Urine hemojuvelin in cats with naturally occurring kidney disease     

Hwei Jing  Wei-Li Hsu  Vin-Cent Wu  Han-Ju Tsai  Shang-Feng Tsai  Pei-Shiue Jason Tsai  Tai-Shuan Lai  Ya-Jane Lee 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1222-1230

  相似文献   

Substitution of Chaetoceros muelleri by Spirulina platensis meal in diets for Litopenaeus schmitti larvae     

Barbarito J. Jaime-Ceballos  Alfredo Hernndez-Llamas  Tsai Garcia-Galano  Humberto Villarreal 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):215-220

The nutritional response of Litopenaeus schmitti larvae to substitution of Chaetoceros muelleri by Spirulina platensis meal (SPM) was evaluated. The substitution levels (S) were 0%, 25%, 50%, 75% and 100%, dry weight basis. Final larval length (FL) ranged from 1.98 to 3.16 mm for the different substitution levels. There was a significant relationship between S and FL, described by the following quadratic equation: FL = 2.853 + 0.01598S − 0.000233S². The substitution level (S) yielding maximum FL was 34.2%. Development index (DI) values ranged from 2.84 to 3.93 and were dependent on substitution level. The corresponding equation was DI = 3.799 + 0.00945S − 0.000189S² (P < 0.01). Maximum DI was obtained at 25.0% substitution. Survival was high (82–87%) and no significant differences were found between treatments. Protein digestibility of either microalgae was high, with 92% for SPM and 94% for C. muelleri, with no significant differences between them. The results in this study indicate that an adequate balance of nutrients in relation to the requirements of the species is critical. To simultaneously improve FL and DI, a 30% substitution of C. muelleri by SPM is suggested. This is equivalent to feeding 0.15 mg larvae^− 1 day^− 1 dry weight basis of a 70% C. muelleri/30% SPM diet, representing 0.078 mg protein larvae^− 1 day^− 1, 0.026 mg lipids larvae^− 1 day^− 1 and 2.732 J larvae^− 1 day^− 1.  相似文献   

Genetic Characterization of Brazilian Annual <Emphasis Type="Italic">Arachis</Emphasis> Species from Sections <Emphasis Type="Italic">Arachis</Emphasis> and <Emphasis Type="Italic">Heteranthae</Emphasis> using RAPD Markers     

Silvana?CresteEmail author  Siu?Mui?Tsai  José?F.?M.?Valls  Marcos?A.?Gimenes  Catalina?Romero?Lopes 《Genetic Resources and Crop Evolution》2005,52(8):1079-1086

The genus Arachis is divided into nine taxonomic sections. Section Arachis is composed of annual and perennial species, while section Heteranthae has only annual species. The objective of this study was to investigate the genetic relationships among 15 Brazilian annual accessions from Arachis and Heteranthae using RAPD markers. Twenty-seven primers were tested, of which nine produced unique fingerprintings for all the accessions studied. A total of 88 polymorphic fragments were scored and the number of fragments per primer varied from 6 to 17 with a mean of 9.8. Two specific markers were identified for species with 2n = 18 chromosomes. The phenogram derived from the RAPD data corroborated the morphological classification. The bootstrap analysis divided the genotypes into two significant clusters. The first cluster contained all the section Arachis species, and the accessions within it were grouped based upon the presence or absence of the ‘A’ pair and the number of chromosomes. The second cluster grouped all accessions belonging to section Heteranthae.  相似文献   

Seasonal Changes in Cystoseira Myrica and Phosphorus Input at Two Sites of the Red Sea Egyptian Coast     

Abou-Aisha  KH. M.  Shabana  Effat F.  El-Abyad  M. S.  Kobbia  I. A.  Schanz  F. 《Water, air, and soil pollution》1997,93(1-4):199-211

Elevated phosphate concentrations at Safaga and Quseir, Red Sea, Egyptian coast, wererelated to some ecological and nutritional aspects of the macro-alga Cystoseira myrica during1985. The results obtained were compared with those recorded for a non-polluted control site atGhardaqa. Chemical analysis of surface seawater samples revealed that the concentrations of dissolvedphosphate at Safaga and Quseir were 3 and 20 times as much as that at Ghardaqa, respectively.The standing crop of C. myrica showed two peaks in spring and autumn. Maximum biomass yield(491 g m-2) and number of individuals (127 m-2) of C. myrica were recorded in the spring at theGhardaqa site. Quseir C. myrica samples contained significantly higher protein-N, total-N andtotal-P values but lower carbohydrate values than the Safaga and Ghardaqa samples (LSD at 1%level). Tissue N:P molar ratios for C. myrica, Laurencia papillosa and Ulva lactuca collected fromGhardaqa ranged from 68-98, compared to 30-59 for the same species collected at thephosphorus polluted sites at Safaga and Quseir. The carbon levels and the C:N ratios of C. myricaexhibited no significant variations in the three studied sites. However, the C:P ratios of Ghardaqaplants were significantly higher than those of Safaga and Quseir.  相似文献   

Quercetin and rutin reduced the bioavailability of cyclosporine from Neoral, an immunosuppressant, through activating P-glycoprotein and CYP 3A4     

Yu CP  Wu PP  Hou YC  Lin SP  Tsai SY  Chen CT  Chao PD 《Journal of agricultural and food chemistry》2011,59(9):4644-4648

Quercetin and rutin are popular flavonoids in plant foods, herbs, and dietary supplements. Cyclosporine (CSP), an immunosuppressant with a narrow therapeutic window, is a substrate of P-glycoprotein (P-gp) and cytochrome P-450 3A4 (CYP3A4). This study investigated the effects of quercetin and rutin on CSP pharmacokinetics from Neoral and relevant mechanisms. Rats were orally administered Neoral with and without quercetin or rutin. The blood CSP concentration was assayed by a specific monoclonal fluorescence polarization immunoassay. The results showed that quercetin and rutin significantly decreased the C(max) of CSP by 67.8 and 63.2% and reduced the AUC(0-540) by 43.3 and 57.2%, respectively. The in vitro studies indicated that the quercetin and rutin induced the functions of P-gp and CYP3A4. In conclusion, quercetin and rutin decreased the bioavailability of CSP through activating P-gp and CYP3A. Transplant patients treated with Neoral should avoid concurrent consumption of quercetin or rutin to minimize the risk of allograft rejection.  相似文献   

Structure and function relationship study of allium organosulfur compounds on upregulating the pi class of glutathione S-transferase expression     

Tsai CW  Liu KL  Lin CY  Chen HW  Lii CK 《Journal of agricultural and food chemistry》2011,59(7):3398-3405

Allium organosulfides are potential chemopreventive compounds due to their effectiveness on the induction of phase II detoxification enzyme expression. In this study, we examined the structure and function relationship among various alk(en)yl sulfides on the expression of the pi class of glutathione S-transferase (GSTP) in rat Clone 9 cells, and what mechanism is involved. Cells were treated with 300 μM dipropyl sulfide (DPS), dipropyl disulfide (DPDS), propyl methyl sulfide (PMS), and propyl methyl disulfide (PMDS) for 48 h. DPDS and PMDS displayed more potency on GSTP protein and mRNA induction than that of DPS and PMS. Next, we compared the effectiveness of DPDS, PMDS, and diallyl disulfide (DADS), which have the same number of sulfur atoms but differ in the side alk(en)yl groups. The maximum increases on protein expression, mRNA level, and enzyme activity were noted in cells treated with DADS, followed by DPDS and PMDS. A reporter assay showed that three disulfides increased GSTP enhancer I (GPE I) activity (P < 0.05) in the order DADS > DPDS ≥ PMDS. Electromobility gel shift assays showed that the DNA binding of GPE I to nuclear proteins reached a maximum at 1 to 3 h after alk(en)yl disulfide treatment. Supershift assay revealed that c-jun bound to GPE I. Silencing of extracellular signal-regulated kinase (ERK) 2 expression inhibited c-jun activation and GSTP induction. Results suggest that both the type of alk(en)yl groups and number of sulfur atoms are determining factors of allium organosulfides on inducing GSTP expression, and it is likely related to the ERK-c-Jun-GPE I pathway.  相似文献   

Effect of nanogrinding on the pigment and bioactivity of Djulis ( Chenopodium formosanum Koidz.)     

Tsai PJ  Chen YS  Sheu CH  Chen CY 《Journal of agricultural and food chemistry》2011,59(5):1814-1820

Betanin is an antioxidant pigment found in djulis, a grain native to Taiwan, and is a natural source food coloring, but the bright red color degrades rapidly if submitted to light, heat, or oxygen. The effects of nanogrinding on the stability of pigments and bioactive components are unknown. In this study, the color characteristics and bioactivity (antioxidant capacity and enzyme activity) of nanoparticle (NP) djulis was compared with those of intact granules (IG) and microparticles (MP). Results showed that the NP samples exhibited the highest betanin content (2.04 mg/g), which was almost twice that of IG. It was observed that nanogrinding resulted in higher pigment extraction efficiency. However, during storage (5-35 °C for 56 days), NP samples showed the most serious pigment degradation, and this color degradation, as expected, had the lowest activation energy. This was more evident when the storage temperature was high. Antioxidant capacities showed the same trends. MP and NP exhibited significantly higher activity of superoxide dismutaste-like activity, lactoperoxidase (LPO), and lysozyme than IG. Gel permeation chromatography confirmed the degradation of larger particles during nanogrinding, which might favor enzyme extraction and their activities. Statistical analysis revealed a close relationship between betanin and antioxidant capacity.  相似文献   

Genetic variation in resistance of turkeys to experimental infection with Newcastle disease virus.     

H J Tsai  Y M Saif  K E Nestor  D A Emmerson  R A Patterson 《Avian diseases》1992,36(3):561-565

Seven hundred eighty male and female turkeys representing four genetic lines were challenged in four experiments with the Texas GB strain of Newcastle disease virus (NDV). The lines of turkeys included two randombred control lines (RBC1 and RBC2), a subline (E) of RBC1 selected for increased egg production, and a subline (F) of RBC2 selected for increased 16-week body weight. Mortality in turkeys of subline F (32.5%) was significantly higher than that in turkeys of line RBC2 (15.8%), subline E (17.5%), and line RBC1 (18.4%). At the end of each experiment, surviving birds were tested for antibody to NDV using the enzyme-linked immunosorbent assay (ELISA) and hemagglutination-inhibition (HI) test. Turkeys of subline E and line RBC1 had significantly lower ELISA antibody titers than those of subline F and line RBC2. Subline F had the highest HI antibody titers, followed in decreasing order by lines RBC2 and RBC1 and subline E. No apparent correlation was found between antibody response and mortality after NDV challenge.  相似文献   

Identification of a juvenile hormone-like compound in a crustacean   总被引：3，自引：0，他引：3  

Laufer H  Borst D  Baker FC  Reuter CC  Tsai LW  Schooley DA  Carrasco C  Sinkus M 《Science (New York, N.Y.)》1987,235(4785):202-205

Juvenile hormone (JH) has central roles in the regulation of insect development and reproduction but has not previously been identified in other arthropod classes. The hemolymph of a crustacean, Libinia emarginata (Leach), has now been analyzed for JH-like compounds. Samples contained 0.003 to 0.030 nanogram of JH III per milliliter and 10 to 50 nanograms of methyl farnesoate per milliliter; methyl farnesoate is a compound structurally related to JH III that has JH bioactivity. Several tissues were examined for synthesis and secretion of JH-like compounds. Of these tissues, only the mandibular organs produced and secreted JH III and methyl farnesoate. However, microchemical analysis revealed that this JH III was racemic, and thus likely an artifactual oxidation product of methyl farnesoate. Secretion of methyl farnesoate was related to reproduction in females, with the highest rates observed in Libinia near the end of the ovarian cycle when oocyte growth and vitellogenesis are greatest. These results indicate that JH-like compounds such as methyl farnesoate have regulatory roles in crustaceans.  相似文献   

Starch-deficient maize mutant lacking adenosine dephosphate glucose pyrophosphorylase activity   总被引：24，自引：0，他引：24  

C Y Tsai  O E Nelson 《Science (New York, N.Y.)》1966,151(708):341-343

The maize mutant shrunken-2 synthesizes only 25 to 30 percent as much starch as normal maize; it completely lacks adenosine diphosphate glucose pyrophosphorylase activity in both endosperm and embryo tissue. Identification of the mutant block indicates that the greater portion of starch in the endosperm of normal maize is synthesized by way of enzyme systems that utilize adenosine diphosphate glucose as a substrate, and that the latter is formed chiefly by adenosine diphosphate glucose pyrophosphorylase.  相似文献