A novel in vitro hydroponic culture system for potato (Solanum tuberosum L.) microtuber production     

Duong Tan Nhut  Nguyen Hoang NguyenDang Thi Thu Thuy 《Scientia Horticulturae》2006

An innovative in vitro hydroponic culture system used in potato (Solanum tuberosum L.) microtuber production is described in this paper. In vitro potato plantlets, 6–8 cm in height, derived from meristems of potato tubers cultured on 1/2 Murashige and Skoog (MS) nutrient medium after 30 days culture were cut into 1.5 cm stem node segments and used as explants. These stem nodes were cultured in a novel system called in vitro hydroponic culture system containing 1/2 MS medium supplemented with 0.5 μM naphthaleneacetic acid (NAA), 0.3 μM gibberellic acid (GA₃), 3.7 μM adenine sulfate, 10% coconut water, 0.5 g/l activated charcoal, 80 g/l sucrose with or without 8 g l⁻¹ agar. Liquid medium was distributed to the carrier substrates in each storey of the system with the aid of capillary robes. In the present paper, the effects of porous material used as substrate carrier and the number of storeys involved in the culture system on microtuber formation and their morphological characteristics are reported. Cotton layer substrate is more stable for organogenesis of potato microtubers. Microtubers, 3.19 mm in diameter and 49.82 mg in weight, could be harvested from a one-storey in vitro hydroponic culture system containing filter paper as substrate. However, microtubers cropped from three-storey in vitro hydroponic culture system with cotton layer were bigger and weightier than those from three-storey system containing filter paper. The above results of the in vitro hydroponic system examined in this study might open up a new approach in producing potato and other hygrophilous microtuber.  相似文献   

Ethanol-soluble components in soybean meal influence the digestive physiology,hepatic and intestinal morphologies,and growth performance of the marine fish pompano (Trachinotus blochii)     

Hung Phuc Nguyen  Thinh Van Do  Nang Thu Tran Thi  Trung Anh Trieu 《Journal of animal physiology and animal nutrition》2021,105(4):766-776

This study aimed to examine the effects of ethanol-soluble components (Es) in soybean meal (SBM) on gut content transit, bile acid (BA) and pancreatic digestive enzyme secretions, nutrient apparent digestibility coefficients (ADC), liver and intestinal morphologies, and growth performance of pompano (Trachinotus blochii). The SBM was extracted with aqueous ethanol, then the supernatant and residue were separated and dried to produce ethanol-extracted SBM (ESBM) and the Es. Four experimental diets were formulated with fish meal (FM), ESBM and SBM as main dietary protein sources. The diets were denoted as follows: FMD (FM diet), SBMD (SBM diet), ESBMD (ESBM diet) and ESBM + EsD (ESBM plus Es diet). Thirty-five fingerling pompano with an initial body weight (BW) of 18.3 g were allocated to each of 12 polyvinyl chloride tanks (1000-L holding capacity), resulting in triplicate tanks per dietary treatment. For 10 weeks, the fish were hand-fed the experimental diets to apparent satiation twice daily. The results showed that the final BW, weight gain and specific growth rate were significantly lower, while the feed conversion ratio was higher in the SBMD and ESBM + EsD groups than in the ESBMD and FMD groups (p < 0.05). Fish fed SBMD and ESBM + EsD showed accelerated gastric transit, slowed intestinal mobility, and lowered secretions of BAs and pancreatic digestive enzymes as compared to those fed ESBMD and FMD. Morphological abnormalities in mucosal folds of the posterior intestine, but not the liver, were clearly observed in the SBMD and ESBM + EsD groups. These results indicated that the Es in SBM inhibited the digestive system, leading to decreased nutrient digestibility and growth performance in pompano. The findings of the present study suggested that removal of the Es would effectively improve the nutritional quality of SBM and enhance growth performance of pompano fed a SBM-based diet.  相似文献   

Disruption of duplicated yellow genes in Bactrocera tryoni modifies pigmentation colouration and impacts behaviour     

Nguyen  Thu N. M.  Mendez  Vivian  Ward  Christopher  Crisp  Peter  Papanicolaou  Alexie  Choo  Amanda  Taylor  Phillip W.  Baxter  Simon W. 《Journal of pest science》2021,94(3):917-932

Journal of Pest Science - Irradiated Queensland fruit flies (Bactrocera tryoni) used in Sterile Insect Technique (SIT) programmes are marked with fluorescent dyes to distinguish them from wild...  相似文献   

Correlation of the antinociceptive action and plasma concentration of fentanyl following intravenous administration in cats     

SA Robertson  WL Davies  PM Taylor  MJ Dixon 《Veterinary anaesthesia and analgesia》2002,29(2):94-94

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Spatial epidemiology of human anthrax in Son La province,Vietnam, 2003–2022     

Tan Luong  Tien Dung Nguyen  Van Truong Lu  Morgan C. Metrailer  Van Khang Pham  Thi Thu Ha Hoang  Thi Mai Hung Tran  Thanh Hai Pham  Thanh Long Pham  Quang Thai Pham  Jason K. Blackburn 《Zoonoses and public health》2024,71(4):392-401

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Effects of trees planted on levees on rice yields in rain-fed paddy fields of northeast Thailand     

Shuichi Miyagawa  Manami Kobayashi  Ha Thu Pham 《Plant Production Science》2017,20(1):47-54

Trees are increasingly being planted on the levees of paddy fields of rice (Oryza sativa L.) in northeast Thailand. We investigated and compared the yields of rice grown in rain-fed paddies under and far from canopies of three different tree species: eucalyptus (Eucalyptus spp.), mango (Mangifera indica L.), and the indigenous neem tree (Azadirachta indica A. Juss). Rice yields tended to decrease near trees of all types at five sites, but there was no change in yields at the remaining 11 sites during the 3-year study. The reduced yields likely resulted from lower aboveground biomass, leading to fewer rice panicles and spikelets, particularly near trees with a dense canopy. Extremely low yields were observed near eucalyptus in paddy fields suffering from severe drought. These results, as well as information provided by farmers’, suggest that eucalyptus trees may have detrimental effects on rice and should not be planted on the levees of paddy fields with relatively low productivity.  相似文献