Interaction between the helicase domain of the tobacco mosaic virus replicase and a tobacco arginine decarboxylase     

Takumi Shimizu  Yasuyuki Yamaji  Yoshitake Ogasawara  Koji Hamada  Keitaro Sakurai  Toshihiko Kobayashi  Takato Watanabe  Tadaaki Hibi 《Journal of General Plant Pathology》2004,70(6):353-358

In a yeast two-hybrid screening test for tobacco proteins that interact with TMV replicase using the helicase (H) domain as bait, a cDNA clone was selected that encodes a polyamine biosynthetic enzyme, arginine decarboxylase (ADC). In yeast cells, the C-terminal internal region of ADC interacted with the H domain. This observation was confirmed in vitro by far-Western blotting. Inhibition of the binding between the H domain and the IRnHEL (I region and N-terminus of helicase domain) region by ADC using a yeast three-hybrid assay suggested possible interference of the heterodimerization of 126K and 183K by ADC.The nucleotide sequence data of pADCF reported in this study is available in the DDBJ/EMBL/GenBank databases under accession number AB110952  相似文献   

Development of spermatogenic function in the sex maturation process in male cats     

Tsutsui T  Kuwabara S  Kuwabara K  Kugota Y  Kinjo T  Hori T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1125-1127

The spermatogenic function and plasma testosterone (T) level in the sex maturation process were investigated as to 180 mixed breed cats ranging from 4 months to 2 years in age to be castrated. Testis/epididymis weights reached a peak at 10 and 8 to 9 months of age, respectively. In the testis, sperm appeared at 5 months of age. At 7 months of age, sperm were observed in 96.2% of the cats. In the tail of the epididymis, sperm appeared in 46.9% of the cats at 6 months of age and in all cats at 8 or more months of age. Furthermore, the mean plasma T level rapidly increased at 8 months of age, and reached a peak (2.64 +/- 0.68 (SE) ng/ml) at 10 months of age. Three of 180 cats (1.67%) had unilateral cryptorchidism. These results suggest that the spermatogenic function in male cats becomes mature at 8 to 10 months of age.  相似文献   

Relationship between the sperm count and the fertilization rate of ova ovulated from the contralateral ovary in intrauterine horn insemination in cats     

Tsutsui T  Nagakubo K  Hori T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1143-1145

Unilateral intrauterine horn insemination (UIUI) was carried out in cats, and we investigated the fertilization rate of ova ovulated from the contralateral ovary. Various numbers of sperm were used to inseminate the uterine horn on the side where ovulation was inhibited. The rates of conception were 1/11 (9.1%), 2/11 (18.2%), and 5/7 (71.4%) in the 2 x 10(6), 4 x 10(6), and 8 x 10 (6) groups, respectively. Furthermore, the fertilization rate was 70.7% in the 8 x 10(6) group. Thus, ova ovulated from the contralateral ovary were not fertilized or the fertilization rate was low in some cats even when UIUI was performed with a large number of sperm.  相似文献   

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland     

Yamaji D  Kimura K  Watanabe A  Kon Y  Iwanaga T  Soliman MM  Ahmed MM  Saito M 《Domestic animal endocrinology》2006,30(3):239-246

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4152 bp open reading frame coding a 1384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.  相似文献   

Estimating the origins of adult chum salmon <Emphasis Type="Italic">Oncorhynchus keta</Emphasis> in the Okhotsk and Sea of Japan regions using discriminant analysis of scale characteristics     

Toshihiko Saito 《Fisheries Science》2017,83(3):383-400

Linear discriminant analysis (LDA) based on scale patterns was used to develop a methodology of estimating regional origins of chum salmon. Age-4 fish were sampled in 2004–2006 from 12 river stocks of the Okhotsk and Sea of Japan (SJ) regions from Hokkaido to Honshu. The scale radius at the first annulus of each fish was separated into i intervals and the radius of each interval was divided by the number of scale circuli within the interval to quantify scale patterns. The i variables and five other morphometric measurements were used in a stepwise LDA to classify the following regional groups: Hokkaido and Honshu (I), Okhotsk and SJ (II), Okhotsk, Hokkaido SJ and Honshu SJ (III). Percentages of correctly classified fish (hit rates) improved with increased i but tended to be close to asymptotic values in all cases. Hit rates for each river stock in case (I) ranged from 74.3% to 100% (mean 97.2%), estimated by direct maximum likelihood methods using predictor variable sets from the best models for LDAs. Hit rates were lower in cases (II) and (III). This study demonstrated that scale patterns are useful for classifying the origins of chum salmon, at least between Hokkaido and Honshu.  相似文献   

Growth,sex ratio,and maturation rate with age in the blackspot tuskfish <Emphasis Type="Italic">Choerodon schoenleinii</Emphasis> in waters off Okinawa Island,southwestern Japan     

Akihiko Ebisawa  Kiyoaki Kanashiro  Toshihiko Kiyan 《Fisheries Science》2010,76(4):577-583

The growth, sex ratio with age, and age at sexual maturation were determined based on sectioned otoliths in 257 specimens of the blackspot tuskfish Choerodon schoenleinii collected in waters off Ryukyu Island. Opaque rings observed by reflected light in the sectioned otoliths were found to form once a year from January to July. The three growth parameters of the von Bertalanffy growth equation were L_∞ = 68.1 (cm), k = 0.263, and t ₀ = −0.023 (year). The age at which the sex ratio reached 50% by sexual transition was about 6.15 years, and the age at which 50% of females were sexually mature was approximately 2 years. The oldest specimen among the samples was 17 years old.  相似文献   

Release of Bound Lipids in Cereal Starches Upon Hydrolysis by Glucoamylase     

Kanefumi Kitahara  Takako Tanaka  Toshihiko Suganuma  Tomonori Nagahama 《Cereal Chemistry》1997,74(1):1-6

The raw starch granules from corn, rice, and wheat were hydrolyzed by practically pure glucoamylase (Rhizopus niveus). The bound lipids remaining in the residual starches were investigated, of which the major components of the lipids, free fatty acids (FFA) in corn starch, FFA and phospholipids (PL) in rice starch, and PL in wheat starch were determined. In each case, the bound FFA and PL were decreased to some extent during the initial stage of hydrolysis. During the later stages, the FFA continued to gradually decrease, while the level of PL stabilized. It was interesting that some of the bound lipids were released from the granules upon glucoamylase hydrolysis, differing from the model amylose-lipid complexes. Furthermore, the structures of the residual starches were investigated. The blue value and λ_max of the starches were increased by partial hydrolysis of the starch granules using practically pure glucoamylase. Two gel-permeation chromatography analyses revealed that the relative amount of amylose fraction was increased by glucoamylase hydrolysis, and also that the increments were reduced by the defatting of bound lipids. The results suggest that the increase in amylose fraction is attributable to the existence of bound lipids in the granules.  相似文献   

Duration of estrus induced after GnRH‐PGF2α protocol in dairy heifer     

Chikako YOSHIDA  Muhammad YUSUF  Toshihiko NAKAO 《Animal Science Journal》2009,80(6):649-654

Estrous expressions in dairy cows have been shortened and weakened. Dairy heifers, on the other hand, may not have had such changes in estrous signs as observed in cows, since they have less stresses than cows. The aim of this study was to describe the duration of estrus in a herd of dairy heifers. A total of 56 Holstein Friesian heifers estrus was synchronized using two different hormonal protocols. They were checked for primary and secondary estrous signs with the help of heat detection devices for 48 h at an interval of 4 h starting at 16.00 hour, one day after PGF_2α treatment. Onset and end of standing estrus during 48 h observation period was recorded in 35 of the 44 heifers coming into estrus within 5 days after PGF_2α treatment during the observation period. The duration of standing estrus on the average (±SD) was 9.7 ± 5.3 h. Percentage of heifers with standing estrus longer than 12 h was 40%, and 53% showed standing estrus only for 4–8 h. It is indicated that duration of estrus in dairy heifers has been shortened recently.  相似文献   

The beneficial effects of horse trekking on autonomic nervous activity in experienced rider with no disability          下载免费PDF全文

Akihiro Matsuura  Haruhiro Maruta  Tomohiko Iwatake  Takashi Kumagai  Toshihiko Nakanowatari  Koichi Hodate 《Animal Science Journal》2017,88(1):173-179

The aim of this study was to determine whether autonomic nervous activity of a rider with no disability was altered by one practical and applicable horse trekking (HT) exercise. Changes in autonomic nervous activity were analyzed by heart rate variability (HRV). Twenty‐three participants with no disability rode horses along a predetermined HT course at trotting and walking for 60 min. HRV was sampled at 60 min before and immediately, following 60 min, and 120 min after HT. As a control, the same measurements were performed for 22 age‐matched participants during their rest. Only in the HT group, the value of normalized unit in high frequency component (HF nu), an index of parasympathetic nervous activity, was higher at 120 min after treatment than before HT (P < 0.05). The low / high frequency ratio (LF / HF), believed to reflect sympathetic nervous activity, was lower in the HT group than those in the control group at 60 min (P < 0.05) and 120 min after treatment (P < 0.01). These findings suggest that a single HT shifted the autonomic nervous balance of a rider toward parasympathetic dominance. The results obtained by the present study could accelerate the use of horses for human health.  相似文献   

Traumatic Pneumothorax Associated With Penetrating Neck Injury Caused by a Stingray: A Case Report     

Toshinao Suzuki  Toshihiko Takada  Junmei Fudoji 《The Forestry Chronicle》2017,28(2):119-121

  相似文献