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71.
Natsumi ENDO Kiyosuke NAGAI Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2013,59(2):107-114
The present study was designed to assess progesterone profiles at the secreted (caudal
vena cava) and circulating levels (jugular vein) and luteinizing hormone (LH) secretion
pattern in lactating and non-lactating cows with reference to feeding. Four lactating and
four non-lactating cycling Holstein cows were examined. Blood samples were collected
simultaneously from the caudal vena cava (via a catheter inserted from the coccygeal vein)
and the jugular vein every 15 min for 12 h (0500–1700 h) during the functional luteal
phase. Cows were fed 50% of the daily diet 6 h after the start of blood sampling. During
the 12-h sampling period, mean progesterone concentrations in the caudal vena cava did not
differ between lactating and non-lactating cows (49.0 ± 2.9 and 53.3 ± 3.7 ng/ml; mean ±
SE), whereas mean progesterone concentrations in the jugular vein in lactating cows were
higher than those in non-lactating cows (6.4 ± 0.1 and 5.6 ± 0.1 ng/ml, P < 0.001).
Lactating cows had a higher frequency of LH pulses than non-lactating cows (7.0 ± 0.7 and
4.3 ± 0.9 pulses/12 h, P<0.05). The influence of feeding was not observed on LH
profiles but was observed on progesterone profiles in both veins. Progesterone
concentrations in the caudal vena cava increased after feeding in both groups.
Progesterone concentrations in the jugular vein decreased after feeding in lactating cows
but not in non-lactating cows. These results indicate the difference in feeding-related
changes in progesterone dynamics between lactating and non-lactating cows. 相似文献
72.
Tomomi TANAKA Satoshi OHKURA Yoshihiro WAKABAYASHI Takenobu KUROIWA Kiyosuke NAGAI Natsumi ENDO Akira TANAKA Hisanori MATSUI Masami KUSAKA Hiroaki OKAMURA 《The Journal of reproduction and development》2013,59(6):563-568
The aim of the present study was to determine if the estradiol-induced luteinizing
hormone (LH) surge is influenced by the constant exposure to TAK-683, an
investigational metastin/kisspeptin analog, that had been established to depress the
pulsatile gonadotropin-releasing hormone (GnRH) and LH secretion in goats.
Ovariectomized goats subcutaneously received TAK-683 (TAK-683 group, n=6) or vehicle
(control group, n=6) constantly via subcutaneous implantation of an osmotic pump.
Five days after the start of the treatment, estradiol was infused intravenously in
both groups to evaluate the effects on the LH surge. Blood samples were collected at
6-min intervals for 4 h prior to the initiation of either the TAK-683 treatment or
the estradiol infusion, to determine the profiles of pulsatile LH secretion. They
were also collected at 2-h intervals from –4 h to 32 h after the start of estradiol
infusion for analysis of LH surges. The frequency and mean concentrations of LH
pulses in the TAK-683 group were remarkably suppressed 5 days after the start of
TAK-683 treatment compared with those of the control group (P<0.05). On the other
hand, a clear LH surge was observed in all animals of both groups. There were no
significant differences in the LH concentrations for surge peak and the peak time of
the LH surge between the TAK-683 and control groups. These findings suggest that the
effects of continuous exposure to kisspeptin or its analog on the mechanism(s) that
regulates the pulsatile and surge mode secretion of GnRH/LH are different in
goats. 相似文献
73.
Toru Kondo Mamoru Satou Toshiyuki Morikawa Yoshiaki Chikuo 《Journal of General Plant Pathology》2014,80(1):99-102
Brown root rot of Russell prairie gentian was observed in the Aomori Prefecture, Japan in April 2011. The fungal isolate from the diseased root was identified as Subplenodomus drobnjacensis on the basis of its morphological characteristics and nucleotide sequences. The isolate induced similar root rot symptoms when inoculated in healthy Russell prairie gentian plants. We proposed the name “brown root rot” for this disease. 相似文献
74.
Rumi Kaida Tomomi Kaku Kei’ichi Baba Masafumi Oyadomari Takashi Watanabe Sri Hartati Enny Sudarmonowati Takahisa Hayashi 《Journal of Wood Science》2009,55(5):381-386
We examined the saccharification and fermentation of meals from Acacia mangium wood, Paraserianthes falcataria wood, and Elaeis guineensis trunk. The levels of enzymatic hydrolysis of cellulose and ethanol production were highest for P. falcataria wood and lowest for A. mangium wood. Ultrasonication pretreatment of meal further increased the rates of hydrolysis and ethanol production in meal from
P. falcataria wood. Through this pretreatment, hemicelluloses (xylan and xyloglucan) and cellulose were released in the meal from P. falcataria wood. Loosening of hemicellulose associations can be expected to make P. falcataria wood more useful for bioethanol production. 相似文献
75.
Comparison of productive and reproductive performance and hair cortisol levels between Brown Swiss cross‐bred and Holstein cows housed in the same barn 下载免费PDF全文
The productive and reproductive characteristics of Brown Swiss (B) cross‐bred cows were investigated by comparing with those of Holstein (H) cows housed in the same barn. Additionally, their hair cortisol levels were analyzed to evaluate the extent of stress experienced during dry and lactation periods. B cross‐bred cows had lower milk yields and higher milk fat rates than H cows. Reproductive records showed that days from parturition to first artificial insemination (AI) in B cross‐bred (n = 16) and H (n = 27) cows were not significantly different, but conception rate at first AI of B cross‐bred cows tended to be higher than that of H cows. Percentage of B cross‐bred cows that resumed ovarian cyclic activity within 45 days after parturition was higher than that of H cows (6/6 (100%) and 5/11 (45.5%), P < 0.05), and B cross‐bred cows had higher body condition scores at that time. Hair cortisol level at 60 to 90 days after parturition in H cows increased significantly compared with in the dry period, and it was higher than that of B cows during the same period. These results suggest that B cross‐bred cows experience less metabolic stress during early lactation, which may result in earlier resumption of reproductive function. 相似文献
76.
Morikawa K Nonaka M Mochizuki H Handa K Hanada H Hirota K 《Journal of agricultural and food chemistry》2008,56(22):11030-11037
Citrus flavonoids are reported to be promising bioactive compounds against hyperlipidemia and lipid biosynthesis. However, the mechanism of the lipid lowering effect by flavonoids remains unknown. The present study examines the effect of some flavanones on the adipocytic conversion of the human preadipocyte cell line, AML-I. Among four structure-related flavanones including naringenin, naringenin-7-rhamnoglucoside (naringin), hesperetin, and hesperetin-7-rhamnoglucoside (hesperidin), the aglycones such as naringenin and hesperetin exhibited the growth arrest of AML-I cells. When the cells were examined by Annexin V-FITC staining method, it was noticed that growth arrest was induced by apoptotic cell death. In the study of apoptosis-related protein in the naringenin-treated cells, anti-apoptotic proteins such as p-Akt, NF-kappaB, and Bcl-2 were decreased, and pro-apoptotic protein Bad was accumulated by Western blot analysis. Interestingly, exposure of AML-I cells to naringenin or hesperetin during short-term cultures increased cytoplasmic lipid droplets by Sudan Black B staining. Furthermore, expression of fatty acid synthase (FAS) and peroxisome proliferator activated receptor (PPAR)-gamma was enhanced in naringenin-treated cells. These data suggest that apoptosis by flavanones does not inhibit the adipocytic conversion of AML-I preadipocytes. The result also indicates that adipocyte may not be a direct target for the lipid-lowering activity of the flavanones. 相似文献
77.
Kazuhiro KENGAKU Tomomi TANAKA Hideo KAMOMAE 《The Journal of reproduction and development》2021,67(5):332
The aim of the present study was to clarify the ovarian and hormonal dynamics after the aspiration of follicular fluid in cows with follicular cysts. Follicular fluid was aspirated from the follicular cysts and follicles that were fated to become cystic follicles and other coexisting normal follicles, respectively, in lactating cows (n = 3). After the aspiration procedure, new follicles developed and reached a diameter of 25 mm without ovulation within 13–19 days. The plasma concentrations of inhibin decreased and follicle-stimulating hormone increased rapidly after the aspiration procedure, and subsequently increased and decreased, respectively, as a new follicle grew. No luteal structures developed after the aspiration procedure, and the animals’ plasma progesterone levels remained low. The present study indicates that the cystic follicles are never luteinized by the aspiration of follicular fluid, and consequently, new follicular cysts are observed to repeatedly develop. 相似文献
78.
Soybean is often damaged by hypoxia caused by waterlogging at the seedling stage. Hypoxia severely inhibits root development and retards plant growth. We aimed to clarify phenotypic variation in root development under hypoxia condition at the seedling stage using diverse soybean accessions. Root development in 162 accessions was evaluated in hydroponic culture. Substantial changes under hypoxia were investigated by means of WinRHIZO analysis before and after the treatment. We found significant phenotypic variation in hypoxia tolerance in root among the 162 accessions. A principal components analysis indicated an association between hypoxia tolerance and the country of origin. We found three new accessions which have a high ability to develop roots under hypoxia (Kokubu 7, Maetsue zairai 90B, and Yahagi). Root development in selected accessions was also evaluated in soil culture. Root development levels in hydroponic and soil culture were significantly correlated. These results will provide important information on waterlogging damage in regions where waterlogging occurs. The three accessions with hypoxia-tolerant roots might be useful for genetic improvement of waterlogging tolerance of modern soybean varieties. 相似文献
79.
This research presents a simple way to enhance the anti-UV property of bamboo pulp fabric trough in situ synthesis of ZnO particles on the fabric. Bamboo Pulp Fabric was treated by immersion method in Zn(NO3)2 and multi-amide compound (RSD) aqueous solution under hydrothermal condition, then the ZnO particles were grown on the surface of bamboo pulp fabric by in-situ method. In this synthesis RSD was applied as a stabilizer, reactant and dispersant agent, the reaction mechanism was investigated. The ZnO particles were characterized by transmission electron microscope (TEM), Xray diffraction (XRD) and ultraviolet/visible light spectrophotometer. The anti-UV property of Bamboo Pulp Fabric was characterized by measuring its UPF. The results indicated that the diameter of ZnO is about 150 nm, the length is about 600 nm. The Bamboo Pulp Fabric treated with ZnO particles showed good anti-UV property and its UPF can reach to 83.59. After washing for 20 times, it can also keep good anti-UV property. 相似文献
80.
Kawakami E Ozawa T Hirano T Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):83-85
The right testis and epididymis were excised from a Beagle dog that ejaculated high percentages of sperm with detached tails and with coiled tails. Cross sections of the organs were stamped on glass slides and histological examination of the organs was performed to find the portion where sperm with the abnormal tails appear. Many sperm with tails whose axoneme was exposed near the neck region were observed in the testis and they decreased in order from the caput, to the corpus, and the cauda epididymis. Sperm with detached tails and sperm with coiled tails gradually increased in the epididymis. These findings indicate that the tails of sperm with an exposed axoneme detached in the epididymis. 相似文献