侵蚀黑土玉米和大豆根部伴生细菌群落结构分析     

喻江  范国权  IKENAGA Makoto  SAKAI Masao  王光华  刘晓冰  于镇华 《大豆科学》2021,40(2):232-240

为分析东北侵蚀黑土中大豆和玉米根部伴生细菌群落结构多样性,促进土壤侵蚀过程中其根部伴生细菌群落结构响应规律的研究,本研究结合LNA-PCR技术和高通量测序方法,模拟东北黑土侵蚀土壤,分析玉米播种期和抽穗期、大豆开花期根部细菌群落结构多样性和差异.结果表明:土壤表层剥离后大豆根部细菌群落多样性降低,而玉米根部细菌群落多样...  相似文献   

Mid-to-caudal partial hemipelvectomy with limb preservation for ischial tumor in a dog     

Yoshifumi ENDO  Toshikazu SAKAI  Sho FUKUI  Ai HORI  Ryosuke ECHIGO  Satoru MATSUNAGA  Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):218

A 10-year-old spayed female Golden Retriever was referred for hindlimb lameness. A firm mass was palpated over the right caudal pelvis. Computed tomography revealed an osteolytic bone region and an associated periosteal reaction in the ischium, including the acetabulum. The histological diagnosis was sarcoma of unknown origin. A mid-to-caudal partial hemipelvectomy was performed to remove the mass. Femoral head and neck osteotomy was performed to allow hindlimb preservation. Following surgery, the dog regained satisfactory hindlimb use over time and was alive for 821 days with no recurrence or metastatic disease. This report indicates that mid-to-caudal partial hemipelvectomy with femoral head and neck osteotomy is technically feasible and allows for tumor control with preservation of the hindlimb and its function.  相似文献   

Seasonal changes in icosapentaenoic acid and docosahexaenoic acid contents in muscle lipids of Hokkai prawn Pandalus kessleri     

Takamasa  KASAI  Hiroshige  SAKAI 《Fisheries Science》2004,70(3):527-529

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Sensitivity of FCV to recombinant feline interferon (rFeIFN)   总被引：1，自引：0，他引：1  

Ohe K  Takahashi T  Hara D  Hara M 《Veterinary research communications》2008,32(2):167-174

Feline calicivirus cause feline respiratory diseases, and inactivated and attenuated vaccines are available for its prevention. Moreover, the presence of vaccine breakdown strains (VBS) is problematic. In Japan, feline recombinant interferon (rFeIFN) has been used for its treatment. However the method of compare with each strains has not established. To examine the relationship between the breakdown vaccine strain and rFeIFN sensitivity, the sensitivity of 47 field isolates to rFeIFN was determined. The Log PDD₅₀ values were normally distributed within the range 1.1–3.7, with a mean value of 2.3 ± 0.64. Since 68.3% of the PDD values fell in the range of the mean ± standard deviation, the values in the range 1.7–2.9, the lower values, and the higher values were defined as representing moderate, low, and high sensitivity, respectively. Among the 15 vaccine breakdown strains, strain Fukuoka9 showed a low sensitivity, but strains ML89, T58, and N74 were highly sensitive, showing no association with vaccine breakdown. The amino acid sequence changes specific to the low rFeIFN-sensitive Fukuoka-9 strain were found, suggesting that these sites are involved in rFeIFN sensitivity.  相似文献   

A single injection of periostin decreases cardiac voltage-gated Na+ channel in rat ventricles     

Keisuke IMOTO  Yuho SAKAI  Muneyoshi OKADA  Kosuke OTANI  Hideyuki YAMAWAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):997

Changes in electrophysiological properties, such as ion channel expression and activity, are closely related to arrhythmogenesis during heart failure (HF). However, a causative factor for the electrical remodeling in HF has not been determined. Periostin (POSTN), a matricellular protein, is increased in heart tissues of patients with HF. In the present study, we investigated whether a single injection of POSTN affects the electrophysiological properties in rat ventricles. After male Wistar rats were intravenously injected with recombinant rat POSTN (64 µg/kg, 24 hr), electrocardiogram (ECG) was recorded. Whole-cell patch clamp was performed to measure action potential (AP) and Na⁺ current (I_Na) in isolated ventricular myocytes. Protein expression of cardiac voltage-gated Na⁺ channel (Na_V1.5) in isolated ventricles was examined by Western blotting. In ECG, POSTN-injection significantly increased RS height. POSTN-injection significantly delayed time to peak in AP and decreased I_Na in the isolated ventricular myocytes. POSTN-injection decreased Na_V1.5 expression in the isolated ventricles. It was confirmed that POSTN (1 µg/ml, 24 hr) decreased I_Na and Na_V1.5 protein expression in neonatal rat ventricular myocytes. This study for the first time demonstrated that a single injection of POSTN in rats decreased I_Na by suppressing Na_V1.5 expression in the ventricular myocytes, which was accompanied by a prolongation of time to peak in AP and an increase of RS height in ECG.  相似文献   

Implantation of Tetrapod-Shaped Granular Artificial Bones or β-Tricalcium Phosphate Granules in a Canine Large Bone-Defect Model     

Sungjin CHOI  I-Li LIU  Kenichi YAMAMOTO  Muneki HONNAMI  Takamasa SAKAI  Shinsuke OHBA  Ryosuke ECHIGO  Shigeki SUZUKI  Ryouhei NISHIMURA  Ung-il CHUNG  Nobuo SASAKI  Manabu MOCHIZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(2):229-235

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Molecular loops in the galactic center: evidence for magnetic flotation     

Fukui Y  Yamamoto H  Fujishita M  Kudo N  Torii K  Nozawa S  Takahashi K  Matsumoto R  Machida M  Kawamura A  Yonekura Y  Mizuno N  Onishi T  Mizuno A 《Science (New York, N.Y.)》2006,314(5796):106-109

The central few hundred parsecs of the Milky Way host a massive black hole and exhibit very violent gas motion and high temperatures in molecular gas. The origin of these properties has been a mystery for the past four decades. Wide-field imaging of the (12)CO (rotational quantum number J = 1 to 0) 2.6-millimeter spectrum has revealed huge loops of dense molecular gas with strong velocity dispersions in the galactic center. We present a magnetic flotation model to explain that the formation of the loops is due to magnetic buoyancy caused by the Parker instability. The model has the potential to offer a coherent explanation for the origin of the violent motion and extensive heating of the molecular gas in the galactic center.  相似文献   

Characterization of L-amino acid oxidase and antimicrobial activity of aplysianin A, a sea hare-derived antitumor-antimicrobial protein     

Mitsuru  JIMBO  Fumie  NAKANISHI  Ryuichi  SAKAI  Koji  MURAMOTO  Hisao  KAMIYA 《Fisheries Science》2003,69(6):1240-1246

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Influence of sodium chloride on superoxide dismutase activity and lipid peroxidation in refrigerated and frozen yellowtail meat     

Tadashi  SAKAI  Nobuhiko  UEKI  Deepthi  MS MUNASINGHE 《Fisheries Science》2003,69(5):1060-1064

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Internal migration of sodium chloride, sorbitol and moisture in fish meat during soaking and cold preservation     

KATSUHISA  TSURUHASHI  TOORU  OOIZUMI  YOSHIAKI  AKAHANE  TOORU  SAKAI 《Fisheries Science》2003,69(4):836-841

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