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101.

We combined two protein-marking enzyme-linked immunosorbent assays (ELISA) with a predator gut content ELISA to monitor the movement and feeding activity of commercially-purchased Hippodamia convergens Guèrin-Mèneville (Coleoptera: Coccinellidae) under realistic field conditions during two field seasons in central Arizona. The protein-marking ELISAs were used to differentiate released H. convergens from the native beetles. Commercially purchased beetles marked with rabbit immunoglobulin G (IgG) were released into cotton fields and chicken IgG marked beetles were released into adjacent cantaloupe fields. Results showed that the total native beetle abundance in each crop was about the same size. The recovery rates after 15 days for the released beetles were less than 1.0% over all the releases, indicating that they dispersed readily from the release site. Of the recaptured beetles containing rabbit IgG (cotton), 82.2% were recovered in cotton and 11.8% moved to cantaloupe. Of those containing chicken IgG (cantaloupe), 66.5% were recovered in cantaloupe and 33.5% moved to cotton. A predator gut content ELISA was used to determine if there were differences in the frequency of predation of released versus indigenous H. convergens on the silverleaf whitefly, Bemisia argentifolii Bellows &; Perring (Homoptera: Aleyrodidae). The proportion of beetles containing whitefly antigens was always higher for the released beetles than for their native counterparts. Our results demonstrate an approach to combine protein marking and predator gut content ELISAs that allows the simultaneous comparison of feeding and intercrop movement of native and commercially-obtained biological control agents.  相似文献   
102.
Over the past decade, lambing percentages have risen in conjunction with a rise in the percentage of multiple lambs born. Multiple-born lambs are smaller than their singleton counterparts and are particularly susceptible to starvation-exposure. Any technique that can increase the birthweight or thermoregulatory capability, or both, of otherwise lightweight lambs has the potential to substantially increase survival of multiple-born lambs.

In the United Kingdom under housed conditions, shearing during pregnancy has been shown to increase both the birthweight and thermoregulatory capability of newborn lambs. However, shearing during pregnancy under pastoral conditions has failed to consistently affect the newborn lamb’s thermoregulatory capability. In contrast, under New Zealand’s pastoral conditions shearing during pregnancy has been found to increase birthweight, but results have been inconsistent in both magnitude and birth-rank specificity. Increase in feed intake by the dam, types of shearing comb used and changes in gestation length do not explain the variation observed. When studies involving shearing during pregnancy are collated it becomes apparent that there are two criteria that must be met to achieve a birthweight response. Firstly, the dam must have the potential to respond and secondly, the dam must have the means to respond.

Any increase in lamb survival through shearing during pregnancy would be predominantly through an increase in birthweight of otherwise lightweight lambs. However, in the few studies conducted to date, an increase in birthweight has not resulted in a statistically significant increase in survival. For an increase in birthweight to have a positive effect on lamb survival, lambs must be otherwise destined to be born within a birthweight range in which survival rate is below optimum (<4.0 kg), and the increase in birthweight observed must move a significant proportion of otherwise lightweight lambs into a higher range of survival rate.  相似文献   
103.
Tropical forest fragmentation threatens biodiversity, yet basic information on population responses for major groups such as plants is lacking. Hypervariable genetic markers were used to reconstruct a population-level pedigree in fragmented tropical forest for the tree Symphonia globulifera. Though seedlings occurred only in remnant forest, the pedigree showed that most seedlings had been produced by sequentially fewer adults in pasture, creating a genetic bottleneck. The pedigree also implicated shifts in the foraging of animals that disperse pollen and seed in a secondary constriction of the bottleneck. These results suggest that tropical conservation strategies should anticipate complex, cryptic responses to fragmentation.  相似文献   
104.
The routing and interconnection of optical signals through narrow channels and around sharp corners are important for large-scale all-optical circuit applications. A recent computational result suggests that photonic crystals may offer a novel way of achieving this goal by providing a mechanism for guiding light that is fundamentally different from traditional index guiding. Waveguiding in a photonic crystal and near 100 percent transmission of electromagnetic waves around sharp 90 degree corners were observed experimentally. Bending radii were made smaller than one wavelength.  相似文献   
105.
A Late Cretaceous (92 to 86 million years ago) vertebrate assemblage from the high Canadian Arctic (Axel Heiberg Island) implies that polar climates were warm (mean annual temperature exceeding 14 degreesC) rather than near freezing. The assemblage includes large (2.4 meters long) champsosaurs, which are extinct crocodilelike reptiles. Magmatism at six large igneous provinces at this time suggests that volcanic carbon dioxide emissions helped cause the global warmth.  相似文献   
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