Changes in the plasma testosterone level and testicular superoxide dismutase activity of 5 azoospermic beagles after GnRH analogue injections     

Kawakami E  Kuroda D  Hori T  Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):561-562

The peripheral blood plasma testosterone (T) levels and superoxide dismutase (SOD) activity were measured in 5 azoospermic (AZ-) beagles. The mean values in the AZ-dogs were significantly lower than in 7 control beagles (P<0.001). Subcutaneous injections of 1 mug/kg GnRH analogue three times weekly in the AZ-dogs induced significant increases in mean T level and SOD activity (P<0.05) and improvement in spermatogenesis. Thus, spermatogenic function in the dog appears to be maintained by T and normal SOD activity in the testis.  相似文献   

Severe hypoplasia of the omasal laminae in a Japanese Black steer with chronic bloat--a case report     

Takagi M  Mukai S  Fushimi Y  Matsushita K  Miyoshi N  Yasuda N  Kitajima H  Takamure S  Matsushita T  Kitamura N  Deguchi E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1281-1286

An 11-month-old Japanese Black steer with chronic bloat underwent clinical and histological analyses. During the observation period, it showed normal appetite and fecal volume but persistent chronic bloat symptoms. Compared to controls, the steer's feces contained undigested large straws. Necropsy revealed normal rumen, reticulum, and abomasum but a small omasum. The rumen, reticulum, and abomasum mucosa was normal, with well-developed ruminal papillae. However, severe hypoplasia of the omasal laminae was observed along with hypoplasia reticular groove and ruminoreticular fold. The contents of the reticulum, omasum, and abomasums comprised undigested large sized hay particles. The omasum papillae showed no pathological abnormalities. This is a rare case of a steer with chronic bloat probably caused by severe hypoplasia of the omasal laminae.  相似文献   

Peripartum changes in plasma estrone sulfate and estradiol-17beta profiles associated with and without the retention of fetal membranes in holstein-friesian cattle     

Shah KD  Nakao T  Kubota H  Maeda T 《The Journal of reproduction and development》2007,53(2):279-288

The aim of this study was to evaluate the changes in plasma concentrations of estrone sulfate (E(1)S) and estradiol-17beta (E(2)beta) during the peripartum period (from day 10 prepartum to day 1 postpartum) associated with and without retention of fetal membranes (RFM) in Holstein-Friesian cattle (n=42). Plasma samples were analyzed for E(1)S and E(2)beta by ELISA. All parturitions were spontaneous and normal. Of 38 cattle delivering singletons, 29 had no RFM (singleton-normal group) and nine had RFM for more than 12 h (singleton-RFM group). Four cows gave birth to twins, and each twin had its own fetal membrane (FM). Two twinning cows expelled both FMs normally within 12 h (twin-normal group). In the remaining 2 twinning cows (twin-RFM group), the FM was expelled normally for one twin (first), while the FM of the other (second) was retained. There were no significant differences in the E(1)S concentrations or their increments from the concentrations on the preceding day between the normal and RFM groups of singleton cows on any peripartum day. The mean plasma E(2)beta concentrations on each day from day 10 to day 3 prepartum were significantly lower (P<0.05) in the singleton-RFM group compared with the singleton-normal group; however, on days 2 and 1 prepartum, the increments in the E(2)beta concentrations from the concentrations on the preceding days were significantly higher (P<0.05) in the singleton-RFM group than in the singleton-normal group. Thus, the plasma E(1)S concentrations just before parturition may not be associated with RFM. In the cows with RFM, the lower plasma E(2)beta concentrations that were found prior to day 2 prepartum may have been associated with immature placentomes, and the rapid rise in plasma E(2) beta within 1 to 2 days prior to calving may have produced asynchrony of placental and/or fetal maturation in relation to calving, thus resulting in RFM.  相似文献   

Cesium radioactivity in peripheral blood is linearly correlated to that in skeletal muscle: Analyses of cattle within the evacuation zone of the Fukushima Daiichi Nuclear Power Plant          下载免费PDF全文

Tomokazu Fukuda  Yasushi Kino  Yasuyuki Abe  Hideaki Yamashiro  Jin Kobayashi  Yoshinaka Shimizu  Atsushi Takahashi  Toshihiko Suzuki  Mirei Chiba  Shintaro Takahashi  Kazuya Inoue  Yoshikazu Kuwahara  Motoko Morimoto  Hisashi Shinoda  Masahiro Hiji  Tsutomu Sekine  Manabu Fukumoto  Emiko Isogai 《Animal Science Journal》2015,86(1):120-124

The accident at the Fukushima Daiichi Nuclear Power Plant (FNPP) released a large amount of radioactive substances into the environment. Furthermore, beef contaminated with radioactive cesium above the 500 Bq/kg safety standard was circulated in the food chain in 2011. Japanese consumers remain concerned about the safety of radioactively contaminated food. In our previous study, we detected a linear correlation between radioactive cesium (¹³⁷Cs) activity in blood and muscle around 500 to 2500 Bq/kg in cattle. However, it was unclear whether the correlation was maintained at a lower radioactivity close to the current safety standard of 100 Bq/kg. In this study, we evaluated 17 cattle in the FNPP evacuation zone that had a ¹³⁷Cs blood level less than 10 Bq/kg. The results showed a linear correlation between blood ¹³⁷Cs and muscle ¹³⁷Cs (Y = 28.0X, R² = 0.590) at low radioactivity concentration, indicating that cesium radioactivity in the muscle can be estimated from blood radioactivity. This technique would be useful in detecting high‐risk cattle before they enter the market, and will contribute to food safety.  相似文献   

Tech.Sight. Capillary electrophoresis. Ultra-high resolution separation comes of age     

Linhardt RJ  Toida T 《Science (New York, N.Y.)》2002,298(5597):1441-1442

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Systematic assessment of flood mitigation in a tank irrigated paddy fields area     

Koichi Unami  Toshihiko Kawachi 《Paddy and Water Environment》2005,3(4):191-199

Flood mitigation in irrigation tanks and paddy fields is their favorable aspect though its practical effect is not known very well. A dynamic and systematic approach is presented to assess flood mitigation in a tank irrigated paddy fields area in the worst case where no static buffer function is expected. Based on the linear control theory, transfer function models for runoff process in catchments are identified. Hydraulic models are developed to represent flood dynamics in irrigation tanks, paddy fields, and drainage channels. These models are integrated as an ordinary differential equations system. Then, using the perturbed linear system, flood mitigation in each component of the system is examined in terms of frequency response. An application example demonstrates that a tank irrigated paddy fields area has a significant flood mitigation effect as a low-pass filter. This method has the advantage of assessing flood mitigation even in the case of an increase in the total runoff ratio.  相似文献   

Effects of Treatment for Anestrus in Water Buffaloes with PGF2α and GnRH in Comparison with Vitamin-Mineral Supplement,and Some Factors Influencing Treatment Effects     

Bhuminand DEVKOTA  Toshihiko NAKAO  Kosaku KOBAYASHI  Hiroshi SATO  Shyam Kishor SAH  Dinesh Kumar SINGH  Ishwori Prasad DHAKAL  Norio YAMAGISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1623-1627

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Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice     

Makoto Shirai  Shingo Arakawa  Hiroaki Miida  Takuya Matsuyama  Junzo Kinoshita  Toshihiko Makino  Kiyonori Kai  Munehiro Teranishi 《Journal of toxicologic pathology》2013,26(2):175-186

To assess modification of thioacetamide-induced hepatotoxicity in mice fed a high-fat diet, male C57BL/6J mice were fed a normal rodent diet or a high-fat diet for 8 weeks and then treated once intraperitoneally with thioacetamide at 50 mg/kg body weight. At 24 and 48 hours after administration, massive centrilobular hepatocellular necrosis was observed in mice fed the normal rodent diet, while the necrosis was less severe in mice fed the high-fat diet. In contrast, severe swelling of hepatocytes was observed in mice fed the high-fat diet. In addition, mice fed the high-fat diet displayed more than a 4-fold higher number of BrdU-positive hepatocytes compared with mice fed the normal rodent diet at 48 hours after thioacetamide treatment. To clarify the mechanisms by which the hepatic necrosis was attenuated, we investigated exposure to thioacetamide and one of its metabolites, the expression of CYP2E1, which converts thioacetamide to reactive metabolites, and the content of glutathione S-transferases in the liver. However, the reduced hepatocellular necrosis noted in mice fed the high-fat diet could not be explained by the differences in exposure to thioacetamide or thioacetamide sulfoxide or by differences in the expression of drug-metabolizing enzymes. On the other hand, at 8 hours after thioacetamide administration, hepatic total glutathione in mice fed the high-fat diet was significantly lower than that in mice fed the normal diet. Hence, decreased hepatic glutathione amount is a candidate for the mechanism of the attenuated necrosis. In conclusion, this study revealed that thioacetamide-induced hepatic necrosis was attenuated in mice fed the high-fat diet.  相似文献   

Method for estimating maximum permissible load weight for Japanese native horses using accelerometer‐based gait analysis     

Akihiro MATSUURA  Mami IRIMAJIRI  Kunihiro MATSUZAKI  Yuko HIRAGURI  Toshihiko NAKANOWATARI  Atusi YAMAZAKI  Koichi HODATE 《Animal Science Journal》2013,84(1):75-81

The aim of this study was to establish a method for estimating loading capacity for Japanese native horses by gait analysis using an accelerometer. Six mares of Japanese native horses were used. The acceleration of each horse was recorded during walking and trotting along a straight course at a sampling frequency of 200 Hz. Each horse performed 12 tests: one test with a loaded weight of 80 kg (First 80 kg) followed by 10 tests with random loaded weights between 85 kg and 130 kg and a final test with a loaded weight of 80 kg again. The time series of acceleration was subjected to fast Fourier transformation, and the autocorrelation coefficient was calculated. The first two peaks of the autocorrelation were defined as symmetry and regularity of the gait. At trot, symmetries in the 100, 110, and 125 kg tests were significantly lower than that in First 80 kg (P < 0.05, by analysis of covariance and Sidak's test). These results imply that the maximum permissible load weight is less than 100 kg, which is 29% of the body weight of Japanese native horses. Our method is a widely applicable and welfare‐friendly method for estimating maximum permissible load weights of horses.  相似文献   

Bite Wounds Caused by a Wild Boar: A Case Report     

Hiroki Nagasawa  Kazuhiko Omori  Hiroyuki Maeda  Ikuto Takeuchi  Suguru Kato  Takashi Iso  Kei Jitsuiki  Toshihiko Yoshizawa  Kouhei Ishikawa  Hiromichi Ohsaka  Youichi Yanagawa 《The Forestry Chronicle》2017,28(4):313-317

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