Randomized clinical trial to evaluate the effectiveness of enrofloxacin as a second‐line antibiotic for treatment of acute Escherichia coli mastitis          下载免费PDF全文

Kazuhiro Kawai  Akira Takeda  Michiaki Yamada  Fumi Kayasaki  Nao Kondo  Yoshiko Sasaki  Nahoko Kanai  Takuma Mukai  Naoki Sawaguchi  Masaya Higuchi  Haruka Kondo  Kouki Sugimoto  Akane Yasuda  Aiko Watanabe 《Animal Science Journal》2018,89(7):1033-1039

The objective of the present study was to evaluate the effectiveness of enrofloxacin (ERFX) as a second‐line antibiotic for treatment of acute Escherichia coli (E. coli) mastitis. Forty‐two cows with naturally occurring acute E. coli mastitis were enrolled. On the first day of treatment (day 0), empirically selected antibiotics (oxytetracycline: n = 32, kanamycin: n = 10) were administered. Although systemic signs improved in 10 cows (first‐line group), the signs remained unchanged or worsened in 32 cows on day 1, including two cows that were found dead. The 30 surviving cows were randomly assigned to second‐line groups constituting an ERFX group (n = 19) or a control group (n = 11) that was treated with other antibiotics. Response to each treatment was evaluated by measuring clinical signs from day 0 to day 3, subsequent quarter milk recovery, and the 60‐day survival rate. Appetite on day 3 was significantly better in the ERFX group compared to the control group. No significant differences were observed in the 60‐day survival rate or the subsequent milk recovery between the ERFX group and the control group. Thus, the use of ERFX as a second‐line antibiotic for the treatment of acute E. coli mastitis could induce a rapid appetite recovery.  相似文献   

Estimation of sequestered carbon in Article-3.4 private planted forests in the first commitment period in Japan     

Takuya Hiroshima  Tohru Nakajima 《Journal of Forest Research》2006,11(6):427-437

The purpose of this study was to predict the likely amounts of carbon sequestration on a national scale for Japan in the Article-3.4 private planted forests of the Kyoto Protocol during the first commitment period. We regarded the planted forests that had undergone silvicultural practices such as weeding, pruning, and thinning since 1990 as Article-3.4 planted forests in accordance with the definition given by the Forestry Agency of Japan. Regression models were developed to predict the forest areas that had undergone silvicultural practices, employing silvicultural subsidies and forest workers' wages as predictor variables. Then the time series changes in the predictor variables were provided by extending their recent trends, with the result being that the forest areas that have undergone silvicultural practices were predicted on the basis of the three scenarios of the variables. Thus, the Article-3.4 forest area was calculated considering overlaps of silvicultural practices over fixed stands, and the area was converted into the amount of carbon sequestration by multiplying it by coefficients such as a volume table, biomass expansion factor, and others. The result implied that Article-3.4 private planted forests were expected to sequester 8.16–8.87 Mt-C year⁻¹ during the first commitment period. These amounts cover 63%–68% of the carbon sequestration goal by land-use change and forestry activities capped under the Marrakesh Accords. To realize this prediction, it is important to provide a sufficient silvicultural subsidy to last until the end of the first commitment period and to implement silvicultural practices on the forest stands that have not undergone such practices since 1990.  相似文献   

Condensation reactions of phenolic resins VII: catalytic effect of sodium bicarbonate for the condensation of hydroxymethylphenols     

Naruyuki Kamo  Jun Tanaka  Mitsuo Higuchi  Tetsuo Kondo  Mitsuhiro Morita 《Journal of Wood Science》2006,52(4):325-330

This article describes the catalytic effect of NaHCO₃ on condensation reactions of monomeric hydroxymethylphenols (HMPs) to elucidate the cure-acceleration mechanism. By comparison of the kinetics of self-condensations of HMPs, NaHCO₃ was proved to increase the reactivity of para-hydroxymethyl groups. The changes of ¹³C nuclear magnetic resonance (NMR) chemical shifts on each HMP system with the additive indicated that the addition of NaHCO₃ enhanced some molecular interactions between HMPs and NaHCO₃, facilitating a resonance effect that might play a similar role in dissociation of the phenolic hydroxyl groups of HMPs. In addition, computational modeling by molecular orbital calculations elucidated that hydrogen carbonate anion (HCO ₃ ⁻ ) forms an interaction between either the para-hydroxymethyl group and the phenolic hydroxyl group or between the two para-hydroxymethyl groups of HMPs by hydrogen bonds. From the experimental results, the authors proposed the mechanism of the catalytic action of NaHCO₃: it appears to be due to the delocalization of an electron initiated by the interaction of the para-hydroxymethyl groups and the phenolic hydroxyl of HMPs with HCO ₃ ⁻ through hydrogen bonds, which results in facilitating the formation of active species.  相似文献   

Flonicamid, a novel insecticide with a rapid inhibitory effect on aphid feeding     

Morita M  Ueda T  Yoneda T  Koyanagi T  Haga T 《Pest management science》2007,63(10):969-973

Flonicamid (IKI220; N-cyanomethyl-4-trifluoromethylnicotinamide), a pyridinecarboxamide compound, is a novel systemic insecticide with selective activity against hemipterous pests, such as aphids and whiteflies, and thysanopterous pests. The purpose of this study is to clarify the biological properties of flonicamid against aphids. Flonicamid is very active against aphids, regardless of differences in species, stages and morphs. This compound inhibited the feeding behaviour of aphids within 0.5 h of treatment without noticeable poisoning symptoms such as convulsion, and this antifeeding activity was not recoverable until death. The nymphs born from adults exposed to flonicamid for 3 h showed high mortality. The effect of flonicamid on the feeding activity of an individual aphid was studied using electronic monitoring of insect feeding behaviour (EMIF). Although the treated aphid attached the head of its proboscis to the leaf surface, salivation and sap feeding were strongly inhibited. These results suggest that the main insecticidal mechanism of flonicamid is starvation based on the inhibition of stylet penetration to plant tissues.  相似文献   

Peptides derived from dolicholin, a phaseolin-like protein in country beans (Dolichos lablab), potently stimulate cholecystokinin secretion from enteroendocrine STC-1 cells     

Sufian MK  Hira T  Asano K  Hara H 《Journal of agricultural and food chemistry》2007,55(22):8980-8986

Peptides derived from soybean beta-conglycinin and pork protein stimulate cholecystokinin (CCK) secretion from the enteroendocrine cells (EECs) and suppress food intake. Here we examined CCK-releasing activities from the enteroendocrine cell line STC-1, in peptides derived from underutilized legumes, and found much higher activity in the peptic hydrolysate of Country beans (CBP) compared to that from other legume-derived peptides including beta-conglycinin peptone. Active components in CBP were separated into acetonitrile-soluble fractions, but the activities were abolished after pronase treatment. To identify the Country bean protein containing the active peptides, Country bean protein extracts in an alkaline solution (CBE) were fractionated based on isoelectric point or molecular weight. Peptones prepared from CBE fractions containing a 51 kDa major protein stimulated CCK release, but other fractions did not. N-Terminal sequence analysis indicated that the 51 kDa protein is a phaseolin-like globular protein, and we designated this protein dolicholin. These results indicate that Country bean-derived peptides are very potent legume peptides in stimulating CCK secretion from EECs and that the stimulant peptides originate from dolicholin, a newly identified phaseolin-like globular protein in Country beans.  相似文献   

Role of serum myostatin during the lactation period   总被引：1，自引：0，他引：1  

Hosoyama T  Yamanouchi K  Nishihara M 《The Journal of reproduction and development》2006,52(4):469-478

Myostatin, also known as GDF-8 (Growth/Differentiation Factor-8), is a member of the TGF-beta superfamily that negatively regulates skeletal muscle mass in mammals. Mutation of the myostatin gene in mice, cattle, and humans causes a massively developed skeletal muscle, characterized by muscle hypertrophy and hyperplasia. Although myostatin is predominantly expressed in skeletal muscle tissue, several recent studies have shown the presence of myostatin protein in blood and suggested a possible role for circulating myostatin in the regulation of skeletal muscle mass. In the present study, we examined changes in the levels of active form myostatin (13 kDa) in serum after birth by Western blot analysis to predict the role of serum myostatin in early postnatal muscle growth in the rat. Interestingly, the amount of active form myostatin in serum increased after birth and then decreased along with ageing after weaning. To clarify the role of increased serum myostatin during the postnatal period, we administrated follistatin, an inhibitor of myostatin activity, into postnatal rats intraperitoneally just after birth. Follistatin-administration during the postnatal period caused selective hypertrophy of type II muscle fibers in the soleus muscle. These results demonstrate that myostatin in serum acts on skeletal muscle and negatively regulates early postnatal muscle growth.  相似文献   

Detailed images of asteroid 25143 Itokawa from Hayabusa     

Saito J  Miyamoto H  Nakamura R  Ishiguro M  Michikami T  Nakamura AM  Demura H  Sasaki S  Hirata N  Honda C  Yamamoto A  Yokota Y  Fuse T  Yoshida F  Tholen DJ  Gaskell RW  Hashimoto T  Kubota T  Higuchi Y  Nakamura T  Smith P  Hiraoka K  Honda T  Kobayashi S  Furuya M  Matsumoto N  Nemoto E  Yukishita A  Kitazato K  Dermawan B  Sogame A  Terazono J  Shinohara C  Akiyama H 《Science (New York, N.Y.)》2006,312(5778):1341-1344

Rendezvous of the Japanese spacecraft Hayabusa with the near-Earth asteroid 25143 Itokawa took place during the interval September through November 2005. The onboard camera imaged the solid surface of this tiny asteroid (535 meters by 294 meters by 209 meters) with a spatial resolution of 70 centimeters per pixel, revealing diverse surface morphologies. Unlike previously explored asteroids, the surface of Itokawa reveals both rough and smooth terrains. Craters generally show unclear morphologies. Numerous boulders on Itokawa's surface suggest a rubble-pile structure.  相似文献   

Cytokinin signaling and its inhibitor AHP6 regulate cell fate during vascular development     

Mähönen AP  Bishopp A  Higuchi M  Nieminen KM  Kinoshita K  Törmäkangas K  Ikeda Y  Oka A  Kakimoto T  Helariutta Y 《Science (New York, N.Y.)》2006,311(5757):94-98

The cell lineages that form the transporting tissues (xylem and phloem) and the intervening pluripotent procambial tissue originate from stem cells near the root tip. We demonstrate that in Arabidopsis, cytokinin phytohormones negatively regulate protoxylem specification. AHP6, an inhibitory pseudophosphotransfer protein, counteracts cytokinin signaling, allowing protoxylem formation. Conversely, cytokinin signaling negatively regulates the spatial domain of AHP6 expression. Thus, by controlling the identity of cell lineages, the reciprocal interaction of cytokinin signaling and its spatially specific modulator regulates proliferation and differentiation of cell lineages during vascular development, demonstrating a previously unrecognized regulatory circuit underlying meristem organization.  相似文献   

Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life     

Yoshihiro Okabe  Erika Asamizu  Tohru Ariizumi  Kenta Shirasawa  Satoshi Tabata  Hiroshi Ezura 《Breeding Science》2012,62(2):202-208

Novel mutant alleles of an ethylene receptor Solanum lycopersicum ETHYLENE RESPONSE1 (SlETR1) gene, Sletr1-1 and Sletr1-2, were isolated from the Micro-Tom mutant library by TILLING in our previous study. They displayed different levels of impaired fruit ripening phenotype, suggesting that these alleles could be a valuable breeding material for improving shelf life of tomato fruit. To conduct practical use of the Sletr1 alleles in tomato breeding, genetic complementation analysis by transformation of genes carrying each allele is required. In this study, we generated and characterized transgenic lines over-expressing Sletr1-1 and Sletr1-2. All transgenic lines displayed ethylene insensitive phenotype and ripening inhibition, indicating that Sletr1-1 and Sletr1-2 associate with the ethylene insensitive phenotype. The level of ethylene sensitivity in the seedling was different between Sletr1-1 and Sletr1-2 transgenic lines, whereas no apparent difference was observed in fruit ripening phenotype. These results suggested that it is difficult to fine-tune the extent of ripening by transgenic approach even if the weaker allele (Sletr1-2) was used. Our present and previous studies indicate that the Micro-Tom mutant library combined with TILLING could be an efficient tool for exploring genetic variations of important agronomic traits in tomato breeding.  相似文献   

Dietary phytase increases the true absorption and endogenous fecal excretion of zinc in growing pigs given a corn-soybean meal based diet     

Gyo-Moon CHU  Motoharu KOMORI  Ryota HATTORI  Tohru MATSUI 《Animal Science Journal》2009,80(1):46-51

We investigated the effect of dietary phytase on the true absorption and endogenous fecal excretion of zinc (Zn) using ⁶⁷Zn in growing pigs given a corn-soybean meal based diet. Ten crossbred barrows were fed the control diet containing 90-mg/kg Zn, 2.3-g/kg phytate-phosphorus and 3.7-g/kg non-phytate-phosphorus or the phytase diet containing similar amounts of Zn and phytate-phosphorus, and 1.4-g/kg non-phytate-phosphorus with 750-PU/kg phytase for 12 h/day. On day 6, the pigs were given 200 g of the corresponding diet labeled by ⁶⁷Zn for 2 h. We measured feed intake, fecal Zn concentration and ⁶⁷Zn abundance for the determination of apparent absorption, true absorption and endogenous fecal excretion of Zn. Although the apparent absorption of Zn did not significantly differ between the dietary groups, the phytase group had significantly more ( P  < 0.05) true absorption of Zn than the control group. The endogenous fecal excretion of Zn tended to be more ( P  = 0.07) in the phytase group than in the control group. These results suggest that dietary phytase improves Zn bioavailability through increasing the true absorption of Zn in growing pigs, which results in stimulating the endogenous fecal excretion of Zn when dietary Zn satisfies its requirement.  相似文献