no further increases in soil C and N were found relative to soil where a catch crop was grown without slurry application. Large amounts of the N applied with the slurry may therefore have been lost by denitrification or nitrate leaching.  相似文献   

Dead or alive — Old empty shells do not prompt false-positive results in environmental DNA surveys targeting the freshwater pearl mussel (Margaritifera margaritifera L.)     

Jes Jessen Rasmussen  Liselotte Wesley Andersen  Trine Just Johnsen  Jens Thaulow  Marc Anglès d'Auriac  Søren Nøhr Thomsen  Martin Hesselsøe 《水产资源保护:海洋与淡水生态系统》2021,31(9):2506-2514

1. Environmental DNA (eDNA) from water samples is increasingly used to detect the presence and distribution of species in aquatic ecosystems. However, before implementing eDNA in monitoring programmes, various species-specific sampling or analytical issues remain to be resolved in order to minimize frequencies of false-positive and -negative results. For example, empty shells from freshwater pearl mussels (Margaritifera margaritifera) contain extractable DNA (chemical extraction from ground-up shells) suggesting a risk of false-positive samples at stream sites with extinct populations but with empty shell material remaining.
2. The aim of this study was to investigate whether empty and naturally degrading shells from M. margaritifera can cause false-positive eDNA signals in water samples.
3. Water samples were collected from outdoor stream channels (in Lemming, Denmark) with living freshwater pearl mussels or empty shell material (density ~10 individuals m⁻²) during a 3-week experimental period. Living freshwater pearl mussels were collected from Hemgravs stream in Sweden and transported to Denmark according to permissions granted by the Swedish and Danish authorities.
4. All water samples from stream channels containing empty shells were negative for eDNA indicating that eDNA traces in stream water are most likely to originate from living individuals located upstream of the sampling site. Water samples collected from stream channels containing living individuals of M. margaritifera were consistently positive for eDNA except for one sample (interpreted as a false negative).
5. The study shows that positive eDNA signals for freshwater pearl mussels most likely reflect the presence of living individuals. Consequently, we suggest that eDNA should be used to locate remaining population fragments of M. margaritifera in deep and turbulent streams, providing a platform for faster and more efficient decision making when launching investigative and mitigation initiatives.

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The role of muscle activation in cruciate disease     

Caroline P. Adrian MSPT  PhD  Kevin K. Haussler DVM  DC  PhD  Diplomate ACVSMR  Raoul F. Reiser II  MA  PhD  CSCS  FACSM  Cheryl Riegger‐Krugh PT  MS  ScD  Ross H. Palmer DVM  Diplomate ACVS  Robert A. Taylor DVM  Diplomate ACVS & ACVSMR 《Veterinary surgery : VS》2013,42(7):765-773

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Nitrogen uptake,nitrate leaching and root development in winter‐grown wheat and fodder radish          下载免费PDF全文

L. J. Munkholm  E. M. Hansen  I. K. Thomsen  E. M. Wahlström  H. S. Østergaard 《Soil Use and Management》2017,33(2):233-242

Early seeding of winter wheat (Triticum aestivum L.) has been proposed as a means to reduce N leaching as an alternative to growing cover crops like fodder radish (Raphanus sativus L.). The objective of this study was to quantify the effect of winter wheat, seeded early and normally, and of fodder radish on N dynamics and root growth. Field experiments were carried out on a humid temperate sandy loam soil. Aboveground biomass and soil inorganic N were determined in late autumn; N uptake and grain yield of winter wheat were measured at harvest. Nitrate leaching was estimated from soil water samples taken at 1 m depth. Root growth was measured late autumn using the core break and root washing methods. Winter wheat root growth dynamics were followed during the growing season using the minirhizotron method. The 2013–2014 results showed that early seeding of wheat improved autumn growth and N uptake and reduced N leaching during the winter compared with the normal seeding time. Early‐seeded wheat (WW_early) was, however, not as efficient as fodder radish at reducing N leaching. Proper establishment of WW_early was a prerequisite for benefiting from early seeding, as indicated by the 2012–2013 results. Early seeding improved root growth throughout the 2013–2014 growing season compared with normal seeding time, but had no significant effect on crop grain yield. Our results indicate the potential of using early seeding as a tool to limit drought susceptibility and increase nutrient uptake from the subsoil.  相似文献   

Analgesic efficacy of intra‐articular morphine in experimentally induced radiocarpal synovitis in horses     

Casper Lindegaard  Maj H Thomsen  Stig Larsen  Pia H Andersen 《Veterinary anaesthesia and analgesia》2010,37(2):171-185

ObjectiveTo compare the analgesic effect of intra-articular (IA) and intravenous (IV) morphine in horses with experimentally induced synovitis.AnimalsEight adult horses.Study designRandomized, observer blinded, double dummy trial with sequential crossover design.MethodsRadiocarpal synovitis was induced by IA injection of lipopolysaccharide on two occasions separated by a 3-week washout period. In one study period horses received treatment IA; morphine IA (0.05 mg kg^?1) plus saline IV and in the other study period they received treatment IV; saline IA plus morphine IV (0.05 mg kg^?1). Lameness and pain were evaluated repeatedly by two observers throughout each of the two 168-hour study periods. Pain was evaluated by use of a visual analogue scale of pain intensity (VAS) and a composite measure pain scale (CMPS). Comparison of treatments was performed by analysis of variance with repeated measurements. Significance level was set to p ≤ 0.05. Inter-observer agreement and agreement between the VAS and CMPS was assessed by use of the Bland–Altman method.ResultsIntra-articular injection of LPS elicited a marked synovitis resulting in lameness and pain. IA morphine resulted in significantly less lameness than IV morphine (p = 0.03). CMPS (p = 0.09) and VAS (p = 0.10) pain scores did not differ significantly between treatments. Inter-observer agreement of the CMPS was classified as good, but only fair for the VAS. Agreement between the two pain scales was considered fair.Conclusions and clinical relevanceAn analgesic effect of IA morphine was demonstrated by significantly reduced lameness scores. The results support the common practice of including IA morphine in a multimodal analgesic protocol after arthroscopic surgery, although further studies in clinical cases are needed. The employed CMPS had good reproducibility, and was easy to use, but may have limited sensitivity at mild intensity pain.  相似文献   

The association between farmers’ participation in herd health programmes and their behaviour concerning treatment of mild clinical mastitis     

Ann-Kristina Lind  Peter T Thomsen  Simo Rintakoski  Mari N Espetvedt  Cecilia Wolff  Hans Houe 《Acta veterinaria Scandinavica》2012,54(1):62

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Feed and strain effects on the use of outdoor areas by broilers     

Nielsen BL  Thomsen MG  Sørensen JP  Young JF 《British poultry science》2003,44(2):161-169

1. The use of outdoor areas by two broiler strains, known to have different growth rates and with access to either moderate (M) or low (L) energy feeds, was examined to assess the consequences for production, meat quality, health and welfare. 2. Sixteen groups of either Ross 208 (ROSS) or a Labresse cross (LAB) were fed either feed M or L. They were raised indoors until 42 d of age when groups of 102 birds were moved to outdoor houses with free access to an outdoor area. 3. The number of chickens standing and lying, respectively, in each of 5 zones in the outdoor area were recorded at intervals throughout the outdoor growth period until slaughter at 84 d of age. Production variables were measured, and gait, feather condition, litter quality, and dermal lesions on foot-pads and hocks were scored together with objective meat quality measures. 4. Both strains showed a diurnal rhythm with most birds observed outside around sunrise and before sunset. Feed and strain both affected the distribution of the birds on the outdoor area, with more birds on feed M than L observed outside, and with more LAB birds outside and using more of the outdoor area than ROSS. This was reflected in some of the carcase and meat quality measures. Feather pecking and cannibalism only occurred in LAB. 5. ROSS was found to have a faster growth rate, poorer litter quality, more dermal lesions and impaired mobility, reflected in low usage of outdoor area and poor gait score compared with LAB. 6. This together with the presence of pectoral myopathies make ROSS unsuitable for 12 week growth in free range production systems. The occurrence of feather pecking and cannibalism in LAB make this particular Labresse cross questionable for meat-type poultry production.  相似文献   

Accuracy of ultrasound-guided intra-articular injection of cervical facet joints in horses: a cadaveric study     

Nielsen JV  Berg LC  Thoefnert MB  Thomsen PD 《Equine veterinary journal》2003,35(7):657-661

REASONS FOR PERFORMING STUDY: Intra-articular facet joint injection is an established diagnostic procedure in human medicine but there are no reports on its reliability in equine practice. OBJECTIVES: To investigate the accuracy of ultrasound-guided intra-articular injections of the cervical facet joints and to estimate factors influencing the accuracy. METHODS: Sixty injections with blue dye were performed on the facet joints between 2nd and 7th cervical vertebra (C2-C7) on horses subjected to euthanasia for nonorthopaedic reasons. The facet joints were subsequently dissected to verify accuracy of deposition. RESULTS: Seventy-two percent of the injections were found to be intra-articular, 17% were intracapsular and a total of 98% were within 1 mm of the joint capsule. There was a marked effect of gained experience (P < 0.01), but not of other factors tested. CONCLUSIONS AND POTENTIAL RELEVANCE: The results of the present study do not translate directly to injections performed in live horses, but they indicate that the method can be applied as a diagnostic as well as therapeutic procedure in C2 to C7 and that is advisable to practise injections on cadaver specimens before applying the technique.  相似文献   

Comparison of two automated immunohistochemical procedures for the diagnosis of scrapie in domestic sheep and chronic wasting disease in North American white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus).     

T V Baszler  M Kiupel  E S Williams  B V Thomsen  T Gidlewski  D L Montgomery  K I O'Rourke  S M Hall 《Journal of veterinary diagnostic investigation》2006,18(2):147-155

Two commercially available automated immunohistochemistry platforms, Ventana NexES and DakoCytomation Autostainer Universal Staining System, were compared for diagnosing sheep scrapie and cervid chronic wasting disease. Both automated platforms used the same antiprion protein monoclonal primary antibodies, but different platform-specific linker and amplification reagents and procedures. Duplicate sections of brainstem (at the level of the obex) and lymphoid tissue (retropharyngeal lymph node or tonsil) from the same tissue block were immunostained for the comparison. Examination of 1,020 tissues from 796 sheep revealed 100% concordance of results between the Ventana NexES and DakoCytomation platforms for diagnosing sheep scrapie from lymphoid tissue (103/103 positive; 405/405 negative) and brainstem (120/120 positive; 392/392 negative). Similarly, examination of 1,008 tissues from 504 white-tailed deer revealed 100% concordance between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease from lymphoid tissue (104/104 positive; 400/400 negative) and brainstem (104/104 positive; 400/400 negative). Examination of 1,152 tissues from 482 mule deer revealed a concordance of 98.6% in lymphoid tissue and 99.9% in brainstem between the Ventana NexES and DakoCytomation platforms for diagnosing chronic wasting disease. The results indicate equivalence or near equivalence between the DakoCytomation and Ventana NexES autostainer platforms for identification of the disease-associated prion protein (PrPd)-positive and PrPd-negative brain and lymphoid tissues in sheep, white-tailed deer, and mule deer.  相似文献