排序方式： 共有57条查询结果，搜索用时 15 毫秒
41.
Minoru Sato Zhi Hua Tao Kazuhiro Shiozaki Toshiki Nakano Toshiyasu Yamaguchi Takehiko Yokoyama Nobuhiro Kan-No Eizoh Nagahisa 《Fisheries Science》2006,72(4):889-892
ABSTRACT: The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly's reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g). 相似文献
42.
Somaclonal and chromosomal effects of genotype, ploidy and culture duration in Asparagus officinalis L. 总被引：1，自引：0，他引：1
Chromosome and morphological variations of embryogenic calli-derived plants of gynogenic haploid, diploid, triploid and tetraploid
asparagus (Asparagus officinalis L.) were investigated. Artificial tetraploids were produced using colchicine treatment of
seeds of diploid cultivar, ‘Poultom’. ‘Haidel’ (2X) was crossed with the artificial tetraploids, from which one gynogenic
haploid, one diploid, 6 triploid, 3 mixoploids were obtained. Embryogenic calli were first obtained from crown buds, subsequently
induced to form somatic embryos, and after 30 days, induced to germinate. Chromosome variation in embryogenic calli-derived
plants increased with increasing duration of subculture, particularly when low ploidy levels of plants such as haploid and
diploid were used as explants. Approximately 80% of haploid-derived plants showed morphological variations such as dwarfness
and abnormal morphological characteristics, although no differences were observed in cladodes and stem characteristics between
other polyploid-derived plants and their parents. The data presented here would supply important fundamental information for
commercial mass-propagation using somatic embryogenesis.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
43.
Hirai T Nunoya T Ihara T Saitoh T Shibuya K Nakamura K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):179-182
Infectivity of porcine circovirus (PCV) 1 and PCV2 was examined in primary porcine hepatocyte culture by comparing that of PCV in primary kidney cell culture. The virus titer of PCV2-infected hepatocyte cultures was higher than that of the PCV1-infected hepatocyte cultures and the PCV-infected kidney cell cultures. The number of virus-positive cells was most abundant in PCV2-infected hepatocyte cultures as determined by immunohistochemistry and/or in situ hybridization. The results of our data suggest that PCV2 preferably infects cultured hepatocytes as observed in the liver of pigs with postweaning multisystemic wasting syndrome. 相似文献
44.
Yamamoto A Iwata A Saitoh T Tuchiya K Kanai T Tsujimoto H Hasegawa A Ishihama A Ueda S 《Veterinary immunology and immunopathology》2002,90(3-4):169-177
Feline granulocyte colony-stimulating factor (G-CSF) with an N-terminal histidine hexamer tag was expressed as inclusion bodies in E. coli. The G-CSF solubilized in 6 M guanidine solution was absorbed onto a Ni-NTA column and, after washing with decreasing concentrations of guanidine, eluted with imidazole in a soluble and apparently pure form. The activity of the recombinant feline G-CSF was 3×106 U/mg protein, as assayed by its stimulatory effect on NFS-60 cell proliferation. When a low level of purified feline G-CSF was administered once a day for two successive days to cats, the number of neutrophil increased 4-fold while the levels of other blood cell types remained virtually unchanged. Daily administration of G-CSF for a total of 11 days led to a more than 10-fold increase in neutrophils, an 8-fold increase in the number of monocytes and 2-fold increase in lymphocytes. No severe side effects or antibody production was observed in cats after administration of G-CSF. 相似文献
45.
Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization 
下载免费PDF全文
下载免费PDF全文 Thanh‐Van Nguyen Manita Wittayarat Lanh Thi Kim Do Thanh Van Nguyen Masahiro Nii Zhao Namula Toshiki Kunihara Fuminori Tanihara Maki Hirata Takeshige Otoi 《Animal Science Journal》2018,89(8):1207-1213
Electroporation is the technique of choice to introduce an exogenous gene into embryos for transgenic animal production. Although this technique is practical and effective, embryonic damage caused by electroporation treatment remains a major problem. This study was conducted to evaluate the optimal culture system for electroporation‐treated porcine embryos by supplementation of chlorogenic acid (CGA), a potent antioxidant, during in vitro oocyte maturation. The oocytes were treated with various concentrations of CGA (0, 10, 50, and 100 μmol/L) through the duration of maturation for 44 hr. The treated oocytes were then fertilized, electroporated at 30 V/mm with five 1 msec unipolar pulses, and subsequently cultured in vitro until development into the blastocyst stage. Without electroporation, the treatment with 50 μmol/L CGA had useful effects on the maturation rate of oocytes, the total cell number, and the apoptotic nucleus indices of blastocysts. When the oocytes were electroporated after in vitro fertilization, the treatment with 50 μmol/L CGA supplementation significantly improved the rate of oocytes that developed into blastocysts and reduced the apoptotic nucleus indices (4.7% and 7.6, respectively) compared with those of the untreated group (1.4% and 13.0, respectively). These results suggested that supplementation with 50 μmol/L CGA during maturation improves porcine embryonic development and quality of electroporation‐treated embryos. 相似文献
46.
Yasutomo Hori Masayuki Iguchi Yasuhiro Heishima Yohei Yamashita Kensuke Nakamura Atsushi Hirakawa Akihito Kitade Toshiki Ibaragi Michio Katagi Tamotsu Sawada Masashi Yuki Nobuyuki Kanno Haruki Inaba Noriko Isayama Hideyuki Onodera Naoki Iwasa Mikio Kino Mikihiro Narukawa Syuhei Uchida 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):922-929
47.
Nagai Emiko Iwai Miwa Koketsu Ritsuko Okuno Yoshinobu Suzuki Yuri Morimoto Ryosuke Sumitani Hidenobu Ohshima Atsushi Enomoto Toshiki Isegawa Yuji 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(4):538-543
Plant Foods for Human Nutrition - Our previous study showed anti-influenza virus activity in adlay tea prepared from adlay seeds, naked barley seeds, soybean, and cassia seeds. In this study, we... 相似文献
48.
Sato M Hosokawa T Yamaguchi T Nakano T Muramoto K Kahara T Funayama K Kobayashi A Nakano T 《Journal of agricultural and food chemistry》2002,50(21):6245-6252
Seven kinds of angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated from the hydrolysates of wakame (Undaria pinnatifida) by Protease S "Amano" (from Bacillus stearothermophilus) by using three-step high-performance liquid chromatography (HPLC) on a reverse-phase column. These peptides were identified by amino acid composition analysis, sequence analysis, and liquid chromatography-mass spectrometry (LC-MS), as Val-Tyr (IC(50) = 35.2 microM), Ile-Tyr (6.1 microM), Ala-Trp (18.8 microM), Phe-Tyr (42.3 microM), Val-Trp (3.3 microM), Ile-Trp (1.5 microM), and Leu-Trp (23.6 microM). These peptides have resistance against gastrointestinal proteases in vitro. Each peptide was determined to have an antihypertensive effect after a single oral administration in spontaneously hypertensive rats (SHR). Among them, the blood pressure significantly decreased by Val-Tyr, Ile-Tyr, Phe-Tyr, and Ile-Trp in a dose of 1 mg/kg of body weight (BW). The present study showed that antihypertensive effect in the hydrolysates of wakame by Protease S "Amano" was attributed to these peptides. 相似文献
49.
To apply otolith microstructure to examination of age and growth of juvenile chum salmon Oncorhynchus keta inhabiting coastal waters, formation of otolith increments was investigated for juveniles reared in a seawater aquarium and
in net pens. In all otoliths examined, a distinctive check was formed at the time of sea entry of the fish. The deposition
of otolith increments after the check was daily for rearing both in the aquarium (57 days) and in the net pens (26 days).
Check formation associated with sea entry was also observed in otoliths of juvenile salmon collected 1 km off the coast of
Shari, Hokkaido, Japan. Transmitted light observation of otoliths of those fish revealed a transition in otolith increment
appearance from dark to light. Otolith Sr: Ca ratio remarkably changed from a low to a high level, coinciding with the transition
in otolith appearance. It is suggested that the transition was associated with individual sea entry. This study demonstrated
that the check and/or transition associated with sea entry are applicable to a benchmark for otolith increment counts of juvenile
chum salmon inhabiting coastal waters. 相似文献
50.
Takashi Endo Masayuki Yamaguchi Ryota Kaji Koji Nakagomi Tomomori Kataoka Narifumi Yokogami Toshiki Nakamura Goro Ishikawa Jun-ichi Yonemaru Takeshi Nishio 《Breeding Science》2012,62(4):334-339
It has long been known that a bacterial leaf blight-resistant line in rice obtained from a crossing using ‘Asominori’ as a resistant parent also has resistance to blast, but a blast resistance gene in ‘Asominori’ has not been investigated in detail. In the present study, a blast resistance gene in ‘Asominori’, tentatively named Pias(t), was revealed to be located within 162-kb region between DNA markers YX4-3 and NX4-1 on chromosome 4 and to be linked with an ‘Asominori’ allele of the bacterial leaf blight resistance gene Xa1, tentatively named Xa1-as(t). An ‘Asominori’ allele of Pias(t) was found to be dominant and difference of disease severity between lines having the ‘Asominori’ allele of Pias(t) and those without it was 1.2 in disease index from 0 to 10. Pias(t) was also closely linked with the Ph gene controlling phenol reaction, suggesting the possibility of successful selection of blast resistance using the phenol reaction. Since blast-resistant commercial cultivars have been developed using ‘Asominori’ as a parent, Pias(t) is considered to be a useful gene in rice breeding for blast resistance. 相似文献