Comparison of growth and endocrine changes in Thoroughbred colts and fillies reared under different climate conditions     

Hirotoshi MIZUKAMI  Tsuyoshi SUZUKI  Yasuo NAMBO  Mutsuki ISHIMARU  Hiroshi NAITO  Kenji KOROSUE  Kentaro AKIYAMA  Kenji MIYATA  Akira YAMANOBE  Kentaro NAGAOKA  Gen WATANABE  Kazuyoshi TAYA 《Journal of Equine Science》2015,26(2):49-56

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Administration of two oils rich in n-3 long-chain polyunsaturated fatty acids to rat pups of dams fed a diet high in fat and low in n-3 polyunsaturated fatty acids     

Fumiko?KimuraEmail author  Yasushi?Endo  Kenshiro?Fujimoto  Nobushige?Doisaki  Tsuyoshi?Koriyama 《Fisheries Science》2005,71(2):431-440

Long-chain polyunsaturated fatty acids (LCPUFA) with 20 or 22 carbons are considered important to the development of infants and sometimes added to infant formulae. In this study, two characteristic sources of n-3 LCPUFA (fish oil and microalgal oil) were orally administrated to rat pups of mildly n-3 PUFA — deficient dams to compare the consequences of the administration. The milk from the dams fed a n-3 PUFA — restricted diet contained less n-3 LCPUFA than that of the dams fed a control diet. Pups were administered 1 mg/g weight of the test oil at the age of 5–7 days. At the age of 7 days, they were sacrificed before or after the administration and fatty acid compositions of the stomach and serum lipid were studied. The administration changed docosahexaenoic acid (DHA; 22:6n−3) levels in the stomach contents and serum lipids with time. Eicosapentaenoic acid (EPA; 20:5n−3) levels increased immediately after the administration of fish oil. The administration of microalgal oil also affected the serum lipid EPA level, in spite of a lack of EPA. In this study, both oils effectively supplemented DHA. Fish oil returned the serum EPA level close to the control value while microalgae oil had little effect.  相似文献   

Oleosome-Associated Protein of the Oleaginous Diatom Fistulifera solaris Contains an Endoplasmic Reticulum-Targeting Signal Sequence     

Yoshiaki Maeda  Yoshihiko Sunaga  Tomoko Yoshino  Tsuyoshi Tanaka 《Marine drugs》2014,12(7):3892-3903

Microalgae tend to accumulate lipids as an energy storage material in the specific organelle, oleosomes. Current studies have demonstrated that lipids derived from microalgal oleosomes are a promising source of biofuels, while the oleosome formation mechanism has not been fully elucidated. Oleosome-associated proteins have been identified from several microalgae to elucidate the fundamental mechanisms of oleosome formation, although understanding their functions is still in infancy. Recently, we discovered a diatom-oleosome-associated-protein 1 (DOAP1) from the oleaginous diatom, Fistulifera solaris JPCC DA0580. The DOAP1 sequence implied that this protein might be transported into the endoplasmic reticulum (ER) due to the signal sequence. To ensure this, we fused the signal sequence to green fluorescence protein. The fusion protein distributed around the chloroplast as like a meshwork membrane structure, indicating the ER localization. This result suggests that DOAP1 could firstly localize at the ER, then move to the oleosomes. This study also demonstrated that the DOAP1 signal sequence allowed recombinant proteins to be specifically expressed in the ER of the oleaginous diatom. It would be a useful technique for engineering the lipid synthesis pathways existing in the ER, and finally controlling the biofuel quality.  相似文献   

Correction to: First detection of two potyviruses,uraria mosaic virus and passiflora foetida virus Y,from passionfruit in Japan     

Nakasato  Kota  Fujioka  Sayaka  Sugawara  Yuuji  Ono  Tsuyoshi  Nishio  Takeshi  Tsuda  Shinya 《Journal of General Plant Pathology》2022,88(2):160-160

Journal of General Plant Pathology -  相似文献   

First report of horn gall disease of Cinnamomum pseudopedunculatum caused by Laurobasidium hachijoense in the Bonin Islands,Japan     

Shibata  Saho  Ono  Tsuyoshi  Kobayashi  Yoshiko  Miyagawa  Itsuha  Mukai  Akitsugu  Ichioka  Youzou  Takenaka  Izumi  Nakano  Hideto  Hirooka  Yuuri 《Journal of General Plant Pathology》2021,87(5):322-325

Journal of General Plant Pathology - In June 2017, deer-horn-like galls covered with a whitish, powdery fungal layer were found on the trunks of Cinnamomum pseudopedunculatum (Lauraceae) in...  相似文献   

Evaluation of the concentrations of hydrogen and methane emitted by termite using a semiconductor gas sensor     

Yoshiyuki Yanase  Masahiro Miura  Yoshihisa Fujii  Shogo Okumura  Tsuyoshi Yoshimura 《Journal of Wood Science》2013,59(3):243-248

A gas detection apparatus equipped with a semiconductor gas sensor was employed for qualitative and quantitative measurement of hydrogen and methane emitted by termites. A gas sample of 2.5 ml was injected into the semiconductor gas sensor through the gas detection apparatus, and the maximum voltage of the sensor was converted into gas concentration. The gas samples were collected from three distinct experiments: (1) five combinations of workers and soldiers of Coptotermes formosanus with and without a wood specimen; (2) C. formosanus under six temperature conditions; and (3) four different termite species, C. formosanus, Reticulitermes speratus, Incisitermes minor, and Zootermopsis nevadensis. The hydrogen and methane concentrations increased with an increase in the number of termites. Concentrations were higher in samples with a wood specimen than without it. Both hydrogen and methane concentrations were the highest for the samples at 35 °C and were lowest at 15 and 5 °C. The concentrations were very low at 45 °C because all the termites had died in a few hours. The concentrations of hydrogen and methane were highest for Z. nevadensis, the dampwood termite, among the four species, and no methane was detected for I. minor, the drywood termite, at 28 °C and 75 % RH.  相似文献   

bex-db: Bioinformatics workbench for comprehensive analysis of barley-expressed genes     

Tsuyoshi Tanaka  Hiroaki Sakai  Nobuyuki Fujii  Fuminori Kobayashi  Shingo Nakamura  Takeshi Itoh  Takashi Matsumoto  Jianzhong Wu 《Breeding Science》2013,63(4):430-434

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Exogenous ascorbic acid derivatives and dehydroascorbic acid are effective antiviral agents against Turnip mosaic virus in Brassica rapa     

Ayaka Fujiwara  Hanako Shimura  Chikara Masuta  Shinsuke Sano  Tsuyoshi Inukai 《Journal of General Plant Pathology》2013,79(3):198-204

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Tomatidine, a tomato sapogenol, ameliorates hyperlipidemia and atherosclerosis in apoE-deficient mice by inhibiting acyl-CoA:cholesterol acyl-transferase (ACAT)     

Fujiwara Y  Kiyota N  Tsurushima K  Yoshitomi M  Horlad H  Ikeda T  Nohara T  Takeya M  Nagai R 《Journal of agricultural and food chemistry》2012,60(10):2472-2479

It was previously revealed that esculeoside A, a new glycoalkaloid, and esculeogenin A, a new aglycon of esculeoside A, contained in ripe tomato ameliorate atherosclerosis in apoE-deficent mice. This study examined whether tomatidine, the aglycone of tomatine, which is a major tomato glycoalkaloid, also shows similar inhibitory effects on cholesterol ester (CE) accumulation in human monocyte-derived macrophages (HMDM) and atherogenesis in apoE-deficient mice. Tomatidine significantly inhibited the CE accumulation induced by acetylated LDL in HMDM in a dose-dependent manner. Tomatidine also inhibited CE formation in Chinese hamster ovary cells overexpressing acyl-CoA:cholesterol acyl-transferase (ACAT)-1 or ACAT-2, suggesting that tomatidine suppresses both ACAT-1 and ACAT-2 activities. Furthermore, the oral administration of tomatidine to apoE-deficient mice significantly reduced levels of serum cholesterol, LDL-cholesterol, and areas of atherosclerotic lesions. The study provides the first evidence that tomatidine significantly suppresses the activity of ACAT and leads to reduction of atherogenesis.  相似文献   

The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus     

Naoaki Tsutsui  Ayano Nagakura-Nakamura  Chiaki Nagai  Tsuyoshi Ohira  Marcy N. Wilder  Hiromichi Nagasawa 《Fisheries Science》2013,79(1):33-38

Seven major peptides belonging to the crustacean hyperglycemic hormone family were purified from the sinus gland located in the eyestalk of the kuruma prawn Marsupenaeus japonicus, and their effects on vitellogenin gene expression were examined using the ex vivo ovary incubation system. Six molecular species of crustacean hyperglycemic hormone, Pej-SGP-I, -II, -III, -V, VI, and VII, displayed significant inhibitory effects on vg expression with almost the same efficacies, whereas Pej-SGP-IV (known as molt-inhibiting hormone) did not. Two chromatophorotropic peptides, red pigment-concentrating hormone and pigment-dispersing hormone, which were also present in the sinus glands, did not have a clear effect on the gene expression levels in this incubation system. These results suggest that the six crustacean hyperglycemic hormones are potentially capable of acting as vitellogenesis-inhibiting hormones in M. japonicus.  相似文献