Isolation and Application of Microsatellite DNA Markers for Pedigree Tracing of Seedlings of Red Sea Bream (Pagrus major)     

Akimasa  Hatanaka Sho-Ichi  Yamada Takashi  Sakamoto Toru  Mitsuboshi 《Journal of the World Aquaculture Society》2006,37(1):139-143

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Corrigendum to “GnRHa-accelerated spermatogenesis in the testes of underyearling golden rabbitfish, Siganus guttatus (Bloch)” [Aquaculture 257 (2006) 558 - 565]     

Toru Komatsu  Ramji K. Bhandari  Yasuhisa Kobayashi  Masaru Nakamura 《Aquaculture (Amsterdam, Netherlands)》2006,261(2):823-824

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Estimation of genetic data and breeding values of traits related to wax production in <Emphasis Type="Italic">Rhus succedanea</Emphasis> L. clones using the REML/BLUP method     

Yuichiro Hiraoka  Noritsugu Kuramoto  Mineko Ohira  Masanori Okamura  Toru Taniguchi  Yoshitake Fujisawa 《Journal of Forest Research》2011,16(6):509-517

Rhus succedanea L. is cultivated in Japan for the wax that can be extracted from its fruits. We determined genetic data for specific traits associated with wax production (fruit number per cluster, FN, fruit weight, FW, number of clusters per area, CN, and wax content, WC) in five traditional cultivars and 13 newer clones. Data were collected over 4 years and alternate bearing was observed: 2001 and 2003 were years with high yields; in 2002 and 2004 yields were poor. The restricted maximum likelihood method was used to calculate (co)variance components for analysis. Broad sense heritability was estimated and ranged from low (FN and CN) to high (FW and WC) for the traits examined. There were positive genetic correlations between FW and WC and negative ones between FN and FW throughout the 4 years. Genetic correlations between CN and the other traits were positive in good crop years and negative in bad crop years. For each assessed clone, the four traits in the 4 years were evaluated using best linear unbiased predictors (BLUP) of the clonal breeding values. The BLUP scores for both FW and WC exhibited positive correlations between pairs of years, whereas there were positive BLUP correlations for CN when the high yield and low yield years were examined separately. The traditional cultivars and the newer clones were also characterized. Some of the clones were better than the traditional cultivars with regard to wax yield and reliability of production. The trait characteristics and the future breeding are discussed.  相似文献   

First report of <Emphasis Type="Italic">Impatiens necrotic spot virus</Emphasis> infecting chrysanthemum (<Emphasis Type="Italic">Chrysanthemum morifolium</Emphasis>) in Japan     

Toru Kondo  Kazuo Yamashita  Satoru Sugiyama 《Journal of General Plant Pathology》2011,77(4):263-265

In 2009, chlorotic mottle and necrosis were observed on chrysanthemums (cv. Jimba) in Aomori Prefecture, Japan. A virus was isolated from the chrysanthemum plants by serial local-lesion transfer. The symptoms exhibited by the test plants, the particle morphology, the features of the protein and the potential for transmission by thrips were similar to those for Impatiens necrotic spot virus (INSV). The partial nucleotide sequences of the nucleocapsid protein gene and the 3′-untranslated sequence of the S RNA shared 99% identity with that of an INSV isolate. This report is the first of INSV infection of chrysanthemums in Japan.  相似文献   

Isolation of an equine coronavirus from adult horses with pyrogenic and enteric disease and its antigenic and genomic characterization in comparison with the NC99 strain     

Oue Y  Ishihara R  Edamatsu H  Morita Y  Yoshida M  Yoshima M  Hatama S  Murakami K  Kanno T 《Veterinary microbiology》2011,150(1-2):41-48

A new equine coronavirus was isolated from the feces of adult horses with pyrogenic and enteric disease. The disease outbreak was mainly observed among 2- to 4-year-old horses living in stables of a draft-horse racetrack in Japan. On comparing the isolated virus (isolate Tokachi09) with the equine coronavirus NC99 strain, no significant differences were observed in several biological properties such as hemagglutinating activity, antigenicity (in indirect immunofluorescence and neutralization tests), and one-step growth (in cell culture). The sequences of the nucleocapsid and spike genes of isolate Tokachi09 showed identical size (1341 and 4092 nucleotides, 446 and 1363 amino acids, respectively) and high similarity (98.0% and 99.0% at the nucleotides, 97.3% and 99.0% at the amino acids, respectively) to those of strain NC99. However, the isolate had a 185-nucleotide deletion from four bases after the 3'-terminal end of the spike gene, resulting in the absence of the open reading frame predicted to encode a 4.7-kDa nonstructural protein in strain NC99. These results suggest that the 4.7-kDa nonstructural protein is not essential for viral replication, at least in cell culture, and that the Japanese strain probably originated from a different lineage to the North American strain. This is the first equine coronavirus to be isolated from adult horses with pyrogenic and enteric disease.  相似文献   

Therapeutic effect of anti-TNF-α antibody and levofloxacin (LVFX) in a mouse model of enterohemorrhagic Escherichia coli O157 infection     

Emiko Isogai  Hiroshi Isogai  Kimiharu Hirose  Toru Kubota  Koichi Kimura  Nobuhiro Fujii  Shunji Hayashi  Koichi Takeshi  Keiji Oguma 《Comparative immunology, microbiology and infectious diseases》2001,24(4)

The ability of an anti-TNF-α antibody to confer protection against enterohaemorrhagic Escherichia coli (EHEC) O157 was investigated in germfree IQI mice. The use of an antibiotic levofloxacin (LVFX) alone or with the antibody was also studied. Protection included an increase in survival rate. Treatment with the anti-TNF-α antibody inhibited the histological signs associated with EHEC infection but did not prevent the colonization of EHEC or production of Shiga toxin (Stx). No clinical signs were observed and EHEC was completely eliminated in the mouse model receiving both anti-TNF-α antibody and LVFX. Anti-TNF-α antibody suppressed inflammatory cytokine response in the mouse kidney and brain by EHEC infection.  相似文献   

Effect of ovary lipid from Skipjack tuna (Katsuwonus pelamis) on brain monoamines in rats     

Ookawa K  Mochizuki K  Shida E  Suzuki T  Suzuki T  Ooba T  Matumoto T  Hokari Y  Yokogoshi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):593-598

In our previous experiments with rats, ovary lipid from Skipjack Tuna (Katsuwonus pelamis) (OLS) was shown to have a mitigating effect on anxiety and/or fear in elevated T-maze tests. This suggests that OLS has some effect on the central nervous system (CNS) of rats. Thus, we performed experiments to examine the status of CNS in rats given OLS. The effect of OLS on chronic stress was also examined at the same time. The feed for control rats used oil and fat that have the same energy percentages for n-6 and n-3 fatty acids and the same n-6/n-3 ratio as OLS. As a result, rats given OLS for 28 days had lower serotonin levels in various brain areas regardless of stress application, showing that OLS affected the serotonin nervous system. From this, it was inferred that the ability of OLS to mitigate anxiety and/or fear resulted from its action on CNS, especially the serotonin nervous system. Substances other than the essential fatty acids may have been responsible for the action of OLS on monoamines and the metabolites. The effect of OLS on CNS, especially the serotonin nervous system, suggests that OLS may suppress anxiety.  相似文献   

Zygotic gene activation and maternal factors in mammals     

Minami N  Suzuki T  Tsukamoto S 《The Journal of reproduction and development》2007,53(4):707-715

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Isolation of a Megatrypanum trypanosome from sika deer (Cervus nippon yesoensis) in Japan     

Hatama S  Shibahara T  Suzuki M  Kadota K  Uchida I  Kanno T 《Veterinary parasitology》2007,149(1-2):56-64

A trypanosome was isolated from a sika deer (Cervus nippon yesoensis) in Hokkaido, Japan, during the primary culture of sika deer renal cells. This is the first report of isolation of a Megatrypanum trypanosome from Japanese Cervidae. The trypanosome, designated TSD1, was propagated and maintained in Eagle's modified essential medium containing 20% fetal bovine serum with sika deer renal cells as feeder. The TSD1 trypanosome was morphometrically similar to Trypanosoma cervi, which is commonly isolated from American and European deer. PCR analysis with primers for 18S ribosomal DNA and nucleotide sequencing showed that TSD1 is a member of genus Trypanosoma, subgenus Megatrypanum. Phylogenetically TSD1 is closely related to T. theileri, a common trypanosome of cattle, but is distinguishable from T. theileri by some morphometrical and biological features.  相似文献   

Monitoring saline intrusion in the Ayeyarwady Delta,Myanmar, using data from the Sentinel-2 satellite mission     

Sakai  Toru  Omori  Keisuke  Oo  Aung Naing  Zaw  Yan Naung 《Paddy and Water Environment》2021,19(2):283-294

Paddy and Water Environment - Summer rice cannot be grown near the coast of the Ayeyarwady Delta, Myanmar, because of the high salinity in river water during the dry season. This means that saline...  相似文献