稻鸭共生对鸭肉品质和屠宰性能的影响   总被引：3，自引：0，他引：3  

卢跃红  戴志明  张曦  魏红江  万田正治  中西良孝  韩自鸿 《中国家禽》2009,31(5)

以云南麻鸭为试验材料,分别在稻鸭共生(稻鸭组)和常规网上平养(常规组)两种饲养模式下饲养至体重达1.8 kg,测定其常规肉品质和部分屠宰性能.结果表明:①两组间鸭肉失水率、嫩度、熟肉率、肉色亮度值和红度值无显著差异(P＞0.05);稻鸭组的pH值显著高于常规组(P＜0.05);肉色黄度值B极显著高于常规组(P＜0.01).②两组间屠宰率、半净膛率、全净膛率、胸肉率差异不显著(P＞0.05);稻鸭组的皮脂厚、腹脂率比常规组分别降低了18.52%、11.76%(P＜0.05);腿肉率提高了11.40%(P＜0.05).③稻鸭组的生长速度明显慢于常规组.  相似文献   

Co-culture of carp (Cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages     

Fumihiko Katakura  Fumio Takizawa  Miyuki Yoshida  Takuya Yamaguchi  Kyosuke Araki  Mitsuru Tomana  Miki Nakao  Tadaaki Moritomo  Teruyuki Nakanishi 《Veterinary immunology and immunopathology》2009,131(1-2):127-136

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Cross-reactivities with Cryptosporidium spp. by chicken monoclonal antibodies that recognize avian Eimeria spp     

Matsubayashi M  Kimata I  Iseki M  Lillehoj HS  Matsuda H  Nakanishi T  Tani H  Sasai K  Baba E 《Veterinary parasitology》2005,128(1-2):47-57

In a previous study, we have developed several chicken monoclonal antibodies (mAbs) against Eimeria acervulina (EA) in order to identify potential ligand molecules of Eimeria. One of these mAbs, 6D-12-G10, was found to recognize a conoid antigen of EA sporozoites and significantly inhibited the sporozoite invasions of host T lymphocytes in vitro. Furthermore, some of these chicken mAbs showed cross-reactivities with several different avian Eimeria spp. and the mAb 6D-12-G10 also demonstrated cross-reactivities with the tachyzoites of Neospora caninum and Toxoplasma gondii. Cryptosporidium spp. are coccidian parasites closely related to Eimeria spp., and especially C. parvum is an important cause of diarrhea in human and mammals. In the present study, to assess that the epitopes recognized by these chicken mAbs could exist on Cryptosporidium parasites, we examined the cross-reactivity of these mAbs with Cryptosporidium spp. using an indirect immunofluorescent assay (IFA) and Western blotting analyses. In IFA by chicken mAbs, the mAb 6D-12-G10 only showed a immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum. Western blotting analyses revealed that the mAb 6D-12-G10 reacted with the 48-kDa molecular weight band of C. parvum and C. muris oocyst antigens, 5D-11 reacted the 155 kDa of C. muris. Furthermore, these epitopes appeared to be periodate insensitive. These results indicate that the target antigen recognized by these chicken mAbs might have a shared epitope, which is present on the apical complex of apicomplexan parasites.  相似文献   

An oligonucleotide probe for the detection of Erwinia herbicola and Erwinia ananas     

Yoshihiro Nakanishi  Appolinaire Adandonon  Ikuko Okabe  Yuko T. Hoshino  Naoyuki Matsumoto 《Journal of General Plant Pathology》2006,72(5):328-333

An oligonucleotide probe targeting the rRNA of Erwinia herbicola and Erwinia ananas was designed to detect their cells using fluorescence in situ hybridization. The Cy3-labeled probe hybridized strongly with these species but very weakly with nontarget species such as Erwinia mallotivora, Erwinia nigrifluens, Erwinia cypripedii, Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli. This technique visualized E. herbicola cells after inoculation of kumquat fruits. The probe is promising as a tool for studying population dynamics of E. herbicola and E. ananas.  相似文献   

A binding protein for fungal signal molecules in the cell wall of Pisum sativum     

Akinori Kiba  Takako Ohgawara  Kazuhiro Toyoda  Miho Inoue-Ozaki  Tadahiro Takeda  Uppalapati Srinivasa Rao  Toshiaki Kato  Yuki Ichinose  Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(4):228-237

In the plant cell wall of Pisum sativum seedlings, we found an NTPase (E.C. 3.6.1.5.) with ATP-hydrolyzing activity that was regulated by an elicitor and suppressors of defense from pea pathogen Mycosphaerella pinodes. The ATPase-rich fraction was purified from pea cell walls by NaCl solubilization, ammonium sulfate precipitation, and chromatography with an ATP-conjugated agarose column and an anion-exchange column. The specific activity of the final ATPase-rich fraction increased 600-fold over that of the initial NaCl-solubilized fraction. The purified ATPase-rich fraction also had peroxidase activity and generated superoxide, both of which were regulated by the M. pinodes elicitor and suppressor (supprescins). Active staining and Western blot analysis also showed that the ATPase was copurified along with peroxidases. In this fraction, a biotinylated elicitor and the supprescins were bound primarily and specifically to ca. 55-kDa protein (CWP-55) with an N-terminal amino acid sequence of QEEISSYAVVFDA. The cDNA clone of CWP-55 contained five ACR domains, which are conserved in the apyrases (NTPases), and the protein is identical to a pea NTPase cDNA (GenBank accession AB071369). Based on these results, we discuss a role for the plant cell wall in recognizing exogenous signal molecules.  相似文献   

Participation of different macrophage populations and myofibroblastic cells in chronically developed renal interstitial fibrosis after cisplatin-induced renal injury in rats   总被引：5，自引：0，他引：5  

Yamate J  Sato K  Ide M  Nakanishi M  Kuwamura M  Sakuma S  Nakatsuji S 《Veterinary pathology》2002,39(3):322-333

To shed some light on the mechanisms behind renal fibrogenesis, the present study immunohistochemically investigated the participation of different macrophage populations and myofibroblastic cells in rat renal interstitial fibrosis developed chronically after repeated injection of cisplatin (2 mg/kg body weight, once weekly for 7 weeks). During the 19-week recovery period after the final injection, fibrotic lesions progressively developed in the corticomedullary junction, with the greatest level at post-final injection (FPI) week 5, and then the lesions were gradually repaired by PFI week 19, indicative of a healing process. In conformity with the development of fibrotic lesions, the number of myofibroblastic cells reacting with an anti-alpha-smooth muscle actin antibody was increased, with a peak at PFI week 3, and collagens (types I, III, and IV), fibronection, and laminin were excessively accumulated in these areas. Interstitial cells forming the fibrotic lesions showed mitotic activity at the early stages, whereas they disappeared by apoptosis in the healing process. A large number of cells reacting with an antibody of ED1 (for exudate macrophages), ED2 (for resident macrophages), or OX6 (for major histocompatibility complex class II-presenting macrophages and interstitial dendritic cells) had already appeared at PF1 week 1, and then their numbers increased, with a peak at PFI weeks 7, 3, and 9 in ED1-, ED2-, and OX6-positive cells, respectively. Thereafter, the number of ED1- and ED2-positive cells decreased, whereas the number of OX6-positive cells persisted at a high level until PFI week 19. In the healing process, clusters of lymphocytes were present, the development of which might have been related to OX6-positive cells. The present study demonstrated that chronically developing rat renal interstitial fibrosis might be produced by the complicated mechanisms evoked by interactions between different macrophage populations and myofibroblastic cells, because macrophages show heterogeneous functions depending on microenvironmental factors.  相似文献   

Identification of genes expressed during spore germination of Mycosphaerella pinodes     

Hiroyuki Takahara  Kazuhiro Toyoda  Gento Tsuji  Yasuyuki Kubo  Yoshishige Inagaki  Yuki Ichinose  Tomonori Shiraishi 《Journal of General Plant Pathology》2005,71(3):190-195

Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection.  相似文献   

Computer tomography diagnosis of meningoencephalocele in a calf     

Ohba Y  Iguchi T  Hirose Y  Takasu M  Nishii N  Maeda S  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):829-831

A 24-day-old female Holstein calf had a soft, painless fluctuating swelling on the median plane in the frontal region, but did not show any clinical symptoms including neurological signs. Computer tomography (CT) distinctly showed the cyst filled with fluid and part of the encephalon. Hence, this swelling was diagnosed as meningoencephalocele, but not meningocele. The meningoencephalocele was successfully repaired surgically. Meningoencephalocele can thus be easily recognised by CT in a calf.  相似文献   

In vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (Carassius auratus langsdorfii)     

Kobayashi I  Kusakabe H  Toda H  Moritomo T  Takahashi T  Nakanishi T 《Veterinary immunology and immunopathology》2008,126(1-2):74-82

Primitive hematopoietic cells in mammalian bone marrow are purified by flow cytometry using Hoechst 33342 (Hoechst) and rhodamine-123 (Rho), because these dyes efflux activities of hematopoietic cells widely conserved in mammals. Hematopoietic stem cells (HSCs) are identified as side population (SP) cells, characterized by specific Hoechst efflux pattern in flow cytometric analysis. We previously demonstrated that SP cells from teleost body kidney (BK) had the HSC activity by a transplantation experiment using clonal ginbuna crucian carp (Carassius auratus langsdorfii). In the present study, to isolate HSCs and hematopoietic progenitor cells (HPCs) from teleosts using Hoechst and Rho, we compared the hematopoietic activity of Rho-negative (Rho(-)) cells with that of SP cells by ginbuna transplantation experiments. Rho(-) cells were clearly identified from ginbuna BK, and the majority of these cells (85%) showed a non-SP phenotype. Transplantation experiments showed that long-term repopulating activity (HSC activity) of Rho(-) cells was lower than that of SP cells, while Rho(-) cells had higher short-term repopulating activity (HPC activity) than SP cells. These results suggest that Rho(-) cells in ginbuna BK contain various stages of hematopoietic cells, while SP cells are highly enriched for HSCs, and that these dyes are useful for purification of HSCs and HPCs in teleosts.  相似文献   

Clonal structure in Ichthyobacterium seriolicida,the causative agent of bacterial haemolytic jaundice in yellowtail,Seriola quinqueradiata,inferred from molecular epidemiological analysis          下载免费PDF全文

T Matsuyama  Y Fukuda  T Sakai  N Tanimoto  M Nakanishi  Y Nakamura  T Takano  C Nakayasu 《Journal of fish diseases》2017,40(8):1065-1075

Bacterial haemolytic jaundice caused by Ichthyobacterium seriolicida has been responsible for mortality in farmed yellowtail, Seriola quinqueradiata, in western Japan since the 1980s. In this study, polymorphic analysis of I. seriolicida was performed using three molecular methods: amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST) and multiple‐locus variable‐number tandem repeat analysis (MLVA). Twenty‐eight isolates were analysed using AFLP, while 31 isolates were examined by MLST and MLVA. No polymorphisms were identified by AFLP analysis using EcoRI and MseI, or by MLST of internal fragments of eight housekeeping genes. However, MLVA revealed variation in repeat numbers of three elements, allowing separation of the isolates into 16 sequence types. The unweighted pair group method using arithmetic averages cluster analysis of the MLVA data identified four major clusters, and all isolates belonged to clonal complexes. It is likely that I. seriolicida populations share a common ancestor, which may be a recently introduced strain.  相似文献