Genetic diversity ofKandelia candel andBruguiera gymnorrhiza in the Southwest Islands, Japan     

Toshikazu Takeuchi  Takashi Sugaya  Ayako Kanazashi  Hiroshi Yoshimaru  Masaki Katsuta 《Journal of Forest Research》2001,6(3):157-162

The genetic diversity of the mangrove tree speciesKandelia candel andBruguiera gymnorrhiza in the Southwest Islands of Japan was investigated. These islands are located at the periphery of the distribution area of the two species, and their populations are relatively small. Allozyme analyses of 17 loci inK. candel and 13 loci inB. gynmorrhiza revealed very little genetic variation in both species. At the species level, the proportion of polymorphic loci was 4.2%, the average effective number of alleles per locus was 1.02, and the average expected heterozygosity was 0.012 inK. candel. The corresponding figures forB. gymnorrhiza were 9.0%, 1.06 and 0.035, respectively. However, the coefficients of genetic differentiation among the populations were high (G _ST=0.165 forK. candel and 0.253 forB. gymnorrhiza). The genetic variation and habitat area ofK. candel on Amami Island is greater than on the islands Okinawa and Iriomote. The genetic variation and habitat area ofB. gymnorrhiza is greater on Iriomote Island than on the islands Okinawa and Amami. The level of genetic variation in both of the species might be related to their population sizes.  相似文献   

Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum     

Masahito WATANABE  Mirina KOBAYASHI  Masaki NAGAYA  Hitomi MATSUNARI  Kazuaki NAKANO  Miki MAEHARA  Gota HAYASHIDA  Shuko TAKAYANAGI  Rieko SAKAI  Kazuhiro UMEYAMA  Nobuyuki WATANABE  Masafumi ONODERA  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2015,61(3):169-177

Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum.  相似文献   

Mixed germ cell-sex cord-stromal tumor with a concurrent interstitial cell tumor in a ferret     

Saki INOUE  Kayoko YONEMARU  Tokuma YANAI  Hiroki SAKAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):225-228

A 5-year-old male ferret presented with an enlarged canalicular testis in the left inguinal region. Microscopically, the enlarged testis consisted of a diffuse intimately admixed proliferation of c-kit-positive germ cell-like and Wilms tumor-1 protein-positive Sertoli cell-like components, but no Call-Exner body was detected. In addition, the compact proliferation of steroidogenic acute regulatory protein-intense positive interstitial cells was identified in a separate peripheral area of the mass. Based on histopathological and immunohistochemical findings, the tumor was diagnosed as a mixed germ cell-sex cord-stromal tumor with a concurrent interstitial cell tumor.  相似文献   

Spatial distribution analysis of the North Pacific spiny dogfish,Squalus suckleyi,in the North Pacific using generalized additive models          下载免费PDF全文

Toshikazu Yano  Seiji Ohshimo  Minoru Kanaiwa  Tsutomu Hattori  Masa‐aki Fukuwaka  Toru Nagasawa  Sho Tanaka 《Fisheries Oceanography》2017,26(6):668-679

The North Pacific spiny dogfish (SPD), Squalus suckleyi, is a commercially exploited shark species that plays an important role in the ecosystem. To elucidate the distribution of the SPD in the North Pacific and to evaluate the effects of sea surface temperature (SST) and prey availability on its distribution, we estimated the probability of SPD presence using a generalized additive model with a binomial error distribution from SPD presence/absence data on 14,824 operations in fishery‐independent gillnet surveys between 1972 and 2011. The habitat model was structured in the east and west to reflect differences in the North Pacific oceanic environments. In the east, a higher probability of SPD presence was identified along the coast from the eastern Gulf of Alaska to Queen Charlotte Sound. In the west, it was identified around northern Japan. The estimated distribution was continuous between the two areas, whereas the probability of SPD presence was relatively low. Although the probability of SPD presence was higher at SSTs between 6°C and 12°C, the SST at the peak probability of SPD presence differed between the west and east. The prey species, Japanese sardine, Sardinops melanostictus, and walleye pollock, Gadus chalcogrammus, in the west and boreal clubhook squid, Onychoteuthis borealijaponica, in the east significantly affected the probability of SPD presence, which was higher if the prey species co‐existed with SPD. Therefore, SPD might adapt their distribution to that of available prey species. SPD stock assessment and management in these two important areas are required for its sustainable utilization.  相似文献   

Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide     

Nana TANAKA  Toshitaka KANEI  Munetaka IWATA  Mifumi KAWABE  Hiroaki KAMISHINA  Mami MURAKAMI  Hiroki SAKAI  Sadatoshi MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1559

In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. We herein quantitatively analyzed CTL infiltration by immunohistochemical staining with granzyme B and CD3 antibodies using paraffin sections of a dog with EM associated with zonisamide. The present results indicated approximately 70% of cells at the border between the epidermis and dermis consisted of CTLs. Detection of granzyme B and CD3 using paraffin sections employed in this study can be a clinically applicable method for detecting CTLs.  相似文献   

Food-entrainable circadian oscillator in goldfish: multiple daily feeding times and food-anticipatory activity     

Sunuma  Toshikazu  Amano  Masafumi  Iigo  Masayuki  Yamamori  Kunio 《Fisheries Science》2009,75(1):207-214

The existence of a food-entrainable circadian oscillator (FEO) was examined in goldfish Carassius auratus. Single goldfish were exposed to light–dark (LD) 12:12 (lights on 06:00–18:00 h) and fed at 12:00 h (phase I). The photoperiod regime was shifted to constant light (LL) (phase II). The fish were then fed at 06:00 or at 18:00 h (phase III), and all fish were fasted (phase IV). In both groups, three out of eight fish exhibited food-anticipatory activity (FAA) in phases II and III, the free-running period (τ) of locomotor activity was approximately 24.0 h, and circadian rhythms of locomotor activity started to free-run from the previous FAA in phase IV, indicating the existence of an FEO. Next, single goldfish were exposed to LD 12:12 and were fed twice daily at 12:00 and 00:00 h (phase I). The photoperiod regime was shifted to LL (phase II), and the fish were fasted (phase III). In phase II, FAA was observed in three out of six fish for both feeding times. In phase III, a circadian rhythm of locomotor activity was observed in five fish, and circadian rhythms started to free-run from the previous feeding time (either 12:00 or 00:00 h) in three fish. These results suggest that individual goldfish can remember two daily feeding times.  相似文献   

Isolation and sequence analysis of the promoter and an allelic sequence of the iron-sulfur protein subunit gene from the white-rot fungusPleurotus ostreatus     

Toshikazu Irie  Yoichi Honda  Takuro Matsuyama  Takashi Watanabe  Masaaki Kuwahara 《Journal of Wood Science》1998,44(6):491-494

We have isolated a structural gene ofsdil, which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (EC 1.3.99.1), from a white-rot basidiomycete,Pleurotus ostreatus. Here we report isolation of the promoter region ofsdil and an allelic sequence encoding the second-type cDNA fragment isolated in the former experiments. The nucleotide sequence analysis of the promoter region revealed the existence of putative CAAT and TATA boxes, which permits us to develop an expression system in this species. The Southern blot analysis and the restriction fragment length polymorphism assay using monokaryotic strains demonstrated that no family genes tosdil exist in the haploid genome ofP. ostreatus. Moreover, a genetic analysis to detect a linkage between thesdil genotypes and flutolanil resistance in the mutantP. ostreatus strains was also developed.  相似文献   

Notes on the Occurrence of Pathogenic Races of <Emphasis Type="Italic">Xanthomonas oryzae </Emphasis>pv. <Emphasis Type="Italic">oryzae </Emphasis>Found in Hiroshima Prefecture in 1999     

Takashi OKU  Kotaro SATO  Masaki KURAO  Shohei MATSUURA  Yasufumi SAKAI  Takao TSUCHIYA 《Journal of General Plant Pathology》2000,66(4):332-334

The pathogenic race of 59 cultures of Xanthomonas oryzae pv. oryzae, a pathogen of bacterial leaf blight of rice, isolated from six locations in the inland mountainous area of Hiroshima Prefecture in 1999, were determined by a set of traditional differentials. Four races—I, II, V and VII—were found across the area; however, we noticed the composition of the races as well as the dominant race in each location different. All races were avirulent on differential cultivar Te-tep. Races V and VII were new to Hiroshima. The rice cultivars infected with bacterial leaf blight in Hiroshima are thought to be grouped into the Kinmaze group, which does not have any resistance genes. Apparently, a variety of races occurred unexpectedly on the cultivars contrary to stabilizing selection theory. Received 25 February 2000/ Accepted in revised form 13 July 2000  相似文献   

Dystrophin-deficient muscular dystrophy in a Toy Poodle with a single base pair insertion in exon 45 of the Duchenne muscular dystrophy gene     

Kosei SAKAI  Tomoki MOTEGI  James Ken CHAMBERS  Kazuyuki UCHIDA  Hidetaka NISHIDA  Shunsuke SHIMAMURA  Hiroyuki TANI  Terumasa SHIMADA  Masaru FURUYA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):502

A 10-month-old, intact male Toy Poodle was referred for a postural abnormality. Blood biochemical tests revealed a marked increase in plasma creatine phosphokinase (CPK) concentration. The isoenzyme test showed that 99% of serum CPK consisted of CPK-MM. Histopathological evaluation of muscle biopsy samples confirmed scattered degeneration and necrosis of myofibers. Immunohistochemistry for dystrophin showed an absence of staining in muscle cells. Based on these findings, the dog was diagnosed with dystrophin-deficient muscular dystrophy. Whole genome sequencing using genomic DNA extracted from blood revealed a single base pair insertion in exon 45 of the Duchenne muscular dystrophy (DMD) gene. This is the first report on muscular dystrophy in Toy Poodles and identified a novel mutation in the DMD gene.  相似文献   

Carbon stock in litter, deadwood and soil in Japan's forest sector and its comparison with carbon stock in agricultural soils   总被引：1，自引：0，他引：1  

Masamichi TAKAHASHI  Shigehiro ISHIZUKA  Shin UGAWA  Yoshimi SAKAI  Hisao SAKAI  Kenji ONO  Shoji HASHIMOTO  Yojiro MATSUURA  Kazuhito MORISADA 《Soil Science and Plant Nutrition》2010,56(1):19-30

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